Transthyretin의 Glutathione 자동산화 촉진작용

박종근,정신,김재휴,김수한,강삼석,이제혁,안봉환 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.8

When reduced glutathione(GSH) was incubated at neutral pH and at 37°, its concentration decreased slowly with formation of oxidized glutathione(GSSG). Autooxidation of GSH was accelerated by Cu^(2+) and Hg^(2+), but not by other common mono-, di-, and tri-valent cations. Transthyretin was found to stimulate autooxidation of GSH in the presence or absence of Cu^(2+) and Hg^(2+). EDTA inhibited perfectly the autooxidation of GSH regardless of the presence of transthyretin. The stimulating activity of transthyretin was maximal at pH 7.0, declining progressively with increase or decrease of pH from 7.0. Sulfhydryl-blocking agents such as p-hydroxymercuribenzoic acid and Nethylmaleimide markedly inhibited the stimulating activity of transthyretin. Transthyretin stimulated autooxidation of other sulfhydryl compounds such as clithiothreitol and cysteine. However, it did not show a significant effect on autooxidation of sulfhydryl group of egg albumin and eye lens proteins. And transthyretin did not cause any oxidative change to thyroxine(T₄), 3, 5, 3Ltri iodo thyronine(T₃) and 3, 3: 5~triiodothpnine(rT₃) bound to it in the presence of GSH and Cu^(2+). The above results suggest that transthyretin may play a role in regulation of oxidized status of sulfhydryl groups in blood plasma and cerebrospinal fluid

키토올리고당을 첨가한 청국장의 품질개선에 미치는 영향

김제중 ( Je Jung Kim ),정병옥 ( Byung Ok Jung ),정석진 ( Suk Jin Chung ),이선호 ( Sun Ho Lee ),왕태환 ( Tae Whan Wang ),이근보 ( Keun Bo Lee ),박상순 ( Sang Soon Park ) 한국키틴키토산학회 2010 한국키틴키토산학회지 Vol.15 No.2

청국장의 품질을 개선하고자 키토올리고당의 첨가량을 달리하여 청국장을 제조한 후 생균수 변화를 살펴보면 무첨가 구인 C-0의 경우 3.14 × 108 CFU/g으로 나타나 발효가 순조롭게 진행 된 것을 알 수 있었으며, 키토올리고당이 첨가된 C-0.2의 경우 2.95 × 108CFU/g, C-0.4는 2.81 × 108CFU/g 그리고 C-0.6의 경우 1.45 × 108CFU/g 결과로 보아 키토올리 고당의 함량이 0.6%이상에서는 미생물생육에 영향을 주는 것을 확인 할 수 있었다. pH 변화는 무첨가구인 C-0.0의 pH는 7.14를 보이고 있고, C-0.2, C-0.4, C-0.6의 경우 각각의 pH 변화를 살펴보면 7.61~7.67로 특별한 유의차를 발견하지 못했다. 점질물의 변화는 무첨가구인 C-0.0의 10.5%의 함량을 보이고 있으며, C-0.2 9.92%, C-0.4 10.3% 그리고 C-0.6 10.1%의 함량을 나타내고 있다. 이결과로 키토올리고당이 청국장의 점질물 생산에 특별한 영향을 주지 않는 것을 확인할 수 있었다. 반면 항산화 활성의 변화는 무첨가구인 C-0.0에서 67.6%의 항산화 활성을 보이고 있으며, C-0.2 67.7%, C-0.4 72.6% 그리고 C-0.6 78.2%의 항산화 활성을 나타내고 있다. 관능평가의 경우 색상은 C-0.4에서 6.77, 향의 경우 C-0.0에서 5.86 그리고 질감의 경우 C-0.4에서 5.33 가장 높은 값을 나타내고 있고, 선호도는 C-0.4이 6.28 전체적으로 높은 결과를 보여주고 있다. 전체적으로 시료간의 유의차를 보여주고 있지 않지만, C-0.4에서 다른 시료보다 좋은 점수를 얻었고, 항산화 활성, 총산등의 결과로 보아 키토올리고당을 첨가한 경우 청국장의 품질개선에 기여할 것으로 판단된다. In order to improve the quality of fast-fermented bean paste (so called, cheongkuk-jang), we made cheongkuk-jang with chitooligosaccaride and examined viable cell count. We also prepared several kinds of cheongguk-jang with different concentration of chitooligosaccaride. The results showed that the cheongkuk-jang formed exhibited the effective microorganism growth and antioxidative activity. Good results of sensory test are C-0.4 in color, C-0.0 in flavor, and C-0.4 in preference test.

