채식자와 비채식자에서 혈청 지질, 지단백 및 총 항산화능의 비교

김수길(Su Gil Kim),김현일(Hyun Il Kim),엄민섭(Min Sup Yum),조호(Ho Jo),오용열(Yong Yeol O),권한진(Han Jin Kwan),조욱현(Wook Hyun Cho),박정식(Jeong Sik Park) 대한내과학회 2000 대한내과학회지 Vol.58 No.2

N/A Background : Previous studies have demonstrated that modified or oxidized lipoproteins play a key role in the process of atherogenesis, particularly in hyperlipidemic individuals. Low density lipoprotein(LDL) is modified by oxygen free radical from damaged tissue or inflammatory cells. Further changes in the LDL molecule lead to an oxidized form (oxidized LDL), which is recognized by the macrophage scavenger receptor. Scavenger receptors on macrophages recognize and bind oxidized LDL. As uptake continues, the macrophages change to lipid-laden foam cells, the components of the fatty streak, which is the precursor atherosclerotic lesion. Antioxidants are known to prevent modification of LDL by free radicals and possibly also atheroma formation. This study was designed to compare the total antioxidant status and other lipid profiles in vegetarian and non-vegetarian groups in Korean adults to see the effect of diet modification on antioxidant status. Methods : 174 vegetarian and 150 non-vegetarian male adults were recruited for lipid test including total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, lipoprotein(a), and total antioxidant status during annual routine physical examination. Result : 1) Total antioxidant status was significantly high in vegetarian group(1.390±0.288 mmol/L) compared to non-vegetarian group(1.155±0.290 mmol/L)(p<0.001). 2) In non-vegetarian group, total antioxidant status was significantly low in smokers(1.041±0.288 mmol/L) compared to non-smokers(1.227±0.328 mmol/L)(p<0.001). 3) Correaltions between lipid profiles and total antioxidant status in vegetarian and combined groups were not significant. Conclusion : Total antioxidant status in vegetarian group was significantly higher compared to non-vegetarian group. Among non-vegetarian group, smoker group showed lower total antioxidant status compared to non-smoker group. And there was no significant correlations between lipid profiles and total antioxidant status.(Korean J Med 58:197-203, 2000)

