Effect of p-Phenylenediamine Application to Rat Skin on the Liver Oxygen Free Radical Systems

윤종국,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.2

To evaluate the influence of hepatic oxygen free radical systems on liver injury by topical p-phenylenediamine (PPD) application on rat skin. PPD (25mg/16.5㎠) was topically applied to the abdominal region 5 times every other day and sacrificed. By PPD treatment, increasing rate of liver weight/body weight(%), serum activities of alanine aminotransferase and aspartate aminotransferase and decreasing rate of microsomal glucose-6-phosphatase activity were higher in the rats fed tungstate supplemented diet than those fed a standard diet. These findings indicate that group fed tungstate supplemented diet have more severe liver injury compared with group fed standard diet on topical PPD application. However, the activities of oxygen free radical generating enzymes such as xanthine oxidase (XO) and cytochrome P450 dependent aniline hydroxylase and those of oxygen free radical scavenging enzymes were not found to be different between these two animal groups. In the present study, a novel monitoring method to detect the generating of oxygen free radicals in liver extract was devised. Throughout this method, the oxidized PPD produced by oxygen free radicals was determined colorimetrically. The increasing rate of PPD oxidation by liver homogenate was higher in tungstate fed animals than in standard diet fed ones. Among the fractionations of liver extract, the mitochondria1 and postmitochondrial fractions in the liver extract of tungstate fed animals led to a higher availability of PPD oxidation by PPD treatment compared with standard diet fed ones. In conclusion, these results suggest that an enhanced liver injury in tungstate fed animals treated with PPD may be due to oxygen free radicals produced in other systems except oxygen free radicals generating from cytosolic XO system. Especially, oxidative availability by PPD can be used for oxygen free radical detection in some tissue.

Saccharomyces uvarum의 인지질 생합성 및 지방산 조성에 미치는 탄소원의 효과에 관하여

이종삼,김선혜 성신여자대학교기초과학연구소 1988 基礎科學硏究誌 Vol.5 No.-

Saccharomyces uvarum을 30℃에서 Knopp's 최소배지에 자당결핍과 자당고농도 처리(15%)을 하여 배양시켰다. 이들 세포의 생장과 인지질 및 지방산 조성에 미치는 탄소원의 효과를 조사하기 위하여 배양 기간중 4, 7일 간격으로 일정량의 세포를 수확하여 생장률과 이에 따른 함량을 조사하였다. 자당 고농도 처리구는 배양 4일째에 생장감소 효과를 나타내었으며, 자당결핍구는 배양기간동안 세포생장을 관찰할 수 없었다. 주요 인지질의 지방산 palmitic acid와 oleic acid로 주로 이용되었으며, PC, PE에서는 대조구에 비하여 oleic acid가 조성상 급격한 이용의 증가를 나타내었다. Saccharomyces uvarum was cultured in the Knopp's media of the sucrose absence and the sucrose excess. In order to observe the effect of sucrose as carbon source on biosynthesis of phospholipids in these cells and the compositions of fatty acids, the cells were harvested at 4 and 7 days intervals of cultivations after inoculation. The growth rate at sucrose excess was decreased at 4 days of cultivation, but the growth rate of sucrose absence was not increase during whole period of cultivation. The fatty acids in major phospholipids were composed of palmitic acid and oleic acid. The amount of oleic acid in phosphatidylethanolamine and phosphatidylcholine was markedly increased to compared with those of the control.

