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Taxol 합성에 관한 연구 I :O-(tert-Butyldiphenylsilyl)-cis-5-methyl-4-hepten-6-yn-1-ol의 합성
박두천,주우홍,김형국,신동수 國立昌原大學校 基礎科學硏究所 1994 基礎科學硏究所論文集 Vol.6 No.-
O-(tert-Butyldiphenylsilyl)-cis-5-methyl-4-hepten-6-yn-1-ol, an intermediate in the synthesis of taxol, was efficiently synthesized from 3-methyl-2-penten-4-yn-1-ol by sequential alcohol/bromide interchange [NBS, PPh₃, CH₂Cl₂-30.℃, 82%], displacement with the lithium salt of tert-butylacetate [LDA, THF, -78 to 23℃, 84%], LAH reduction, and silylation [tBuPhSiCl, imidazole, CH₂Cl₂, 45℃, 88%]
스칼라 曲率 (H ≠0)을 갖는 再歸 Finsler 空間에 關하여
朴弘緖,金秉斗 嶺南大學校 基礎科學硏究所 1990 基礎科學硏究 Vol.10 No.-
Generally, if a space F?? is a recurrent Finsler space, then the deviation tensor and the projective deviation tensor are also recurrent. But the converse doesn't hold good. In the present paper, we shall study the converse condition, that is, H? recurrent space is a recurrent Finsler space if a certain condition (A) holds.
자가 Fibrin Glue와 연골세포를 이용한 연골조직 공학 기법에 관한 연구
홍성표,범진식,박재경,김진영,이두형 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3
Neomorphogenesis of cartilage using chondrocyte-polymer constructs is a potential source for development of cartilage reconstruction. Current tissue engineering techniques of neocartilage rely on in vivo implantation of polymer-chondrocyte constructs. The purpose of this study was to find a way to bioengineer cartilage in vitro by entrapping chondrocytes in a molded autologous fibrin glue. Chondrocytes isolated from the cartilage of rabbit joints were combined with fibrinogen extracted by a single cryoprecipitation of autologous plasma, and they were then polymerized with thrombin to create a fibrin glue with a final cell density of 2.5x10?? cells/ml. The collagen for a control study was used as a polymer. The polymer-chondrocyte constructs were cultured for 4 weeks and the fibrin-chondrocyte constructs molded in the shape of a human ear were cultured for 6 weeks in vitro. Morphometric, histochemical, and histomorphometric analysis including glycosaminoglycan quantitation confirmed the following results: 1) Highly-concentrated autologous fibrinogen was easily extracted by a single cryoprecipition of autologous olasma. 2) The fibrin-chondrocyte constructs demonstrated the presence of actively proliferating chondrocytes with the production of cartilaginous matrix(collagen and glycosaminoglycan) at 1 week after culture, as well as gross and histologic evidence similar to those of normal cartilage at 3∼4 weeks after culture. 3) The collagen-chondrocyte constructs demonstrated lower degrees of hardness and transparency, as well as a lower density of cells and glycosaminoglycan during the culture period. 4) Neocartilage generated from fibrin-chondrocyte constructs in the shape of a human ear nearly retained their original configuration and size without degeneration for 6 weeks of culture in vitro. This study demonstrated a novel method for bioengineering the molded cartilage in vitro using autologous fibrin glue as a matrix scaffold. The generated cartilage showed gross and histologic evidence similar to those of normal cartilage, retaining the original gross dimension. With further refinement, this may be a new application of tissue engineering for the reconstruction of cartilage.