만성 심혈관계 환자에서 식이와 생활양식에 따른 총항산화능의 변화

장윤정,송경은,박의현,최영선,이난희 대한임상병리학회 1999 대한임상병리학회지 Vol.19 No.5

만성 심혈관계 환자에서 식이와 생활양식에 따른 총항산화능의 변화

장윤정,송경은,박의현,최영선,이난희 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

배경:체내 항산화계의 기능이 저하되거나.자유라디칼이 과잉생성되면 심혈관계질환이나 암을 비롯하여 여러 질병을 유발할 수 있다.항산화계에 영향을 미치는 인자로는 세포내 항산화 효소나, 알부민, 요산 등의 항산화물질 뿐 아니라 항산화 비타민을 포함한 식이,운동 등 생활양식의 차이도 중요하며, 최근 TAS kit가 개발되어 혈중 총항산화능을 손쉽게 측정할 수 있게 되었다.이에 저자들은 만성심혈관계 질환 환자들을 대상으로 생활양식에 따른 총항산화능의 변화를 살펴 보았다. 방법:만성심혈관질환자 66명과 건강한 대조군 63명을 대상으로 식습관 및 생활양식 등을 설문조사하고, 다음날 아침 공복시에 채혈하였으며, 환자군중 34명은 3개월 후 다시 조사하였다.채취한 혈액으로 총항산화능은 Hitachi 747을 이용하여 TAS kit로, 비타민 A와 E는 HPLC방법으로,그리고 Hitachi 7060을 이용하여 혈청 지질치를 각각 측정하였다. 결과: 환자군과 대조군에서의 총항산화능 값은 각각 1.18mmol/L. 1.19mmol/L로 차이가 없었고, 3개월 후 환자군의 총항산화능 값에서는 유의한 증가를 나타내었다.HDL- 콜레스테롤 값은 영양교육 후 유의한 상승을, 총 콜레스테롤과 중성지방,Lp(a)은 의미있는 감소를 보였고, 비타민 A와 비타민 E 값도 경미한 상승을 보였다.생활양식 중에서는 음주 상태에 따른 총항산화능 값에서 통계적으로 의미있는 차이를 나타내었다. 결론: 심혈관계 질환에서 총항산화능 측정이 산화스트레와 항산화 상태를 반영하는 수단으로 이용될 수 있으며, 음주, 항산화비타민의 섭취 등이 총항산화능에 영향을 미칠 것으로 사료된다. Background: Decreased antioxidant defense mechanism and over-generated free radicals by either narmal or aberrant metabolism play an important role in the pathogenesis of cardiovascular disease and cancer.And the life style and diet including vitamin A and E have been thought as important factor in antioxidant systems.Recently, Total Antioxidant Status kit(TAS, Randox, UK) has been developed for measuring the total antioxidant capacity of blood.So we evaluated the association of life style and total antioxidant capacity in patients with chronic cardiovascular disease. Methods: Sixty six cases of chronic cardiovascular disease and sixty three normal controls were evaluated Serum total antioxidant capacity was measured by Hitachi 747(Hitachi Ltd, Japan) with TAS kit, vitamin A and E were measured by high performance liquid chromatography method, and serum lipid profile by Hitachi 7060(Hitachi Ltd, Japan). Results: the mean total antioxidant capacity did not show difference between patients and controis.After 3 momths, we detected significant increase fo total antioxidant capacity, high-density lipoprotein cholesterol and mild increase of antioxidant vitamins and significant decrease of total cholesterol, triglyceride, lipoprotein(a).The heavy alchol consumption group showed significant difference with other groups(p<0.05). Conclusions: The measurement of total antioxidant capacity for the cardiovascular disease patients was thought to be useful to evaluate oxidative stress and antioxidant status.Alchol consumption and supplementation with antioxidant vitamins could have an effect on total antioxidant capacity.(Krean J clin Pathol 1999;19:504-9)

울산지역 노인의 생활습관에 따른 항산화 영양소 섭취실태 및 혈액내 항산화 영양 상태 비교

김미정,김옥현,김정희 대한지역사회영양학회 2002 대한지역사회영양학회지 Vol.7 No.4

Body antioxidant status is an important factor in the prevention of many chronic diseases caused by oxidative stress, especially in the elderly and is affected by health-related habits, such as smoking, drinking and regular physical activity. The aim of this study was to investigate the relationship between these health-related habits and plasma antioxidant status in the elderly. Plasma antioxidant status was examined by determining plasma levels of antioxidant vitamins (vitamin C, A, E, $\beta$ -carotene), total antioxidant status (TAS) and thiobarbituric acid-reactive substance (TBARS) . The subjects included 225 elderly persons aged over 60 yews (63 males, 162 females) living in the Ulsan area. They were interviewed to collect data on their general characteristics and health behaviors such as smoking, exercise and alcohol consumption by means of questionnaires. Their dietary intakes were obtained by means of semi-quantitative food frequency questionnaires (FFQ) The study population was divided into two or three groups according to their smoking, drinking, and exercise status. The ratio of smoker, drinker and exerciser was 16.7%, 31.0% and 44.2% respectively. The dietary antioxidant vitamin intakes were not significantly different among groups of smoking and drinking status, but tended to be higher in non-smokers and non-drinkers than in smokers and drinkers. Plasma vitamin C and $\beta$ -carotene levels were significantly higher in non-smokers, but Plasma vitamin A and TBARS levels were significantly lower in non-smokers than in smokers. Plasma TAS was not significantly different among the smoking groups, but showed a tendency to decrease with an increase in the number of packyear. Plasma vitamin C and $\beta$ -carotene levels of the non-drinkers were higher than those of drinkers and past-drinkers, but plasma vitamin A, C, E, TAS and TBARS showed no difference among the groups of drinker. All vitamin intakes of the exercisers were slightly higher than those of the non-exercisers, but vitamin C intake was significantly higher in female exercisers than in non-exercisers. Plasma $\beta$ -carotene levels were significantly higher in male exercisers and plasma vitamin A, C, E, TAS and TBARS levels tended to be higher in exercisers than in non-exercisers. These results suggested that change to non-smoker, modulation of alcohol consumption and regular exercise could enhance antioxidant defences against reactive oxygen species and might increase the likelihood of a healthier life span.

