Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

항암화학요법을 받는 암 환자에 대한 Nucare^R의 영양지지효과

조덕연,김현수,곽상혁,강정현,김철희,배광봉,김종숙,박상준,윤환중,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Purpose : The purpose of this study was to evaluate the effect of nutritional support with enteral liquid supplement in cancer patients receving chemotherapy for possible benefit in nutritional, immunologic and golbal function of patients. Patients and Methods : From October 1995 to February 1997, 30 advanced cancer patients receving chemotherapy were divided two roups. The Nycare group, in addition to normal diet, Nucare^R enteral supplement was given for 1week right after chemotherapy for the duration of 2 chemotherapy cycles. Control group received only normal diet without parenteral fluid supplement for 2 chemotherapy cycles. Results : Median ages were 53 end 56 years for Nucare group and Contrl group respectively. Performance scores was less than 2 by ECOG scale. All patients were stage Ⅲ or Ⅳ. The physical parameters, such as weight, arm muscle circumference(AMC) and triceps skin fold(TSF) were decreased in both groups after 2 cycles of anticancer therapy. but it was less severe in Nucare group(p<0.05). Serum transferrin was maintained in mild deficit state in Nucare group, whereas it aggravated form mild to moderate deficit in Control group(p<0.05). Serum albumin level increased in Nucare group without statistical significance. but it decreased from normal to mild deficit in Control group. Serum total protein did not change significantly in Nucare group. but in Control group, serum total protein was decreased from 7.24±0.9 to 6.52±0.5(P<0.05). Total lymphocyte count did not change significantly in both groups. Conclusion : This study shows that the nutritional support with Nucare^R was effective in the prevention of nutritional deficit status in patients receving a nticancer chemotherapy.

의미 분석 단계에서의 정보 흐름 제어

조현욱,김명수,정은환,유재휘,윤병주 명지대학교 공학기술연구소 1986 공학기술연구소 논문집 Vol.1 No.-

One of protection methods for computer internal data is to pursue data flow in program and to control its flow. In this thesis, we propose a mechanism which guarantees secure information flow in a program. By investigating security classes of all the variables in a statement and judging necessary condition for secure information flow, proposed model descriminates the program whether it is secure or not. Out model can be implemnted at language translation time, particularly semantic analysis phase, by pursuing security class transfer of each statement.

소형어선의 자동항행시스템을 위한 전자자기컴퍼스의 실용화 연구

조현정 도립 강원전문대학 2000 道立 江原專門大學 論文集 Vol.3 No.-

In order to study basic information on the developed of an electromagnetic compass for autopilot system of small type fishing boat, experiments were carried out the varies of the compass error on ship's head up bearing with magnetic compass and electromagnetic compass on berthing at the pier. The varies of compass error on ship's head up bearing of the wooden fishing boat, turned on attracting fish lamps of power consumption 85㎾, steering compass got westerly compass error with 7°, electromagnetic compass got westerly compass error between 13° and 16°. The F.R.P. fishing boat, turned on attracting fish lamps of power consumption 130㎾, electromagnetic compass got easterly compass error between 19° and 23°. The steel fishing boat, turned on ship's navigation equipments of power consumption 225㎾, steering compass got westerly compass error with 13°, electromagnetic compass got westerly compass error with 68° on the upper deck, westerly compass error with 16° on the forecastle deck, westerly compass error with 16° on the forecastle deck, westerly compass error with 32° on the wheel house, westerly compass error with 20° on the compass deck.

청소년의 구매 행동과 관련 변인에 관한 연구 : 강원 지역 중학생을 중심으로 Focused on the Middle School Students in Kangwon Province

조현주,최동숙 한국 가정과 교육 학회 2002 한국가정과교육학회지 Vol.14 No.1

The purpose of this study is to find out the level of purchasing behavior and related variables of Korean adolescents in Kangwon province, and to suggest a direction of consumer education for rational purchase and of Home Economics Education, through noticing the influence of socio-demographic variables and socialization-related variables. To find out the results of this study, a survey was made using questionnaires with middle school students who live in Kang-won province, and 472 questionnaires were used as the analysis data, The methods of statistical analysis used for this study were Grobach'α. Frequency Distribution. Percentile, mean. Standard Deviation, ANOVA, t-test. Duncan's Multiple Range Test. Multiple Regression Analysis, and also SPSS PC+ was used. The summaries of the results of this study were as follows. First, the level of the adolescents' purchasing behavior was the average 3.34 which is higher than the centered number 3. and the level of pre-purchasing behavior(3.36) was higher than that of the post-purchasing behavior(3.29). Second, the adolescents' purchasing behavior showed the significant difference according to the mothers' educational level and family income. Third, among the socialization-related variables, the group which had much communications with their parents and friends about purchase, the group which got much consumer education in school and their home, the group which can see much advertisements about purchase showed high level of purchasing behavior. Fourth, in the regression analysis, the consumer education in school, the communication with parents or friends were significant variables to the level of purchasing behavior. Looking into the subcategory, the communication with parents, the amount of pocket money, the father's occupation were significant variables to the level of pre-purchasing behavior. And the consumer education in school, the communication with parents or friends, the father's occupation were significant variables to the level of post-purchasing behavior.

