Effects of a Single Session of Brain Yoga on Brain-Derived Neurotrophic Factor and Cognitive Short-Term Memory in Men Aged 20-29 Years

( Hyun-seong Yang ),( Hyun-jun Kim ),( Hwa-gyeong Lee ) 대한통합의학회 2021 대한통합의학회지 Vol.9 No.4

Purpose : This study aimed to evaluate the effects of a cognitive enhancement brain yoga program on short-term memory and serum brain-derived neurotrophic factor (BDNF) levels according to the cognitive state in men aged 20-29 years. Methods : Thirty healthy volunteers aged 20-29 years were divided into four groups: brain yoga group, yoga group, combined exercise group, and control group. Seven people were assigned randomly per group. A single-session intervention was conducted over 50 min and consisted of three parts: warm-up, main exercise (brain yoga, yoga, combined exercise, or non-exercise), and cool-down. Serum BDNF levels were measured using enzyme-linked immunosorbent assay, and short-term memory was evaluated using the forward number span test before and after the intervention. Results : BDNF levels significantly increased within the brain yoga group after the intervention (from 28874.37±5185.57 to 34074.80±7321.12, p=.003), whereas there were no significant differences pre-and post-intervention in the other groups. The inter-group comparison showed a significant interaction between the brain yoga group and the combined exercise group (p=.036) but no significant interaction between any of the other groups. Forward number span scores were significantly increased in the brain yoga group (from 9.43±9.83 to 23±7.92, p=.012) and theyoga group after the intervention (from 13.43±9.41 to 24.14±8.45, p=.011), whereas there were no significant changes after the intervention in any other groups. Conclusion : Our findings showed that a single-session, 50-minute brain yoga exercise improved short-term memory and increased serum BDNF levels in healthy men aged 20-29 years and that yoga improved only short-term memory in healthy men of this age group.

The association of disease activity, pro-inflammatory cytokines, and neurotrophic factors with depression in patients with rheumatoid arthritis

Cheon, Yun-Hong,Lee, Seung-Geun,Kim, Mingyo,Kim, Hyun-Ok,Sun Suh, Young,Park, Ki-Soo,Kim, Rock Bum,Yang, Hyun-Su,Kim, Ji-Min,Son, Chang-Nam,Kyoung Park, Eun,Kim, Sang-Hyon,Lee, Sang-Il Elsevier 2018 Brain, behavior, and immunity Vol.73 No.-

<P><B>Abstract</B></P> <P>Inflammation and trophic factors (brain-derived neurotrophic factor [BDNF], vascular endothelial growth factor, glial cell line-derived neurotrophic factor, and insulin-like growth factor-1) are associated with depression in the general population. Rheumatoid arthritis (RA) is a chronic representative inflammatory autoimmune disease; however, the association of disease activity, pro-inflammatory cytokines, and neurotrophic factors with depression has not been sufficiently investigated. Therefore, we determined the prevalence of depression and risk factors for depression and deterioration of depressive symptoms in RA patients. In addition, we analyzed the association between disease activity, pro-inflammatory cytokines, trophic factors, and depression in RA (N = 474). Demographic and laboratory data were examined, and routine assessment of patient index data 3 (RAPID 3) and disease activity score 28-joint count C-reactive protein (DAS 28-CRP) was performed to assess disease activity of RA. Depression was measured using the Korean version of the Beck Depression Inventory-second edition (K-BDI II). A K-BDI score ≥18 was considered the cut-off for depression in accordance with a previous validation study. The serum level of pro-inflammatory cytokines and neurotrophic factors was assessed by enzyme-linked immune sorbent assay. The prevalence of depression was 32.4% in patients with RA. The severity of disease activity of RA (RAPID 3 score [OR 2.34; 95% confidence interval, CI 1.22–4.51], DAS 28-CRP [≥3.2] [OR 1.60, 95% CI 1.01–2.53]) and severity of fatigue (OR 1.26 95% CI 1.15–1.38) were associated with depression and deterioration of depressive symptoms in the multivariate analysis. Among the components of RAPID 3 and DAS 28-CRP, patient assessment for global health and abilities for daily performance were more related to depression. The level of pro-inflammatory cytokines (IL-1β, IL-6, TNF-alpha) was not related to depression. The level of BDNF was significantly lower in RA patients with depression and was negatively correlated with K-BDI II score. Depression was related with the level of fatigue, low expression of BDNF, and high RA disease activity, which was associated with impaired ability to perform activities of daily life. Strict control of fatigue and disease activity to improve one’s capacity to perform daily life activities would be important to regulate depression. The level of BDNF might be one of the possible biomarkers to predict or monitor depression in patients with RA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Prevalence of depression in patients with rheumatoid arthritis (RA) was analyzed. </LI> <LI> Risk factors for depression and deterioration of depressive symptoms and were analyzed. </LI> <LI> Depression was related with disease activity in RA. </LI> <LI> Depression in RA was associated with impaired ability to perform activities of daily life. </LI> <LI> Among neurotrophic factors, the level of BDNF was related with depression in RA patients. </LI> <LI> The level of pro-inflammatory cytokines were not related with depression in RA patients. </LI> </UL> </P>

