경추수술의 골이식 재료로서 장골과 Biocompatible Orthopaedic Polymer(BOP)에 대한 평가

신원한,박진규,김범태,최순관,변박장 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.6

운동선수의 임장불안에 대한 연구

홍관이,이광재,김두경,부기원,노성규,한상준,박기동,오수일,문병용,이철규,박장평,엄기진,박남환 江原大學校附設 體育科學硏究所 1984 江原大學校附設體育科學硏究所論文集 Vol.- No.9

This study is designed to grasp the elements and situations of stage fright in order to propose a part of ways to cope with it. We make 511 top-class high school players, male and female, in the central districts (Seoul, Inchon, Kyungki, Chungbuk, and Kangweon Province) on object of questionnaire, and get the following results. Through players generally have all-round characters they show weakness in the superiority and emotional stability while showing strength in the sonformity. This fact comes to be related to the elements which cause stage fright. Physiological changes also have many effects on the symptom of stage fright. Especially, stage fright increases when players show sensitive reaction to the self-consciousness of final consequence which comes from the tension or uneasiness of sympathetic nerve, Thus, it turns out that players make use of reducing psychological burden by physical exercise or adjusting the level of demanded result in order to release the stage fright.

개에서 실리콘고무를 골막으로 싸서 안면골 위에 이식시 그 골막에 일어나는 조직학적 변화

박관규,강진성,함희정,한기환,최동원 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.3

Bony depression in the face may occur doe to congenital failure of development trauma or excessive surgical resection. Various materials, autogenous bone, autogenous cartilage and silicone rubber have been employed for bony contour restoration. Since autogenous bone grafts become consolidated with the underlying recipient bones, the incidence of extrusion or deviation of the graft is much fewer. But they may be easily fractured at the time of carving because of their rigidity. Autogenous cartilage grafts do not require contact with the nasal bony framework, but they tend to curl and bend. Using silicone rubber is a simple and fast procedure, but the prosthesis itself is a foreign body which causes occasional foreign body reaction or exposure of the prosthesis. Therefore, the materials, which have such nature as consolidation, complete contact and convenience without complication are considered to be ideal for augmentation of the bony depression. It may be an ideal material for prevention of the complication of the implants, if a new bone formation at the gratted periosteum wrapping the silicone rubber implants develops. To confirm the possibility, biological test with dogs was initiated. Six samples of periosteum and fascia wrapping the silicone rubber implants, respectively were collected from 9dogs at 8, 16 and 24 weeks. Grossly, difference between translucent periosteum and whitish fascia was noticeable at 8 weeks after implantation. However, it is difficult to distinguish between fascia and periosteum at 24 weeks. Histologically, fibrous capsule developed at 8 weeks and there was a marked thickening at 24 weeks on both periosteum and fascia. At one of 6 samples of 24 weeks periosteum, wrapping the silicone rubber implant, formed a new bone. In conclusion it is possible for the grafted periosteum wrapping the silicone rubber implants to make a new bone. Therefore the periosteum and fascia wrapping the silicone rubber are well taken at the recipient site. Also, they play a good role of barrier against exposure of implant. Especially the periosteum is better than the fascia, because of its bony formation and easier obtaining

