돼지감자 분말을 이용한 고정화 Kluyveromyces marxianus sp.의 에탄올 연속발효

신지현,최언호 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.3

돼지감자 분말로부터 에탄올을 효율적으로 생산하기 위하여 Kluyveromyces marxianus F043을 2% sodium alginate에 고정화시키고 연속발효를 수행하여 발효특성을 조사하였다. 연속발효 수행시 고정화 효모는 48시간으로 활성화되었다. 2% cellulase로 처리된 돼지감자배지의 CSTR 연속발효에서 희석속도가 증가할수록 에탄올 농도는 감소하였고 잔당 농도는 증가하였다. 에탄올 생산성이 높고 당의 유실율이 낮은 최적조건은 희석속도 0.2h^(-1), 유입 배지농도 75 g/ℓ이었으며 이때 에탄올 생산성은 3.1 g/ℓ·h, 기질이용율은 62.6%이었다. 최적조건에서 3주 동안 연속발효를 수행하였을 때 유출액 중의 에탄올 농도는 16.3∼17.9 g/ℓ, 생균수는 6.60∼7.16 log cells/㎖로 거의 일정하게 유지되었고 에탄올 이외에 methyl, n-propyl, iso-butyl, iso-amyl alcohol이 미량 검출되었다. To produce ethanol from Jerusalem artichoke powder efficiently, Kluyveromyces marxianus F043 cells were encapsulated in 2% sodium alginate and were cultured in a countinuous reactor to investigate the fermentation properties. Immobilized K. marxianus F043 cells were activated for 48 hours in a fermentor for continuous ethanol production. The culture in a CSTR using a Jerusalem artichoke substrate treated with 2% cellulase showed a decrease in ethanol concentration and an increase in residual saccharide concentration with a increasing dilution rate. Optimum conditions for high ethanol productivity and low residual saccharide outpu were clarified to be given at a dilution rate of 0.2 h^(-1) and a Jerusalem artichoke medium concentration of 75 g/ℓ. Ethanol productivity of 3.1 g/ℓ·h and saccharide utilization of 62.6% were obtained under the optimum condition. When the fermentation was performed for 3 weeks under these conditions, the effluent medium showed stable ethanol concentrations of 16.3∼17.9 g/ℓ and viable cells of 6.60∼7.16 log cells/㎖ without contamination. Trace amounts of methyl, n-propyl, iso-butyl, iso-amyl alcohols besides ethanol were detected.

섬애약쑥 주정 추출물로 제조한 발효식초의 품질특성

신지현,강민정,변희욱,배원열,신정연,서원택,최진상,신정혜,Shin, Ji Hyeon,Kang, Min Jung,Byun, Hee Uk,Bea, Won Yoel,Shin, Jeong Yeon,Seo, Weon Tack,Choi, Jine Shang,Shin, Jung Hye 한국식품저장유통학회 2017 Food Science and Preservation Vol.24 No.5

This study was carried out to investigate the quality characteristics of vinegars containing jaceosidin and eupatilin using Artemisia argyi H. ethanol extract (AEE). 10% malt extract (ME) and water extract of Artemisia argyi H. (AWE) were also prepared for vinegar production. Three kinds of materials were mixed in the same amount to prepare vinegar as follows; CO (ME, water, 18% edible ethanol), SE (ME, water, and AEE), SW (ME, AWE, and 18% edible ethanol) and SM (ME, AWE, AEE). All samples were fermented by Acetobacter pasteurianus A8 at $30^{\circ}C$ for 25 days and analyzed at 10, 15, 20 and 25 days. The pH decreased significantly during the fermentation. pH was lower in SE and SM than CO and SW. The acidity increased significantly during the fermentation, and was highest in SM (4.44%) at 25 days of fermentation. The concentration of acetic acid was higher than other organic acids for all vinegars. Jaceosidin and eupatilin were not detected in both CO and SW, but both were detected in the SE and SM. At 25 days of fermentation, jaceosidin and eupatilin concentrations in SE and SM were 6.49-6.88 mg/kg and 2.23-2.24 mg/kg, respectively. From these results, we confirmed that production of vinegar containing jaceosidin, eupatilin and phenolic compounds can be prepared by using Artemisia argyi H. edible ethanol extract.

