간흡충 cystein proteinase 유전자의 클로닝

박현,홍경만 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Clonorchis sinensis is one of the most important parasitic trematode in China, Korea, Japan and Southeast Asia. When intermediate host eat raw fish which is infected with it metacercariae, the worm excyst in gut. migrate along bile duct and grow up to adult worm. Recently, cysteine proteinases in trematoda parasites were reported to be specific antigens which reacted with the sera of the infected patients. In our preliminary data, the cysteine proteinase in Clonorchis sinensis also reacted with the sera of Clonorchiasis patients. So we think that the cysteine protinase of C. sinensis could be possible candidate as an antigen for the serodiagnosis of Clonorchiasis patients. Therefore, we cloned cysteine proteinase cDNA to find out the cysteine proteinase which could react with the sera of Clonorchiasis patients. In this study, we cloned a cysteine proteinase gene from adult C. sinensis. And also we compared the expression of the cysteine proteinase between C. sinensis and P. westermani, and then were inspected to a specificity of that gene. Cysteine proteinase cDNA fragment from adult Clonorchis sinensis was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using degenerate oligonucleotide primers derived from conserved cysteine proteinase sequences. The amplified yielded a cDNA fragment of approximately 500 bp in length, the amplified product was directly cloned into a T- vector and was sequenced. Sequence analysis and aligment showed significant similarity to other eukaryotic cysteine proteinases and conservation of the cysteine, histidine, and asparagine residues that formed the catalytic triad. To study the cross reactivity with the sera of the patients of Paragonimiasis, we did PCR with C. sinensis and P. westermani cDNA Library. It was only amplified in cysteine of C. sinensis. These results indicated that the cysteine proteinase gene of C. sinensis might be used as an antigen forbe for diagnosis.

Immunoglobulin and Cytokine Production from Mesenteric Lymph Node Lymphocytes Is Regulated by Extracts of Cordyceps sinensis in C57Bl/6N Mice

Dong Ki Park,Wahn Soo Choi,Pyo-Jam Park,Eun Kyung Kim,Yong Jun Jeong,Se Young Choi,Koji Yamada,Jong Dai Kim,임병우 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.4

Cordyceps sinensis, one of the well-known fungi used in traditional Chinese medicine, is recognized to play a role in the metabolic process of inflammation and immunity. The purpose of this study was to evaluate the effects of water extracts of C. sinensis on the immune function of mesenteric lymph node (MLN) lymphocytes in C57Bl/6N mice. C. sinensis-treated mice were administered the respective extract by oral gavage for 4 weeks. Immunoglobulin E concentrations in serum and MLN lymphocytes were significantly lower in C. sinensis-treated mice than in control mice. In contrast, the immunoglobulin A concentration from the C. sinensis group was higher than that in control mice. C. sinensis increased the proportion of CD4+ and CD8+ T cells in MLN lymphocytes. C. sinensis significantly decreased interleukin-4 and interleukin-10 cytokine concentrations. Therefore, water extracts of C. sinensis modulate immune parameters through regulation of immunoglobulin production resulting from decreased T-lymphocyte helper 2 cytokine secretion and reduce cytokine secretion in MLN lymphocytes.

Angelica sinensis induces hair regrowth via the inhibition of apoptosis signaling.

Kim, Mi Hye,Choi, You Yeon,Cho, Ik-Hyun,Hong, Jongki,Kim, Sung-Hoon,Yang, Woong Mo Institute for Advanced Research in Asian Science a 2014 The American journal of Chinese medicine Vol.42 No.4

<P>Hair loss is accompanied by keratinocyte apoptosis-regression during catagen and prolonged telogen. Angelica sinensis was reported to promote hair growth in vitro. Based on previous studies, we explored the hair growth effect and the mechanism of A. sinensis related to keratinocyte apoptosis-regression during catagen in mice. The 70% Ethanol extract of A. sinensis was applied topically at doses of 1 and 100 mg/mL to the dorsa of C57BL/6 mice for 2 weeks. The A. sinensis-treated group showed noticeable hair regrowth. Treatment with A. sinensis restored the lengths of hair shafts and size of hair follicles. In addition, mice treated with A. sinensis showed notably decreased apoptotic cells, along with a significant change in the expression of cleaved caspase-3 and the ratio of a pair of apoptosis-associated proteins: Bcl-2 and Bax. Also, A. sinensis inhibited the nuclear translocation of NF-κB, the phosphorylation of IκB-α, the phosphorylation of three mitogen-activated protein MAP kinases, and the activation of c-Jun with decreased TNF-α. These findings reveal a role of A. sinensis as an alternative treatment for hair loss that acts through hair cycle pathways associated with apoptosis regression during catagen.</P>

