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Bee Venom 및 Melittin 약침액(藥鍼液)이 신경아세포종(神經芽細胞腫)의 세포사(細胞死)에 미치는 영향(影響)
강동철,정태영,서정철,임성철,한상원,Kang, Dong-cheol,Jung, Tae-young,Seo, Jung-chul,Leem, Seong-cheol,Han, Sang-won 대한침구의학회 2003 대한침구의학회지 Vol.20 No.2
Objective : This study was designed to analyze the effects of bee venom and melittin on cell death in neuroblastoma cell line. Methods : MTT assay, morphologic method, DNA fragmentation, flow cytometry, immunocytochemistry analysis, RT-PCR and Western blot were performed. Results : The obtained results are summarized as follows: 1. The MTT assay demonstrated that neuroblastoma cell viability was significantly inhibitted dose-dependently by treatment with bee venom and melittin in comparison with control. 2. Cell culture demonstrated that control group proliferated highestly at he 5th day in comparison with the 4th day in bee venom and melittin group. And in bee venom and melitti group cell proliferation decreased 2.5 times than control group. 3. The morphologic study demonstrated that neuroblastoma cell showed apoptosis after treatment with bee venom and melittin for 6 hours using microscope. 4. The Flow cytometry demonstrated that apoptosis of neuroblastoma cell treated with bee venom and melittin was related with stop of cell cycle in stage of $G_0/G_1$. 5 .DNA fragmenation demonstrated that neuroblastoma cell treated with bee venom and melittin showed DNA ladder below 1 Kbp. 6. Immunocytochemistry assay demonstrated that Fos and MAPK which are related with cancer were down-regulated by treatment with bee venom and melittin. 7. RT-PCR analysis demonstrated that Fos and MAPK mRNA were transcripted. Fos was down-regulated form treatment with $5{\mu}g/ml$ bee venom and MAPK was down-regulated form $1{\mu}g/ml$ bee venom. 8. Western blot demonstrated that Fos was down-regulated from $1{\mu}g/ml$ bee venom whereas MAPK was expressed by $1{\mu}g/ml$ bee venom but down-regulated by $10{\mu}g/ml$ bee venom. Conclusions : We found that some cancer related genes ware down-regulated by treatment with bee venom and melittin. Further study is needed for investigating the anti-cancer effect of bee venom and melittin.
순간흡수 분광학 측정장치 구성 및 DDI의 순간흡수율 변화 측정
서정철,이민영,김동호,정홍식,박승한,김웅,Seo, Jung-Chul,Lee, Min-Yung,Kim, Dong-Ho,Jeong, Hong-Sik,Park, Seung-Han,Kim, Ung 한국광학회 1991 한국광학회지 Vol.2 No.4
최근들어 극초단 광학펄스를 생성하고 증폭하는 기술의 발달로 인하여 시분해 레이저 분광학에 많은 영향을 끼치게 되었다. 그 중에서도 응용성이 넓은 순간흡수분광학장치를 본 연구실에서 개발하였다. 이 기술은 고출력 레이저 펄스를 이용하여 white light continuum pulse를 생성시켜 이를 조사빔으로 이용하고, pump pulse는 원래 상태의 레이저를 이용하는 것이다. 순간흡수율 변화를 여러 파장에서 측정가능하도록 photodiode array를 이용하였으며, pump와 probe pulse의 시간차이에 의한 순간흡수율 변화를 색소의 일종인 DDI를 이용하여 측정하였다. Recently, the developments in generating and amplifying ultrashort optical pulses $(ps=10^{-12}s or fs=10^{-15}s)$ have imposed on great advances in the time-resolved laser spectroscopy. Especially, the transient absorption spectroscopy has a wide application range and the main idea of this technique is pump & probe method. After the pump pulse makes the material an excited or a transient states, the probe pulse is sent through the material to measure the absorbance change due to the transient states. Here, if the absorbance change was measured by the time delay between pump & probe pulses, the dynamic information of the excited or the transient states (the transient abnsorption changes by time & wavelength) can be obtained. At our laboratory, the ultrashort optic1 pulse (<ps) was generated and amplified to fabricate the transient absorption measurement system and the transient absorption change of DDI dye was measured in ethanol.
일제치하(日帝治下)부터 1970년대까지의 한국(韓國) 침구학사(鍼灸學史)에 대한 고찰
서정철,남상수,안병철,Seo, Jung-Chul,Nam, Sang-Soo,Ahn, Byung-Cheol 대한침구의학회 2001 대한침구의학회지 Vol.18 No.4
Objective ; This Study was carried out to review the history of acupuncture & moxibustion in Korea under the control of imperialized Japan and to compare the history among Korea, China and Japan. Results ; Under the control of imperialized Japan the science of acupuncture & moxibustion in Korea has experienced the dark ages because of the momentary disordered medical laws made by Japan. As we compare the history of acupuncture & moxibustion among Korea, China and Japan, Korea was simple but systemic, China was complicate but complex and Japan was nearly surgical. Conclusion ; The science of acupuncture & moxibustion of Korea was mostly advanced in the late Chosun and did not just imitate that of China and has advanced uniquely.