Colchicine 처리 후 흰쥐 뇌에서 Brain-Derived Neurophic Factor 면역 반응 신경세포의 분포

김정기,조희중,박원학 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.6

Colchicine 처리후 흰쥐 뇌에서 Brain-Derived Neurotrophic Factor 면역반응 신경세포의 분포

김정기,박원학,조희중 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

Brain-derived neurotrophic factor(BDNF) mRNA 및 BDNF는 뇌에서 광범위하게 분포함이 밝혀져 있다.BDNF mRNA와 BDNF는 그 분포에 있어서 서로 일치하는 부위가 많지만 그렇지 않은 부위도 있다. 본 연구에서는 BDNF mRNA는 발현되지만 BDNF가 발현되지 않는 부위에서 BDNF의 발현을 보기 위하여 뇌실에 colchicine을 투여한 후 면역조직화학기법을 시행한 결과 다음의 사실을 알 수 있었다. 1.앞후각핵, 조롱박겉질, 대되겉질,담장,CA2와 CA3의 피라밋충, 치아이랑의 과립세포층, 바닥가쪽 편도핵, 가쪽무릎핵, 앞안쪽시상핵,뇌실곁시상핵,뇌실곁시상하부핵 및 배쪽안쪽시상하부핵에서는 colchicine비처리군에 비하여 BDNF면역반응 신경세포가 증가하였다. 2. CA1의 피라밋충,A1,A2,A4-A10세포군, 큰솔기핵, 가쪽거대세포핵 및 척수안뜰신경핵에서는 colchicine처리 후 새로이 많은 BDNF면역반응 신경세포가 관찰되었다. 이상의 결과는 colchicine처리 후 흰쥐 뇌에서 BDNF함유 신경세포들은 그 분포에 있어서 BDNF mRNA함유 신경세포와 일치함을 알 수 있었다. Widespread brain-derived neurotrophic factor(BDNF) mRNA and protein expression has been detected in the brain.Despite substantial overlap between BDNF mRNA and protein expression, there is general anatomical regions, where there is discordance of these expression.We performed, therefore, immunohistochemistry after colchicine treatment into the ventricle to evaluate the possible presence of BDNF-immunoreactive(IR)in the regions where BDNF mRNA was expressed, but not BDNF-IR . The results obstained were as folloes; There was substantial increase in the number of BDNF-IR neurons in the anterior olfactory nucleus, the piriform cortex, the cerebral cortex, the claustrum, the stratum pyramidate of the CA2 and the CA3, the granule cell layer of the dentate gyrus, the basolateral amygdaloid nucleus, the lateral geniculate nucleus, the anteromedial thalmic nucleus,the anterodorsal thalamic nucleus, the paraventricular thalamic nucleus, the paraventricular hypothalamic nucleus and the ventromedial hypothalamic nucleus, compared to the same brain area of non-colchicine treated rat.We detected many new BDNF-IR neurons in the stratum pyramidale of the CA1, A1, A2,A4-A10 cell groups, C1-C3 cell groups, the raphe magnus nucleus, the lateral paragigantocellular nucleus and the spinal vestibular nucleus. The results show that the localization of BDNF-IR neurons after colchicine treatment is consistant with that of BDNF mRNA containing neurons in the brain.