Phylogenetic Analysis of Native Vigna sinensis in Korea Using DNA Sequence of Internal Transcribed spacer (ITS) Region

Pil-Soo Seo(서필수),Sook-Young Lee(이숙영),Yong Kook Shin(신용국) 한국생명과학회 2017 생명과학회지 Vol.27 No.3

본 연구에서 밝혀진 갓끈동부의 ITS1, 5.8S 및 ITS2의 염기서열은 NCBI (National Center for Biotechnology Information)의 GenBank에 Vigna sinensis AY195581로 등록하였다. ITS1, 5.8S 및 ITS2의 총 염기서열 507 염기서열을 이용한 Vigna sinensis (AY195581)의 분자계통분석에서 Vigna unguiculata 및 그 아종들과 98~100% 범위의 염기서열 상동성을 보였다. Vigna unguiculata는 계통분석에 이용된 다른 종들로부터 독립된 하나의 cluster로 그룹핑(grouping)이 됨을 확인하였다. 본 계통분석은 Vigna unguiculata가 Vigna 속의 다른 종에 비해 비교적 최근에 분화되었으며, 현재 유전적인 변화가 많이 일어나고 있음 보이고 있다. 또한, Vigna 속, Vigna longifolia, Vigna vexillata, Vigna membranacea, Vigna friesiorum, Vigna monophylla, Vigna schimperi, Vigna nigritia, Vigna lasiocarpa, Vigna trichocarpa, Vigna diffusa의 다른 종들과 비교하여 유전적으로 독립적인 종임을 확인하였다. 본 연구의 Vigna sinensis의 ITS1, 5.8S 및 ITS2를 이용한 계통분석은 Vigna sinensis를 Vigna unguiculata로 분류하는 것이 타당한 것으로 보여진다. 본 종은 국내에서 멸종된 것으로 알려져 있었으나 최근 토착 식물로써 발견되었고 이 갓끈동부의 관련 식물 종들과의 분자계통학적 위치를 명확히 밝힘에 의의가 있다고 하겠다. Cowpea (Vigna unguiculata (L.) Walp.) is recognized as a potential source of protein and other nutrients. The genus Vigna includes 100 wild species of plants. Especially, Vigna unguiculata includes annual cowpeas (ssp. unguiculata) and ten wild perennial subspecies. DNA sequence of internal transcribed spacer (ITS) region was determined for Vigna sinensis, one of native plant, which was found in recent but thought to have gone extinct in Korea. The seeds of Vigna sinensis used in this study were donated from Dong-Young Jo. The DNA sequence of ITS-5.8S-ITS2 for Vigna sinensis obtained from this study was deposited as Vigna sinensis AY195581 on GenBank of NCBI (National Center for Biotechnology Information). We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. DNA similarities among subspecies including Vigna unguiculata showed the range 98 to 100% in sequence-based phylogenetic analysis using total 507 base pairs of ITS1, 5.8S and ITS2. Vigna unguiculata and subspecies were grouped independently as one cluster from other Vigna species used in the phylogenetic analysis. In this study, based on the phylogenetic analysis using the ITS1-5.8S-ITS2 sequence of Vigna sinensis, it may be concluded to be classified to one of Vigna unguiculata substrains.

멸종위기어류 가시고기 Pungitius sinensis (Pisces: Gasterosteidae)의 분포현황 및 멸종위협 등급 평가

고명훈 ( Myeong-hun Ko ) 한국어류학회 2016 韓國魚類學會誌 Vol.28 No.3

우리나라 멸종위기어류 가시고기 Pungitius sinensis의 분포양상 및 멸종위협 등급을 평가하기 위하여 2015년 3월부터 10월까지 강원도와 경상북도 등에서 정밀조사를 실시하였다. 조사 결과 71개 조사지점 중 21개 하천 (지점)에서 854개체가 채집되었다. 과거 30여 년 동안 가시고기는 23개 하천에서 서식이 확인되었으나 이 지점들에 대한 본 조사 결과 12개 하천에서만 서식이 확인되는 등 서식지 및 개체수가 급격히 감소하였다. 가시고기의 서식지 특징은 하천 하류의 소 (pool)가 형성되고 수생식물이 많이 서식하는 곳이었다. IUCN 적색목록 기준지침서에 따라 멸종위협 등급을 평가하면, 서식지 및 개체 수의 급격한 감소와 작은 점유 면적 (84 km²) 등으로 위기 (EN) [B2ab(ii,iii,iv,v)]로 평가되었다. Distribution status and threatened assessment of endangered species, Pungitius sinensis (Pisces: Gasterosteidae), were investigated from March to October 2015 in Gangwon-do and Gyeongsangbuk-do etc., Korea. During the study period, a total of 854 individual P. sinensis were collected from 21 of 71 sampling stations using skimming nets. P. sinensis appeared in 23 streams during the past 30 years. However, it was only collected in 12 streams. Therefore, its habitats and populations were drastically reduced. The preferred habitat of P. sinensis was pool of the lower stream with lots of aquatic plants. Given these evidences of small occupying areas (84 km²) and drastic reduction in its habitats and populations, P. sinensis is considered as an endangered (EN) [B2ab(ii,iii,iv,v)] species based on IUCN Red List categories and criteria.