Hepatic Veno-Occlusive Disease Following Allogeneic Bone Marrow Transplantation For Leukemia Patients Conditioned With Busulfan And Cyclophosphamide : Incidence, Risk Factors and Clinical Outcome

Lee, Je-Hwan,Lee, Kyoo-Hyung,Kim, Tae-Won,Yun, Whan-Jung,Jung, Byung-Hak,Choi, Jong-Soo,Zang, Dai-Young,Kim, Sung-Bae,Kim, Sang-We,Suh, Cheolwon,Lee, Jung-Shin,Kim, Woo-Kun,Kim, Sang-Hee 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

간의 정맥폐색성질환 (veno-occlusive disease, VOD)은 황달, 통증을 동반하는 간장의 비대, 원인 미상의 체중 증가 및 복수를 동반하는 임상적 증후군으로서 골수이식술 전에 시행되는 고용량의 항암화학요법이나 방사선요법에 의하여 발생하는 질환이다. VOD의 발생 빈도는 보고자마다 차이가 있는데, 이것은 각각의 골수이식센터마다 투여하는 항암요법의 종류와 대상 환자군 및 위험 인자들이 다르기 때문이다. 저자 등은 1993년 12월 부터 1996년 10월 까지 서울중앙병원에서 busulfan 및 cyclophosphamide (BU-CY)의 투여 후 동종골수이식술을 시행 받은 24예의 백혈병 환자들을 대상으로 하여 VOD의 발생 빈도, 위험 요인 및 임상적 경과 등을 관찰하였다. 대상 환자들의 질환을 살펴보면 급성골수성백혈병 12예, 급성림프구성백혈병 5예, 만성골수성백혈병 6예 및 골수이형성증 1예 였다. 모든 환자들은 동종골수이식술 전에 busulfan 16 mg/kg 및 cyclophosphamide 120 mg/kg를 투여 받았으며, HLA가 일치하는 형제로 부터 골수를 공여받았다. 이식편대숙주질환을 예방하기 위하여 cyclosporine과 methotrexate를 투여하였고, 21예의 환자에서는 VOD 를 예방하기 위하여 heparin을 지속정주하였다. VOD는 임상적 기준으로 진단하였는데, 진단 기준은 (1) 혈중 빌리루빈 농도가 2 mg/㎗ 이상일 때, (2) 통증을 동반하는 간장의 비대가 있을 때, (3) 특별한 원인 없이 기저치의 2%가 넘는 체중의 증가 혹은 복수가 있을 때 등의 3가지 중에서 2가지 이상을 만족시키는 것으로 하였다. 12예(50%)의 환자에서 VOD가 발생하였는데 8예는 세가지 진단 기준을 모두 만족시켰으며, 4예는 2가지만 만족시키는 경우였다. VOD 발생 시기의 중앙치는 이식술 후 8일 이었으며, 중앙치 33일의 유병기간을 가졌다. 11예에서는 VOD로 부터 회복하였으며, 1예에서는 이식술 후 36일에 다기관 기능부전(multiorgan failure)으로 사망하였다. VOD의 발생에 영향을 미치는 위험인자를 분석해 보았을 때, 이식술 직전의 혈중 ALT치의 상승이 유일하게 독립적으로 영향을 미치는 인자였다. VOD 가 발생한 환자들은 다른 환자들에 비하여 혈소판 수혈 없이 2만/㎕까지 되는 기간이 유의하게 길었으며, 혈소판 수혈량이 유의하게 많았다. 이상의 결과로 BU-CY를 이용한 동종골수이식술 후 VOD의 발생율이 높음을 관찰하였으며, VOD발생의 위험인자로는 이식술 직전의 혈중 ALT치의 상승이 있었다. Veno-occlusive disease(VOD) of the liver is a clinical syndrome characterized by hyperbiliru-binemia, painful hepatomegaly and fluid retention. In bone marrow transplantation(BMT) setting, VOD is caused by dose-intensive chemotherapy and/or radiotherapy used to prepare patients for transplant. The incidence of VOD varies considerably from center to center, depending on cytoreductive therapy, the selection of the patients to be transplanted and other risk factors. The aim of this study is to determine the incidence, risk factors, and clinical outcome of hepatic VOD in leukemia patients conditioned with busulfan and cyclophosphamide (BU-CY) for allogeneic BMT. Twenty four leukemia patients underwent allogeneic BMT from December, 1993 to October, 1996 in Asan Medical Center. The underlying diagnoses were AML(12), ALL(5), CML(6) and myelodysplasia(1). The patients were prepared for BMT with busulfan (16 mg/kg) and