모래쥐 대뇌의 일과성 허혈로 인한 뇌유도 신경영양성 인자(Brain-derived Neurgtrophic Factor)의 유전자 발현 변화

박민규,고성범,박건우,이대희 대한신경과학회 1998 대한신경과학회지 Vol.16 No.5

Colchicine 처리후 흰쥐 뇌에서 Brain-Derived Neurotrophic Factor 면역반응 신경세포의 분포

김정기(Jeong-Ki Kim),박원학(Won-Hark Park),조희중(Hee-Jung Cho) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.6

Brain-derived neurotrophic factor (BDNF) mRNA 및 BDNF는 뇌에서 광범위하게 분포함이 밝혀져 있다. BDNF mRNA와 BDNF는 그 분포에 있어서 서로 일치하는 부위가 많지만 그렇지 않은 부위도 많다. 본 연구에서는 BDNF mRNA는 발현되 지만 BDNF가 발현되지 않는 부위에서 BDNF의 발현을 보기 위하여 뇌실에 colchicine을 투여한 후 면역조직화학기법을 시 행한 결과 다음의 사실들을 알 수 있었다. 1. 앞후각핵, 조롱박겉질, 대뇌겉질, 담장, CA2와 CA3의 피라밋층, 치아이랑의 과립세포층, 바닥가쪽편도핵, 가쪽무릎핵, 앞 안쪽시상핵, 앞등쪽시상핵, 뇌실곁시상핵, 뇌실곁시상하부핵 및 배쪽안쪽시상하부핵에서는 colchicine 비처리군에 비하여 BDNF 면역반응 신경세포가 증가하였다. 2. CA1의 피라밋층, A1, A2, A4-A10 세포군, C1-C3 세포군, 큰솔기핵, 가쪽거대세포핵 및 척수안뜰신경핵에서는 colchicine 처리 후 새로이 많은 BDNF 면역반응 신경세포가 관찰되었다. 이상의 결과는 colchicine 처리 후 흰쥐 뇌에서 BDNF 함유 신경세포들은 그 분포에 있어서 BDNF mRNA 함유 신경세포 와 일치함을 알 수 있었다. Widespread brain-derived neurotrophic factor (BDNF) mRNA and protein expression has been detected in the brain. Despite substantial overlap between BDNF mRNA and protein expression, there is general anatomical regions, where there is discordance of these expression. We performed, therefore, immunohistochemistry after colchicine treatment into the ventricle to evaluate the possible presence of BDNF-immunoreactive (IR) in the regions where BDNF mRNA was expressed, but not BDNF-IR. The results obtained were as follows; There was substantial increase in the number of BDNF-IR neurons in the anterior olfactory nucleus, the piriform cortex, the cerebral cortex, the claustrum, the stratum pyramidale of the CA2 and the CA3, the granule cell layer of the dentate gyrus, the basolateral amygdaloid nucleus, the lateral geniculate nucleus, the anteromedial thalamic nucleus, the anterodorsal thalamic nucleus, the paraventricular thalamic nucleus, the paraventricular hypothalamic nucleus and the ventromedial hypothalamus nucleus, compared to the same brain area of non-colchicine treated rat. We detected many new BDNF-IR neurons in the stratum pyramidale of the CA1, A1, A2, A4-A10 cell groups, C1-C3 cell groups, the raphe magnus nucleus, the lateral paragigantocellular nucleus and the spinal vestibular nucleus. The results show that the localization of BDNF-IR neurons after colchicine treatment is consistant with that of BDNF mRNA containing neurons in the brain.

