Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

성인에서 발생한 설사 동반성 용혈성 요독 증후군 3례

김진호,장광열,김구,배성진,김성은,김기현,최필선 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.2

초음파 가이드를 통한 맥락막상강출혈의 치료

이기헌,유재호,김태원,이상준,김신동 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

Purpose : Intraoperative suprachoroidal hemorrhage is one of the most serious complications. We report a case of suprachoroidal hemorrhage during phacoemulsification surgery, was managed properly. Methods : Suprachoroidal hemorrhage occurred during phacoemulsificaiton in other hospital. The patient was referred to our department immediately after closing the surgical wound. On initial examination, iris and vitreous were prolapsed through surgical wound. Ciliary body was closely place to anterior chamber, and retina was not visible. Results : Prolapased ocular tissue were reduced by emergency operation. We controlled the patient’s intraocular pressure and inflammation. Ultrasonography was repeatedly performed to check the degree of liquefaction of suprachoroidal hemorrhage. After 9 days of suprachoroidal hemorrhage onset, vitrectomy and drainage through sclera was performed. Postoperative state was stable with successful removal of suprachoroidal hemorrhage. After 2 weeks of secondary operation, retinal detachment has occurred on nasal retinal area, so we performed scleral buckling and endolaser photocoagulation. After 6 months, retina was attached well and intraocular pressure was in normal range. Conclusion : In suprachoroidal hemorrhage, drainage of suprachoroidal hemorrhage after liquefaction of thrombi is effective. After successful drainage, postoperative complications may accompany such as retinal detachment or elevation of intraocular pressure. 목적 : 술중 발생하는 맥락막상 출혈은 매우 심각한 합병증이다. 저자는 수정체 유화술 중에 발생한 맥락막상 출혈을 적절히 대처한 증례를 보고하는 바이다. 대상과 방법 : 수정체 유화술 중 발생한 맥락막상강 출혈환자가 수술창을 봉합 후 즉시 본원으로 전원되었다. 초진시 홍채와 유리체가 수술창을 통해 탈출되어 있었으며, 전방을 통해 모양체가 확인되었으나, 망막은 보이지 않았다. 탈출된 안구내 조직을 응급수술을 통해 복원 후 환자의 안압과 염증을 조절하였다. 초음파를 반복적으로 실시하여 맥락막상 출혈의 액화 정도를 확인하였으며, 출혈 9일 후 유리체 절제술과 공막을 통한 배액술을 시행하였다. 결과 : 맥락막상 출혈이 성공적으로 제거되었으며 술 후 상태는 안정적이었다. 수술 2주 후 비측 망막 박리가 발생하여 공막돌륭술과 안구내 삽관 레이저술을 시행하였다. 6개월 후 망막은 잘 붙어있었으며, 안압은 정상 범위를 유지하였다. 결론 : 맥락막상 출혈의 경우 출혈의 배액 시기를 결정하는 것이 어렵지만, 규칙적인 초음파를 통해 배액술의 시기를 결정할 수 있다. 또한 맥락막상강 출혈의 성공적인 배액 이후에도 망막박리 및 안압상승과 같은 합병증의 동반에 대해 유념해야한다.

금속재료의 기계적 물성과 피로한도의 상관 관계

윤웅기 ; 조백희 ; 이성호 김천대학교 2000 김천대학교 논문집 Vol.21 No.-

The dynamic fatigue-life equation is applied to the uniaxial tensile test. On this study, we show that the fatigue limit values can be extracted from the tensile testing data. Consequently, those experi-ments can be correlated and the fatigue limit can be found by the tensile test. The theory is verified by comparing the theoretical fatigue limit from tensile test to experimental fatigue limit from fatigue test. The results can be used to find the reliable fatigue limit by tensile test.

