Fabrication and Characterization of Silver Nanoparticle and its Potential Antibacterial Activity

P. Prema,Rincy Raju 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6

In the present research silver nanoparticle was fabricated by chemical reduction of silver salt (Silver nitrate, AgNO3) solution. Sodium citrate was used as a reducer. The formation of silver nanoparticle was observed visually by color change (greenish yellow). The surface plasmon resonance peak in absorption spectra of silver nanoparticle showed an absorption maximum at 420 nm in UV-VIS spectrometry. The X-ray diffraction pattern showed the presence of sharp reflections at 111, 200, 220, and 311. This would indicate the presence of silver nanoparticle. The scanning electron micrograph revealed that the average size of silver nanoparticle was 21.22 ± 5.17 nm. Silver nanoparticle exhibited better antimicrobial activity against Staphy lococcus aureus than the other bacterial pathogens. The correlation coefficient between silver nanoparticles and selected bacterial pathogens revealed that there is a strong negative correlation with Escherichia coli, S.aureus and Klebsiella pneumonia (r = −0.975, −0.993, and −0.998, respectively). In the present research silver nanoparticle was fabricated by chemical reduction of silver salt (Silver nitrate, AgNO3) solution. Sodium citrate was used as a reducer. The formation of silver nanoparticle was observed visually by color change (greenish yellow). The surface plasmon resonance peak in absorption spectra of silver nanoparticle showed an absorption maximum at 420 nm in UV-VIS spectrometry. The X-ray diffraction pattern showed the presence of sharp reflections at 111, 200, 220, and 311. This would indicate the presence of silver nanoparticle. The scanning electron micrograph revealed that the average size of silver nanoparticle was 21.22 ± 5.17 nm. Silver nanoparticle exhibited better antimicrobial activity against Staphy lococcus aureus than the other bacterial pathogens. The correlation coefficient between silver nanoparticles and selected bacterial pathogens revealed that there is a strong negative correlation with Escherichia coli, S.aureus and Klebsiella pneumonia (r = −0.975, −0.993, and −0.998, respectively).

금속나노입자-유전체 이층 구조 구현을 위한 반투명 Cu 나노입자층 형성에 관한 연구

윤혜련,조윤이,윤회진,이승윤,Yoon, Hye Ryeon,Jo, Yoon Ee,Yoon, Hoi Jin,Lee, Seung-Yun 한국전기전자재료학회 2020 전기전자재료학회논문지 Vol.33 No.6

This study reports the fabrication and application of semitransparent Cu nanoparticle layers. Spin coating and subsequent drying of a Cu colloid solution were performed to deposit Cu nanoparticle layers onto Si and glass substrates. As the spin speed of the spin coating increases, the density of the nanoparticles on the substrate decreases, and the agglomeration of nanoparticles is suppressed. This microstructural variation affects the optical properties of the nanoparticle layers. The transmittance and reflectance of the Cu nanoparticle layers increase with increasing spin speed, which results from the trade-off between the exposed substrate area and surface coverage of the Cu nanoparticles. Since the glass substrates coated with Cu nanoparticle layers are semitransparent and colored, it is anticipated that the application of a Cu nanoparticle-dielectric bilayer structure to transparent solar cells will improve the cell efficiency as well as aesthetic appearance.

Nanoparticle Ferrite Multilayers Prepared by New Self-Assembling Sequential Adsorption Method

Kim, Yeong-Il,Kang, Ho-Jun,Kim, Don,Lee, Choong-Sub Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.5