말초염증에 의한 등족뿌리신경절의 brain-derived neurotrophic factor 합성의 변화

김상열,김정기,김효신,이상지,하선옥,홍해숙,김동선 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

Differential stimulation of neurotrophin release by the biocompatible nano-material (carbon nanotube) in primary cultured neurons

Kim, Yun Gi,Kim, Jong Wan,Pyeon, Hee Jang,Hyun, Jung Keun,Hwang, Ji-Young,Choi, Seong-Jun,Lee, Ja-Yeon,Deá,k, Ferenc,Kim, Hae-Won,Lee, Young Il SAGE Publications 2014 Journal of biomaterials applications Vol.28 No.5

<P>In order to develop novel, effective therapies for central nervous system regeneration, it is essential to better understand the role of neurotrophic factors and to design, accordingly, better artificial scaffolds to support both neurite outgrowth and synapse formation. Both nerve growth factor and brain-derived neurotrophic factor are major factors in neural survival, development, synaptogenesis, and synaptic connectivity of primary cultured neurons. As a prime candidate coating material for such neural cultures, carbon nanotubes offer unique structural, mechanical, and electrical properties. In this study, carbon nanotubes coated glass-coverslips were used as the matrix of a primary neural culture system used to investigate the effects of carbon nanotubes on neurite outgrowth and nerve growth factor/brain-derived neurotrophic factor release and expression. For these purposes, we performed comparative analyses of primary cultured neurons on carbon nanotubes coated, non-coated, and Matrigel-coated coverslips. The morphological findings showed definite carbon nanotubes effects on the neurite outgrowths and synaptogenic figures in both cortical and hippocampal neurons when compared with the non-coated negative control. Although the carbon nanotubes did not change neurotrophin expression levels, it stimulated brain-derived neurotrophic factor release into the media from both types of neurons. Accordingly, we suggest a different mechanism of action between carbon nanotubes and Matrigel in relation to the specific neurotrophic factors. Since carbon nanotubes supply long-term extracellular molecular cues for the survival and neurite outgrowths of cultured neurons, the results from this study will contribute to an understanding of carbon nanotubes biological effects and provide new insight into their role in the secretion of neurotrophic factors.</P>

열성경련과 열성경련플러스(GEFS+)에서 Brain-derived neurotrophic factor(BDNF) SNP 6265의 다형성

김상은(Sang Eun Kim),김현규(Hyon Gyu Kim),김영훈(Young Hoon Kim),최병준(Byung Joon Choi),황희승(Hee Seong Hwang),빈중현(Joong Hyun Bin),정승연(Seung Yun Chung),이인구(In Goo Lee) 대한소아신경학회 2008 대한소아신경학회지 Vol.16 No.2