요골동맥과 복재정맥을 이용한 관상동맥우회술의 조기성적

김관창,원태희,한재진,최수승,안재호 이화여자대학교 의과학연구소 2008 EMJ (Ewha medical journal) Vol.31 No.2

Background:Increasing interest and use of arterial conduits is based on the better patency of left internal thoracic artery(LITA) than that of saphenous vein(SV) graft. We compared the early result of coronary artery bypass grafting(CABG) using LITA and radial artery(RA group) with CABG using LITA and SV only(SV group). Material and Method:We compared the early operative results of 6 cases in RA group with 18 cases in SV group selected from 24 cases that had CABG between January 2006 and December 2006. We analyzed each group on the preoperative risk factors and operative results. Results:We can’t find significant differences in clinical and hemodynamic characteristics before surgery. There were no statically significant difference between two groups in operative mortality and each morbidities(postoperative intraaortic balloon pump insertion, bleeding, stroke, perioperative myocardial infarction, wound dehiscence), respectively. However, the overall incidence of conventional CABG using cardiopulmonary bypass was higher in RA group compared to SV group(p=0.016). Accordingly, RA group had longer duration of ventilation time(p=0.004) and ICU stay(p=0.003) than SV group with statically significant difference between two groups in hospital stay. The graft patency on postoperative coronary angiography or computerized tomographic angiography at 7-14 days after operation in both group patients were 100%(includeing LITA, RA and SV). Conclusion:We had early good operative results in RA group and SV group. 관상동맥우회술에서 내흉동맥의 장기개통률이 복재정맥보다 우수하다는 것이 알려짐과 더불어 두 개의 내흉동맥을 이용한 군에서의 장기 임상 성적이 하나의 내흉동맥만을 이용한 군에서의 성적보다 우월하다 것이 알려지면서 최근에는 동맥이식편의 사용이 증가하고 있다1)2). 좌내흉동맥이 동맥이식편중 가장 많이 그리고 오래 전부터 사용되어왔고 그 장기 개통률이 매우 우수한 것으로 증명되면서, 이식편으로 사용 가능한 다른 동맥이식편에 대한 사용이 늘어나고 있는 추세이다. 좌내흉동맥 이외에 사용될 수 있는 동맥이식편으로는 요골동맥, 우내흉동맥, 우위망막동맥 등이 있다. 이 중 우내흉동맥은 좌내흉동맥과 같이 사용한 경우 장기 생존율이 높다는 보고도 있지만 가용길이가 짧아 이용에 한계가 있고 수술시간이 길어지며 상처 합병증과 기술적인 문제 등이 있다2). 요골동맥은 Carpentier 등3)에 의해 1970년대 초 처음 이식편으로 소개되었으나 추적 관상동맥조영술상 높은 이식편 부전으로 인해 사용이 중단되었다가 1989년 다시 소개되어 주목을 받게 되었고4), 요골동맥박리술의 기술적인 변형과 항연축제의 사용으로 요골동맥의 연축을 최소화하여 사용하면서 많은 저자들에 의해 좋은 임상 성적과 높은 개통률을 보이고 있다5-7). 본 이화여자대학교 목동병원 흉부외과에서도 요골동맥을 좌내흉동맥에 이은 두 번째 동맥이식편으로 사용하고 있는데 본 연구에서는 본원에서 경험한 요골동맥을 사용하여 시행한 관상동맥우회술의 임상 성적과 수술 후 관상동맥조영술 혹은 컴퓨터 단층 혈관조영술 결과를 분석해 보고 아울러 이를 요골동맥을 사용하지 않고 내흉동맥과 복재정맥만을 이용한 군의 성적과 비교 분석해 보았다.

골다공증 환자 혈청이 정상인 조골세포의 성장과 분화에 미치는 영향

김진,이재훈,김경욱,이광원,한상배,김환묵 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.4

Osteoporosis is a condition in which an imbalance appears between bone resorption and formation, with bone resorption exceeding formation. Recent studies have shown that bone formation abnormalities in various forms of osteopenia result mainly from defective recruitment of osteoblastic cells. These abnormalities in osteoblast function and bone formation are associated with alterations in the expression or production of several growth factors, such as TGF-β which modulate the proliferation and activity of bone-forming cells. Bone transplantation is an absoulte requirement in several patholigical conditions. The growth factors, such as TGF-β, PDGF, were the effective in promoting growth of the bone in vitro and in animal models. We have investigated the effects of PDGF and TGF-β on the proliferation and differentiation of the normal human osteoblast in vitro culture. The normal human osteoblast from iliac bone were primarily cultured. The serums obtained from the osteoporotic patients and the normal was used to quantify PDGF and TGF-β from the osteoporotic patients serum and the normal serum. To clarify the effects of the various different the culture conditions such as 1×10 exp(4) cells/㎖, 2.5×10 exp(4) cells/㎖, 5×10 exp(4) cells/㎖, 10×10 exp(4) cells/㎖ (0.2∼2×10 exp(4) cells/well) of the osteoblast at 24 hours, 48 hours, 72 hours under 10% FBS, 10% normal human serum, 10% osteoporotic human serum, 3% normal human PRP, 3% osteoporotic human PRP. The cell proliferation and differentiation was determined by [^3H]-thymidine and SRB assay, and the magnitude of differentiation to osteoblast was confirmed by von Kossa staining and alkaline phosphatase stain and measuring the alkaline phosphatase activity during 48 hours and 72 hours. Statistical differences were evaluated using the scheffe's test. The ANOVA procedure of the SAS system. The obtained results were as follows ; 1. The age distribution of normal human was 36.9±4.4 old, osteoporotic human was 72.5±0.2 old with statistical significant difference(p<0.05). 2. The quantification of TGF-β of the normal human serum was 39658.38±11630.43 pg/㎖, the osteoporotic human serum was 30459.40±1704.92 pg/㎖ with statistical significant difference. The quantification of PDGF of the normal human serum was 3064.13±709.51 pg/㎖, the osteoporotic human serum was 2514.13±140.21 pg/㎖ with no statistical significant difference(p<0.05). 3. The DNA synthesis and protein assay of human osteoblast at 24 hours, 48 hours, 72 hours was similar increased to 10% normal human seurm, 10% osteoporotic human serum. There was no statistical significant difference between the normal human and the osteoporotic patients in 3% normal human PRP and 3% osteoporotic human PRP. 4. The optimal cell concentration was 5×10 exp(4) cells/㎖ among 1×10 exp(4) cells/㎖, 2.5×10 exp(4) cells/㎖, 5×10 exp(4) cells/㎖, and 10×10 exp(4) cells/㎖. The DNA synthesis was decreased after 72 hours in the normal human serum and PRP, the osteoportic serum and PRP. 5. The alkaline phosphatase activity was as the same result 10% FBS, 10% osteoportic serum and 10% normal human serum at 48 hours with no statistical significant, but the alkaline phosphatase activity was increased in 10% osteoportic human serum and 10% normal human serum except 10% FBS at 72 hours. From above result, the amount of TGF-β of the normal human growth factor was higher than the osteoporotic patients, but the growth factors of the osteoportic patients were enough the proliferation and differentiation of normal human osteoblasts such like the same effects of normal human growth factors.