cyclophosphamide (120 mg/kg). All patients received allogeneic marrow grafts from HLA identical siblings. Cyclosporine plus short course of methotrexate was given for GVHD prophylaxis and twenty one patients received heparin for VOD prophylaxis. VOD was clinically defined as having two of the following features: jaundice (bilirubin ≥ 2 mg/dL), tender hepatomegaly and/or unexplained weight gain( > 2% from baseline). VOD developed in twelve (50.0%) among twenty four patients. Eight patients exhibited the three standard clinical features of VOD and only two of the three features were present in fore patients. The median day of onset of VOD was day 8(range, 4 to 23) and the median duration of this complication was 33 days (range, 21 to 103). VOD has resolved in eleven of twelve patients and one patient died of multiorgan failure at day 36. When various individual characteristics such as sex, age, underlying disease, time to BMT from diagnosis, conditioning regimen, presence of hepatitis virus, the amount of pretransplant transfusion, history of liver disease, abnormal LFT at transplant, abnormal coagulation profile, and use of heparin, prophylaxis were analyzed, the only independent risk factor for the development of VOD was increased ALT level at the time of BMT. The platelet engraftment was significantly slower and platelet transfusion requirements were significantly higher in patients with VOD. In summary, our study showed high incidence of VOD in leukemia patients conditioned with BU-CY for allogeneic BMT and elevated ALT prior to BMT was an independent risk factor associated with the occurrence of VOD. In patients VOD, platelet engraftment was slower and platelet transfusion requirements were higher.

도시형자기부상열차 시스템엔지니어링 관리계획

박성환(Seong-Whan Park),김동성(Dong-Sung Kim),백수현(Su-Hyun Back),정진철(Jin-Cheol Jung),조흥제(Hung-Je Cho) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11

This study describes the SEMP(Systems Engineering Management Plan) being applied for the successful promotion of a national project of 'Urban Maglev Program'. SEMP means a kind of fundamental document for total integrated managements of the project and of the systems engineering activities. Since the tasks and responsibilities of all participants of the project, involving the tasks to collaborate between them, can be clearly defined and described in the SEMP, all participants can share and clarify their role and responsibility. The national project of 'Urban Maglev Program' are composed of three core projects; the first one for system integration, the second for the development of Maglev vehicle, and the third for a model line construction.

