난소절제후 Estrogen을 투여한 흰쥐 대동맥에서 Protein Kinase C-α와 Fas 발현의 변화

이왕수,이상엽,안지현,송영빈,김학진,이광재,유재격,김상욱,김태호,김치정,류왕성 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.3

Estrogen is believed to decrease coronary artery disease. Protein kinase C-α(PKCα)appear to be important in signal conduction pathways. Estrogen treatment increases catalytic activity of PKCα,and activation of PKCαcan modulate estrogen receptor levels and responsiveness. And it was reported that activation of PKC can protect cells from apoptosis induced by Fas ligation. But the beneficial effect of estrogen on PKCαwas not clearly demonstrated. The objective of this study was to evaluate the role of PKCαafter ovariectomy and estrogen therapy in rat aorta. The results were summarized as below: 1. The significant pathological changes were not observed in the rat aorta irrelevant to ovariectomy and estrogen therapy. 2. The expression of Fas was decreased in the aorta of estrogen-treated rats than that of ovariectomized rats without estrogen therapy. 3. Increased expression of PKCαwas more marked in the aprta of estrogen-treated rats than that of ovariectomized rat without estrogen therapy. In conclusion,PKCαmay be important in signal conduation pathway on the effect of estrogen. Activation of PKCαby estrogen reduced Fas expression, suggesting that PKCαactivation may play a role in protection against atherasclerosis. Further studies are needed to elucidate the role of PKCαafter estrogen therapy.

Bleomycin 투여가 mice신장에 미치는 독성에 관한 생리학적, 생화학적 및 초미세형태학적 연구

김상엽,조성태 광주보건대학 1991 論文集 Vol.16 No.-

Bleomycin, a glycopeptide antibiotic isolated from Streptomyces Verticillus, ha been used as an anticancer drug active aganist human squamous cell carcinoma of skin and lung. In this experiment, the author pursed the effect of bleomycin on the mouse kidney, observing the physiological, biochemical and ultrastructural changes in glomerulus and convoluted tubules. Mice(ICR strain) were used as experiment animals which were divided into two groups, the control and experimental groups. In order to elucidate the nephrotoxicity of the mouse administrated bleomycin in D. W., this study was carried out. Bleomycin was injected 20 units per kg of body weight to abdominal portion of mouse in 1 week, 2 weeks, 3 weeks, and 4 weeks. After 5 weeks, the left kindey was taken out from the mouse with injicted bleomycin and investigatigated the damages of the cells by the ultrastructural inspections. The results obtained were as follows 1. The thickened basement mimbrane of glomerulus and closed urinary space, fused parietal epithelial cells were observed, respectively. 2. Destructed brush border, granular degeneration, formed crescent, fused foot process, vacuolation of proximal and distal convoluted tubules were shown. 3. Phsiological characteristics of experimental groups, thrombocyte, Hematocrit and MCV were significantly increased. 4. Total lipid concentration, blood urea nitrogen were slightly increased and total cholesterol and albumins were significantly increased on all experimental groups. 5. In the structural changes of the mitochondria in kidney treated with bleomycin the organelles changes into a spherical or oval shapes, irregular cristae and destructed mitochondria were shown. From the above results, bleomycin damages both proximal and distal convoluted tubular epithelial cells and glomerulus in mice kidney.

E.coli의 pBN18 플라스미드 DNA의 구조 및 발현의 전자현미경 관찰

김상엽 광주보건대학 1994 論文集 Vol.19 No.-

This study was visualized the transcriptional activity, the size and the modelof plasmid DNA by electron microscopy. Plasmid pBN18, pBN18A and pBR322 were visualized by way of electron microscopy. Mica-press adsorption method that alows selective visualization of the plasmid DNA released in situ from the bacterial cell is rapid and useful for visualization of plasmids. The released plasmid DNA was adsorbed preferential on mica in a divalent cation-free solution, and the Miller chromatin-spreading method may allow the physical interaction and transcription to be visualized. Therefore mica-press adsorption method and Miller chromatin-spreading method used to visualize the plasmid DNA, chromosomal DNA and gene expression. As a result, their estimated sizes of plasmid pBN18, pBN18A, pBN18B and pBR322 were 1.87 0.28(5.6 Kb)?, 1.53 0.12(4.6Kb)?, and 1.50 0.03(4.50Kb)?, respectively, and transcriptional activity was observed by the visualization of gene expression of chromosomal DNA.

견부통 환자의 임상적 고찰

김상엽 광주보건대학 1995 論文集 Vol.20 No.-

The subjects of this study were 104persons who received physical treatment for shoulder pain of Dong Jin Clinic Center during the period Januray 1, 1995 to April 30, 1995. Analysis of patient's general characteristics were carried out. The subjects were 38(36.5%) males and 66(63.5%) females. their average was 46.7 years with the largest group of 40-59 years (31persons or 29.8%). The majority of patients came for physical treatment in 3months after onset of shoulder pain(66persons or 63.5%). The patients who have complained shoulder pain and limitation of motion were 52(50.0%)persons, posterior neck pain and limitation of motion were 45(43.3%)persons. The patients diagnosed as frozed shoulder were 48(46.2%)persons, sprain were 26(25.0%)persons. As for the average duration of the physical treatment was 9.7days with the largest group within 5days (42persons or 40.4%).