스트룹 테스트와 단순반응 테스트의 훈련 향상도에 관한 연구

조현우,박영민,박사엘,공추,왕초전,장신,김재화 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study which of the Stroop test training and simple response training can reduce the Stroop effect in the information processing process when continuous training is performed. 4 male students from S University(Asan-si) were divided into Stroop test training group(2) and simple reaction group(2). The test was conducted 10 times a day, and average was obtained and analyzed. The results encountered through this study are as follows. 1 The simple response training group improved more slowly than the Stroop test training group. 2. In the Stroop test training group, the rate of increase increased significantly compared to the simple response training group, but it did not increase once the record reached a certain point. 3. Simple response training at increasing values ​​was also trained on the Stroop effect. 4. In the average of phage values, both groups look similar.

마이크로 밀링시스템에서 레버구조의 피에조구동기 설계

고태조,정병묵,양현모,박종권 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

High precision machining technology has become one of the important parts in the development of a precision machine. Such a machine requires high speed on a large workspace as well as high precision positioning. For machining systems having a long stroke with ultra precision, a micro stage system with lever mechanism is proposed in this paper. The proposed micro stages are activated by the piezo-actuators with high precision, however, they have severe disadvantage for the travel range that the stroke is limited to a few microns. Because it is not easy to increase the stroke of piezo-actuator, it is very difficult to make an X-Y stage with only piezo-actuators. Therefore, in order to increase it, this paper proposes a lever mechanism for the piezo-actuator stage. In the experiments, we can confirm that the stroke is increased more than three times and it is possible to implement the micro X-Y stage using piezo-actuators.

성당 미사공간의 실내음향특성에 관한 실험적 연구

박현구,조경숙,김선우 대한건축학회 2003 대한건축학회논문집 Vol.19 No.2

This study has performed to analyse the measurement the main hall of Catholic churches and to find out general room acoustical characteristics. Furthermore, extracted data could be used in subjective evaluation for optimum values of each physical parameter. For the reason, we surveyed whole Catholic churches located in Gwangju metropolitan city and finally chose 11 churches to measure. Room volume of buildings chosen is ranged widely from 1,782 to 9,195 cubic meter so that measured values can be distributed including various characteristics of acoustical parameters. As a result, the differences of values using PA system as sound source were larger than those using omnidirectional speaker in the center part of alter and values of D50 and C80 in the balcony floor were especially appeared to have different values comparing with those of main floor due to diversified layout of speakers. The range of RT and EDT was from 1 to 3 sec. approximately depending on the room volume and sound absorbing materials installed on floor, wall and ceiling and it was revealed that RT at 250㎐ of center frequency range was shortest and RT at 1㎑ was largest as most of the materials applied to finishing were panel absorbers and reflectors such as wood panel, concrete and brick.

전문가 제어기를 이용한 단결정(Al₂O₃) 성장 제어

조현섭,민병조 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

Robust control far DC motor is needed according to the highest precision of industrial automation.It is a quite quality concerning to control temperature of single crystalline growth as it does when you get most of heat treating products.It is also important factor to control temperature when you make the A1203(single crystalline) used to artificial jewels, glass of watches, heat resistant transparent glasses.Thus, it is a major interest to get the proper temperature in accordance with the time process while you are making mixture of oxygen and hydrogen to have the right temperature.In this paper, we will study of electrical valve positioning system for the gas mixture to improve the quality of products.

Sequence sampling에 의한 단결정(Al_2O_3) 성장 제어

조현섭,송용화,민병조 호서대학교 반도체제조장비국산화연구센터 2000 학술대회 자료집 Vol.2000 No.1

It is a quite quality concerning to control temperature of single crystalline it does when you get most of heat treating products. It is also important control temperature when you make the Al203(single crystalline) used to jewels, glass of watches, heat resistant transparent glasses. Thus, it is a ma, to get the proper temperature in accordance with the time process while making mixture of oxygen and hydrogen to have the right temperature. In we will study of electrical valve positioning system for the gas mixture to im quality of products.