피임제(Norinyl)가 흰쥐 난소의 Alkaline Phosphatase 활성에 미치는 영향

신경숙,이군자,정호삼,이규식 대한해부학회 1985 Anatomy & Cell Biology Vol.18 No.1

In mammals, female sexual hormones secrete at different rate during the sexual cycle so that the follicular cells and the granulosa cells undergo structural and functional changes. Therefore the authors undertook to pursue the changes of the graafian follicle during the estrous cycle and the effect of the oral-contraceptive. Female Sprague-Dawley rats weighing 150g were used as a experimental animal. Vaginal smears were obtained daily and only animals that showed consecutive four-day estrous cycle were used. The animals of the oral-contraceptive administered groups were administered norinyl, dissolved in distilled-water. The ovary was fixed with 10% neutral formalin and sliced at 14 ㎛ thickness in a frozen cryostat. The activity of alkaline phosphatase was observed by the Gomori's method for histochemical study. The other ovary was homogenized and the homogenate was assayed by the Lowry's method for biochemical study. The results were as follows. 1) In the follicle alkaline phosphatase activity was positive in the theca layer while the granulosa layer was negative. Strong activity were shown at proestrous and metestrous stage during the estrous cycle. And alkaline phosphatase activity of the ovary did not change markedly during the estrous cycle but the activity of estrous stage were slightly higher than other stages. 2) In the norinyl-administered group, the alkaline phosphatase activity of the ovary did not change during the estrous cycle. And the activity of the administered groups was lower than the control group.

급성 복증 소견을 보인 신증후출혈열 1예

신호식,정연순,임학 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Hemorrhagic fever with renal syndrome (HFRS) frequently cause abdominal pain with tenderness; therefore this is occasionally misdiagnosed as surgical abdomen. A 20-year-old male presented with abdominal pain for 2 days. He also had suffered from fever since 7 days ago. Abdominal computed tomography showed ascites along both paracolic gutters, mesentery and paravesical spaces. Hantaan virus infection was demonstrated by serology. After conservative treatment, the patient with HFRS spontaneously improved. We report a case of HFRS presented acute focal abdominal pain of acute abdomen.

혈청 Cystatin C와 크레아티닌에 근거한 사구체 여과율의 비교

신호식,정연순,임학 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background : In recent years, cystatin C (CysC) was proposed as a new marker for the glomerular filtration rate (GFR) due to a constant serum level. The present study analyzes whether creatinine based GFR prediction equations might be replaced by simple prediction equations based on serum concentrations of CysC in healthy young Korean men. Methods : We studied 145 apparently healthy young Korean men aged 19-29 years who visited The Armed Forces Yangju Hospital (Gyeonggi Province, Repulic of Korea) for health check from November 2008 to January 2009. Creatinine-based GFR was estimated by the formula of modified MDRD, Crockcroft Gault (CG). CysC-based GFR was estimated by the formula of Larsson, Hoek, Filler, Grubb, Le Bricon, Orebro-cyst (DAKO), Orebro-cyst (Gentian). Linear regression analysis was performed to evaluated correlations between GFR estimating formulae based upon serum CysC and serum creatinine. Results : Among CysC based GFR, Hoek's GFR is most similar with CG GFR and abbreviated MDRD GFR. Significant correlations were noted between CysC based GFR and creatinine based GFR. Conclusion : Creatinine based GFR prediction equations for healthy young Korean men might be replaced by simple prediction equations based on serum concentrations of CysC (especially Hoek' equation). 배경 : 혈청 cystatin C는 외부 요인에 영향을 받지 않고 일정한 값을 유지하므로 사구체 여과율에 대한 새로운 표지자로 제시되고 있다. 본 연구는 젊은 성인 남성에서 cystatin C에 근거한 사구체 여과율이 혈청 크레아티닌에 의한 사구체 여과율을 대체할 수 있는지 조사하였다. 방법 : 2008년11월부터 2009년1월까지 병원을 방문한 평균 연령 21세의 젊은 성인남성 145명을 대상으로 하였다. Cystatin C에 근거한 사구체 여과율은 Larsson, Hoek, Filler, Grubb, Le Bricon, Orebro-cyst (DAKO), Orebro-cyst (Gentian) 공식을 이용하였고 혈청 크레아티닌에 근거한 사구체 여과율은 변형된 MDRD, Crockcroft Gault 공식을 이용하여 산출하였다. Cystatin C에 근거한 사구체 여과율과 혈청 크레아티닌에 근거한 사구체 여과율은 선형 회귀분석을 통해서 연관 관계를 확인하였다 결과 : Cystatin C에 근거한 사구체 여과율 산출 공식중에서 Hoek 공식에 의한 사구체 여과율이 혈청 크레아티닌에 근거한 사구체 여과율과 의미있는 상관관계를 보였다. 결론 : 젊은 성인 남성에서 Cystatin C에 근거한 사구체 여과율은 혈청 크레아티닌에 의한 사구체 여과율을 대체할 수 있을 것으로 생각된다.

