http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Whole-genome resequencing analysis of 20 Micro-pigs
Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3
Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.
일부 고지혈증 폐경 여성의 Isoflavone 공급에 따른 혈중지질 변화에 관한 연구
이다홍,승정자,이행신,김미현,서유리 대한지역사회영양학회 2001 대한지역사회영양학회지 Vol.6 No.1
Intake of soy protein may decrease the risk factors associated with cardiovascular disease in postmenopausal women. This study was designed to investigate the effects of isoflavone supplementation on serum lipids in 16 hyperlipidemic postmenopausal women. For this purpose, an intervention study was conducted for 12 weeks. Subjects were healthy, free-living women consuming habitual diets with 0.3 g/d of isoflavone. Food and nutrient intake was obtained by 24-hr recall method and anthropometric measurements were made. Systolic and diastolic blood pressure, total serum cholesterol, HDL-cholesterol and LDL-cholesterol were determined before and after the isoflavone supplementation. The results were summarized as follows. The average age, height, weight and BMI of the subjects were 65.3 years, 151.4 cm, 62.2 kg and 27.1, respectively. The systolic blood pressure and diastolic blood pressure were not reduced significantly with isoflavone supplementation. Total cholesterol (p < 0.001), HDL-C (p < 0.05), and LDL-C (p < 0.01) were significantly increased after isoflavone concentration. In conclusion, isoflavone supplementation was not effective to modify risk factors for cardiovascular disease.
곽승준(Seung Jun Kwack),김순선(Soon Sun Kim),최요우(Yo Woo Choi),이규식(Gyu Seek Rhee),손경희(Kyung Hee Sohn),이이다(Rhee Da Lee),채수영(Soo Young Chae),정용현(Yong-Hyun Chung1),유일재(Il Je Yu1),박귀례(Kui Lea Park) 한국독성학회 2004 Toxicological Research Vol.20 No.2
3-Monochloro-1,2-propanediol(3-MCPD) is a toxic compound, often present in different foods containing acid hydrolyzed(AH) protein, like seasonings and savory food products. The purpose of the present study was to investigate the effects of 3-MCPD on male fertility, sperm and testosterone secretion. In vivo male fertility test was performed for observing the adverse effects of 3-MCPD on the function of male reproductive system and pregnancy outcome. 0.01, 0.05, 0.25, 1 and 5 mg/kg b.w. of 3-MCPD was given daily by gavage to groups of 15 adult male SD rats for 4 weeks. At the end of pre-treatment period, males were mated overnight with normal females. Following morning, males demonstrating successful induction of pregnancy were sacrificed on that day to assess sperm parameters and histopathology of reproductive organs. The resulting pregnant females were sacrificed on day 20 of gestation to evaluate pregnancy outcome. As a result, four-week paternal administration with 3-MCPD resulted in adverse effects on male fertility and pregnancy outcome without remarkable histopathological changes in testes and epididymides; sperm motility, copulation index and fertility index were markedly decreased in the treated group and numbers of live fetuses showed steep dose-response curves. Also, spermatogenesis was investigated in this experiment. However, no<br/> effect was observed on production of sperm in testes treated with 3-MCPD for 4 weeks. Hormone assay was performed for observing the effects of 3-MCPD on testosterone and luteinizing hormone (LH) in blood and testes of male SD rats and cultured primary Leydig cell. In result, significant changes of related hormones did not observed by treatment of 3-MCPD. These results indicated that paternal treatment with 3-MCPD induced spermatotoxic effect, which caused an antifertility on male.