흰쥐 성장기간에 따른 Xylene의 독성에 관한 연구

이혜자,이상희,전태원,이상일,윤종국 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.3

실험동물에 있어서 연령 차이에 따라서 xylene 독성이 어떠한 차이가 있는지를 검토하는 일환으로 5주령 및 12주령 흰쥐에 50% m-xylene을 체중 100 g 당 0.25 ml씩 1회 투여한 다음 24시간 후에 처치하여 다음과 같은 결과를 얻었다. Xylene 투여로 인한 요 중 methylhippuric acid 함량은 5주령군이 12주령군에 비해 현저하게 높게 나타났다. 그리고 간조직 중 cytochroms P-450 함량은 대조군에 있어서 5주령군이 12주령군 보다 약 50% 정도 낮게 나타났으나 xylene 투여로 인한 cytochrome P-450 함량 증가율은 12주령군 보다 5주령군에서 높게 나타났다. 간 alcohol dehydrogenase 활성치도 대조군에 있어서는 5주령군이 12주령 보다 약 35% 정도 낮게 나타났으나 xylene 투여로 인한 본 효소의 활성 증가율은 5주령군에서 오히려 높게 나타났다. 그러나 간 aldehyde dehydrogenase 활성치는 대조군 및 xylene 투여군 모두 5주령과 12주령간에 유의한 차이를 나타내지 않았다. 한편 xylene 투여시 체중 당 간무게, 간조직 malondialdehyde 함량 및 혈청 ALT 활성 변동을 통하여 간손상 정도를 상호 비교 관찰하였을 때, 12주령군이 5주령 실험동물 보다 간손상이 다소 심하게 나타남을 알 수가 있었다. 이상 실험결과는 연령에 따라 xylene에 의한 간손상의 차이는 이물질의 생체내 대사율이 달리 나타나기 때문일 것으로 생각된다. To evaluate an effect of aging on the xylene toxicity, 50% m-xylene in olive oil (0.25 ml/100 g body wt.) was administered to 5 week and 12 week-old rats one times intraperitoneally and sacrificed at 24 hrs afterwards. The increasing rate of urinary H-methylhippuric acid concentration was higher in 12 week-old rats than 5 week-old rats by the treatment of m-xylene. On the liver function findings, i.e., liver weight/body weight (%), serum levels of ALT activity and hepatic malondialdehyde content, 12 week-old rats showed more severe liver injury than 5 weeks those in xylene-treated rats. And the hepatic cytochrome P-450 contents was higher in 12 weeks rats than those of in 5 week-old rats, but the increasing rate of that was lower in 12 week-old rats. Hepatic alcohol dehydrogenase activity was also higher in 12 week-old rats than in 5 week-old rats whereas the increasing rate of that was higher in 5 week-old than those in 12 week-old rats by the xylene treatment. Furthermore, the hepatic aldehyde dehydrogenase activities were no differences between the 5 and 12 week-old rats both in the control and xylene-treated group, In conclusion, age may influences upon the hepatotoxicity with xylene and it may be responsible for xylene metabolism in rats.

과학적 지식과의 '관계'속에서 파악되는 이야기적 지식의 의미

한혜정 한국교육과정학회 2007 교육과정연구 Vol.25 No.1

영아기 자녀를 양육하는 어머니와 조모의 상호작용활동

장혜자,정영숙 충북대학교 교육 ·생활연구소 생활과학연구센터 2002 생활과학연구논총 Vol.5 No.-

The purpose of this study is to consider the interaction between two generations carrying on actual bring-up children through investigation of its interaction between mother and grandmother caring their infant. The subjects were 60 pairs of mother as a housewife who has the first child under 24 monthes and grandmother on mother's side or father's side using the inquiry paper method and interview. To take answer, it was distributed/explained directly to each person and then filled out the inquiry. Total 120 sheets were used for final analysis. The measuring instruments were interaction between grandmother and mother according to delivery before and after, it was used the modified 4 questions from barbara J. Myers and Margaret G. Williams petersen(1991)'s study through Grandmother-Mother-Infant Interaction (Jarvos & Creaesy, 1987). For analyzing this matter, it was generated the frequency, percentage, average and value of standard deviation through SPSSWIN program. The results of analyses for this study are as follows. The interaction between grandmother and mother was shown that father-side grandmother often interacted more than mother-side grandmother. It was shown that while mother-side grandmother interacted with mother 'everyday', mother-side grandmother interacted with mother just 2 to 3 times per week, or 1 to 2 times per month.

애기마름(Trapa pseudoincisa S. et. Z.)으로부터 sterol 화합물의 분리 및 구조동정

양혜정,송명종,노영덕,김대근,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

애기마름을 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 H_(2)O로 용매 분획 하였다. EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복 실시하여 2종의 화합물을 분리하였다. 각 화합물의 화학 구조는 NMR, MS 및 IR 등의 스펙트럼을 데이터를 해석하여, stigmasterol과 stigmasterol 3-O-β-D-glucopyranosyl로 구조 동정하였다. 이화합물은 애기마름에서는 처음 분리되었다. Trapa pseudoincisa was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. Two compounds were isolated from the EtOAc fraction through repeated silica gel and ODS column chromatographies. From the result of spectral data including NMR, MS, IR and GC/MS the chemical structures of the compounds were determined as stigmasterol (compound Ⅰ), and stigmasterol 3-O-β-D-glucopyranosyl (compound Ⅱ). They were isolated from Trapa pseudoincisa for the first time.

피칸(Carya illinoensis C. KocH)으로부터 지질대사물질의 분리 및 동정

송명종,한민우,양혜정,이대영,노영덕,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

피칸(Carya illinoensis C. Koch) 열매를 80% MeOH로 추출하고, 추출물을 극성에 따라 n-hexane, CHCl_(3), EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc분획으로부터 silica gel column chromatography를 반복하여 4종의 지질화합물을 분리하였다. 분리된 화합물 화학구조는, NMR 및 MS 등의 스펙트럼을 해석하여 oleic acid methyl ester, linoleic acid methyl ester, β-sitosterol, daucosterol(3-O-β-D-glucopyaranosyl β-sitosterol)으로 동정하였다. Carya illinoensis C. Koch was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with n-hexane, CHCl_(3), EtOAc, n-BuOH and H_(2)O, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as oleic aicd methyl ester(1), linoleic aicd methyl ester(2), β-sitosterol(3), and daucosterol(4). They were the first to be isolated from C. illinoensis.