박막 크로멜-알루멜 열전퇴형 교류-직류 변환기

정인식,김호운,김진섭,이정희,이종현,신장규,박세일,권성원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

A planar chromel-alumel multijunction ac-dc converter was fabricated on a LPCVD Si_(3)N_(4)/SiO_(2)/Si_(3)N_(4)-diaphragm, prepared by silicon bulk micromachining, which thermally isolated a bifilar evanohm-heater and the hot junctions of a chromel-alumel thermopile from the silicon substrate. The voltage responsivity, the ac-dc transfer error, and the fluctuation of the output thermoelectric voltage of the converter were investigated. The respective voltage responsivities in air and in a vacuum of the converter were about 3.16 mV/mW and 9.41 mV/mW. The ac-dc voltage and the current transfer errors in air were about ±1.5 ppm and ±0.7 ppm in a frequency range from 10 Hz to 10 kHz, respectively. The fluctuation of the output thermoelectric voltage from 5 seconds to 120 seconds after preheating in air for 5 seconds was about 0.06 % for a heater input of dc 1 V.

Mitomycin이 Mouse 간장의 인산염분해 효소에 미치는 영향

정호삼,신규석,이군자,이규식 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1

Mitomycin (MC), a wide spectrum antibiotic and anticancer drug, is isolated from streptomyces caespitosus. MC decomposes DNA in cancer cell by forming a complex with DNA and inhibits division of cancer cells by interfering with the biosynthesis of DNA. In the normal cell, MC cross linkes with DNA and inhibits synthesis of RNA and protein. Therefore MC causes many complication in the MC treated patient. The authers have investigated the effect of MC on the mouse liver histochemically observing the change in the activities of alkaline phosphatase and ATPaes. Male mouse, DDY strain, weighing about 20 grams was used as experimental animals. The experimental animals were divided into the control and MC treated group and given food and water unlimited. The animals treated with 4mg of MC per kilogram of body weight were sacrificed at 6, 12, 24, 36 and 48 hour after administration. The animals of control groups were administered only water for injection. The liver specimens were fixed in 10% formalin solution at 4℃ and sliced at 14㎛ thickness in a frozen cryostat. The activities of alkaline phosphatase were observed by the Gormori's method and those of ATPase were observed by the Wachstein-Meisel's method for histochemical study. The result are as follows. 1. As the time passed, the activity of alkaline phosphatase activity was decreased in the hepatic lobule. Negative reactions were showed in the central and intermediate zone of the lobule of 36 hours of MC treated group. Trace activities of the enzyme were observed in the whole hepatic lobule of 48 hours of MC treated group. 2. As the time goes by, the activity of adenosine triphosphatase was decreased in the hepatic lobule. Negative reactions were observed in the entire hepatic lobule of 36 hours of MC treated group. Weak activity of the enzyme were observed in the whole hepatic lobule of 48 hours of MC treated group. Consequently, it is suggested that MC causes decrease in activities of hepatic alkaline phosphatase and ATPase due to live damage by cross linking of DNA with the drug in the hepatocyte and destructive change of organelles of the cell.