박재선,박두병,이길홍 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.4
The present study was to examine the relationship between psychiatric inpatients' occuputions and their anxiety level. The sampling subjects were randomly selected by 3 stage probability proportional culster extraction method among psychiatric who were admitted to the psychiatric clinics and hospitals nationwide. Four hundred and twenty eight subjects including 249 males and 179 females were undergone by questionnaire survey while measurement of anxiety was done by using Zung's self-rating anxiety scale. Collected data were processed by IBM PC AT computer using SPSS program and statistically analyzed by chi-square test and analysis of variance. 1) It was revealed that these psychiatric inpatients showed relatively higher rates of the unemployed (34.6%), single(43.2%), and divorce(5.1%). 2) Patients with higher classes of occupation such as administrative, managerial or clerical workers showed higher rate of divorce. 3) In the occupational distribution of psychiatric disorders, psychotic disorders were more prevalent among high or low occupational classes, neurotic disorders more prevalent among middle occupational classes, while psychoactive substance use disorder more prevalent among farmers and sales workers. 4) Overall the mean anxiety score of the whole subjects is 39.28 ±14.67. The mean anxiety score (39.71±14.89) of the pschiatric inpatients with job is higher than that (36.59±14.20) of the unemployed. 5) In the anxiety tendency according to occupational classification, the unemployed showed less anxiety compared with the employed. Patients with middle occupational classes such as fishing workers and sales workers showed higher levels of the cardiovascular, musculoskeletal, respiratory and skin system anxiety, while patients with higher or lower occupational classes showed lower anxiety. In the relationship between occupational classes and level of anxiety, it showed profiles of inverted V curve. 6) The lower occupational classes such as sales workers showed higher anxiety level, while the higher occupational classes such as clerical workers showed lowest one. Particularly the fishing group exposed in the higher stress situation showed the highest level of anxiety.
Most Probable Number 방법을 이용하여 측정한 중랑천 河床土壤의 혐기성 세균의 수와 수질과의 상관 관계
박두현,김병홍,임시근,최영효 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3
중랑천의 하상 토양의 혐기성 호흡세균의 분포를 MPN법으로 조사하였다. 철이온환원세균을 조사하기 위해 배양액 중의 Fe(Ⅱ)을 확인하는 변형된 oxalate-ferrozine 법을 개발하였으며, 황산염환원세균(SRB)과 메탄생산세균을 각각 FeS 침전과 head space의 CH_4를 검출하는 방법을 채택하여 중랑천 지천과 본천의 혐기성 미생물 분포를 조사한 결과 오염 정도가 상대적으로 높은 본천에서 철이온환원세균이 10^7~10^8 cells/g 정도로 조사되었으며 지천의 경우 10^7 cells/g이었다. 우이천 상류를 제외한 모든 조사 지점에서 SRB와 methanogen 이 10^3 cells/g이었다. 이 결과는 분류 하수관의 설치로 유기물의 유입이 낮아진 조건에서도 하상의 침전층에서 유래하는 유기물을 이용하여 철이온환원세균이 생장할 수 있으며, SRB와 methanogen의 생장은 철이온환원세균에 의해 방해를 받는다는 것을 나타낸다. Sediments collected from the Jungnang-cheon and its tributaries were used to enumerate anaerobic bacteria by most probable number (MPN) methods. A simple method was developed to detect ferrous ion in the culture fluid in order to count the number of iron ion reducers, and sulfate-reducing bacteria (SRB) and methanogens were detected by the presence of FeS precipitate in the culture or methane in the head space, respectively. The numbers of iron reducer was in the range of 10^7~10^8/g in the sediment of the stream containing higher organic content than the tributaries. The sediments of tributaries were analyzed to contain iron reducers less than 10^7 cells/g. With one exception the numbers of SRB and methanogens were less than 10^3 cells/g in the sediment. From these results it is concluded that organics in the sediment support the growth of iron reducers, which out-compete SRB and methanogens.
퍼지추론기법을 이용한 2-자유도 비선형 모형 헬리콥터의 자세 제어
朴斗煥,李俊柝,河泓坤 東義大學校産業技術開發硏究所 1998 産業技術硏究誌 Vol.12 No.-
The helicopter system is non-linear and complex. Futhermore, because of absence of an accurate mathematical model, it is difficult accurately to control its attitude. Therefore, we propose a fuzzy control technique to control efficiently its elevation angle and azimuth one. This controller is on the basis of expert's knowledges and his experiences. The simulation results using MATLAB are introduced.