The nanoparticle magnetite of which diameter was about 3 nm was synthesized in a homogeneous aqueous solution without a template. The synthesized magnetite nanoparticle was easily oxidized to maghemite in an ambient condition. The magnetic properties of the ferrite nanoparticle show superparamagnetism at room temperature and its blocking temperature is around 93 K. Modifying the sequential adsorption method of metal bisphosphonate, we have prepared a multilayer thin film of the ferrite nanoparticle on planar substrates such as glass, quartz and Si wafer. In this multilayer the ferrite nanoparticle layer and an alkylbisphosphonate layer are alternately placed on the substrates by simple immersion in the solutions of the ferrite nanoparticle and 1, 10-decanediylbis (phosphonic acid) (DBPA), alternately. This is the first example, as far as we know, of nanoparticle/alkyl-bisphosphonate multilayer which is an analogy of metal bisphosphonate multilayer. UV-visible absorption and infrared reflection-absorption studies show that the growth of each layer is very systematic and the film is considerably optically transparent to visible light of 400-700 nm. Atomic force microscopic images of the film show that the surface morphology of the film follows that of the substrate in μm-scale image and the nanoparticle-terminated surface is differentiated from the DBPA-terminated one in nm-scale image. The magnetic properties of this ferrite/DBPA thin film are almost the same as those of the ferrite nanoparticle powder only.

Effect of biocompatible polymers on the physicochemical and dissolution properties of fenofibrate in nanoparticle system

최종학,이기범,Sungho Hong,Seok Kyu Lee,오유경,Seung Kyu Choi,최한곤 한국약제학회 2013 Journal of Pharmaceutical Investigation Vol.43 No.6

This study aimed to evaluate the effect of biocompatible polymers on the physicochemical and dissolution properties of poorly water-soluble drugs in nanoparticle systems. Four types of nanoparticles containing poorly watersoluble fenofibrate were prepared using solvent evaporation technique with different biocompatible polymers such as polyvinylpyrrolidone (PVP), hydroxypropylmethyl cellulose (HPMC), carbopol and ethylcellulose. Their physicochemical properties were investigated using scanning electron microscopy, differential scanning calorimetry, and powder X-ray diffraction. The solubility and dissolution of nanoparticle-entrapped fenofibrate were compared with those of free drug powder. Biocompatible polymers affected the morphology and sizes of fenofibrate nanoparticles. PVP or carbopol-based nanoparticles showed spherical appearance,whereas HPMC or ethylcellulose-based nanoparticles formed aggregates with irregular shape. The particle sizes increased in the order of the nanoparticle prepared with carbopol B PVP\HPMC\ethylcellulose. The size of PVP-based nanoparticles did not significantly differ fromthat of carbopol-based nanoparticles, showing the mean sizes of ca. 10 lm. As compared to free drug powder, the solubility and dissolution of the drug in nanoparticles increased in the order of PVP[HPMC[carbopol[ethylcellulose. The enhanced solubility and dissolution of poorly water-soluble fenofibrate via nanoparticle system did not depend on particle size but on crystallinity. In conclusion, in nanoparticle development of poorly water-soluble drugs such as fenofibrate,the nature of biocompatible polymers plays an important role in the physicochemical and dissolution of poorly water-soluble drugs in the nanoparticles.

Nanoparticle-protein complexes mimicking corona formation in ocular environment

Jo, D.H.,Kim, J.H.,Son, J.G.,Dan, K.S.,Song, S.H.,Lee, T.G.,Kim, J.H. IPC Science and Technology Press 2016 Biomaterials Vol.109 No.-

Nanoparticles adsorb biomolecules to form corona upon entering the biological environment. In this study, tissue-specific corona formation is provided as a way of controlling protein interaction with nanoparticles in vivo. In the vitreous, the composition of the corona was determined by the electrostatic and hydrophobic properties of the associated proteins, regardless of the material (gold and silica) or size (20- and 100-nm diameter) of the nanoparticles. To control protein adsorption, we pre-incubate 20-nm gold nanoparticles with 5 selectively enriched proteins from the corona, formed in the vitreous, to produce nanoparticle-protein complexes. Compared to bare nanoparticles, nanoparticle-protein complexes demonstrate improved binding to vascular endothelial growth factor (VEGF) in the vitreous. Furthermore, nanoparticle-protein complexes retain in vitro anti-angiogenic properties of bare nanoparticles. In particular, priming the nanoparticles (gold and silica) with tissue-specific corona proteins allows nanoparticle-protein complexes to exert better in vivo therapeutic effects by higher binding to VEGF than bare nanoparticles. These results suggest that controlled corona formation that mimics in vivo processes may be useful in the therapeutic use of nanomaterials in local environment.