光電 方法에 의한 MOS구조의 산화층내의 電荷分布 연구

심성엽,金末文,이동건,문영희 嶺南大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.13 No.-

Charge trapping in the insulator of a metal-oxide-semiconductor (MOS) structure strongly influences photocurrents resulting from electron photoinjection from gate into this insulator. These electric characteristics are investigated by Przewlocki method for determination of trapped charge distribution in the dielectric of a MOS structure. Silicon dioxide films were grown thermally in a dry oxygen ambient at 1000℃ to a thickness of 2000Å on (100) 6-9Ωcm p-type Si Wafers. As a result, it is found that the negative charge distribution is an exponential profile of decay lengthλ=9.907×10??cm and trapped charge density N。=3.912×10??cm-₃in the dielectric of this MOS structure.

Pentoxifylline과 고압산소요법이 백서 무축 피판 생존율에 미치는 영향

김사엽,이상한 慶北大學校 齒科大學 1991 慶北齒大論文集 Vol.8 No.2

The effects of pentoxifylline, hyperbaric oxygen and combination of the two on the survival rate of random pattern skin flaps were evaluated in an experimental study in rats. Thirty-five rate were divided into five groups. Cephalic based random pattern flaps were designed on the back of the rats and control group animals received twice-daily an intraperitoneal injection of 1cc normal saline solution for seven days before surgery and every 12 hours after the operation for seven days and to control for the stress of injection. Group I rats were treated with pentoxifylline received 20 ㎎/kg intraperitoneally at 24, 12, and 1 hour(s) before flap elevation and every 12 hours after the operation for seven days. Group Ⅱ rats were treated with twicedaily pentoxifylline injections for seven days before surgery and were then managed as Group I. Group Ⅲ rats were treated with hyperbaric oxygen within four hours after flap operation. Group Ⅳ rats were given exactly as Group Ⅱ and Group Ⅲ. The survival rate to the total flap was calculated. The results were as follows: 1. The mean survival rate of the flaps was 54.60±16.78% in control, 61.34±10.62% in group I, 64.96±3.08% in group Ⅱ, 71.93±9.26% in group Ⅲ, 86.67±8.17% in group Ⅳ. 2. The survival rate of the flaps were increased in group Ⅰ,Ⅱ, Ⅲ, Ⅳ when compared with the control group. 3. The combination of pentoxifylline and hyperbaric oxygen showed synergistic effect on the survival rate of the flap. 4. In the histopathologic study at postoperative 7th day, abundant proliferation of fibroblast and capillary blood vessels were observed in the experimental Ⅲ group, while severe infiltration of inflammatory cells were observed in the control group.

웹서버를 이용한 그룹웨어 시스템의 설계 및 구현

김성연,최용엽 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

이전까지의 결재방식은 직접 결재문서를 들고 결재자로부터 결재를 받는 방식이었으나 이제는 결재자가 집에서 또는 출장 중에 있어서도 웹 상에서 서버에 접속하여 결재자에게 전달되어진 결재문서를 검토, 결재할 수 있도록 하는 그룹웨어시스템이 필요하게 되었다. 본 연구개발의 주된 목적은 사내에서 기존의 결재방식을 웹 상에서 전자결재방식으로 변경하여 문서의 기안작성, 검토 결재 및 결재상황을 실시간으로 파악할 수 있도록 하였으며 또한, 이러한 모든 결재문서들은 데이터베이스에 최근문서, 일반문서, 정형화된 문서로 구분되어 저장되도록 하여서 권한이 부여된 사용자가 필요시 찾고자하는 문서를 실시간으로 검색 될 수 있도록 시스템을 구축하였다.

GC/NPD를 이용한 다성분 잔류농약의 동시분석

김우성,이선화,김상엽,정동윤,김재이,이영자,이홍재,정성욱,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with fiorisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD, Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.

DNA의 분리 및 제한효소에 관한 연구

김상엽,박민희 광주보건대학 1993 論文集 Vol.18 No.-

The pBN18 of the gene fusion vector was double digestion with HindⅢ and EcoRⅠ for the analysis of the restriction enzyme sites. As a results, the fragments of 1.2Kb and 4.6Kb were made. Therefore it was conformed that pBN18 includes the sites of HindⅢ and EcoRⅠ. The pBN18 contained the ClaⅠ site to digestion with ClaⅠ restriction enzyme. A study was conformed the restriction enzyme sites of pBN18, pBN18A and pBN18B and DNA sizes of pBN18 and pBN18B were compared with the agarose gel of the electrophoresis.

不純物에 依한 Barium Titanates의 電氣的 性質

박창엽,박상만,김현재,양재면 연세대학교 산업기술연구소 1975 논문집 Vol.6 No.1

Semiconductive Barium Titanates was prepared and its electrical properties were investigated. For the purpose of controlling in valency Al₂O₃, SiO₂, TiO₂ and Sb₂O₃were doped in Barium Titanates as additions. 0.1∼0.2mole% Sb₂O₃ was effective in reducing resistivity of the order of 10³ohm-cm. Semiconductive Barium Titanates showed anomalous positive character in the temperature dependency of resistivity. It is proposed that conduction in those samples is caused by exchanging electrons between ?? ions and ?? ions.