Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

腦卒中患者에 倂發된 肺炎에 대한 東西醫學 協力治療의 臨床的 硏究 : 抗生劑 使用實態와 死亡率을 中心으로

尹相協,柳在煥,景銀培,羅嶺雪,閔炳一,金裕國,金鍾烈,柳基遠 경희대학교 1992 慶熙醫學 Vol.8 No.4

Prednisolone과 Prednisone의 임상약동학적 평가

신재국,윤영란,차인준,이경훈,장인진,신상구,박찬웅 大韓臨床藥理學會 1994 臨床藥理學會誌 Vol.2 No.1

분리 대두단백의 소화율과 전기영동패턴에 미치는 Phytate의 영향

윤재영,조희경,이서래 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-

Phytate 함량이 다른 두 분리 대두단백(HSPI; high-phytate soy protein isolate, LSPI; low-phytate soy protein isolate)을 제조하여 pH 및 phytate 첨가량이 그들의 용해도와 소화율에 미치는 영향을 알아보았고 분리 대두단백을 용해도에 따라 분별한 후 그들의 특성을 전기영동을 통하여 알아보았다. LSPI와 HSPI 모두 분자량이 13.6~81.1 kDa 에 이르는 단백질 성분으로 이루어져 있으며 산성 pH에서 18.0~35.0 kDa의 단백질은 phytate와 잘 결합하지 않는 특성을 가졌다. LSPI를 용해도에 따라 분별하였을 때 gliadin을 구성하는 단백질은 pH2에서 phytate와 쉽게 결합하지 않았다. 대두 단백질은 pepsin 소화율은 phytate 첨가량이 증가할수록 크게 저하되었다. This study was carried out to examine the effects of phytate addition on the solubility and digestibility of the low-phytate soy protein isolate (LSPI) and high-phytate soy protein isolate (HSPI). In SDS-polyacrylamide gel electrophoresis of soy protein isolate, different patterns of proteins were observed in both HSPI and LSPI at various phytate and pH levels, suggesting that phytate may bind specifically to certain protein fractions at a particular pH. For example, proteins of M.W. 1.8~3.5kDa resisted phytate binding at acidic pH. LSPI was fractionated into albumin, globulin, gliadin and glutelin; and phytate was shown to bind with difficultly to all three gliadin bands. Effects of phytate on the pepsin digestibility of soy proteins were apparent, especially in the short term digestion.

사무직 근로자들의 카드뮴에 대한 노출정도

이경재,김주자,황정호,허지회,김강윤 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

..

드레인 전류 잡음원만을 고려한 스케일링이 가능한 바이어스 의존 P-HEMT 잡음모델

윤경식,이창석,맹성재,이진희,박철순 대전산업대학교 반도체기술연구소 1999 半導體技術硏究所報 Vol.1 No.-

게이트 길이가 0.2㎛인 P-HEMT에 대하여 드레인 바이어스 전류의 변화 및 게이트 폭에 대해 스케일링이 가능한 잡음모델을 제안하였다. 본 논문에서는 S-파라미터를 정확히 예측하기 위하여 x를 제외한 intrinsic 파라미터는 offset를 도입하여 정규화 한 후 스케일링을 하였다. 드레인 포화전류에 대한 드레인 전류의 비율과 게이트 폭을 변수로 하는 소신호 모델 파라미터의 맞춤함수를 구하였다. 또한, 잡음 파라미터를 정확히 예측하기 위하여 진성저항 잡음 온도 T_g, 게이트 단 전류 잡음원 등가잡음 컨덕턴스 G_ni 드레인 단 전류 잡음원 등가잡음 컨덕턴스 G_no를 잡음 모델정수로 사용하여 그 값을 추출하여 스케일링 하였다. 잡음 온도 T_g는 드레인 전류와 게이트 폭에 거의 추출되었다. 그러므로, G_no만을 잡음 모델정수로 하는 잡음모델과 T_g, G_ni, G_no를 잡음 모델정수로 하는 잡음모델을 측정값과 비교하여 본 결과 G_no만을 갖는 잡음모델도 측정된 잡음 파라미터와 잘 일치하였다. 따라서, 모델 정수 추출이 간단한 G_no 만을 갖는 잡음모델은 게이트 폭과 바이어스 전류에 대해 스케일링이 가능한 실용적인 잡음모델임을 확인하였다. Bias-dependent noise models of 0.2㎛ gate length P-HEMT's which are scalable with gate width are proposed. To predict S-parameters of the P-HEMT's the intrinsic parameters except for x subtracted the offsets introduced in this paper are normalized to the gate width and then scaled. The small-signal model parameters are expressed as fitting functions of the drain current to I_dss ratio and gate width. In addition, to estimate accurately noise parameters the noise temperature T_g of the intrinsic resistance, the equivalent noise conductance G_ni of the gate current noise source, and the equivalent noise conductance G_no of the drain current noise source are adopted as the noise model parameters. The extracted values of T_g are nearly independent of drain current and gate width and their average is arou