당귀(Angelica sinensis)의 임상활용에 대한 효능 및 부작용연구

박영철,이지선,김민희,김동윤,이선동,Park, Yeong-Chul,Lee, Ji-Sun,Kim, Min-Hee,Kim, Dong-Yoon,Lee, Sun-Dong 대한한의학방제학회 2011 大韓韓醫學方劑學會誌 Vol.19 No.2

Objectives : Angelica sinensis has been used extensively in Korean traditional medicine. Although thorough clinical trials are lacking, Various pharmacological actions for Angelica sinensis has been identified newly using animal models. In addition, it was reported that reactive intermediates, potentially causing toxic effects, was isolated from components in Angelica sinensis. In this article, it was purposed for explanation and introduction of new studies for Angelica sinensis. in terms of Pharmacological action and Toxicity. Methods : New studies for Angelica sinensis were reviewed and summarized in terms of pharmacological action and toxicity. Results and Conclusions : Angelica sinensis and its components including phthalide, phthalide dimers, polysaccharides, polyacetylenes, ligustilide와 butylidenephthalide as well as organic acid showed a variety of pharmacological actions on uterine, cardiovascular system and immune system. In addition, it was identified that three components of such as ligustilide, caffeic acid and safrole was biotransformed to reactive intermediates causing possibly toxicity.

A Real-Time qPCR Assay to Quantify Ophiocordyceps sinensis Biomass in Thitarodes Larvae

Wei Lei,Shaosong Li,Qingyun Peng,Guren Zhang,Xin Liu 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

Ophiocordyceps sinensis, an entomogenous fungus parasitic in the larvae of moths (Lepidoptera), is one of the most valuable medicinal fungi, and it only distributed naturally on the Tibetan Plateau. The parasitical amount of O. sinensis in various tissues of the host Thitarodes larvae has an important role in study the occurrence and developmental mechanisms of O. sinensis, but there no an effective method to detect the fungal anamorph. A real-time quantitative PCR (qPCR) system, including a pair of species-specific ITS primers and its related program, was developed for O. sinensis assay with high reliability and efficiency. A calibration curve was established and exhibited a very good linear correlation between the fungal biomass and the CT values (R2=0.999419)by the qPCR system. Based on this method, O. sinensis was detected rapidly in four tissues of its host caterpillars, and the results were shown as following: the maximum content of O. sinensis parasitized in the fat-body, and next came bodywall;both of them were much larger than that observed in the haemolymph and intestinal-wall. Taken together, these results show that qPCR assays may become useful tools for study on developmental mechanism of O. sinensis.

경상남도 일부 농촌지역의 간흡충 감염에 대한 역학적 연구

주영희,오진경,공현주,손운목,김윤규,김정일,정갑열,신해림,Ju, Young-Hee,Oh, Jin-Kyoung,Kong, Hyun-Joo,Sohn, Woon-Mok,Kim, Yoon-Gyu,Kim, Jung-Il,Jung, Kap-Yeol,Shin, Hai-Rim 대한예방의학회 2005 Journal of Preventive Medicine and Public Health Vol.38 No.4

Objectives : To investigate the prevalence of Clonorchis Sinensis infestation and to determine the associated risk factors among a population in a part of the rural area of Gyeongsangnam-do, Korea, which is an area known to have a high mortality from liver cancer and a high infection of C. sinensis. Methods : The study populat ion consisted of those people who have lived in rural areas and who were over 40 years old. This study was performed in 5 areas during the period from 1999 to 200 3. Informed consents were obtained from the 2,381 study participants, and these people were interviewed about their life style habits with using the structured questionnaire that was administered by trained staff members. The subjects underwent blood sampling and their stool specimens were examined by using the Formalin-ether sedimentation technique. Results : Among the study subjects (N=2,381), the positivity for C. sinensis in the stool was 34.4% (95% CI=36.3-42.5); it was 39.4% (95% CI=36.3-42.5) in the males and 30.9% (95% CI=28.5-33.3) in the females . The positivity for C. sinensis was associated with current alcohol drinking (odds ratio=1.8, 95% CI: 1.5-2.1) and raw fish consumption (odds ratio 1.2, 95% CI: 0.9-1.6). Conclusion : The present study confirmed the high prevalence of C. sinensis in the study subjects. It is necessary to establish consistent medical management and education programs for the treatment and prevention of C. sinensis infestation in the rural inhabitants of Korea.