팔영산 삼림식생과 토양환경

이호준,김종홍,배병호,박문수,전영문,강재구,신정식 建國大學校基礎科學硏究所 1997 理學論集 Vol.22 No.-

전라남도 고흥군 소재 팔영산 (608.6 m) 일대의 삼림군락에 대한 색생조사를 1993년 10월부터 1995년 7월까지 실시하였다. 조사한 자료는 Z-M 방식에 의해 종조성표를 작성하였다. 이에 따라 삼림군락은 신갈나무군락, 졸참나무군락, 굴참나무군락, 서어나무군락, 소나무군락등 5 개 군락으로 분류되었다. 입지와 환경과의 유기적 관계를 조사하기 위해 토양을 분석한 결과, 신갈나무군락은 토양의 비옥도가 높은 산의 능선 부위에 집중 분포하였으며, 굴참나무군락과 소나무군락은 토양의 비옥도가 낮고 비교적 건조한 고도 400 m 이하 지역에 주로 군락을 형성하고 있었다. 또한 졸참나무군락은 고도 300 - 550 m 이내에서 부분적으로 나타났으며, 서어나무군락은 토양의 비옥도가 양호한 고도 300 - 550 m 사이의 골짜기 주변에 국지적으로 분포하였다. 팔영산 삼림식생의 특징은 상록활엽수림이 형성될 수 있는 지역이나 후박나무, 동백나무, 사스레피나무 등의 몇몇 종을 제외하고는 상록활엽수의 분포를 확인할 수 없었으며, 일부 식재림을 제외한 대부분의 지역이 낙엽활엽수와 소나무의 혼효림으로 구성되어 있었다. The phytosociological study forest vegetation on Mt. Palyoung (608.6 m) was investigated from October 1993 to July 1995 in Koheug-Gun, Cheonnam province. According to Z-M method, the floristic composition table of the forest vegetation was divided into five community as follows; Quercus mongolica community, Quercus serrata community, Quercus variabilis community, Pinus densiflora community, Carpinus laxiflora community. The soil was analyzed to investigated the soil conditions and soil fertility. As a result, the organic matters which showing soil fertility were found to be low Quercus variabilis and Pinus densiflora community at comparatively xerophic conditions, below 400 m altitude, while they were increased in Quercus mongolica community on mountain ridge, and that Quercus serrata community was appeared 300-550 m altitude around mountain stream to moderate soilfertility. The forest vegetation of Mt. Palyoung area unconfirmed capable of evergreen broad-leaved forest, but appeared to Machilus thunbergii, Camellia japonica and Eurya japonica species etc. On the other hand, surveyed site's vegetation was distributed mixed forest of deciduous leaved forest and Pinus desiflora, except for some afforestation.