오존처리·추출처리후 섬유벽내의 탈리그닌

尹承洛,金在慶 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

추출조작중 알칼리용액 추출이 물에 의한 추출보다 탈리그닌, 강도적 성질이 우수하다는 원인을 구명하기 위하여 자외선 현미경을 이용하여 섬유벽내의 리그닌 분포 및 탈리그닌 정도를 관찰한 결과는 다음과 같다. 1. 물과 1% NaOH 용액에 의한 추출처리에서 용액에 따라 탈리그닌 영역이 다르다. 2. 오존에 의해 산화 분해된 섬유내 리그닌의 용출은 물보다 1% NaOH 용액이 효과적이었다. 이런 이유로 물에 의한 추출보다 1% NaOH 용액으로 추출한 펄프의 강도적 특성이 우수하다. 3. 고온(70℃)에서 추출처리를 행하면 섬유표면과 루멘측의 리그닌이 동시에 용출된다. 섬유로 부터의 리그닌 용출은 실온보다 고온에서 처리하는 것이 효과적이다. In order to evaluate the delignification in cell wall by alkali extraction treatment after ozonation, the lignin distribution and delignification degree was determined by UV microscope. The delignification area was differentiated depending on extractants such as water or 1% NaOH. The content of oxidized gushing out lignin in fiber was higher by 1% NaOH extraction than by water, therefore, the strength properties of pulp extracted with 1% NaOH was better. When the extraction treatment was carried out at high temperature(70℃), the lignin was simultaneously gushed out in the surface of fiber and lumen, therefore the treatment at high temperature was preferred for the purpose of lignin gushing out from fiber.

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

( Jae-sung Ryu ),( Kyu-tae Chang ),( Ju-taek Lee ),( Malg-um Lim ),( Hyun-ki Min ),( Yoon-ju Na ),( Su-bin Lee ),( Gislain Moussavou ),( Sun-uk Kim ),( Ji-su Kim ),( Kinarm Ko ),( Kisung Ko ),( Kyung- 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.6

The ACKNOWLEDGEMENTS should be corrected as follows, "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea. The published article in this journal was a part of the 2013 Doctoral thesis of Jae-Sung Ryu submitted to Wonkwang University, Korea." and not "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea."

가루깍지벌레의 生態와 防際에 關한 硏究

尹柱敬,李載窪 全南大學校 農漁村開發硏究所 1974 農業科學技術硏究 Vol.8 No.-

This payer contains some results investigation into bionomics and insecticides of Pseudococcus comstocki daring April to December 1973. The summary of the findings are as follows: 1. Occurrence was most abundant on May 30 for its first generation; on July 20 for the second generation; and on November 10 for the third generation. It passed the winter season in the form of nymph adult or egg. The eggs were usually laid in the underground of 3cm to 100cm below the surface numbering an average of 140 eggs. The percentage hatched was 93.4%. 2. Fruits most seriously damaged by the insects were in order of pear, apple, and persimmon. Among pear fruit the most sensitive variety were in order of Suckli, Sinkoo, Mansamkil and Jangsimlang. 3. The number of nymph at the first generation began to increase after 10 days from inoculation. The number of the second generation nymph reached its maximum on July 20 and began to decrease as the time passed. 4. The adult insects raised in the laboratory room facing south laid an average of 135.1 eggs. On the other hand, those raised in the room facing north had 102.9 eggs; on the average. The inserts raised in the south-facing room had finished the laying; of eggs 4-5 days earlier than the insect raised in the north-facing room. 5. The winter-passed-larvae moved their location during May and July. The larvae dwelling in the north parts moved faster than those in the root parts, but later than those in the south parts. 6. The experiment of larvae-hatching at varying temperature showed that it took 30-45 days at 15℃; 14 days at 20℃; and 10 days at 25℃ during April. At 5℃ the larvae were not hatched at all. In May the length of hatching time was a little shorter than that in April. 7. As shown in Table 12 and 13, the most effective chemicals in controlling the insects were in order of Folidol, Loxoun and Sevin. 8. Owing to the ant's disturbing activities against the parastic wasps of mealbugs, the insects were relatively safe and the damage cased by them were more serious. 9. The natural enemy collected were: Anaggrus suballipes Ishii, Allotropa burrelli Muesbeck, Scymnus phosphorus Lewis.