급성 담낭염과 급성 담관염을 보인 간디스토마 감염증

양유미,최한림,류동희,우창곡,한정호,박선미 대한췌담도학회 2019 대한췌담도학회지 Vol.24 No.2

59세 여자가 복부 통증을 주소로 내원하였다. 복부 전산화 단층촬영에서 담관 결석이 의심되는 병변이 발견되었다. 내시경역행담췌관조영술을 하는 중에 간디스토마 성충이 배출되었다. 대변과 혈청에서 간디스토마 충란과 항체가 각각 발견되어 간디스토마 감염증으로 진단하였다. 내시경 시술과 구충제를 복용 후 증상은 서서히 호전되었다. 환자는 간디스토마 비유행지역에 거주하였고 날 것이나 덜 익힌 민물생선을 섭취한 기왕력이 없었다. 대한민국은 간디스토마 유행지역에 속하며 사람들은 조리기구 등을 통한 간접 접촉을 통해서 감염될 수 있다. 그러므로 대한민국에서 담관 질환이 있는 환자들을 진료할 때 간디스토마 감염의 가능성을 고려하여야 한다. 우리는 문헌고찰과 함께 임상 증례를 보고한다. A 59-year-old woman presented with abdominal pain. Abdominal computerized tomography was suggestive of biliary stones. During endoscopic retrograde cholangiopancreatography, adult worms resembling Clonorchis sinensis (C. sinensis) were drained. Eggs were detected in stool using the formalin-ether concentration method and C. sinensis-specific antibody was detected in the serum. A diagnosis of C. sinensis infection was made. The symptoms of the patient gradually resolved after treatment with anti-parasite medication. The patient lived in a non-endemic region for C. sinensis infection and had no history of intake of raw or undercooked freshwater fishes. South Korea is one of the endemic countries for C. sinensis infection and people can be infected via indirect routes of transmission such as cooking utensils. Therefore, the possibility of C. sinensis infection should be considered in patients presenting with biliary diseases in South Korea. We describe the clinical findings of this case with a review of literature.

다엽의 5가지 페놀성분과 R-(-)-carvone의 치아우식균 Mutans Streptococci에 대한 항균력 상승효과

송옥희 ( Ok-hee Song ),강옥화 ( Ok-hua Kang ),문수현 ( Su-hyun Mun ),김민철 ( Min-chul Kim ),한영선 ( Young-sun Han ),최성훈 ( Sung-hoon Choi ),이영섭 ( Young-seob Lee ),권동렬 ( Dong-yeul Kwon ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.5

Objectives : Camellia sinensis (Theaceae) possesses a various beneficial effects such as free radical-scavenging, inactivation of urokinase in cancer cell proliferation, antibacterial, and hypotensive. Dental caries is one of the most common oral infectious disease in a human. Oral microorganisms play a significant role in the etiology of dental caries. An aberration to this ecology due to dietary habits, improper oral hygiene or systemic factors lead to an increase in cariogenic microorganisms. Cariogenic microorganisms like Streptococcus mutans and Streptococcus sobrinus encourage the accumulation and adherence of plaque biofilm by metabolizing sucrose into glucans. The purpose of this study was to investigate the antimicrobial activity of phenolic compounds of Camellia sinensis and R-carvone, monoterpenes, is can be found naturally in numerous essential oils, on Streptococcus mutans and Streptococcus sobrinus . Methods : The antimicrobial activity of these compounds was determined by the broth microdilution method and checkerboard dilution assay to investigate the potential synergistic effects of each five compounds of Camellia sinensis (C. sinensis) and R-carvone. Results : C. sinensis-isolated compounds and R-carvone were determined with MIC of more than 1,000 ㎍/mL. However, the combination test showed significant synergism against S. mutans and S. sobrinus , implicated in the lowered MICs. Conclusions : These results suggest that combinatory application of phenolic five compounds (theophyllin, l-theanine, epicatechin, epicatechin gallate, and caffeine) from C. sinensis and R-carvone has a potential synergistic effect and thus may be useful as a mouthrinse in helping control cariogenic microorganism.