Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

간호정보의 처리, 분석, 관리 기술개발-인공신경망을 이용한 간호진단시스템 구현-

유지수,유황빈,박지원,고일선 대한의료정보학회 1998 Healthcare Informatics Research Vol.4 No.2

충남지역 S대학의 중앙도서관과 휴게시설의 총부유분진 및 총부유세균 농도 연구

이서빈,김지욱,임채령,이정민,정소진,이민혁,손부순 순천향대학교 기초과학연구소 2021 순천향자연과학연구 논문집 Vol.27 No.1.2

The average indoor concentration of the library's TSP was 0.03 ng/m³. TAB in library and lounge in college of engineering showed higher concentrations in corridors than indoors. The correlation coefficient between TAB and humidity is 0.985, so it is judged that there is a significant need for humidity control in indoor air quality management to reduce the concentration of total suspended bacteria(p<0.01).

디지털 I-PD 제어기에 의한 태양광 발전 시스템의 정출력 제어

박지호,김영빈,신동률,문백영,우정인 東亞大學校 大學院 2000 大學院論文集 Vol.25 No.-

In this paper, constant output control of solar power generation system with instantaneous variable output is proposed. In order to get constant DC and AC voltage source, we construct Buck-Boost chopper equip with the digital I-PD type controller first. Consequently, we are able to get constant DC boltage. Next, we obtain AC source through construction PWM single phase inverter that has the constant frequency output. And we design LC filter to reduce THD(Total Harmonic Distortion). The validity of proposed control strategy is verified from results of simulation.

인터넷 배너광고의 유형에 따른 광고 효과

오지은,김성희,이상빈 전북대학교 사회과학연구소 2003 지역과 세계 Vol.29 No.-

This study was to investigate the effect of internet banner types(texts, animations, sexy models) on indexes of advertising effects(attitude toward the advertisement( A_ad), brand attitude( A_b), purchase intention(PI)]. In this study, 180 subjects were conveniently sampled, and randomly assigned to one of 6 conditions in 3(ad types) × 2(products types) factorial design. The major findings of this study as follows. The results of A_ad on differences compared with the ad using texts and sexy models. And the ad for computers had statistically significant differences from ad for books. The results of A_b on ad types had significant main effects, but the results of A_b on products types and interaction effects had no significant differences. The ad using texts and animations had statistically significant differences from the ad using sexy models. The results of PI on ad types had significant main effects, but the results of PI on products types and interaction effects had no significant differences. The ad using texts and animations had statistically significant differences from the ad using sexy models. Finally, implications and limitations of this study were discussed.

국내 시범운영 가상대학들의 운영체계 평가에 관한 연구

차석빈,오지연 순천향대학교 사회과학연구소 2000 순천향 사회과학연구 Vol.6 No.1

As people need the lifelong education in the 21st century, our society launched cyber education as a new education paradigm two years ago. This study attempted to evaluate the operation system of five cyber demonstration universities in Korea. Research finding indicate that there leave room for improvement ion several aspects. This study suggests that cyber education in the future need to consolidate the 'humanware', 'hardware', 'software' and ' baseware' to function effectively as well as to solve the problems that have been appeared during the last two-year demonstration period.