운영현황 분석을 통한 지열 히트펌프 시스템의 경제성평가

신지현,이진현,조영흠,김용인 한국건축친환경설비학회 2018 한국건축친환경설비학회 논문집 Vol.12 No.5

In this study, economic analysis is carried out by applying the geothermal input/output temperature, power consumption and performance information based on the actual operating data of the geothermal heat pump system. The economic is analyzed and compared with the chiller-heater system and geothermal heat pump system which applied the existing economic analysis method. As a result, the initial investment cost of geothermal heat pump system was higher than that of the chiller-heater system but the operation cost was reduced. It is also confirmed that the payback period is shortened when the economic is analyzed according to the operating data as compared with the existing economic analysis method of the geothermal heat pump system. In this study, the economic analysis result changes according to application of operating data and application of operating data is necessary for accurate economic analysis.

지열시스템 순환펌프 변유량 제어를 활용한 운영 방안개발

신지현,최동희,조영흠 한국건축친환경설비학회 2018 한국건축친환경설비학회 논문집 Vol.12 No.5

The purpose of this study is to develop an operating method of geothermal heat pump system to prevent waste of energy due to inefficient operation. As the interest in energy saving is increasing, the development and use of renewable energy systems is increasing. In korea, While the geothermal system with excellent performance is installed, the other heat source system is used as the main heat source, or the system operates in the partial load state, resulting in waster of energy by controlling the fixed flow rate of the geothermal circulation water. Therefore, the purpose of this study is to develop an operating method of several geothermal heat pump system through the variable water flow rate control method of circulation pump. The proposed operating method is tested through simulation

Autophagy Regulates Formation of Primary Cilia in Mefl oquine-Treated Cells

신지현,배동준,김은성,김한별,박소정,주윤경,조두신,조동규,김상엽,조동형 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.4

Primary cilia have critical roles in coordinating multiple cellular signaling pathways. Dysregulation of primary cilia is implicated in various ciliopathies. To identify specifi c regulators of autophagy, we screened chemical libraries and identifi ed mefl oquine, an anti-malaria medicine, as a potent regulator of primary cilia in human retinal pigmented epithelial (RPE) cells. Not only ciliated cells but also primary cilium length was increased in mefl oquine-treated RPE cells. Treatment with mefl oquine strongly induced the elongation of primary cilia by blocking disassembly of primary cilium. In addition, we found that autophagy was increased in mefl oquine-treated cells by enhancing autophagic fl ux. Both chemical and genetic inhibition of autophagy suppressed ciliogenesis in mefl oquine-treated RPE cells. Taken together, these results suggest that autophagy induced by mefl oquine positively regulates the elongation of primary cilia in RPE cells.

