냉간성형강 벽체패널의 연결부실험

이영기,Lee, Young-Ki 대한토목학회 2014 대한토목학회논문집 Vol.34 No.3

본 연구의 대상은 석고보드와 냉간성형강 스터드로 구성된 벽체용 합성패널이다. 양면의 석고보드 사이에 위치한 스터드는 나사로 연결되어 있고, 이와 같은 연결부의 부분합성거동을 정량화하여 나사접합부의 단위미끄럼을 유발하는 단위길이 당 전단하중($S_{slip}$)을 산정하는 것이 목적이었다. 실험방법은 1962 AISI규준을 기초로 하여 실시하였다. 본 연구에서 산정한 $S_{slip}$값을 이용한 이론적 추정치와 기 발표된 공칭축강도의 실험값과의 비교에서 만족할 만한 결과를 도출하였다. 그러므로 본 연구의 결과는 연구범위와 유사한 냉간성형강 부재의 해석과 설계에 있어 구조 기술자에게 가치가 있는 정보를 제공할 것이다. The objective of this test series was to determine shear load per unit length which causes a unit slip in the fastener joint. The shear load is one of major factors which reflect partial composite action for cold-formed steel wall stud panels. Test method used were based on the methods presented in the 1962 AISI Specification. According to the comparison with experimental strength, it is seen that the shear loads used in nominal axial strength predictions made acceptable results.

SrAl₂O₄:Eu⁺², Dy⁺³ 장잔광 형광체 합성에 있어서 알루미늄 화합물에 따른 열적거동 및 발광특성 변화

이영기,이유기,Lee, Young-Ki,Lee, You-Kee 한국재료학회 2007 한국재료학회지 Vol.17 No.11

Both photoluminescence and thermal characteristics for $SrAl_2O_4:Eu^{+2},\;Dy^{+3}$ phosphors synthesized with various aluminum compounds (${\alpha}-Al_2O_3$, ${\gamma}-Al_2O_3$, amorphous-$Al_2O_3$ and $Al(OH)_3)$ were investigated in this study. The formation temperature of the host $SrAl_2O_4$ crystal is changed by these various aluminum compounds, as a result of the different thermal decomposition temperature of $SrCO_3$ phase. Among these compounds, the amorphous-$Al_2O_3$ phase shows the lowest formation temperature of the host $SrAl_2O_4$ crystal. The PL emission and excitation spectra of $SrAl_2O_4:Eu^{+2},\;Dy^{+3}$ phosphor are not affected by these aluminum compounds. After the removal of the Xenon lamp excitation (360 nm), however, the excellent longphosphorescent property of the phosphor is obtained by the amorphous-$Al_2O_3$ phase, although the decay time for all phosphors decrease exponentially.

${\beta}-Galactosidase$ 생산 유산균 선별 및 특성 조사

이영기,최수산나,박용일,박찬선,윤병대,황윤식,김희식,Lee, Young-Ki,Choi, Susanna,Park, Young-Il,Park, Chan-Sun,Yoon, Byung-Dae,Hwang, Yun-Sik,Kim, Hee-Sik 한국미생물학회 2006 미생물학회지 Vol.42 No.3

