한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

진달래 꽃에 의한 Grayanotoxin 중독 3 례

김아진,김준식,신동운,백광제,한승백,이용주 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Traditionally, the Rhododendron species has been used in gastrointestinal disorder or hypertension. Grayanotoxin exists in honey, flowers, pollen, and the nectar of the Rhododenron species. We experienced 3 cases of Grayanotoxin intoxication. The symptoms of intoxication were nausea, vomiting, hypotension, bradycardia, diplopia, dizziness, and chest discomfort. Generally, the treatment for Grayanotoxin intoxication is fluid resuscitation and injection of atropine sulfate. The patients who were intoxicated with Grayanotoxin were discharged without complication after supportive care.

피로와 의료이용, 업무상 사고 및 질병결근

장세진,고상백,강동묵,김성아,정진주,이철갑,강명근,현숙정,조정진,차봉석,박종구 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study examined the relationship of fatigue to medical utilization, occupational accident and sickness absence. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002~2004). A total of 30,146 employees categorized within the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were recruited. A structured-questionnaire was used to assess the participants' sociodemo-graphics, job-related factors, health-related behaviors, personality trait, self=perceived fatigue (MFS) and the frequency of medical utilization, occupational accident and sickness absence. Data on medical utilization and occupational accident were confirmed from company health records. Chi-square test and logistic regression analysis were used to elucidate the relationship of self-perceived fatigue to medical utilization, occupational accident and sickness absence. Results: In logistic regression analyses, self-perceived fatigue was associated with medical utilization (outpatient), occupational accident and sickness absence although the strength of the associations was higher in the blue collar workers except for medical utilization. For medical utilization, the white collar worker's adjusted odds ratios of outpatient utilization for workers scoring in the third quartile and the highest quartile, compared to workers scoring in the lowest quartile were 1.50 (95% CI: 1.20-1.87) and 2.16 (95% CI: 1.69-2.75), respectively. The blue collar workers' adjusted odds ratios of outpatient utilization for workers scoring in the second (OR: 1.23, 95% CI: 1.06-1.43), third (OR: 1.42, 95% CI: 1.22-1.66) and highest quartile (OR:1.83, 95% CI: 1.55-2.17), respectively, compared to workers scoring in the lowest quartile were statistically significant. However, no associations were found between fatigue and inpatient utilization for either the white or blue collar workers. The blue collar workers' adjusted odds ratios of being injured for workers scoring in the second, third and highest quartile were 1.41 (95% CI: 1.00-2.00), 1.57 (95% CI: 1.10-2.22) and 2.41 (95% CI: 1.69-3.44), respectively, compared to those scoring in the lowest quartile, but, those of the white collar workers were not significant. There was more likely to an increased risk of sickness absence in workers scoring in the second, third and highest quartile compared to those scoring in the lowest quartile in both white and blue collar workers, but the magnitudes of risk were higher in the blue collar workers than the white collars workers. Conclusion: This result suggests that fatigue is a determinant predictor of medical utilization, occupational accident and sickness absence, and that the pattern of risks for the three outcomes differs according to the occupational type. Some limitations of this study and considerations for future study were also discussed.

A comparative analysis of rumen pH, milk production characteristics, and blood metabolites of Holstein cattle fed different forage levels for the establishment of objective indicators of the animal welfare certification standard

Baek Dong Jin,권현철,Mun Ah Lyum,Lim Joo Ri,Park Sung Won,Han Jin Soo 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.1

Objective: This study was conducted to obtain an objective index that can be quantified and used for establishing an animal welfare certification standard in Korea. For this purpose rumen pH, ruminating time, milk yield, milk quality, and blood components of cows reared in farms feeding high forage level (90%) and farms feeding low forage level (40%) were compared. Methods: Data on rumen pH, rumination time, milk yield, milk fat ratio, milk protein ratio, and blood metabolism were collected from 12 heads from a welfare farm (forage rate 88.5%) and 13 heads from a conventional farm (forage rate 34.5%) for three days in October 2019. Results: The rumination time was longer in cattle on the welfare farm than on the conventional farm (p<0.01), but ruminal pH fluctuation was greater in the cattle on conventional farm than the welfare farm (p<0.01). Conventional farms with a high ratio of concentrated feed were higher in average daily milk yield than welfare farms, but milk fat and milk production efficiency (milk fat and milk protein corrected milk/total digestible nutrients) was higher in cattle on welfare farms. Blood test results showed a normal range for both farm types, but concentrations of total cholesterol and non-esterified fatty acid were significantly higher in cows from conventional farms with a high milk yield (p<0.01). Conclusion: The results of this study confirmed that cows on the animal welfare farm with a high percentage of grass feed had higher milk production efficiency with healthier rumen pH and blood metabolism parameters compared to those on the conventional farm. Objective: This study was conducted to obtain an objective index that can be quantified and used for establishing an animal welfare certification standard in Korea. For this purpose rumen pH, ruminating time, milk yield, milk quality, and blood components of cows reared in farms feeding high forage level (90%) and farms feeding low forage level (40%) were compared.Methods: Data on rumen pH, rumination time, milk yield, milk fat ratio, milk protein ratio, and blood metabolism were collected from 12 heads from a welfare farm (forage rate 88.5%) and 13 heads from a conventional farm (forage rate 34.5%) for three days in October 2019.Results: The rumination time was longer in cattle on the welfare farm than on the conventional farm (p<0.01), but ruminal pH fluctuation was greater in the cattle on conventional farm than the welfare farm (p<0.01). Conventional farms with a high ratio of concentrated feed were higher in average daily milk yield than welfare farms, but milk fat and milk production efficiency (milk fat and milk protein corrected milk/total digestible nutrients) was higher in cattle on welfare farms. Blood test results showed a normal range for both farm types, but concentrations of total cholesterol and non-esterified fatty acid were significantly higher in cows from conventional farms with a high milk yield (p<0.01).Conclusion: The results of this study confirmed that cows on the animal welfare farm with a high percentage of grass feed had higher milk production efficiency with healthier rumen pH and blood metabolism parameters compared to those on the conventional farm.