부모의 학력에 따른 아동의 안전실천행동

신지현 한국보육학회 2018 한국보육학회 학술대회자료집 Vol.2018 No.-

공간 콜래보레이션이 적용된 상업공간사례 검토 - 헤이리 마을의 4사례 검토를 통하여 -

신지현,신경주 한국실내디자인학회 2015 한국실내디자인학회논문집 Vol.24 No.3

Social and cultural impact of postmodernism in the seek for diversity and to respect the individual's personality and character was the center of human-centered thinking. This also affects the design area, as well as architecture, interior design. It is because of interactive ​​interior design that reflects the lives and relationships with people. Thus, in modern society, people to meet the diverse needs have created a third space. Today's commercial space in the flow to a variety of users and is trying to provide comfort. To go to stores often offer lifestyle and proceeding to the next stage of life. Space for the creative force is trying to become getting better space. Collaboration is refer to phenomenon that two or more objects meet to collaborate. Collaboration is can be classified of space, art, low/high, high concept and star collaboration. And this study has been studied mainly collaboration. Space collaboration is can be classified of experiential, the third space, brand passes and landmark. Space collaboration elements rearranged to the standards of interior design theory, analysis, and marketing of space, commercial space, with previous studies of the spatial elements were extracted. The purpose of this study in the field of interior design space analysis method for the verification of collaboration theory is that to develop. Thus, the space collaboration has been developed to fit the field of interior design, and commercial interior spaces will be helpful for applying. 인간 중심적인 사고의 포스트모더니즘의 다양성에 대한 추구와 문화적 사회적 영향으로 개인의 인격과 품성은 존중해야 하는 대상이었다. 이것은 또한 디자인 뿐 아니라 건축과 인테리어 디자인에도 영향을 미친다. 그것은 사람들의 삶과의 관계를 함께 반영하는데 이는 인테리어가 상호 작용의 관계이기 때문이다. 따라서 현대 사회에서 사람들의 다양한 요구를 충족시키기 위해서 제3의 공간을 만들었다. 오늘의 상업 공간은 사용자의 다양한 요구에 대해서 안락한 공간을 제공하려고 노력하고 있다. 상점에 가는 것이 가끔 생활 방식과 인생의 다음 단계로의 방향을 제공하기도 한다. 그것이 창의적인 힘을 위한 공간을 만들고 자 더 나은 세상을 향해서 노력하고 있다. 콜래보레이션이란 둘 이상의 개체 상호 간에 협업하는 현상을 언급한다. 콜래보레이션은 공간, 예술, 저급개념/ 고급개념, 고차적인 개념과 스타적인 협업으로 분류될 수 있다. 이 연구는 주로 협업에 대해서 연구되어 왔다. 공간 콜레보레이션은 경험적인 것과 제3의 세계, 브랜드 패스와 랜드마크로 분류될 수 있다. 공간 콜레보레이션 요소는 다시 표준적인 인테리어 디자인 이론, 분석, 그리고 공간 마케팅, 상업 공간의 다양한 기준 등에 대해서 검토 되었다. 이 연구는 이전 연구들에서 공간 요소들을 추출하고 이를 통하여 재조정이 이루어졌다. 본 연구에서는 갤러리, 아트샵, 카페로 콜래보레이션 된 상업공간디자인의 각 공간별 검토를 위하여 이때 필요한 요소를 탐색하며, 탐색된 요소로 각 공간별 콜래보레이션 효과 검토를 시도하였다. 그러므로 공간 콜래보레이션에 맞추어서 상업 공간의 내부공간을 적용하고 자 할 때에 이 연구 결과가 많은 도움이 될 것으로 기대한다.

Bacillus subtilis와 Listeria monocytogenes의 일반 스트레스반응의 비교

신지현,Shin, Ji-Hyun 한국미생물학회 2009 미생물학회지 Vol.45 No.1

A diverse range of stresses such as heat, cold, salt, ethanol, oxygen starvation or nutrient starvation induces same stress-responsive proteins. This general stress response enhances bacterial survival significantly. In Bacillus subtilis and closely related Gram-positive bacteria Listeria monocytogenes, the general stress response is controlled by the alternative transcription factor ${\sigma}^B$. The activity of ${\sigma}^B$ is regulated post-translationally by a signal transduction network that has been extensively studied in B. subtilis, and serve as a model for L. monocytogenes. The proposed model of L. monocytogenes signal transduction network is similar to that of B. subtilis, but the energy stress pathway is missing. More than 150 general stress proteins belong to ${\sigma}^B$ regulon of B. subtilis and L. monocytogenes. In both bacteria, ${\sigma}^B$ function is primarily important for resistance to diverse stresses. In addition, ${\sigma}^B$ function contributes to the control of important virulence genes in food-borne pathogen L. monocytogenes. Therefore, understanding of the general stress response is important not only for bacterial physiology, but also for pathogenicity.

초심을 잃지 않는 연구자를 꿈꾸며

신지현 한국생물공학회 2012 BT NEWS Vol.19 No.2

디지털 프린터의 출려특성기반 톤 보정 망점화

신지현,박희윤,신동률,안석출 한국인쇄학회 1997 한국인쇄학회지 Vol.15 No.1