[ ${\beta}-Galactosidase$ ] (lactase)를 생산하는 유산균을 선별하고 그들이 생산하는 ${\beta}-galactosidase$의 특성을 조사하였다. X-gal이 포함된 MRS배지에서 푸른색을 나타내는 약 100여개의 콜로니를 김치로부터 분리하였다. 그들 중 ET-1과 LA-12 두 균주를 최종 선별하였으며, 16S rDNa 염기서열 분석을 통해 각 각 Lactobacillus fermentum과 L. acidophilus와 상동성이 높은 Lactobacillus 속으로 동정되었다. 선별된 두 균주는 높은 ${\beta}-galactosidase$ activeity와 우수한 생존력을 나타내었으며, 이들이 생산하는 ${\beta}-galactosidase$의 최적 활성조건을 조사한 결과, 반응온도 $55^{\circ}C$에서 가장 높은 활성을 나타내었으며, 반응 pH의 경우 ET-1은 pH 5.5에서 최고 활성을 나타내었고, LA-12는 pH7.0에서 가장 높은 활성을 보였다. 선별 유산균 ET-1과 LA-12는 위액과 담즙산액에 대해 우수한 내성을 나타내었다. 두 개의 선별 균주는 인공위액에 3시간 배양 후 초기 생균수와 비교하여 생균수 변화가 거의 없었고, 담즙산액으로 사용한 0.3% oxgall에서 24시간 반응 후에도 1 log cycle 정도 감소된 $10^8CFU/ml$의 생균수를 유지하였다. 이러한 결과를 바탕으로 유산균 ET-1과 LA-12는 유제품 산업에 이용 가능성이 크다고 판단된다. This study was carried out to select the lactic acid bacteria producing ${\beta}-galactosidase$ (lactase) and investigate the properties of the ${\beta}-galactosidase$. About 100 strains of lactic acid bacteria showing blue colony on the MRS agar medium containing X-gal were isolated from several kinds of Kimchi. Among them, 2 strains were selected as potential ${\beta}-galactosidase$ producers. The selected strains, ET-1 and LA-12, were identified as Lactobacillus fermentum and L. acidophilus, respectively by the analysis of 16S rDNA sequences. They showed relatively high ${\beta}-galactosidase$ activity and cellular viability. Their ${\beta}-galactosidase$ showed the highest activity at $55^{\circ}C$. And the optimum pHs of the enzymes produced by ET-1 and LA-12 were pH 5.5 and pH 7.0, respectively. They were also highly resistant to artificial gastric juice and bile. Two selected strains showed little change of viable cell number for 3 hr incubation in artificial gastric juice, and maintained the viable cell number at $10^8CFU/ml$ for 24 hr in 0.3% oxgall after incubation for 2 hours in artificial gastric juice. Based on these results, ET-1 and LA-12 are expected to be applied in dairy industry.

상호확산법에 의한 (Ti , Al)N계 복합질화물의 합성

이영기,김정열,김동건,손용운 ( Young Ki Lee,Jung Yeul Kim,Dong Kun Kim,Yong Un Sohn ) 한국열처리공학회 1997 熱處理工學會誌 Vol.10 No.2

TiN and AlN are ceramic materials with extensive applications due to its excellent mechanical and chemical properties at elevated temperature. The purpose of this research is to develop the method for the synthesis of ternary nitride powder, titanium-aluminum-nitrogen system, which have an excellent property of both TiN and AlN. The ternary nitride such as Ti₃AlN, Ti₂AlN and Ti₃Al₂N₂can be synthesized by the interdiffusion nitriding method in Ar gas, however, the ternary nitride coexist with TiN, AlN, Ti₃Al and α-Ti. The ternary nitride are stable below 1400℃ but these are gradually decomposed into TiN, Ti₃Al and AlN above 1400℃. The thermal oxidation characteristics of the Ti-Al-N compound synthesized by the interdiffusion nitriding method is superior to that of the TiN+AlN mixed powder, and the oxidation for both materials show the differential behaviors.

금속티타늄분말의 질화반응과 산화반응에 관한 연구

이영기,손용운,조영수,김용석,김석윤 ( Young Ki Lee,Yong Un Sohn,Young Soo Cho,Yong Seog Kim,Suk Yoon Kim ) 한국열처리공학회 1995 熱處理工學會誌 Vol.8 No.2

The nitridation kinetics of titanium powder were studied by isothermal and non-isothermal (dynamic) methods in high purity nitrogen under 1 atm pressure. For the comparison with nitridation, the oxidation kinetics of titanium powder were also studied in dry oxygen at 1 atm pressure. An automatic recording electrobalance was used to measure the weight gain as a function of time and temperature. For the reaction with nitrogen, the nitride was formed at over 700℃. The reaction with nitrogen followed the parabolic rate law, and the activation energy was calculated to be 31 ㎉/㏖ in the isothermal method (above 900℃). The non-stoichiometric TiNx has been synthesized by the nitridation at a proper temperature and time, followed by the homogenizing treatment above 1100℃. In comparison with the stoichiometric TiN_(1.0) and the non-stoichiometric TiNx(TiN_(0.5) and TiN_(0.65)), the hot oxidation characteristics of the former is superior to that of the latter. However, both non-stoichiometric nitrides make little difference in the hot oxidation characteristics.