Cis-Dichlorodiammineplatinum이 Mouse 간장의 인산염 분해효소활성에 미치는 영향

유신철,이동준,정호삼,이규식 漢陽大學校環境科學硏究所 1987 環境科學論文集 Vol.8 No.-

cis-Dichlorodiammineplatium(Ⅱ)(cis-DDP)은 최근에 개발된 효과적인 종양화합 요법제인 금속화합물로서 그 작용기작은 아직 불명하나 bifunctional alkylating agent와 유사한 작용 기전을 갖는 것으로 알려져 있다. cis-DDP는 체내에서 가수분해되어 DNA와 반응함을 핵산의 합성을 억제하여 암세포 뿐만 아니라 정상세포에도 영향을 미쳐 출혈성장염, 임파조직과 골수의 저세포증, 신손상, 단백뇨 및 혈뇨등의 부작용을 유발한다. 이에 저자는 cis-DDP가 mouse 간장에서 세포독성으로 작용하여 간실질세포의 변성을 일으키리라 사료되어 간실질세포내의 acid phosphatase, alkaline phosphstase 및 aden-osine triphosphatase의 변동을 조직화학적으로 관찰하였다. 실험동물은 DDY계의 웅성(체중 20g)mouse를 사용하였으며 체중 kg당 6mg의 cis-DDP를 투여한 후, 6시간, 12시간, 24시간 및 48시간 경과후에 희생하여 간조직을 적출하고 10% formalin용액에 고정하고 cryostat로 16㎛두께로 동결절편을 만들었다. 조직화학적인 연구를 위하여 acid phosphatase와 alkaline phosphatase의 활성은 Gomori 방법으로 그리고 adenosine triphosphatase의 활성은 Wachstein-Meisel 방법으로 이들 효소활성을 관찰하여 다음과 같은 결과를 얻었다. 1. Acid phosphatase 활성은 간소엽주변대에서 cis-DDP투여 6시간 경과군의 mouse에서는 약한 양성반응을 12시간 경과군의 mouse에서는 중등도의 양성반응을 48시간 경과군의 mouse에서는 약한 양성반응을 나타내었다. 2. Alkaline phosphatase 활성은 간소엽 주변대에서 cis-DDP투여 12시간, 24시간, 및 48시간 경과군의 mouse에서 중등도의 양성반응을 나타내었다. 3. Adenosine erihosphatase 활성은 간소엽 중심대에서 cis-DDP투여 6시간 경과군의 mouse에서 미약한 양성반응을 나타내었고, 12시간 경과군의 mouse에서는 중등도의 양성반응을 나타내었고, 24시간 및 48시간 경과군의 mouse에서는 각각 중등도 및 강한 양성반응을 나타내었다. 이상의 소견을 종합한바 cis-DDP의 투여로 mouse의 간장 손상을 일으켜 acid phosphstase, alkaline phospatase 및 adenosine triphosphatase 활성은 일시적으로 저하되었다가 회복되는 것으로 사료된다. cis-Dichlorodiammineplatinnm(Ⅱ) (cis-DDP), known as cisplatin, is a metallic compound that has recently been developed as an effective cancer chemotherapeutic agent. The precise mechanism of action of cis-DDP is still unknown. Similarities between the mechanism of action of this drug and bifunctional alkylating agents have been suggested. Upon entering the cell, the platinum compounds will become hydrolyzed, and then they can react with DNA. They act on tumor cells as well as normal cells. Side effects included severe hemorrhagic enterocolitis, severe hypocellularity of the bone marrow and lymphoid tissues, renal lesions, proteinuria and (microscopic henaturia). In this experiment, the author studied the effect of cis-DDP on the mouse liver, histochemically observing the changes in the activities of acid phosphatase, alkaline phosphatase, and adenosine triphosphatase. Male mice (the weight 20g), DDY strain, were used as experimental animals. The experimental animals were sacrificed at 6,12,24 and 48 hours after administration of 6mg of cis-DDP per kilogram of body weight. The specimens obtained from the liver were fixed in 10% formalin solution and sliced at 16㎛ thickness in a frozen cryostat. The activities of acid and alkaline phosphatases were observed by the Gomori's method and the activity of adenosine triphosphatase was observed by the Wachstein Meisel's method for histochemical study. The results are as follows. 1. The acid phosphatase activity was weakly positive at 6 hours and moderately positive at 12 hours and weakly positive at 48 hours after administration in the peripheral zone of the hepatic lobule. 2. The alkaline phosphatase activity was decreased to moderately positive at 12,24 and 48 hours after administration in the peripheral zone of the hepatic lobule. 3. The adenosine triphosphatase activity was trace positive at 6 hours after administration in the central zone of the hepatic lobule and moderately positive at 12 hours after admnistration in the central and peripheral zone of the hepatic lobule. It is suggested that cis-DDP causes changes in activities of acid phosphatase, alkaline phosphatase and adenosine triphophsatase in the liver, due probably to the cytotoxic effect in the parenchymal cells of the liver in mice.

2차 전지용 포장 소재에 관한 연구

라호원,이종대,신장식 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.1

The package of secondary battery is made of material, which stands acid-resistivity because it contains strong acid electrolyte in it. The characteristics of package in secondary battery have been investigated using peel strength test and electrolyte dipping test. When designing the battery cell package, three layer(Nylon66, aluminum, PP + PE copolymer) are used. In peel strength test, experiment results of Crome surface treatment have a superior advantage of adhesives force and anti-leakage characteristics. Also, it is found that polyolefin and polypropylene have good quality in electrolyte dipping test.