국내,외 줄기세포은행 현황

백진아 ( Jin Ah Baek ),최영민 ( Young Min Choi ),문신용 ( Shin Yang Moon ) 서울대학교 인구의학연구소 2012 人口醫學硏究論集 Vol.25 No.-

Human embryonic stem cells (hESCs) have great potentials as a source of cells for cell-based therapies and can be used to develop a system such as toxicology and drug discovery. Human ES research is being conducted with a variety of cell lines and culture conditions, however, the consequence of using such conditions would be confuse in scientific improvement. For this reason, the effort for cell stability and safety used in research work has been recognised and developed by international groups worked for stem cell banking. High-quality stem cell lines confirmed by quality assurance/control and ethically sourced are needed for invaluable stem cell researches and therapies.

인간 전분화능 줄기세포 Non-coding RNAs의 기능

백진아 ( Jin Ah Baek ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2015 人口醫學硏究論集 Vol.28 No.-

Human pluripotent stem cells (hPSCs) with the capacity of self-renewal and multilineage differentiation are promising sources for replacement therapies and regeneration medicine. Many researches have been spent to undersrand rhe molecular mechanisms underlying hPSCs mainrenance and pluriporency, and ir is clear rhar borh rranscriprional and epigeneric levels of regularion have crucial roles. Recent research indicares rhar regularorynon-coding RNAs (ncRNAs) are key regulators of hPSCs epigeneric nerwork. For rhis reason, we summarize rhe findings that focus on regulatory ncRNAs of human embryonic stem cells (hESCs) and human induced pluripotent srem cells (hiPSCs).

인간 배아줄기세포와 인간 역분화줄기세포의 유전체 수준 비교

백진아 ( Jin Ah Baek ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2013 人口醫學硏究論集 Vol.26 No.-

Human pluripotent stem cells (hPSCs), which include human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), can self-renew while maintaining the pluripotency. They have been a prime candidate cell source for the development of cell replacement therapies and regenerative medicine. hESCs and hiPSCs are similar in morphology, cell-surface marker and teratoma formation capacity. However, questions remain on whether the epigenetic reprogramming is complete. For this reason, in this study, we compared the transcriptom and epigenetic properties between hESCs and hiPSCs.

Xeno-free 환경에서 인간 배아줄기세포 확립에 관한 연구 동향

백진아 ( Jin Ah Baek ),최영민 ( Young Min Choi ),문신용 ( Shin Yong Moon ) 서울大學校 醫學硏究院 人口醫學硏究所 2009 人口醫學硏究論集 Vol.22 No.-

인간 전분화능줄기세포의 대량 배양을 위한 마이크로캐리어(microcarrier) 배양 시스템

배진아 ( Jin Ah Baek ),최영민 ( Young Min Choi ) 서울대학교 인구의학연구소 2014 人口醫學硏究論集 Vol.27 No.-

Human pluripotent stem cells (hPSCs) have been a prime candidare cell source for the development of cell replacement therapies and regenerative medicine because they can self-renew and maintain the pluripotency. The use of hPSCs for clinical applications requires a scalable, continuous propagation and controlled process for clinically relevant quantities. However, the conventional culture techniques of hPSCs are two-dimensional(2D) systems, which are associared with low production yields. In order to overcome these issues, several groups have developed to culture hPSCs on microcrriers and they demonstrated that is more robust and feasible for scale-up than traditional 2D cultures. The cultivation of hPSCs on microcarrier culture system is relatively novel in the field of stem cells, so more researches are needed in several critical issus: coatings, medium development and improved bioprocess parametecrs for opcimal hPSCs systems.

새로운 인간 배아줄기세포의 미분화 마커에 대한 고찰

백진아 ( Jin Ah Baek ),최영민 ( Young Min Choi ),문신용 ( Shin Yong Moon ) 서울대학교 인구의학연구소 2011 人口醫學硏究論集 Vol.24 No.-

The pluripotency and self-renewal of human embryonic stem cells (hESCs) provide appropriate systems for the development of cell replacement therapies. Recent studies have converged on the finding transcription factors, such as Oct4, Sox2, and Nanog, which are served as master regulators in the maintenance of undifferentiated hESC or in early stages of differentiated hESCs. However, downstream targets and signals of these transcription factors for hESC maintenance and regulation are not well characterized. In this study, we summarize the current novel pluripotency markers of hESCs, L1TD1, Banf1, E1B-AP5, POLR3G and L1CAM, that were published in 2011. The identification of novel hESC markers is essential for understanding hESC pluripotency and the mechanisms involved in hESC differentiation and self-renewal.