혈행차단에 따른 두개골외판 이식의 생존 차이

박관규,박성근,한기환,강진성 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.1

Difficulties arise in prediction of maintenance of the graft volume and viability of graft over time when a bone graft used for facial reconstruction. A bone-to-bone contact between the graft and the recipient bone is imporant for creeping substitution and survial of the recipient bone is important for creeping substitution and of the grafted bone. An adequate blood supply is also essential to ensure the survival of any live cells of the surface of the graft. Our study was designed to determine which one is an important factor viability of the grafted bone in Korean adult dogs : a bone-to-bone contact or overlying soft tissue. Blocks of outer table of the parietal bone were placed at the maxillae subperiostially in 4 different ways : bone-to-bone contact groups(groups Ⅰand Ⅱ)with placing a silicone membrane over the grafted bone and soft tissue contact groups(groups Ⅲand Ⅳ)with placing a silicone sheet between the grafted bone and the recipient. In groups Ⅰand Ⅲ, the cancellous surfaces of the parietal bone was placed on the recipient and the cortical surfaces were placed on the recipient in groups Ⅱ and Ⅳ. Caliper techniques were used to study the rates of volume maintenance of the grafts at 6, 12, and 20 weeks after bone grafting. The volumes of the living bone were quantified microscopically using a modified point-countiong technique. The volume is reduced in a similar rate with time in all groups. At 6 week, living bone cells increased in soft tissue contact groups Ⅲ and Ⅳ however, and increased in bone to bone contact groups Ⅰand Ⅱ at 12 and 20 weeks. there were osteoblastic proliferation and laminated mature bones in group Ⅰand Ⅱ. But osteoclasts and their associated osteolytic changes were still seen near the silicone membrane in group Ⅲ and Ⅳ, which may imply a continuing resorptive process with time. In summary, revascularization from the overlying soft tissue is important for the graft survival in early stage of the bone grafting while bone-to-bone contact may be essential in a later stage.

금속사출성형에 의한 중합금 소결체 부품 제조에 관한 연구

여환균,김병진,김길수,김성훈,송준호,한관희 嶺南大學校 工業技術硏究所 2003 工業技術硏究所論文集 Vol.31 No.2

In this study we demonstrate that a small complex part of a tungsten-based heavy alloy (W-Ni-Cu) can be readily manufactured by utilizing the metal injection molding (MIM) technology. We use a simple thermoplastic binder system, containing low density polyethylene, paraffin wax, and stearic acid. To remove the organic binder from the molded part, we employ the solvent extraction and thermal debinding processes. Densification of debound parts is achieved by sintering in hydrogen atmosphere. The experimental results of the optimization of process variables to manufacture the heavy alloy part are described, with a brief discussion.

공원묘지 유실에 의한 대량재해의 개인식별

강신몽,이한영,윤중진,이원태,김종열,이영석,서재관,최득린,서지석,이혜경 大韓法醫學會 1992 대한법의학회지 Vol.16 No.1

Individual identification of severely decomposed or skeletonized bodies is an important part in medicolegal field. 142 bodies were torn up and carried away by flood in a park cemetry, Kwang-Ju, Kyunggi province, Korea at Sept., 10, 1990. The authors examed the 56 bodies, which were gained, through medical, anthropological, odontological, radiological methods, and superimposition for individual identification. Among them, 41 cases were successfully identified, and remaining 15 cases were not identified because of sever destruction and no adequate information.