U-Turn 허용구간의 위치 및 적정길이 산정에 관한 연구

이정환(Lee Jung Whan),박광원(Park Kwang Won),박제진(Park Je Jin),하태준(Ha Tae Jun) 대한토목학회 2007 대한토목학회 학술대회 Vol.2007 No.10

상부 위장관을 침범한 아니사키스증 7예

김호정,이향주,신경철,김규태,이병철,조병섭,전하영,이인환,최대섭,장석일,문제웅,안재곤,권학중 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.2

The anisakiasis disease that is infected through various kinds of larvae of the anisakis family when sea fish ia eaten uncooked or half-cooked. Sinee Van Thiel, a Netherlander, found in 1960 that anisakis larvae parasitize upon the human intestinal tract of the patients who suffer from ahdomieal pain after eating herrings, there have been a number of similar reports in North America and Japan, and the clinical importance of anisakis larvae for the acute gastrontestinal infection is rising. In general, as raw sea fish is not regarded as a source of parasite infestation, the patients who had eaten it and suffered from acute abdominal pain and vomiting are considered as and to be treated of food poisoning. But it is highly possible that some of them suffer from the acute gastrointestinal symptom caused by anisakis larvae. Thereby we report on 7 examples of anisakiasis taken through endoscopic diagnosis of the patients who have the acute upper abdominal pain after eating raw sea fish.

도시형자기부상열차 SEMP 연구 (Ⅱ)

박성환(Seong-Whan Park),김동성(Dong-Sung Kim),백수현(Su-Hyun Back),정진철(Jin-Cheol Jung),조흥제(Hung-Je Cho),한석인(Suk-In Han) 한국철도학회 2010 한국철도학회 학술발표대회논문집 Vol.2010 No.7

On last year's KSR conference, the general of systems engineering technology was introduced on the focus that the SEMP(Systems Engineering Management Plan) being applied for a national project of 'Urban Maglev Program'. Since this SEMP covers very wide work scopes of 'plan, application, control of the program', 'design, analysis, test & evaluation of the system', and 'special engineering activities', it is very important that the tasks and responsibilities of all participants of the project, involving the tasks to collaborate between them, are clearly defined and described. By the point, the updating of SEMP should be done continuously. In this paper, the updating points and typically the management plan of TPM (Technical Performance Measurement), which is more concretely defined, will be explained.

도시형자기부상열차 시스템엔지니어링 기술적용

박성환(Park Seong-Whan),김동성(Kim Dong-Sung),백수현(Back Su-Hyun),정진철(Jung Jin-Cheol),조흥제(Cho Hung-Je) 한국철도학회 2009 한국철도학회 학술발표대회논문집 Vol.2009 No.11월

The general of systems engineering technology, as an effective supporting tool for the large complex system development, is introduced on the focus that the SEMP(Systems Engineering Management Plan) being applied for a national project of 'Urban Maglev Program'. SEMP means a kind of fundamental document for total integrated managements of the project and of the systems engineering activities. Since the tasks and responsibilities of all participants of the project, involving the tasks to collaborate between them, can be clearly defined and described in the SEMP, all participants can share and clarify their role and responsibility. The national project of 'Urban Maglev Program' are composed of three core projects; the first one for system integration, the second for the development of Maglev vehicle, and the third for a model line construction.

Poster Session 2 : Early nclear translocation of endonuclease G and subsequent DNA fragmentation after transient focal cerebral ischemia in mice

( Hyun Woo Kim ),( Yang Je Cho ),( Kyoung Joo Cho ),( Doo Jae Lee ),( Hyun Jung Kim ),( Byung In Lee ),( Gyung Whan Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

Poster Session 2 : Oral Presentation ; Neurobiology : Early nclear translocation of endonuclease G and subsequent DNA fragmentation after transient focal cerebral ischemia in mice

( Hyun Woo Kim ),( Yang Je Cho ),( Kyoung Joo Cho ),( Doo Jae Lee ),( Hyun Jung Kim ),( Byung In Lee ),( Gyung Whan Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2005 62회 KSBMB Annual Meeting in 2005 Vol.- No.-