Gene expression profiling associated with treatment of positive charged colloidal silica nanoparticle in human neuroblastoma cells

김지희,박희옥,장석원,신차균,류재천,김연정,양성익 한국바이오칩학회 2011 BioChip Journal Vol.5 No.4

Among the physico-chemical properties of nanoparticles, surface chemistry is one of the most important factors in cytotoxicity or cellular injurious effects of nanoparticles. However, the role of specific surface chemistry involved in the intracellular responses is not well understood. We investigated the comprehensive gene expression profile of biological responses of commercially available colloidal silica nanoparticles with different charge in human neuronal cell line, SHSY5Y. Positive charged silica CL nanoparticle showed less cytotoxicity than negative charged silica AM at more than 200 ppm. At 100 ppm, however, both positive and negative charged silica nanoparticles gave rise to low cytotoxicity. On the other hand, transcriptional changes by these silica nanoparticles represented that positive charged silica CL affected to much more genes than negative charged silica AM. Through gene ontology (GO) analysis, we identified that positive charged silica CL nanoparticle affected to the receptor mediated cellular responses, organization of cytoskeleton like as actin and regulation of transcription by ribosomal proteins or transcription factors. Unlike to negative charged silica nanoparticles, positive charged silica CL nanoparticle did not affect to sterol metabolism and lipid transport. Especially, some cell junction related genes (CTNNA1, PTPRM, CLDN19 and CTNNA1) were differentially expressed by positive charged silica nanoparticle, and these genes may be involved in interaction between the positive charged nanoparticle and molecules with negative charge present in cellular membrane. Thus, we suggest that specific surface charge of silica nanoparticle may be an important point being considered when assesses the safety of silica nanoparticles in neuronal cells.

Neurotoxic effects by silica TM nanoparticle is independent of differentiation of SH-SY5Y cells

Youn Jung Kim,양성익 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.4

Silica nanoparticles are being formulated for potential drug delivery and for imaging and diagnostic applications in the central nervous system (CNS). However, the potential and underlying mechanism of silica nanoparticles-mediated neurotoxicity has remained unclear. We examined the effect of colloidal silica nanoparticle (LUDOX® TM) on neurite outgrowth and neurotoxicity of human SH-SY5Y neuroblastoma cells differentiated by all-trans-retinoic acid (RA). Exposure of ¤300 ppm of SiO2 TM nanoparticle in differentiating cells showed less cytotoxicity than in undifferentiated cells. 100 ppm of SiO2 TM nanoparticle had no significant difference on the viability of either undifferentiated or differentiating SH-SY5Y cells. Neurite outgrowth in differentiating cells for 48 h exposure of 100 ppm SiO2 TM nanoparticle was not significantly changed. Thus, SiO2 TM nanoparticle appeared no effects in the early initiation of neurites. And also,although the production of reactive oxygen species (ROS) was not induced, neurotoxicity resulted by SiO2 TM nanoparticle may be the result of increased DNA damage, apoptosis, and cell cycle arrest in undifferentiated and differentiating cells. Further studies are needed to investigate the expression of genes in these signaling pathways in response to exposure to silica nanoparticle and to investigate the molecular mechanisms of neuronal cell effects.