에어로빅스가 중년여성들의 신체와 정신건강에 미치는 영향 조사

조근종,추혜연 漢陽大學校 體育科學硏究所 2003 體育科學 Vol.23 No.23

The purpose of the study was to investigate of aerobic's effects on body and mental health of middle-aged women. To accomplish of this study, 100middle-aged women from aerobic's training facilities were surveyed by means of the received questionnaires. The questionnaire utilizes a five-point Likert-type scale was employed for ascertaining effects of aerobic's on body and mental heath of middle-aged Women. The use statistical methods used for the data analysis were frequency analysis, T-test. The major findings obtained from this study were as follows: 1. It is displayed that the participation of motivation frequency of the Aerobic's is for the less-weighting, the body maintenance and the solution of stress. 2. It is displayed that the frequency of the solution of stress is solved much stress after Aerobic's. 3. It is displayed that the frequency of the weight changing is to get rid of surplus fat and to get rid of weight after Aerobic's. 4. It is displayed that the frequency for the supporting degree to care the disease is the constipation and the obese. 5. It is displayed that the participant is healthier in mental and body situation. than non participant after Aerobic's. As the results, aerobic dance participation has proven to be an effective form middle-aged women body and mental health.

아동의 역량에 대한 자기지각 : 관련변인의 고찰

한종혜 카톨릭대학교 생활과학연구소 1992 생활과학연구논집 Vol.12 No.1

흰쥐에 사염화탄소 투여시 간 Xanthine Oxidase 활성에 미치는 Allopurinol의 영향

윤종국,이혜자,이상일 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1995 Journal of biomedical laboratory sciences Vol.1 No.1

사염화탄소에의한 간손상시 CCl₄대사에 xanthine oxidase(XO)가 관련되는지를 규명하기 위한 일환으로 allopurinol을 흰쥐 체중 Kg당 50mg을 전처치한다음 CCl₄를 투여한후 처치하여 다음과 같은 결과를 얻었다. CCl₄투여로 인한 간조직의 postmitochondria 분획의 XO활성은 allopurinol을 전처치하므로서 현저히 감소되었으나 투석한 겨울에는 오히려 증가되었으며 type D로부터 type O로의 전환율은 감소되었다. 또한, 투석한 간조직의 XO를 반응속도적인 측면에서 관찰해볼 때 allopurinol을 전처치후 CCl₄투여군이 CCl₄단독투여군보다 Vmax가 크게 나타났다. CCl₄투여로 인한 체중당 간무게의 증가율과 혈청 alanine aminotransferase활성증가율은 allopurinol을 전처치하므로서 저하되었다. 한편 CCl₄투여로인한 간조직중 aniline hydroxylase 및 glucose 6 phosphatase활성감소율은 allopurinol을 전처치하므로서 저하되었다. 이상의 실험결과를 종합하여 볼 때 실험동물에 CCl₄와 allopurinol을 병행투여시 allopurinol이 사염화탄소에의한 간손상을 억제시키는 현상은 XO와 사염화탄소대사간에 관련성이 있음을 시사해주고 있다. To evaluate an effect of xanthine oxidase(XO) reaction system on the carbon tetrachloride(CCl₄) metabolism, CCl₄was given twice at O.1ml/10g body wt. at intervals of 18 hour to the mts and those pretreated with allopurinol (50mg/kg. body wt.). The influence of XO on the metabolism of CCl₄ was focused on the degree of liver damage and the activities of a CCl₄metabolizing marker enzyme, glucose-6-phosphatase. The increasing rate of liver weight per body weight and the levels of serum alanine aminotransferase to the control group were more decreased in allopurinol-pretreated rats than in those treated with CCl₄ alone. The liver XO activities were more increased in CC1₄-treated rats than the control group and the CC1₄-treated rats pretreated with allopurinol showed a decreased activities of XO compared to the CC1₄-treated rats. The type conversion (type D --> type O) rate was more decreased tendency in allopurinol pretreated rats than those treated CCl₄alone. In dialyzed liver enzyme preparations, all of the xanthine oxidase activities: CC1₄-treated, allopurinol and CC1₄-treated rats pretreated with allopurinol showed the more increased Vmax value than the control group, but similar Km value. Moreover, CC1₄-treated rats pretreated with allopurinol showed the more increased Vmax value than the group treated with CCl₄alone. In conclusion, it can not be negate the possibility of metabolism of CCl₄by the xanthine oxidase enzyme system.