RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

素問·繆刺論에 對한 硏究(Ⅱ)

柳太植,鄭憲瑩,琴坰樹,朴炅 대한한의진단학회 1998 大韓韓醫診斷學會誌 Vol.2 No.2

The Scripture, the research on The MuJa Ron(○刺論) of the So Moon(素問), is described in the MuJa(○刺) of the Kap U1 Kyung(甲乙經). Also it is written in MuJa of Tae So(太素) volume 23,. And Wang(王○) interpreted it in volume 18 chapter 63. In ChimJaRyu(鍼刺類) of RyuKyung(類經) volume 20, it is introduced. And it is mentioned in the 2nd chapter of ShinKyoJung(新校正). In MuJa(○刺), Mu(#) means crossing left and right. Since this paper is describing the differences between MuJa(○刺) and KeoJa(巨刺), and it is also mentioning about carrying out the idea into practice, it is named MuJa(○刺). This paper, the theory of MuJa(○刺), can be divided into three properties in content. Chapter Ⅰ. deals with the common points and differences comparing with KeoJa(巨刺). Chapter Ⅱ. treats the idea that since RakMaek(絡脈) which is invaded by pathogenic factors are different, the states of the disorder are appeared differently so the place, method, atid number and order of acupuncture should be different. In chapter Ⅲ. it is said that in the state of disease of Kyung(經), KeoJa(巨刺) should be applied and in the state of the disease of Rak(絡), MuJa(○刺) should be used. treating oppositThe metabiosis of pathogenic factors flows from the skin and bodyhair to SonRak(孫絡), SonRak(孫絡) to RakMaek(絡脈), RakMaek(絡脈) to internal organs in general. In this situation KeoJa(巨刺) could be used in general. But if pathogenic factors flows irregularly, overflowing from one RakMaek(絡脈) to another place rather than internal organs, MuJa(○刺) could be applied. Therefore in the acupuncture treatment, the theory of MuJa(○刺) which implies side of the troubled places rather than the site itself is very important. But MuJa Ron(○刺論) is too difficult to interpret since is written in old Chinese, the writer of this paper has researched to prove the exact meaning of the MuJa(○刺).

사람 말초혈액 림프구에서 Bromodeoxyuridine에 의해 유도되는 Fraglie Sites

박경란,김원식 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Chromosomal abnormalities including fragile sites induced by 50㎍/ml and 100㎍/ml bromodeoxyuridine (BrdU) was studied in lymphocytes cultures of six healthy individuals. A significant decrease in mitotic indexes in respect to control cultures was observed with both BrdU concentrations used. The cells showing chromosome aberrations and the total number of cytogenic alterations were significantly increased both BrdU treated cultures with respect to control cultures. Damaged cells showing chromosome aberrations and total number of chromosomal aberrations were increased in BrdU, 100㎍/ml treated groups compared to BrdU 50㎍/ml treated groups. The most frequent type of chromosomal aberration is a gap. A site showing a gap or break was defind as fragile if it appeared in 1% of cells analyzed and in at least three of six individuals studied with the same culture treatment. Using these criteria, fragile sites 4q31, 5q31, 6p22, 6q13, 9q21, and 15q24 were induced in different proportions by different concentration of BrdU.

온쓸개관을 묶은 집토끼 위날문점막의 미세구조 연구. : Ⅰ. 날문부 표면점액세포 Ⅰ. Pyloric Surface Mucous Cell

박경호,오승종,고정식,안의태,박대균,정은주 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The purpose of this study was to examine the ultrastructural characteristic of the normal pylorus mucosa, and their structural changes induced by the ligation of common bile duct of the rabbit. Common bile duct ligation probably causes the dysfunction of the pyloric surface mucous cells that results in delayed mucous formation and secretion, but recovered mucous secretory activity is recovered on the late stages.

한국에서 한 일간지 신문기사 검색을 통한 IMF 전과 후의 자살 양상 비교

박상학,김경환,김학렬,김상훈,표경식,조용래 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Objectives : Under an IMF bailout program, our country's financial situation forced people to change many things suddenly. Therefore, this study was attempted to search the aspects of changes of suicide attempts in order to find out how the financial crisis affects people's suicide attempts, the extreme repertoire for psychological change Method : November 21, 1997, the day issued the application of IMF bailout program, is the basic date for comparing the changes, on a certain newspaper(Dong-A daily newspaper). The condition was divided into two, namely before and after the basic date, and the characteristics of suicide were compared between two conditions. Results : The news of suicide increased more after than before the application of IMF situation. (before:105, after:158) Specially, for 30-50 years old, active age in community, the rate of suicide attempts reached twice than that of the others. After the IMF situation, the prominent reason of suicide was the financial crisis. For the type of suicide, the family suicide was remarkably increased after the IMF situation. Conclusion : The frequency of the news of suicide was higher after than before IMF bailout program. The major reason of suicide was the financial crisis. Considering that the social and economic crisis affects people's view of death, psychosocial interventions are necessary for their mental health.