상악 정중과잉치의 발병율

김종빈,김종수,박지은 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

2003년 4월부터 2006년 4월까지 안양 지역에 위치한 개인 소아치과 병원에 내원한 환아 7038명을 대상으로 임상적,방사선학적 검사를 통해 성별,상악 정중과잉치의 수,치관의 형태와 위치,치관 방향을 조사하여 다음과 같은 결론을 얻었다. 1. 과잉치를 가진 환아는 239명으로 3.40%(총 241명. 3.42%)에 해당하였고 총 289개의 과잉치가 있었다. 2. 과잉치는 1개 있는 경우가 191명으로 가장 많았고, 2개 있는 경우도 48명 있었으며 3개 이상 있는 경우는 없었다. 3. 상악 정중과잉치는 3.2:1로 여아보다 남아에서 호발하였다. 4. 과잉치는 왼쪽에 있는 경우가 55.4%로 약간 더 많았고,모양은 원추형,방향은 역위형이 가장 많았다. 5. 하악 정중과잉치는 2명에서 관찰되었다. The purpose of this study was to evaluate the prevalence of mesiodens at pediatric dental clinic in Anyang, Kyeong Gi-Do. From retrospective reviews of all patients who visited Kizwell pediatric dental clinic from 2003.4-2006.4, 241 patients (289 mesiodens) who were shown to have mesiodentes were examed by a periapical radiograph and a panoramic radiograph. The incidence of mesiodens was 3.42% and the ratio of male/female (184/57) was 3.2:1. The number of supernumerary teeth was one in 193 cases (80%), two in 48 cases (20%). The mesiodens was placed at left side more frequently and the most common shape was conical type (208, 78%). Of 289 mesiodens, the direction of the crown of the mesiodens was inverted in 156 (54%), in a normal direction in 104 (36%), in a horizontal direction with regard to the tooth axis in 29 (10%). Early diagnosis of the mesiodens allow the most appropriate treatment, often reducing the extent of surgery, orthodontic treatment and possible complications.

국내 의료기관의 항생제 사용 실태와 규제 시스템 조사

방지환,송경호,박완범,김성한,조재현,김홍빈,김남중,오명돈,최강원 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

목적 : 이전 연구들에 따르면 아직까지 국내에서 불필요한 항생제 사용은 무시할 수 없는 수준이며, 특히, 수술환자들에 대한 예방적 항생제 투여가 문제로 지적되고 있다. 많은 병원에서 불필요한 항생제 처방을 제한하려는 노력을 하고 있지만 구체적인 현황에 대한 자료는 적은 실정이다. 이에 본 연구자 등은 국내 의료기관의 항생제 규제 시스템의 현황과 수술시 예방적 항생제 사용의 적정성을 알아보고자 한다. 재료 및 방법 : 전임 감염내과 전문의가 근무하는 의료기관 55곳에 대해 설문조사를 시행하였다. 설문의 내용은 적정한 항생제 사용을 유도하기 위한 정책 및 규제 프로그램, 실제 각 의료기관의 예방적 항생제 사용 현황 등으로 이루어져 있다. 결과 : 55개의 의료기관에 보낸 설문 중 44개가 회수되었다. 회신한 의료기관에서 항생제 처방 관리는 제한항생제 시스템을 가장 많이 이용하고 있었고(95.5%), 항생제에 대한 교육(79.5%), 전산시스템에서 관리(59.1%), 감염내과에 의뢰(54.5%), 항생제 사용 실적 검토(52.3%) 등의 순이었다. 대개의 병원에서 glycopeptides (100.0%), carbapenems (93.0%), quinupristin/dalfopristin 또는 linezolid(93.0%), 4세대 cephalosporin (74.4%), caspofungin 또는 voriconazole (62.8%), liposomal amphotericin B (60.5%) 등을 제한항생제로 분류하여 관리하고 있었다. 모든 의료기관에서 위절제술 및 유방절제술시에 불필요하게 예방적 항생제를 사용하고 있었다. 슬관절치환술의 경우 2세대 cephalosporin을 예방적으로 사용하는 경우가 가장 많았으며(30.2%), 관상동맥우회술의 경우 3세대 cephalosporin을 예방적으로 사용하는 경우가 가장 많았다(47.2%). 예방적 항생제의 투여 기간을 살펴보면 위절제술시 70.5%에서 4일을 초과하여 사용하고 있었고, 유방절제술시에는 63.6%에서, 슬관절치환술시에는 81.8%에서, 관동맥우회술시에는 81.1%에서 4일을 초과하여 장기간 예방적 항생제를 투여하고 있었다. 한편, 예방적 항생제 투여시 aminoglycoside계 항생제를 병용하는 경우도 많았다. 결론 : 아직까지 국내 의료기관에서 항생제 오남용을 막기 위한 노력이 더 필요할 것으로 생각되며, 특히 수술시 예방적 항생제의 올바른 투여에 많은 관심이 필요하다. Background : Previous studies in Korea showed that unnecessary antibiotic use could not be neglected, especially in prophylactic use. Currently many hospitals employ diverse antibiotic stewardship programs, but there are few studies on surveillance of such programs. So, we planned this study to evaluate current status of antimicrobial stewardship and adequacy of antimicrobial prophylaxis in surgery. Materials and Methods : We sent questionnaires about policy on antibiotic usage, control program for adequate antibiotic prescription, and actual status of prophylactic antibiotic usage to 55 infectious disease physicians in each hospital. Results : Of 55 contacted infectious diseases clinicians, 44 answered the questionnaire. Majority of hospitals employed restricted antimicrobial system (95.5%), education (79.5%), control by order communicating system (59.1%), consult to infectious disease physicians (59.1%), and review of cumulative antimicrobial usage (52.3%) to prevent inadequate antibiotic use. Glycopeptides were designated as a restricted antimicrobial agent in 100.0% of hospitals; carbapenems, 93.0%; quinupristin/ dalfopristin or linezolid, 93.0%; the 4th generation cephalosporins, 74.4%; caspofungin or voriconazole, 62.8%; liposomal amphotericin B, 60.5%, and so on. Unnecessary perioperative prophylactic antimicrobial agents were prescribed in gastrectomy and mastectomy in all the hospitals. The second generation cephalosporins and the third generation cephalosporins were the most commonly prescribed prophylactic antimicrobial agents in total knee replacement arthroplasty (TKRA) and coronary artery bypass graft surgery (CABG) respectively. In 70.5% of institutes they use prophylactic antimicrobial agents more than four days in gastrectomy; 63.6% in mastectomy; 81.8% in TKRA; 81.1% in CABG. Unnecessary combination of aminoglycosides with other antimicrobial agents for prophylactic use was another common problem. Conclusion : This study shows that more effort is required to diminish antimicrobial misuse or overuse, especially in prophylactic use for surgical patients.