흰쥐 자궁에서 스테로이드호르몬에 의한 c-Fos, CREB, ATF 및 HSP70의 발현에 관한 연구

이영기,김성례,Lee, Young-Ki,Kim, Sung-Rye 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

Steroid hormone is known to cause the dynamic changes of mammalian uterus during reproductive cycle. However there is little information about the effect of estrogen (E) and progesterone (P) on the expression of various transcription factors involved in gene expression. Thus the present study was designed to demonstrate E and/or P-induced expression of c-Fos, CREB, ATF and HSP70 in rat uterus. Rats, ovariectomized (OVX) for two weeks, were divided into 6 experimental groups, 1) OVX, 2) OVX+V, 3) OVX+E, 4) OVX+P, 5) OVX+E+V, 6) OVX+E+P, and western blotting assay for nuclear extract and immunohistochemical staining were carried out for each experimental group. Treatment of E $(10{\mu}g)$ showed to increase the expression of c-Fos, CREB, ATF, and HSP70, and maximal expression was occured at $3\sim6$ hr after E administration. P (1mg) also increased, but much less than E, the expression of c-Fos, ATF, and HSP70. However, P did not reveal any effect on the expression CREE. P treatment 4 hr after E injection decreased c-Fos, CREB, and ATF expression, but did not show any change in the E-induced HSP70 expression. In immunohistochemical study c-Fos-, CREB-, and ATF-immunoreactivities were confined to the cells of luminal epithelium of uterine endometrium. These results suggest that proliferation and differentiation of rat uterus during reproductive cycle may mediated via expression of transcription factors, such as c-Fos, CREB, ATF, and HSP70.

Temporal Changes of c-fos, c-jun, and Heat Shock Protein 25 mRNA in Rat Uterus following Estradiol Treatment

이영기,김성례,Lee, Young-Ki,Kim, Sung-Rye The Korean Society for Reproductive Medicine 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

포유류의 자궁조직은 발정주기를 통하여 역동적으로 변화하고 있으며 이러한 자궁조직의 분화는 시상하부-뇌하수체-생식소를 잇는 축에 의해 조절되는 스테로이드 호르몬에 의해 이루어진다. 그러나 에스트로겐 (E)이 어떤 유전자를 발현하여 자궁 내의 변화를 일으키는지는 아직 자세히 알려지지 않고 있다. 본 연구는 난소를 절제한 성숙한 흰쥐에 E을 처리한 후 자궁조직 내에서 c-fos, c-jun 및 hsp25 mRNA의 발현 변화를 Northern blot analysis방법을 사용하여 연구한 것이다. c-fos및 c-jun 암원유전자의 mRNA발현은 E처리 후 1시간 이전부터 증가하기 시작하며, 3시간 이내에 최고치에 도달한 후 급격히 감소하여 기저수준으로 되돌아갔다. 반면에 hsp25 mRNA수준은 E처리 후 3시간 대에서 최고치를 나타내나 증가된 발현량이 서서히 감소하며 12시간이 지난 후 까지도 정상대조군에 비해 높은 수준으로 유지되었다. 이러한 E의 영향이 선별적인지를 검증하기 위하여 E의 길항제인 tamoxifen을 사전처리하고 E을 추가로 처리하여 c-fos, c-jun및 hsp25 mRNA의 발현이 최고치에 이르렀던 3시간대에 자궁조직을 얻어 각각의 유전자 발현량을 조사한 결과 E에 의해 증가되었던 c-fos, c-jun 및 hsp25 mRNA의 수준이 억제됨을 확인하였다. 이러한 결과는 E이 자궁조직에 영향을 미치는데 초기의 일시적인 변화를 보이는 암원유전자인 c-fos 및 c-jun이 중요한 역할을 하리라는 것을 시사하며 hsp25의 경우는 좀더 늦은 반응에 관여하거나 c-fos및 c-jun에 의하여 간접적으로 조절을 받을 수도 있음을 보여 주는 것으로 사료된다. Steroid hormone is known to cause the dynamic changes of mammalian uterus during reproductive cycle, which are modulated via hypothalamus-pituitary -gonad reproductive endocrine axis. Although there were so many studies about estrogenic regulation of uterine growth and differentiation. There is little information about the effect of estrogen on the expression of various transcription factors involved in gene expression. Thus the present study was designed to demonstrate E induced expression of c-fos, c-jun, hsp25 mRNA in rat uterus. Employing Northern blot analysis, we studied the temporal expressions of c-fos, c-jun, and hsp25 messenger RNAs (mRNAs) elicited by a single 17beta-estradiol (E) treatment in the uteri of bilaterally ovariectomized adult rats. c-fos, c-jun, and hsp25 mRNA levels were increased and peaked at 3h after E administration, and then c-fos and c-jun mRNA levels were rapidly decreased to basal control level while, increased hsp25 mRNA levels were sustained till 12h post E treatment. To test the estrogenic effect on the increase of c-fos, c-jun, and hsp25 mRNA levels, we also examined the effects of antiestrogen (tamoxifen). Pretreatment with tamoxifen effectively blocked the E-induced increase of c-fos, c-jun, and hsp25 mRNA levels at 3h post E treatment. Present results suggest that transient increase of c-fos and c-jun protooncogene mRNA at the early time and simultaneous expression of hsp25 mRNA contribute to the response of uterine tissues to E in adult female rats.