저열량영양식품 섭취에 따른 비만여성의 체중감량 효과에 관한 연구

박순영,최중명,김동현,최경식,최봉근,윤태영,유동준 한국보건통계학회 2002 보건정보통계학회지 Vol.27 No.1

In the method of obesity treatment, Dietary method, Exercise method, Behavior method, and Drug method, Dietary method is the most important control method of obesity. The aim of this study is that evaluate the weight reduction effect of the specific low calorie diet products (KSD I and 2) in obese women. Twenty five women who were volunteer participated in this study. They have no heart diseases and metabolic diseases. we classified two groups : obese group and standard group. Anthropometric indices were measured in all subject for four times. Venous blood sample were taken to determine the lipid profiles, glucose level, and liver function test. In obese group, body weight were reduced 2.85±1.46kg, but showed statistically non-signicicant. Body mass index were gradually reduced, 1.09±0.93kg/m^2(p<0.05).In blood analyses, total cholesterol were reduced 10.61 ±33.4mg/dℓ ( p<0.05). KSD 2 significantly inhibited maltase, sucrase and α-glucosidase from rat intestine, and porcine pancreatic lipase, although KSD I inhibited only the sucrase.

건축물 3종 안전점검 사례분석에 따른 기법 및 제도 개선에 관한 연구

Park,In Seo,Jeon,Kyung Sik 경기대학교 재난안전연구소 2020 재난안전논문집 Vol.2020 No.1

연구목적 : 본 연구는 건축물 3종 정기안전점검의 현장점검·보고서의 개선을 목적으로 한다. 연구방법 : 연구를 위해 건축물 3종 정기안전점검의 현장점검·보고서 문제점을 진단점검 기술자에게 5점 리커트 척도로 구성하여 79명을 대상으로 설문조사를 실시하였다. 연구결과 : 현장 구조체 외부에 마감재로 시공되어있어 구조체의 확인이 불가한 상태이다. 이로 인하여 점검대상 시설물의 현장조사시 결함여부에 대한 조사가 곤란하여 객관적인 평가에 문제점이 많다. 따라서 최초 설계시에 유지관리을 고려한 마감재선정, 점검구 배치가 바람직하다. 주요구조부에 대한 추가적인 점검구설치, 리모델링공사시 점검구 설치 등 이 필요하다. 복사 붙여넣기식 보고서는 많은 수의 보고서 작성시 유사한 결함과, 기존에 점검시에 결함내용과 동일한 결함조사 내용으로 판단하고 점검기술자가 보고서를 작성하게 되므로 인하여 건물특성에 적합하고, 유지관리에 도움이 되지않는 문제점이 있다. 결론 : 관리주체의 의식교육, 마감재에 의한 구조체 확인한계, 점검기술자의 교육, 저가수주에 의한 기술자 및 점검시간 부족방지를 위해 대가의 현실화, 3종 시설물 평가 Check list의 개선, 시설물 관리주체의 의도에 따른 점검, 복사 붙여넣기식 보고서, 종합결론과 조사내용과 불일치에 대한 개선이 필요한 것으로 나타났다. Purpose: This study aims to improve on-site inspection and report of three-classes buildings. Method: For the purpose of the study, problems with field inspection and report of three-classes buildings were organized into five-point clock scale for the diagnostic inspection engineer and conducted a survey of 79 people. Results: The structure cannot be verified as it is constructed with finishing materials outside the site structure. As a result, there area many problems in objective evaluation as it is difficult to investigate whether a facility is defective during an on-site inspection. Therefore, it is desirable to re-select the finish and deploy the inspection tools considering maintenance at the time of initial design. Additional inspection tools for major structural parts and installation of inspection tools for remodeling work are required. Copying and pasting reports are considered to be similar in the preparation of a large number of reports, and are considered to be the same defect investigation details as the previous inspection results, and the inspection engineer prepares the report, which is suitable for building characteristics of the building and is not conducive to maintenance. Conclusion: It was found that improvement of consciousness education of the managing body, verification of structure by finishing materials, education of inspection engineers, improvement of the checklist of three types of facilities evaluation, inspection of the intention of the managing body of the facility, and comprehensive conclusion and survey contents and inconsistency are needed to prevent shortage of inspection time.

고장으로 인한 대구경 계량기의 불감률

박남식,배정용,황경석,김학용,최철식 대한상하수도학회 2003 상하수도학회지 Vol.17 No.2

Leaks and meter under registrations are suspected as primary components of unaccounted-for water in Korea. Roughly, one third of the unaccounted-for water is believed to be due to meter under registration. However, no justification has been given to prove the statistics. In this study the rate of meter under registration due to failures of large-diameter meters is investigated. Replacement records of over 2600 consumer sites are analyzed. The minimum, the maximum and the probabilistic distribution of meter under registration rate are computed.

설비보전관리와 안전정보를 연계한 Web 상에서 지식베이스 진단시스템 구현

박주식,강경식 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

To keep enterprise's competitiveness on condition of the automatic manufacturing system such as FA, FMS and CIM, all the maintenance problems should be considered seriously in not only production and maintenance but also related industrial safety. As we analyze in the surveys for the maintenance management of domestic enterprises and the causes of industrial accident, there will be necessity of drawing up countermeasures for prevention of industrial accidents and for ensuring expertise maintenance technologies. Based on these analyses, this study studied the safety information system, maintenance management information system, and the machinery condition diagnosis technique by using of the knowledge-based system under the internet environment. This web based knowledge-based diagnosis system can easily provide not only the knowledge of expert about deterioration phenomenon of industrial robot, but also the knowledge of relating safety and facility on everywhere, everytime, Therefore, when we use this system, it is expected to improve the efficiency of business processes in the production and safety.

BCG를 대량 투여 받은 생쥐 가슴샘 겉질의 미세구조

고정식,안의태,박경호,우성임,한영복,경홍기 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.3