木花 收量構成形質의 遺傳分析

崔周浩,李信雨,全鉉植,李喆浩 진주산업대학교 농업기술연구소 2005 農業技術硏究所報 Vol.18 No.-

2001년부터 2003년까지 木花 10개 品種을 二面交配하여 얻은 F_(1), F_(2) 45개 組合을 재료로 生育形質의 交配集團에 따른 遺傳分析한 結果를 요약하면 다음과 같다. 1. 각 형질에 대한 분산분석 결과는 전형질에서 고도의 유의성을 나타내었다. 2. 유전자의 상가적효과의 값은 우성효과의 값보다 높게 나타났고, F_(1)세대의 株當□數에서 가장 높았으며, 百粒重은 F_(2)세대에서 오히려 높아 세대간에는 일정한 경향을 보이지 않았다. 3. 유전력은 섬유장을 제외한 전 형질에서 0,80이상으로 평가되었고, 그 중 섬도는 F_(1)세대에서 0.97로 조사된 형질 중 가장 높았다. The quantitative inheritance of some yield characters in Gosyium spp was carried out by means of a 10×10 diallel cross. This study was genetically analyzed through diallel cross population among 10 different cultivars of cotton (Gosyium spp) at an experimental field in Jinju National University. According to research from April 2001 to December 2003, the results of genetic analysis were summarized as follow: 1. The high significance was observed from the genetic variance analysis in the all examined characters. 2. In the component of genetic variance analysis, additive effects were relatively higher than dominant effects. The number of boll in F_(1) generation was higher than F_(2), whereas no significant difference was seen in the dominance degree and gene arrangement between F_(1) and F_(2) generations because 100 seeds weight in F_(2) generation was much higher F_(1). 3. The progeny of F_(1) heritability was found to exceed a suggested minimum of 0.80 excepting fiber length. The heritability of F_(1) in fiber fineness was 0.97 relatively higher than other all examined characters.

Interleukin-2가 Mouse 자궁내막의 Acid Phosphatase 활성에 미치는 영향

김원규,신규철,정호삼,이규식 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2

Interleukin-2, a kind of lymphokine produced by the T-lymphocyte stimulated by antigen or mitogen, has been studied intensively with the development of immuno-oncology and chemoimmunotherapy of the malignancy. Interleukin-2 is known to exert on antitumor effect by stimulating the proliferation of helper and cytotoxic T lymphocytes, augmenting the cytolytic activities of the cytotoxic T lymphocyte and differentiating a precursor cell into a lymphokine-activated killer cell in vivo. Though interleukin-2 suppresses the metastasis of the malignant tumor into the adjacent organs, it develops many side effects including fever, chills, malaise, mental confusion, diarrhea, vomiting and oliguria. Interleukin-2 also causes pulmonary, hepatic and renal toxicities and, especially, increase of capillary permeability with weight gain, pulmonary edema and ascites. In this experiment, the author studied the effect of interleukin-2 on the mouse endometrium, observing histochemically the changes in the activity of acid phosphatase following the estrous cycle. Female, DDY strain, weighing 20gm and exhibiting normal estrous cycle, were used as experimental animals. The estrous cycle was recognized by the vaginal smear. The experimental animals were treated with 2 million units of interleukin-2/0.2ml per Kg of body weight and with 0.2ml of normal saline per Kg of body weight in the control groups for 5 days. And animals were sacrificed 5th day after administration of interleukin-2. The specimens obtained from the corpus uteri were fixed in 10% neutral buffered formalin solution for 24 hours at 4℃ and sliced at 14μm thickness in a frozen cryostat. The activity of acid phosphatase was observed by the Gomori's method for histochemical study. The result obtained was as follows: The acid phosphatase activity was increased at estrus and decreased at diestrus in interleukin-2 treated group. There was, however, no marked differences in acid phosphatase activity of the proestrus and metestrus between control and interleukin-2 treated groups. Consequently, it was suggested that interleukin-2 develop the changes of the acid phosphatase of the endometrium of the mouse.