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상추 유전자원의 원산지, 형태 및 엽색별 Bitter Sesquiterpene Lactones의 변이
최수산나,아와리스 아세파,이재은,성정숙,허온숙,노나영,이석영,이호선,이주희 한국국제농업개발학회 2018 韓國國際農業開發學會誌 Vol.30 No.3
Lettuce (Lactuca sativa L.) is one of the most important leafy vegetables in the world due to its health related benefits. Bitter sesquiterpene lactones (BSLs) are crucial secondary metabolites of lettuce which are useful in many physiological activities, and also has been used to relieve pain. This study was conducted to appraise the variability of phenotypic characteristics and the total content of BSLs in fifty lettuce germplasm. The germplasm collections exhibited wide variation in both qualitative and quantitative characters. The average plant weight was 333.3 g with a range from 60.0 to 700.0 g and the lactucin content was varied from 3.4 (IT265031) to 448.6 (IT300287) μg/g DW with an average concentration of 94.0 μg/g DW. Whereas lactucopicrin ranged from 6.8 (IT217859) to 2,714.9 (Superseonpung) μg/g DW with an average concentration of 805.8 μg/g DW. Total BSLs content was ranged from 19.6 (IT217859) to 2,821.9 (Superseonpung) μg/g DW with the average concentration of 899.8 μg/g DW. Significant (p≤0.05) differences in lactucin, lactucopicrin and total BSLs content were found among the germplasm collections. Leafy type lettuce germplasm collections originated from South Korea revealed the highest average total BSLs content (2,821.9 μg/g DW). Principal component analyses of lactucin and lactucopicrin generated based on leaf color and plant growth type yielded two principal components, PC1 and PC2, which accounted for 60.8 and 39.2 % of the total variance, respectively. Our study provides fundamental insights on phenotypic characteristics and the total content of lactucin and lactucopicrin of lettuce germplasm which may further help researchers and breeders to produce improved lettuce varieties.
배추 유전자원의 형태 특성 및 β-Carotene과 Canthaxanthin 변이
최수산나,Awraris Derbie Assefa,이재은,성정숙,허온숙,노나영,이호선,이석영,이주희 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Carotenoids are pigments that are found in plants, fruits, bacteria, and fungi. β -carotene and canthaxanthin are orange pigments among thousands of carotenoids that possess potent antioxidant activity. The objective of this study was to determine β-carotene and cantaxanthin in 55 Kimchi cabbage germplasm using liquid chromatography mass spectrometry (LC-MS) method. Method and Results : 55 Kimchi cabbage accessions were sown in plug trays in the end of August, 2016. All the tissue samples were freeze-dried for 1 week and ground into fine powder and stored at -20℃ until analysis. Crude carotenoids from freeze-dried materials (100 mg) were extracted using 1 ㎖ of 100% (v/v) hexane in 10 minutes by using sonication bath followed by centrifugation. The average β-carotene contents was 1.43 ㎎·㎏-1 and ranged from 0.76 (IT 120045) to 2.25 ㎎·㎏-1 (IT 100378). The average canthaxanthin content in the entire domain of sample was 0.59 ㎎·㎏-1 with a range between 0.36 (IT 32746) and 1.08 ㎎․㎏-1 (IT 100386). Canthaxanthin content was significantly positively correlated with β-carotene (r = 0.65**) and leaf length (r = 0.63**). However, canthaxanthin was negatively correlated with cotyledon color (r = -0.41**). Principal component analysis results of the first two components (PC1 and PC2) explained 44.53% of the point variability. Conclusion : The resources with the highest β-carotene and canthaxanthin content are IT 100378, 100386, 100391, and 110828. This study could be useful to select a potential sources of health beneficial carotenoids (β-carotene and canthaxanthin) in Kimchi cabbage germplasm in nutraceutical formulations and for further applications as a breeding material and other research activities.
hERG 칼륨 채널 매개성 HEK 293 세포를 이용한 한약 처방의 심장 독성 평가 연구
최수산나,권오빈,이시온,서창섭,신현규 대한한의학회 2019 대한한의학회지 Vol.40 No.2
Objectives: The correlation between medicinal herbal formulas and a risk of cardiotoxicity has been controversial. Thus, this study investigated cardiac safety assessment of 52 most commonly used medicinal herbal formulas. Methods: We evaluated the in vitro effects of medicinal herbal formulas on recombinant human embryonic kidney 293 (HEK 293) cell line expressing human ether-a-go-go-related gene (hERG) potassium channel. Results and Conclusion: Our results demonstrated that all of 52 medicinal herbal formulas did not show inhibition of hERG current in hERG-HEK 293 recombinant cells. In conclusion, these safety data suggest that 52 medicinal herbal formulas were not associated with an increased risk of cardiotoxicity in hERG-HEK 293 recombinant cells.