In vivo study for the hemostatic efficacy and foreign body reaction of a new powder-type polysaccharide hemostatic agent

Yoonhyeong Byun,Eun Jin Kim,Areum Lee,Young-Ah Suh,Hee Ju Sohn,Jung Min Lee,Jae Seung Kang,Yoo Jin Choi,Youngmin Han,Hongbeom Kim,Wooil Kwon,Jin-Young Jang 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.102 No.2

Purpose: Various hemostatic agents have been introduced in therapy as postoperative bleeding is a poor prognostic factor for postoperative outcomes. These products can be divided into those that directly promote the hemostatic cascade and those that physically form a barrier by absorbing blood. The latter, powder-type hemostatic agents have the advantages of being inexpensive and more absorbable with less foreign body reactions (FBRs) and are applicable to a relatively wide area. This study was conducted to verify the safety and efficacy of a newly invented polysaccharide product (OOZFIX, Theracion Biomedical), which improves blood absorption and hemostatic effects. Methods: Two separate animal experiments were performed. The first evaluated FBRs histologically at 3 days, 2 weeks, and 4 weeks, after implantation of OOZFIX in rats, and the second compared hemostatic performance of OOZFIX and Arista AH (Bard) in the porcine liver punch biopsy model. Results: We found minimal FBRs in the 3-day group and no reactions in both the 2-week and 4-week groups after implantation of hemostatic agents. The time to hemostasis of OOZFIX was not significantly different from that of Arista AH (median [interquartile range]: 9 [6–10] minutes vs. 8 [6–10] minutes, respectively; P = 0.522). When comparing the serial bleeding grade tendency, there was no statistical difference between OOZFIX and Arista AH (P = 0.656). Conclusion: OOZFIX caused a minimal FBR that disappeared within 2 weeks in vivo, and its hemostatic performance was comparable with that of an existing agent, Arista AH. Further clinical studies are required in the future.

기관지 방선균증의 임상 1례

이은영,장태원,정만홍,옥철호,이규원,손창배,이지숙,신은경,서정아,백종현,김영옥 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Actinomycosis is a chronic suppurative infection, whitch infiltrates mucosa-associated tissues. Thoracic, pelvic and cervicofacial infections of actinomyces are not uncommon, but endobronchial actinomycosis is rarely reported. We report a case of a 29 year old man who presented with a recurrent pneumonia and chronic cough. Fiberoptic bronchoscopy revealed occlusion of the right. lower medio-basal segment bronchus with exophytic endobronchial mass. The diagnosis was confirmed histologically by demonstration of sulfur granules in the bronchoscopic biopsy of the mass. Intravenous administration of amoxacillin and clindamycin followed by oral amoxacillin and clindamycin therapy resulted in complete recovery.

콩 및 당의 종류의 달리한 매실 콩아이스크림의 품질특성

이숙영,김아영,유지영,이중기,이향섭 중앙대학교 생활과학연구소 2003 생활과학논집 Vol.17 No.-

The purpose of this experiment was to compare the quality characteristics of soy ice creams prepared with soy protein isolate(SPI) or Jinpum-soybean, and different kinds of sweeteners of maltitol and sucrose, xylitol+sucrose, or malitiol. The viscosity of the ice cream mix containing xylitol+sucrose appeared the highest, and that from Jinpum-soybean was higher than that from SPI. The overrun of soy ice creams containing xylitol+sucrose was the highest, and the next was the samples containing maltitol+sucrose. The overrun values of Jinpum-soybean samples appeared higher than those of SPI samples. As for the case of the SPI samples, the melt-down percent was the highest in the sample of maltitol addition, and the next were maltitol+sucrose addition, xylitol+sucrose addition in order. The melt-down percent of Jinpum-soybean was the highest in the sample of maltitol+sucrose addition, and the next were maltitol addition, xylitol+sucrose addition in order. The higher the viscosity of samples, the lower the melt-down percent. In sensory characteristics, the beany flavor, flavor, mouthfeel, overall quality of Jinpum-soybean samples were evaluated better than those of SPI in general. The samples containing xylitol+sucrose evaluated better in beany flavor, mouthfeel, icy feel, and overall quality. The quality characteristics of the samples prepared with Jinpum-soybeans and xylitol+sucrose were evaluated the best.