접합면의 차이에 따른 두개골 외판의 생존

박성근,송중원,한기환,강진성,박관규 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.3

Onlay graft of the calvarial bone has been popularized in craniofacial surgery because absorption of the calvarial bone is less than that of the endochondral bone. But the problems with using the calvarial bone are its rigidity and difficulty in setting a precise apposition. When the outer tables of the calvarial bone are used for augmentation and reconstruction of the convex zygoma, forehead, or chin, it is better to place the cancellous surface over the convex recipient bone to get a precise apposition. Whereas, it is better to place the cortical surface over the concave nose or orbital cavity to achieve a good apposition. Therfore, our study was designed to determine the differences of bone absorption and regeneration between cancellous and cortial bone contact to facial bone, and between preserved periosteum and detached periosteum in autograft of calvarial bone in dog models. Outer tables of the calvarial bone were placed in subperiosteal pockets of the upper and lower maxilla in four different ways : Group I ; The cancellous surface was placed in contact with the bare recipient bone, and the cortical surface attached with periosteum was accordingly contacted with the elevated periosteum of the recipient bone. Group Ⅱ ; The corical surface attached with periosteum was placed in contact with the bare recipient bone and the cancellous surface was contacted with the elevated periosteum of the recipient bone, Group Ⅲ ; The arrangenent was similar to Group Ⅰ except that the periosteum of the graft was deprived. Group Ⅳ ; The arrangement was similar to Group Ⅱ except that the periosteum of the graft was deprived. Volume measurements using a caliper technique and histological study were made 20 weeks postoperatively. The volume of maintenance is as follows ; Group Ⅰ, 84.2% ; Group Ⅱ, 77.6% ; Group Ⅲ, 77.0%, and Group Ⅳ, 69.5%. The histolgical contribution of living bone was assessed by a modified point counting technique : Group Ⅰ, 86.6%, Group Ⅱ, 83.8% ; Group Ⅲ, 79.6% and Group Ⅳ, 77.6%. Greater volume maintenance and histological contribution of living bone were found when cancelllous surface rather that the cortical were placed in contact with the recipient bone and the grafts from their periosteum were preserved. We concluded that in order to expect better survival of a grafted bone, the cancellous surface of the graft should contact with the recipient bone and that the periosteum of the graft should be preserved.

개선된 사람 혈장중 세파클러 농도 정량법을 이용한 세파클러 캡슐의 생체이용률 측정

김태완,송옥경,한선영,Cao, Qing-Ri,박미진,강성화,신관석,Cui, Jing-Hao,이범진 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

After establishing improved HPLC analytical method ofcefaclor in human plasma samples, a bioavailability study of cefaclor capsules was conducted according to the guidelines of Korea Food and Drug Administration (KFDA). The standard calibration curve using an HPLC with UV detector was constructed in a range of 0.0324--16 μg/m1. The 6% perchloric acid instead of 6% trichloroacetic acid was used to precipitate plasma protein. The HPLC chromatograms were precisely and accurately resolved when spiked with human plasma spiked with cefaclor and cephalexin (internal standard). Twenty healthy male Korean volunteers received two commercial cefaclor capsules, Neocef ' capsule (Jinyang Pharm. Co., Ltd) or Ceclor" capsule {Lilly Korea. Co., Ltd.) at the 250 mg cefaclor in a 2 x 2 crossover study. There was a one-week washout period between the doses. Plasma concentrations of cefaclor were monitored for 8 hours after oral drug administration. AUC, the area under the plasma concentration-time curve from time zero to 8 hr (13 points), was calculated by the linear trapezoidal rule method. C,,,a" (maximum plasma drug concentration) and Tmax (time to reach Cma,) were compiled from the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed AUC, and Cmax. No significant sequence effect was found for all of the bioavailability parameters indicating that the cross-over design was properly performed. The 90% confidence intervals of the AUC, ratio and the Cmax ratio for Neocer/Ceclor" were 0.9049 S S < 1.0304 and 0.9776 5 S 1.226, respectively. These values were within the acceptable bioequivalence intervals of 0.80-1.25. Thus, our study demonstrated the bioequivalence of Neocef"/Ceclor" with respect to the extent of absorption.