운동영양학 : 인삼복용시 과산화지질과 총항산화능에 미치는 영향

김승환(SeungHwanKim),장명재(MyoungJeiChang),이성규(SungGyuLee),장완성(WanSungChang),최현희(HyunHeeChoi),성종환(JongHwanSung) 한국체육학회 2003 한국체육학회지 Vol.42 No.3

The purpose of this study was designed to estimate activation of a Malondialdehyde(MDA) and total antioxidant status(TAS) in human by administration the ginseng for eight weeks. For this purpose, K college students(n=7) was to be tested to a treadmill runing from rest until their entire physical exhaustion. Subject`blood was collected before, immediately after, 10 and 30 minutes after the exercise to be examined. The results of this study can be summarized as follows;1. Malondialdehyde(MDA) were significantly lower than before.2. Total antioxidant status(TAS) were significantly higher than before, Particularly TAS(total antioxidant status) were higher than before that in all-out time.In conclusion, the ginseng was decreased that oxydative stress in all-out time. Therefore, Ginseng was effected by Antioxidants.

Serum Antioxidant Vitamins and Erythrocyte Lipid Peroxide Levels in Female Adolescent Smokers

Kim, Jung-Hee,Moon, Jeong-Suk,Lee, Hee-Won Korean Society of Community Nutrition 1999 Journal of community nutrition Vol.1 No.1

The purpose of this study was to investigate the association between adolescent smoking and antioxidant vitamins. Subjects were 87 non-smokers and 90 smokers. who were female high school students. Smokers were divided into tow groups by smoking status, 35 light smokers (packyear<1) and 53 heavy smokers(packyear$\geq$1). Dietary intakes were examined through questionnaires and nutrient intakes of vitamin C and A were analyzed by smoking status using Computer Aided Nutritional analysis program for professional (CAN-PRO). Serum vitamin C level was measured by 2,4-dinitrophenylhydrazine method and serum levels of vitamin A and E were measured by HPLC. Erythrocyte lipid peroxide levels was measured by thiobarbituric acid reactive substance (TBARS) method. All data were statistically analyzed by SAS PC package program. The mean vitamin C intakes of non-smokers, light smokers and heavy smokers were 58.2mg/day, 50.1 mg/day 58.1mg/day, respectively. The mean vitamin A intakes of non-smokers, light and heavy smokers were 28.1$\mu\textrm{g}$R.E./day, 278.7$\mu\textrm{g}$R.E./day and 289.6$\mu\textrm{g}$R.E./day, respectively. There was no significant difference in dietary intakes of antioxidant vitamins by smoking status. However, the serum vitamin C level, 11.40mg/l in heavy smokers was 12% lower than that of than that of 12.70mg/l in non-smokers. The serum vitamin A level was not significantly different among the groups. Vitamin E level, 8.79mg/l in heavy smokers was 8% lower than that of 9.53mg/l in non-smokers. There was no significant correlation between the dietary intakes and serum levels of vitamin A and C. The erythrocyte TBARS level, 1.90nmol/ml in light smokers was significantly lower (p<0.05) than 2.71 nmol/ml in heavy smokers or non-smokers. The correlation data showed that the cerythrocyte TBARS level had a significant positive correlation with packyear. Overall results might suggest that cigarette smokers with a longer smoking history need more dietary intakes of vitamin C and E than do non-smokers to reach the same serum level.