Light-induced isomerization dynamics of a cyanine dye in the modulus-controlled regime

Jee, Ah-Young,Park, Seohyun,Lee, Minyung Royal Society of Chemistry 2011 Physical chemistry chemical physics Vol.13 No.33

<P>The <I>trans</I>–<I>cis</I> isomerization of an excited molecule converts light energy into mechanical motion, which interacts cooperatively with its surroundings. To understand such a photodynamic process in solids, we investigated the internal twisting motion of 1,1′-diethyl-2,2′-cyanine iodide (DCI) in a series of poly(alkyl methacrylate) (PAMA) polymers by measuring the Young's moduli of the polymers with atomic force microscopy nanoindentation and the fluorescence lifetimes of the dye with time-correlated single photon counting. We found that the isomerization rate constant obtained from the average lifetime correlated well with the mechanical property of the matrix. Our results show that the light-induced molecular motion lies in the modulus-controlled regime in which the polymer matrix not only provides a rigid environment for the dynamics of the molecules but also participates actively in the motion. The concept of elastic modulus may be applicable to molecular rotor dynamics in any synthetic polymer and, in principle, can be extended to biopolymers such as proteins or DNA.</P> <P>Graphic Abstract</P><P>Twisting motion of a photoexcited cyanine dye in poly(alkyl methacrylate) polymers correlated well with the Young's modulus of the medium. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cp20835g'> </P>

한국비서자격시험의 개선점과 비교연구

남자현,이미정,이미영,이아영,정해금,최유정,이미숙,전인선,정혜림 이화여자대학교 법정대학 비서학과 1997 비서학연구 Vol.- No.17

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

Predicting postoperative total calcium requirements after parathyroidectomy in secondary hyperparathyroidism

( Byung Heon Kang ),( Soon Young Hwang ),( Jeong Yeop Kim ),( Yu Ah Hong ),( Mi Yeon Jung ),( Eun Ah Lee ),( Ji Eun Lee3 ),( Jae Bok Lee ),( Gang Jee Ko ),( Heui Jung Pyo ),( Young Joo Kwon ) 대한내과학회 2015 The Korean Journal of Internal Medicine Vol.30 No.6

Background/Aims: To prevent hypocalcemia after parathyroidectomy (PTX), parenteral calcium is required in addition to oral calcitriol and calcium. After switching to oral calcium, patients can be discharged from the hospital. The aim of this study was to analyze the clinical characteristics and outcomes of PTX performed at a single Korean center and to investigate the associated laboratory factors used to analyze the total amount of postoperative calcium required. Methods: We enrolled 91 hemodialysis patients undergoing PTX from November 2003 to December 2011. We collected clinical and laboratory data preoperatively, 12 and 48 hours postoperatively, at discharge, and 3 and 6 months postoperatively. Results: In total, 59 patients underwent PTX with autotransplantation (AT), 6 underwent total PTX without AT, 11 underwent subtotal PTX, and 15 underwent limited PTX. Total PTX without AT showed the lowest recurrence rate. At all postoperative time points, the mean levels of serum calcium, phosphorus, and intact parathyroid hormone (iPTH) decreased significantly, compared with preoperative levels; however, alkaline phosphatase (ALP) increased significantly from 48 hours postoperatively to discharge (p < 0.001). On multiple linear regression analysis, the total amount of injected calcium during hospitalization showed a significant correlation with preoperative ALP (p < 0.001), preoperative iPTH (p = 0.037), and Δphosphorus at 48 hours (p < 0.001). We developed an equation for estimating the total calcium requirement after PTX. Conclusions: Preoperative ALP, preoperative iPTH, and Δphosphorus at 48 hours may be significant factors in estimating the postoperative calcium requirement. The formula for postoperative calcium requirement after PTX may help to predict the duration of postoperative hospitalization.