茵蔯淸肝場의 B型 및 C型 간염 바이러스에 대한 抗 바이러스 效果

김수성,김기열,윤철호,서운교,김종대,정지천 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

우리나라와 같이 B형 간염 바이러스의 유병율이 높은 지역에서는 B형 간염 바이러스 뿐만 아니라 HCV에 대한 항 바이러스 효과도 동시에 시행함이 유용할 것으로 사료되었다. 본 연구에서는 茵蔯淸肝湯을 이용하여 B형 간염 바이러스에 대한 抗 바이러스 효과를 PCR을 이용하여 HBV의 DNA를 검색하였다. 茵蔯淸肝湯이 처리된 처리군의 경우 대조군에 비하여 농도 의존적으로 B형 간염 바이러스의 표면항원 생산을 억제하였고, PCR을 이용하여 증폭한 경우에도 증폭된 DNA의 양은 감소하였다. 또한 C형 간염 바이러스에 대한 抗 바이러스효과를 검색하기 위하여 복제에 필수적인 단백질인 HCV helicase의 ATPase에 대한 활성 억제 효과를 조사하였다. 茵蔯淸肝湯이 HCV helicase의 ATPase 활성을 억제하는 효과가 있음을 확인한 후 dose-respond에 따른 실험을 수행하여 HCV helicase의 ATPase 활성을 억제함으로써 탈인산화 되지 않은 ATP의 양을 이용하여 PSL로 분석한 결과 茵蔯淸肝湯이 HCV helicase의 ATPase 기능에 대한 억제효과가 우수함이 확인되었다. 이상의 결과로 보아 茵蔯淸肝湯이 B형 및 C형 간염 치료에 동시에 응용될 수 있을것으로 사료된다. For the purpose of investigate the inhibition effects of Injinchunggantang on Hepatitis and C Virus. The inhibition experiments were as follows : 1. The inhibition effects of Hepatitis B surface antigen production was remarkably increased in the pretreated group of Injinchunggantang compared with control group. 2. Amplified PCR products of HBV-DNA in culture media was considerably decreased in the pretreated group of Injinchunggantang compared with control group. 3. Results of ATP quantitation and ATPase inhibition percent was increased pretreated group of Injinchunggantang. Also ATPase activity of HCV helicase inhibition assay was increased with a dose dependent manner. These results may suggest that Injinchunggantang will have the inhibition effects of Hepatitis B and C Virus.

악성림프종 환자에서 복합화학요법으로 유발된 백혈구 감소증에 대한 인형 재조합 과립구 집락형성-촉진인자(rhG-CSF: Neutrogin)의 효과

박성규,전진우,기신영,유병우,김홍수,백승호,원종호,홍대식,박희숙 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Clinical effects of rhG-CSF were investigated in 17 patients receiving chemotherapy for non-Hodgkin's lymphoma. Patients were given G-CSF 2㎍/㎏/day after 2nd cycle chemotherapy for least 14 consecutive days, and clinical and laboratory results were compared to results of the first cycle. Treatment with G-CSF resulted in increase of mean WBC count(952.9±152.2㎜³vs 1882.4±350.4/㎜³) and neutrophil count(212.9±53.0/㎜³vs 841.5±238.0/㎜³), reduced the duration of leukocytopenic days(12.4±1.3 days vs 5.5±1.0 days, WBC count less than 4000/㎜³) and neutropenic days(4.5±0.9 days vs 2.3±0.7 days, neutrophil count less than 500/㎜³), and reduced the duration of neutropenic fever(5.8±1.1 days vs 0.9±0.5 days). The side effects of G-CSF were not so significant. These results demonstrated that G-CSF is safe and useful for the treatment of neutropenia induced by anticancer chemotherapy of non-Hodgkin's lymphoma and reducing the incidence of febrile periods and duration of antibiotic administration.

류머티스 관절염과 골관절염 환자에서 Transforming grwoth factor β의 발현 양상

김채기,윤원찬,송용호,김상경,최정윤 Official Journal of the Korean society for immunol 2001 Immune Network Vol.1 No.3

공동주택 바닥충격음 차폐성능평가 실태에 관한 연구

윤웅기 ; 이성호 ; 조백희 김천대학교 2002 김천대학교 논문집 Vol.23 No.-

Now, Resident in apartment is over 50% in our country.

10~12세 남자아동의 신체조성과 혈중지질성분 비교

김정기,박승한,변재철,김규호,조영주,허용 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to compared blood lipids level in male childrens. Aged are 10-12 years. There are participated in obese, athletes, and general male childrens. We evaluated the underwater weighing body compositions, plasma triglyceride (TG), serum total cholesterol, high density lipoprotein (HDL) and low density lipoprotein (LDL) cholesterol levels. in this study, the underwater weight and body density were highest in athlete group compared other groups. The percent body at was higher in obese children than other groups. Furthermore, plasma TG level was higher in obese children group than the other two groups. Serum TC, LDL-C concentration were higher in obese children group than the other two groups. Also, Serum HDL-C level was high in obese children group compared other two groups.

Cisapride가 심근의 ATP-sensitive K 통로에 미치는 영향

문성기,인병현,김원호,고재기 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.1