토양수분함량이 지황 ‘토강 ’의 생육 , 광합성 및 항산화효소 활성에 미치는 영향

권아름,장원석,김선익,성봉재,곽성희 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.4

Background: Rehmannia glutinosa, a rhizomatous herbaceous perennial plant, is widely used as a medicinal plant in the Far East region. Although the influence of waterlogging on the growth and yield of R. glutinosa has been evaluated, few studies have focused on how these plants respond to a wide range of soil water status including wet and dry conditions, in terms of growth and physiolog- ical characteristics. Therefore, this study investigated the effect of contrasting soil moisture regimes on the growth, photosynthesis, chlorophyll fluorescence, antioxidant enzyme activity, and bioac- tive compound content in R. glutinosa var. ‘Togang’. Methods and Results: Potted ‘Togang’ plants were irrigated according to five different soil water statuses, −10, −20, −30, −40, and −50 ㎪, with each moisture level controlled using tensiom- eters throughout the experiment. The effects on sprouting rate from rhizome propagules, vegetative growth, rhizome yield, photosynthetic characteristics, antioxidant enzyme activity, and rhizome catalpol content were determined. A significant reduction in the sprouting rate of propagules and vegetative growth (leaf number and growth) was found when plants were irrigated at −50 ㎪. Yield components such as the number of rhizomes and the total and marketable weights of rhizomes were the highest at the −30 ㎪ treatment. Although the total rhizome weight in the −10 ㎪ and −20 ㎪ treatments was as high as that in the -30 ㎪ treatment, the marketable weight was significantly reduced because root rot occurred under these high moisture conditions; root rot decay was the highest at −10 ㎪ (7.6% of total rhizome weight), followed by 5.3% at −20 ㎪, whereas it was neg- ligible in the range of −30 ㎪ and −50 ㎪ soil water status. Regarding water stress-induced antioxi- dant levels, the superoxide dismutase, peroxidase, and catalase activities increased when the plants were stressed with water excess (−10 ㎪) or shortage treatment (−50 ㎪), compared to those with the −30 ㎪ treatment. The net photosynthesis and transpiration rates were considerably higher at −20 ㎪ and −30 ㎪ than those at the other treatments. Chlorophyll fluorescence measurements showed that the −20 ㎪ and −30 ㎪ treatments showed a maximum quantum yield (Fv/Fm) of higher than 0.8 (considered as the stress threshold), whereas the −10 ㎪ or −50 ㎪ treatments showed a quantum yield of less than 0.8. The content of catalpol, a bioactive compound in rhi- zomes, was the highest at −30 ㎪ (35.9 ㎎/g FW). Conclusions: Considering its effect on vegetative growth, yield, some physiological characteristics (photosynthesis and chlorophyll florescence), and rhizome catalpol content, our results indicate that approximately −30 ㎪ of soil water potential is the appropriate soil water status for cultivating Rehmannia glutinosa var. ‘Togang’.

다낭성 난소 증후군 환자에서 Isoflavone 섭취에 따른 혈중 생화학적 지표의 변화

원형재,강주희,이민준,윤선,박기현,조동제,송찬호,이병석,Won, Hyung Jae,Kang, Ju Hee,Lee, Min Jun,Yoon, Sun,Park, Ki Hyun,Cho, Dong Je,Song, Chan Ho,Lee, Byung Seok 대한생식의학회 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.1

Objective: This study was performed to evaluate the effect of isoflavone supplementation on hormone levels, lipid profiles and total antioxidant status in patients with polycystic ovary syndrome. Methods: Total 11 women with polycystic ovary syndrome were supplemented daily with 150mg of isoflavone for 6 months. Blood samples were collected 0, 3, and 6 months after supplement of isoflavone for analysis of LH, FSH, E2, testosterone, free testosterone, SHBG levels, serum lipid profiles, and total antioxidant status (TAS). Results: After 6 months isoflavone supplementation, the hormone levels did not change significantly. Serum lipid profiles did not show any significant change in total cholesterol, LDL-cholesterol, triglyceride, lipoprotein(a), and free fatty acid levels. However, there was significant increase in HDL-cholesterol (p<0.05) for 3 months. Total antioxidant status was increased significantly after isoflavone supplementation for 6 months (p<0.05). Conclusion: Isoflavone supplementation showed positive effects on the HDL-cholesterol and total antioxidant status. it is implicated that isoflavone supplementation will may have a effect on cardiovascular disease in patients with polycystic ovary syndrome.