Amino-Functionalized Mesoporous Silica Particles for Ocular Delivery of Brimonidine

Kim, Se-Na,Ko, Song Ah,Park, Chun Gwon,Lee, Seung Ho,Huh, Beom Kang,Park, Yoh Han,Kim, Young Kook,Ha, Ahnul,Park, Ki Ho,Choy, Young Bin American Chemical Society 2018 MOLECULAR PHARMACEUTICS Vol.15 No.8

<P>To treat glaucoma, conventional eye drops are often prescribed. However, the eye drops have limited effectiveness as a result of low drug bioavailability due to their rapid clearance from the preocular space. To resolve this, we proposed amino-functionalized mesoporous silica (AMS) particles as delivery carriers of the glaucoma drug, brimonidine. Because of the presence of mesopores, brimonidine (BMD) could be encapsulated in the AMS with a loading amount of 41.73 μg/mg (i.e., drug loading capacity of about 4.17%) to give the BMD-AMS, which could release the drug in a sustained manner over 8 h. BMD-AMS was also shown to be mucoadhesive due to the presence of both hydroxyl and amino groups in the surface, allowing for formation of hydrogen bonds and an ionic complex with the mucin, respectively. Therefore, when topically administered to rabbit eyes in vivo, BMD-AMS could reside in the preocular space for up to 12 h because of its adherence to the mucous layer. To assess in vivo efficacy, we examined the variance in intraocular pressure (IOP) and brimonidine concentration in the aqueous humor (AH) after applying BMD-AMS to the eye, which was compared with that induced by Alphagan P, the marketed brimonidine eye drops. For BMD-AMS, the duration in the decrease in IOP and the area under the drug concentration in the AH-time curve (AUC) were 12 h and 2.68 μg·h/mL, respectively, which were about twice as large as those obtained with Alphagan P; this finding indicated enhanced ocular bioavailability of brimonidine with BMD-AMS.</P> [FIG OMISSION]</BR>

Estradiol에 의한 흰쥐-자궁의 수축 기능과 Polyamine 대사의 변동에 관한 연구

이명아,고재홍,이영재,신경호,전보권,천연숙 고려대학교 의과대학 1995 고려대 의대 잡지 Vol.32 No.3

The polyamines have been postulated to be essential in most processes associated with both hypertrophy and hyperplasia. The effects of estrogen on uterine contractility vary with animal species, and most reports have shown that in vitro contractility of rat uterus is decreased after estrogen treatment, but estrogen increases polyamine contents in castrated rat uterus. This study was carried out to investigate whether the regulation of polyamine metabolism may be instrumental in the contractile response of castrated nonpregnant uterus of female SD rats to electrical field stimulation (40 V, 100 μsec : EFS). Estradiol (E2) increased uterine contents of putrescine (PT), spermidine (SPD), and spermine (SPM). These E2 effects were inhibited by tamoxifen or DFMO, but E2-incuced increase of uterine SPM content was enhanced by aminoguanidine. E2 decreased the amplitude of force developed in the rhythmic spontaneous contraction of rat uterus, but it exaggerated the increase of uterine contraction force in response to EFS. The E2-dependent exaggeration was inhibited by tamoxifen, DFMO, or indomethacin, but was significantly enhanced by aminoguanidine. The synergism between E2 and aminoguanidine on uterine contractility was little affected by indomethacin. These results suggest that E2-dependent increase of uterine contractility may be ascribed partly to increase of uterus polyamines.

열공성 뇌경색과 뇌내출혈의 위험인자 비교

이장준,이현아,최종환,이형,임정근,이상도,박영춘 계명대학교 의과학연구소 2000 계명의대학술지 Vol.19 No.2

Cerebral small vessel disease is the most important cause of lacunar infarction (LI) and intracerebral hemorrhage (ICH). It is generally postulated that, in its early stages, the vascular lesion engenders vessel wall fragility and ICH. But if rupture dose not occur, segmental vessel occlusion evolves, producing LI. It may be a common aging phenomenon that is exacerbated by hypertension and diabetes mellitus. This study was performed to evaluate risk factors of LI and ICH. We reviewed retrospectively the medical records of 690 patients (254 LI, 436 ICH) with stroke who were admitted to Keimyung University Dongsan Medical Center from January 1995 to December 1998. The major risk factors including age, sex, hypertension, diabetes mellitus and several minor risk factors were analyzed in these patients. Older age (p<0.01), male sex(p<0.01), hypertension with treatment(p<0.01), hypertension with long duration(p<0.01) and hypertriglyceridemia(p<0.01), cigarette smoking(p<0.01) were significantly more frequent in LI. Younger age(p<0.01), female sex(p<0.01) and hypertension without treatment(p<0.01), hypertension with short duration(p<0.01) were more frequent in ICH. The prevalence, duration and treatment of diabetes mellitus were not different between these two groups. This study show that the age, sex, treatment of hypertension, duration of hypertension and cigarette smoking seem to be significant risk factors between LI and ICH.