목 적 : 열성경련은 영유아에서 가장 흔한 신경학적 질환 중 하나이나 아직 그 병인은 분명하게 밝혀 지지 않았다. 최근 많은 연구에서 Brain-derived neurotrophic factor(BDNF)의 발현이 신경세포의 흥분을 증가시킨다고 보고되고 있다. 이에 저자들은 BDNF 유전자의 SNP 6265 다형성이 열성경련의 감수성을 예측하는 유용한 표지자인지 여부를 알아보기 위하여 열성경련 환아 및 이전에 연구되지 않았던 열성경련플러스 환아를 대상으로 BDNF 유전자의 SNP 6265 다형성의 빈도를 정상 대조군과 비교, 분석하였다. 방 법: 가톨릭대학교 소아과를 방문한 총 79명을 대상으로 열성경련 환아 30명, 열성경련플러스 환아 19명, 그리고 건강한 정상 대조군 30명의 세 군으로 분류하였으며, BDNF 유전자의 SNP 6265 다형성 여부를 확인하기 위해 각 군간 유전자형 및 대립유전자 빈도를 비교하였다. 결 과 : 세 군의 유전자형 분포는 AA 동형접합자, A/G 이형접합자 및 GG 동형접합자가 열성경련 환아군에서 46.7%, 36.7% 및 16.7%, 열성경련 플러스 환아군에서 26.3%, 47.4% 및 26.3%, 정상 대조군에서는 60.0%, 16.7% 및 23.3%로 가장 흔한 유전자형은 열성경련 환아군과 정상 대조군에서는 A/A 동형 접합자인 반면 열성경련 플러스 환아군에서는 A/G 이형접합자였다. 그리고 BDNF 유전자에 대한 A와 G의 대립유전자 빈도를 분석한 결과는 열성경련 환아군에서 65.0%와 35.0%였고 열성경련플러스 환아군에서 50%와 50%였으며 정상 대조군에서는 68.3%와 31.7%로 열성경련 환아군과 정상 대조군에서 A 대립유전자의 빈도가 더 높았다. 하지만 이러한 유전자형 분포와 대립 유전자 빈도상의 차이는 통계적 의의는 없었다. 결 론: 저자들은 BDNF 유전자의 SNP 6265 다형성이 열성경련 및 열성경련플러스의 병인과 관련이 없으며 이들 질환에 대한 감수성을 예측하는 유용한 표지자가 아님을 확인하였다. Purpose : Febrile seizure(FS) is one of the most common neurological conditions during childhood, but the pathogenesis of FS remains ambiguous. Various studies have shown that brain-derived neurotrophic factor(BDNF) increased neuronal excitability. In this study, to determine whether the polymorphisms of SNP 6265 within the gene encoding BDNF are associated with susceptibility to FS, the frequencies of the polymorphisms were investigated in children with FS and control subjects. In addition, we analyzed the SNP 6265 polymorphisms in Generalized epilepsy with febrile seizures plus (GEFS+) that hasn't been studied as yet in Korea. Methods : A total of 79 children selected throughout a collaborative study of Catholic Child Neurology Research Group were divided into three groups: (1) FS(n=30); (2) GEFS+ (n=19); (3) control subjects(n=30). Genotypes and allelic frequencies for the polymorphisms of SNP 6265 located at nucleotide 196 was analyzed and compared among the groups. Results : In this study, proportions for A homozygote, A/G heterozygote and G homozygote for BDNF were as follows: in FS, 46.7%, 36.7% and 16.7%, in GEFS+, 26.3%, 47.4% and 26.3% and in control subjects, 60.0%, 16.7% and 23.3%. The allele A and G frequencies for BDNF in FS were 65.0% and 35.0%, in GEFS+ were 50% and 50%, and in control subjects were 68.3% and 31.7%. However, these differences in genotype proportions and allele frequencies among three groups were not significant. Conclusion : These results suggest that genomic variations of BDNF might not be the susceptibility factor for FS and GEFS+ in Korean population.

Effects of γ-aminobutyric acid-enriched fermented sea tangle (Laminaria japonica) on brain derived neurotrophic factor-related muscle growth and lipolysis in middle aged women

최욱철,Storm N. S. Reid,유제광,김윤숙,조영홍,전병환 한국조류학회I 2016 ALGAE Vol.31 No.2

This study evaluated the effects of γ-aminobutyric acid (GABA)-enriched fermented sea tangle (GFST), as a functional food, on brain derived neurotrophic factor (BDNF)-related muscle growth and lipolysis, in a sarcopenic obesity high-risk group. Twenty-one middle-aged women (53-63 y) participated in this randomized, double-blind, placebo controlled study. Participants ingested either 1,000 mg of GFST (n = 10) or a sucrose placebo (CON) (n = 11) everyday, for 8 weeks. Subjects were asked to abstain from any regular exercise. Fasting venous blood samples, body composition and muscular strength were measured before and after supplementation period. Collectively, we demonstrated that GFST significantly decreased total fat mass and triglyceride in body composition, as well as significantly increasing serum BDNF (p < 0.001), angiotensin converting enzyme (p < 0.001), human growth hormone and insulin-like growth factor-1 levels (p < 0.05 and p < 0.05, respectively) accompanied by increased total lean mass (p < 0.01). Furthermore, the reported improvements in total work, knee extension and flexion at 60° s-1 (p < 0.05), and peak torque normalized to body weight of knee flexion at 60° s-1 (p < 0.05), support an ergogenic effect of GABA associated with increased growth factor levels. The use of GFST, as a functional food ingredient, to elicit anti-obesity effects and stimulate the release of muscle-related growth factors with increasing serum BDNF levels may provide a protective intervention for age-related degeneration such as sarcopenic obesity.