( Hyun Soo Chung ),( Da Hyun Jung ),( Jun Chul Park ),( Sung Kwan Shin ),( Sang Kil Lee ),( Yong Chan Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background and study aims: During emergency endoscopy for gastrointestinal bleeding, sometimes proper endoscopic treatment is hampered by poor cooperation of patients. The aim of this study was to evaluate the safety and effi cacy of propofol sedation during emergency upper endoscopy for patients with gastrointestinal bleeding. Patients and Methods: From March 2013 to June 2014, a total of 112 patients with acute upper gastrointestinal bleeding underwent propofol sedation. Patients who were hemodynamically unstable or received inotropics were excluded. Patients without sedation were matched for age, gender, and ASA class. Treatment outcome, procedure time, patient`s and endoscopist`s satisfaction, and adverse effect were investigated. Results: The mean age was 64.5 years (m:f = 1.9:1) and 24 patients were classifi ed in ASA class II, 69 in III and 19 in IV . There was no signifi cant difference between groups in success rate of endoscopic hemostasis (95.6% (107/112) vs 94.7% (106/112). Propofol group had shorter procedure time (36.3 min (7-65min) vs 42.5min (7-72), p=0.04) and higher patients’ and endoscopists` satisfaction (p=0.041 and p=0.022). Frequency of hypotension, bradycardia and hypoxemia was 4.5% (5/112), 1.8% (2/112) and 5.4% (6/112) in propofol group. However, there were no needs for endotracheal intubation or mask ventilation and there was no sedation-related death or serious complications. Conclusion: Gastroenterologist-directed propofol sedation during emergency upper gastrointestinal endoscopy is safe under strict monitoring and is associated with shorter procedure time, and higher satisfaction both in endoscopists and in patients.
( Da Hyun Jung ),( Yong Chan Lee ),( Jie-hyun Kim ),( Hyunsoo Chung ),( Jun Chul Park ),( Sung Kwan Shin ),( Sang Kil Lee ),( Hyoung-il Kim ),( Woo Jin Hyung ),( Sung Hoon Noh ) 대한간학회 2017 Gut and Liver Vol.11 No.5
Background/Aims: Few studies have evaluated the effect of Helicobacter pylori infection on the prognosis of patients diagnosed with gastric cancer (GC) after curative surgery. We investigated the association between the H. pylori infection status and clinical outcome after surgery. Methods: We as-sessed the H. pylori status of 314 patients who underwent curative resection for GC. The H. pylori status was examined using a rapid urease test 2 months after resection. Patients were followed for 10 years after surgery. Results: An H. pylori infection was observed in 128 of 314 patients. The median follow-up period was 93.5 months. A Kaplan-Meier analysis indicated that patients with H. pylori had a higher cumula-tive survival rate than those who were negative for H. pylori. Patients with stage II cancer who tested negative for H. py-lori were associated with a poor outcome. In a multivariate analysis, H. pylori-negative status was a significant indepen-dent prognostic factor for poor overall survival. Conclusions: Having a negative H. pylori infection status seems to indicate poor prognosis for patients with GC who have undergone curative resection. Further prospective controlled studies are needed to evaluate the mechanism by which H. pylori af-fects GC patients after curative surgery in Korea. (Gut Liver 2017;11:635-641)
Kim, Da Hyun,Chung, Woo-Chang,Chun, Su-hyun,Han, Ji Ho,Song, Moon Jung,Lee, Kwang-Won Elsevier 2018 Journal of dairy science Vol.101 No.12
<P><B>ABSTRACT</B></P> <P>Influenza A virus (IAV) infection is a global public health concern. It causes respiratory diseases ranging from mild illness to fatal disease. Natural killer (NK) cells are an innate immune component that kill infected cells and secrete cytokines to modulate the adaptive immune system; they constitute the first-line defense and play important roles in controlling IAV infection. This study evaluated the effect of daily administration of heat-treated <I>Lactobacillus plantarum</I> nF1-fortified yogurt on immunity and protection against IAV infection. Mice administered with heat-treated <I>L. plantarum</I> nF1-fortified yogurt showed elevated NK cell-related cytokine expression levels. Daily administration of the <I>L. plantarum</I> nF1-fortified yogurt before IAV infection also enhanced splenic NK activity, lung inflammatory cytokine responses, and survival rate. Thus, daily administration of nF1-fortified yogurt enhances host immunity and helps prevent IAV infection.</P>