Effect of Supplemental Selenomethionine on Growth Performance and Serum Antioxidant Status in Taihang Black Goats

Yue, Wenbin,Zhang, Chunxiang,Shi, Liguang,Ren, Youshe,Jiang, Yusuo,Kleemann, D.O. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.3

An experiment was conducted to evaluate the effect of different levels of supplemental selenomethionine (Se-Met) on growth performance and serum antioxidant status in Taihang Black goats. Fifty 16-week-old goats with an average body weight of 12.5${\pm}$0.5 kg were randomly assigned to five treatments fed a basal diet (0.049 mg Se/kg DM) supplemented with 0 (control), 0.10, 0.30, 0.50 and 1.00 mg of Se/kg DM (form Se-Met) for 80 days. Average daily gain and feed efficiency were higher (p<0.05) in the groups supplemented with 0.30 to 0.50 mg Se/kg DM compared with the control group. However, Se-Met supplementation had no influence on average daily feed intake (p>0.05). Se-Met supplementation significantly increased (p<0.01) the activity of glutathione peroxidase enzymes (GSH-Px) and superoxide dismutase (SOD) in serum. The group supplemented with 0.50 mg Se/kg DM had the highest activity of GSH-Px compared with other groups (p<0.05). Serum SOD activity was higher (p<0.05) in goats supplemented with both 0.30 and 0.50 mg Se/kg DM than in control goats and goats supplemented with 1.00 mg Se/kg DM. Serum glutathione-S-transferase (GST) activity and malondialdehyde (MDA) concentration were significantly decreased (p<0.05) in goats supplemented with 0.30, 0.50 and 1.00 mg Se/kg DM compared with control values. These results indicated that Se-Met supplementation markedly improved the antioxidant status in goats. Blood Se concentration increased linearly (p<0.001) and quadratically (p<0.001) as the level of supplemental Se-Met increased. The concentration of Se in the control diet (0.049 mg Se/kg DM) did not satisfy the Se requirement in goats as indicated by reduced growth rate, feed efficiency, activities of GSH-Px and SOD in serum, and blood Se concentrations. In conclusion, it is recommended that 0.30 to 0.50 mg of Se/kg DM from Se-Met (total diet Se of 0.349 to 0.549 mg/kg DM) be supplied in the diet of Taihang Black goats to enhance growth performance and improve antioxidant status.

Antioxidants Supplementation on Acid Base Balance during Heat Stress in Goats

Sivakumar, A.V.N.,Singh, G.,Varshney, V.P. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.11

The effects of vitamin C and vitamin E with selenium on acid-base balance and some stress hormones were evaluated during heat stress in goats. Goats, 1.5 years of age, were divided into control, heat stress and antioxidant treatment groups 1, 2 and 3. Except for the control, all groups were exposed to a temperature of $40{\pm}2^{\circ}C$ with a relative humidity of 30% for 5 h/d for 21 days in a psychrometric chamber. Rectal temperature and respiratory rates were recorded daily post exposure. Blood samples were collected on every 3rd day for estimation of plasma vitamins C and E, total antioxidant activity and hormones, and separate blood samples were taken to estimate acid-base status. The rectal temperature and respiratory rates were increased (p<0.05) in the heat stress group only. Except for pH and $pO_2$, which were increased significantly (p<0.05) other parameters of acid-base balance such as $pCO_2$, $HCO_3^-$, $TCO_2$, BEb, BEcef, PCV and Hb were significantly decreased (p<0.05) in the heat stress group. An improvement in acid-base status was noted in the antioxidant supplemented groups. Prolactin and cortisol levels were significantly (p<0.05) higher and free T3 and T4 levels were significantly (p<0.05) lower in the heat stress group. Levels of prolactin and cortisol were decreased and free T3 and T4 were increased in antioxidant treatment groups. Different levels of antioxidant supplementation resulted in similar protection against heat stress.