저온에서 Hydropolymer를 이용한 ZnO 나노입자 염료 감응형 태양전지

권병욱,손동익,박동희,양정도,최원국,Kwon, Byoung-Wook,Son, Dong-Ick,Park, Dong-Hee,Yang, Jeong-Do,Choi, Won-Kook 한국재료학회 2010 한국재료학회지 Vol.20 No.9

To fabricate $TiO_2$ nanoparticle-based dye sensitized solar cells (DSSCs) at a low-temperature, DSSCs were fabricated using hydropolymer and ZnO nanoparticles composites for the electron transport layer around a low-temperature ($200^{\circ}C$). ZnO nanoparticle with 20 nm and 60 nm diameter were used and Pt was deposited as a counter electrode on ITO/glass using an RF magnetron sputtering. We investigate the effect of ZnO nanoparticle concentration in hydropolymer and ZnO nanoparticle solution on the photoconversion performance of the low temperature fabricated ($200^{\circ}C$) DSSCs. Using cis-bis(isothiocyanato)bis(2,20 bipyridy1-4,40 dicarboxylato) ruthenium (II) bis-tetrabutylammonium (N719) dye as a sensitizer, the corresponding device performance and photo-physical characteristics are investigated through conventional physical characterization techniques. The effect of thickness of the ZnO photoelectrode and the morphology of the ZnO nanoparticles with the variations of hydropolymer to ZnO ratio on the photoconversion performance are also investigated. The morphology of the ZnO layer after sintering was examined using a field emission scanning electron microscope (FE-SEM). 60 nm ZnO nanoparticle DSSCs showed an incident photon-to-current conversion efficiency (IPCE) value of about 7% higher than that of 20 nm ZnO nanoparticle DSSCs. The maximum parameters of the short circuit current density ($J_{sc}$), the open circuit potential ($V_{oc}$), fill factor (ff), and efficiency ($\eta$) in the 60 nm ZnO nanoparticle-based DSSC devices were 4.93 mA/$cm^2$, 0.56V, 0.40, and 1.12%, respectively.

Movement of Metal Nanoparticle and GNP mediated Photothermal Impact in Arabidopsis

구연종 ( Yeonjong Koo ),자넷부라암 ( Janetbraam ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-

The basic information to develop nanoparticle as a functional material is discovering nanoparticle movement in plant tissues. Two different methods were carried out to tracking nanoparticle in Arabidopsis. First, Arabidopsis was exposed to CdSe/CdZnS QDs with three different coatings, anionic, cationic, and relatively neutral, and nanoparticle movement was dramatically changed from the particle absorption into the plant root cell to the particle localization in plant leaves. T. ni caterpillars that fed on Arabidopsis exposed to QDs had reduced performance, and QD fluorescence was detected in both T. ni bodies and frass, demonstrating trophic transfer of intact QDs from plants to insects. Second, gold nanoparticle was applied to test the biological responses to photothermal effects of nanoparticles. The uptake of gold nanoparticle through Arabidopsis roots and translocation to leaves are reported through the photoacoustic signal detection. Furthermore, Arabidopsis leaves harboring GNPs and exposed to continuous laser or noncoherent light show elevated temperatures across the leaf surface and induced expression of heat-shock regulated genes. Overall, these results demonstrate that metal based nanoparticles are strong candidate as a substance carrier for manipulating plant physiology.

Optimal Shape Design of the Film-Coupled Nanoparticle using the Phase Field Design Method

이학용,성홍경,유정훈 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.4

Localized surface plasmon resonance (LSPR) occurs when an electromagnetic (EM) wave hits a metal nanoparticle. The interaction between a gold (Au) nanoparticle and a thin metal film produces a strong EM wave called as LSPR at the small gap between the nanoparticle and the film. The field strength of the LSPR increases dramatically as the distance between the Au nanoparticle and the film decreases. In this study, we focus on the field enhancement at the small gap by obtaining the appropriate shape of the Au nanoparticle. Since the shape or the size of a nanoparticle to enhance the LSPR is hard to be determined theoretically, the structural optimization method based on the phase field method is employed to design the shape of the nanoparticle. To obtain reliable results taking the small gap of 2 nm into account, we proposed a new filtering scheme based on a smoothed Heaviside function and applied it to nanoparticle design.