외란관측기를 사용한 복합형능동전력필터의 Deadbeat제어에 관한 연구

우정인,박지호,김영빈,문백영 東亞大學校 2000 東亞論叢 Vol.37 No.-

When LC filter is connected parallel to the Active Power Filter, a problem of the resonance between the Active Power Filter(AF) and LC filter often arises. In this paper, deadbeat control of Hybrid Active Power Filter using Disturbance-observer is introduced to solve this problem. By the estimation of the harmonics disturbance using proposed disturbance observer, the compensation characteristics of the AF is improved and the resonant current is suppressed. The validity of proposed disturbance observer is verified from simulation results using PSIM.

색상 측정 기기를 이용한 복합레진 적층 수복과 단일 수복의 색상 비교 분석

송영상,김자현,이빈나,장지현,장훈상,황윤찬,오원만,황인남 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2

Objectives: This study analyzed the difference in color caused by different thickness in enamel layer of composite resins when applied with single and layering placement technique, and evaluated if the results agreed with the shade guide from the manufacturers to verify reliability of the color matching process of the manufacturers. Materials and Methods: For single composite resin samples, 6 mm diameter and 4 mm thickness cylindrical samples were fabricated using Ceram-X mono (DENTSPLY DeTrey) and CIE L*a*b* values were measured with spectrophotometer. Same process was done for layering compositie resin samples, making 3 dentinal shade samples, 4 mm thickness, for each shade using Ceram-X duo (DENTSPLY DeTrey) and enamel shade resins were layered in 2 mm thickness and CIE L*a*b* values were measured. These samples were ground to 0.2 mm thickness each time, and CIE L*a*b* values were measured to 1 mm thickness of enamel shade resin. Results: Color difference (△E*) between single and layering composite resin was 1.37 minimum and 10.53 maximum when layering thicknesses were between 1 mm and 2 mm and 6 out of 10 same shade groups suggested by manufacturer showed remarkable color difference at anythickness (△E* > 3.3). Conclusion: When using Ceram-X mono and duo for composite resin restoration, following the manufacturer's instructions for chossing the shade is not appropriate, and more accurate information for Ceram-X duo is needed on the variation and expression of the shades depending on the thickness of the enamel. (Restor Dent Endod 2012;37(2):84-89)