서정철,임강현,한상원,Seo, Jung-Chul,Leem, Kang-Hyun,Han, Sang-Won 대한약침학회 2003 Journal of pharmacopuncture Vol.6 No.1
Bee venom has been clinically used to control the pain of inflammation disease etc. in general. Although the effect of bee venom herbal acupuncture has been reported, its mechanism has not been fully elucidated yet. Free radical metabolism seems to occupy a remarkably common position in the mechanisms of inflammation and ageing related disease. Oxidative damage to DNA, lipids, proteins and other molecules may contribute to the development inflammation disease. NO or DPPH is one of the free radicals and a mediator in inflammation diseases. The objective of this study is to investigate the scavenging effect of bee venom herbal acupuncture against NO and DPPH. The followings are the summary of the results: (1) There is no significant scavenging effect of bee venom herbal acupuncture on NO in BVS and BVP group. (2) There is a significant scavenging effect of bee venom herbal acupuncture on DPPH in BVS-1 and BVP-1 group. These results suggest that bee venom herbal acupuncture can be used for inflammation diseases such as rheumatoid arthritis. This study would provide important basic data on the possibility of the clinical treatment of bee venom herbal acupuncture. Further studies are required to investigate the antioxidative effects of it.
CC, CF, BV 약침의 침감에 대한 임상적 연구 -placebo 약침을 위한 기초연구-
서정철,윤종석,한상원,박히준,이혜정,Seo, Jung-Chul,Yoon, Jong-Seok,Han, Sang-Won,Park, Hi-Joon,Lee, Hye-Jung 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.1
Objective : This study was designed to find out whether NS(normal saline) is able to be constituted as an appropriate control group for CC(Cervi Cornu Parvum herbal acupuncture), CF(Carthami-Flos herbal acupuncture) and BV(bee venom herbal acupuncture). Method : NS and three herbal acupuncture were inserted into Quchi(LI 11) of the subjects. After 5 minutes the subjects completed a questionnaire rating the intensity of 21 kinds of acupuncture sensation; hurting, penetrating, sharp, aching, intense, spreading, radiating, tingling, pricking, stinging, pulling, heavy, dull, numb, electric, shocking, hot, burning, cool, pulsing, and throbbing. We compared subjective evaluations of acupuncture sensation between or among the groups. Results : As for CC half items of the acupuncture sensation were significantly different from NS. As for CF all items were not significantly different from NS. As for CC all items were significantly different from NS except one item. In general the score of CF acupuncture sensation was lower than the others and the score of BV acupuncture sensation was higher than the others from comparison of sensation among herbal acupunctures(CC, CF, BV). Conclusion : We found that NS is able to be an appropriate placebo herbal acupuncture for CF. Further study is needed for new placebo herbal acupuncture for CC and BV.
서정철,이재동,박동석,강성길,안병철,김이화,김순애,이희제,김창주,정주호,Seo, Jung-Chul,Lee, Jae-Dong,Park, Dong Suk,Kang, Sung-Keel,Ahn, Byung-Cheol,Kim, Ee-Hwa,Kim, Soon Ae,Lee, Hee Jae,Kim, Chang-Ju,Chung, Joo-Ho 대한침구의학회 2001 대한침구의학회지 Vol.18 No.3
목적 : 본 연구는 최근 임상에서 많이 사용하는 자하차(紫河車) 약침액(藥鍼液)이 과산화수소($H_2O_2$)로 야기된 송과선 세포의 apoptosis에 있어서 세포 보호에 미치는 영향과 그 기전을 분석하였다. 방법 및 결과 : MTT assay를 이용하여 분석한 결과 처리 시간 및 농도에 따른 세포 독성의 효과가 $H_2O_2$ 투여로부터 관찰되었다. 또한 자하거 약침액은 세포 증식 효과를 나타내었고 자하거 약침액을 전처치하고 $H_2O_2$를 처치하였을 때 세포 독성이 크게 감소되었다. DAPI staining에서 자하거 약침액 투여군은 $H_2O_2$ 투여군에 비해 fragmentation이 억제되었다. TUNEL assay를 통하여 자하거 약침액 투여군은 $H_2O_2$ 투여군에 비하여 TUNEL 양성세포의 발현이 감소되었다. Flow cytometry를 통하여 자하거 약침액 투여군은 $H_2O_2$ 투여군에 비하여 세포주기 중 sub G1 분획의 증가가 억제되었다. 결론 : 이상의 결과를 통하여 자하거 약침액이 $H_2O_2$로 유발된 apoptosis에서 세포보호 효과가 있음이 확인되었다.