Eu 부활형 Sr-Al-O 계 장잔광 형광체의 합성과 잔광특성

이영기,김정열,김병규,유연태,Lee, Young-Ki,Kim, Jung-Yeul,Kim, Byung-Kyu,Yu, Yeon-Tae 한국재료학회 1998 한국재료학회지 Vol.8 No.8

$SrAI_2O_4:Eu^{2+}$를 합성하여 장잔광 축광재료로서 가장 중요한 발광특성과 장잔광특성을 조사하였다. 융제로서 $B_2O_3$를 3wt% 첨가한 $SrCO_3$, $Eu_2O_3$, $AI_2O_34의 혼합분말은 $1000^{\circ}C$이상에서 모체결정인 $SrAl_2O_4$의 단일상이 형성되었으나, 장잔관 형광체로서 최적의 합성조건은 $1300^{\circ}C$, 3시간이었다. 그리고 $SrAI_2O_4:Eu^{2+}$는 부활제인 $Eu^{2+}$의 $4f^65d^1$ $\rightarrow$$4f^7$천이에 기인하여 황록색발광영역의 520nm(2.384eV)를 최대 발파장으로 하는 450~650nm의 폭넓은 발광스펙트럼을 보였으며, 또한 발광파장을 520nm로 고정하여 측정한 여기스펙트럼의 최대 흡수피크는 360nm이고 250nm에서 480nm의 넓은 범위에서 흡수스펙트럼이 관찰되었다. The synthesis of $SrAI_2O_4:Eu^{2+}$ phosphor and its properties of both photoluminescence and long-phosphorescent were investigated as a function of sintering condition. Single phase of $SrAl_2O_4$ was obtained by sintering the mixtures of $SrCO_3$, $Eu_2O_3$, $AI_2O_34 and 3wt% $B_2O_3$ powders over 100$0^{\circ}C$ in Ar/H2 atmosphere. The optimum sintering condition for the long-phosphorescent phosphor of $SrAI_2O_4:Eu^{2+}$ was found at 130$0^{\circ}C$ for 3hours. The PL emission spectrum of $SrAI_2O_4:Eu^{2+}$ shows a maximum peak intensity at 520nm(2.384eV) with a broad emission extending from 450 to 650nm which resulted from the $4f^65d^1$$\rightarrow$$4f^7$ transition of $Eu^{+2}$ under 360nm exitation. Monitored at 520nm. the excita￢tion spectrum of $SrAI_2O_4:Eu^{2+}$ exhibits a maximum peak intensity at 360nm (3.44eV) with a broad absorption band extending from 250 to 480nm.

(Ti1-xAlx)N 계 질화물의 소결특성에 미치는 Co , CO - Ti 금속결합제의 영향

이영기,손용운 ( Young Ki Lee,Young Un Sohn ) 한국열처리공학회 1998 熱處理工學會誌 Vol.11 No.3

N/A The purpose of this research is to investigate the effects of Co, Co-Ti addition on the sintering characteristic of (Ti_(1-x)Alx)N material synthesized by the direct nitriding method for a application as a cermet material. The observed shrinkage rates of (Ti_(1-x)Alx)N pellets increase with the additive (Co, Co-Ti) content, temperature and time, and also the pellets with the same additive content exhibit the shrinkage behavior that depends on the Ti/Al ratio. However, although the shrinkage rates in this study is the mast higher (36%), the density of the sintered (Ti_(1-x)Alx)N pellet is below 80% density in theory because of the partial segregation and the dense band defect of AlCo compound. Consequentely, it is considered that Co was not effective as a binder material because the wettability of liquid Co metal on (Ti_(1-x)Alx)N materials is poor, In (Ti_(1-x)Alx)N with Ti-Co additive, the stoichiometric TiN is transformed by the under-stoichiometric TiNx(x<1.0) during sintering, leading to the good properties such as hardnees and hot oxidation.

술폰화된 , 저분자량 폴리스티렌 이오노머를 이용한 새로운 형태의 단이온 전도체

이영기,박정기 ( Young Gi Lee,Jung Ki Park ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.2