Increased expression of P53 and Bax in the spinal cords of rats with experimental autoimmune encephalomylitis

문창종,김승준,위명복,김희석,정종태,박전홍,지영흔,Tanumab, Naoyuki,Matsumoto, Yoh,신태균 濟州大學校 農科大學 動物科學硏究所 2000 動物科學論叢 Vol.15 No.1

The expression of pro-apoptotic molecules, p53 and Bax, in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE) was examined. Apoptosis was confirmed by the terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling (TUNEL) method. TUSEL (+) apoptotic cells were mainly either ED1 (+) macrophages or T-cells in the parenchyma of E N . Western blot analysis showed that both p53 and Bax expression significantly ( p < 0.01) increased in the spinal cords of EAE rats at the peak stage, and thereafter declined. An immunohistochemical study showed that inflammatory cells (notably T cells) in the parenchyma express p53 and Bax, while brain cells, includng neurons and glia, were devoid of these nuclear staining of these molecules. The nuclear expression of p53 largely matches apoptotic cells in the parenchyma of EAE. These finchngs suggest that pro-apoptotic molecules, p53 and Bax, may play an important role in eliminating T cells in the parenchyma in EAE.

제주 차귀도 연안해역 미생물 분포 및 이화학적 특성

문영건,김만철,이준백,송춘복,여인규,김종만,박근태,손홍주,최우봉,허문수 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3

베타, 감마-불포화 케톤의 광화학에 미치는 히드록실기의 영향 : o-Hydroxyphenyl 1,2-Dimethylcyclopent-2-enyl Ketone의 합성 Synthesis of o-Hydroxyphenyl 1,2-Dimethylcyclopent-2-enyl Ketone

문종택,공영철,주동준 慶熙大學校 1997 論文集 Vol.26 No.-

To study the effect of the intramolecular hydrogen bondinghydroxyl group on the photochemistry of a β,γ-unsaturated ketone, racemic ο-hydroxyphenyl 1,2-dimethylcyclopent-2-enyl ketone and ο-methoxyphenyl 1,2-dimethylcyclopent-2-cnyl ketone were synthesized. Thus these two β,γ-unsaturated ketone were synthesized from ethyl 2-oxocyclopentanecarboxylate, after eight steps, in 18% yield.

양어용 사료첨가제로서 감귤발효액 (EM-Fermented Orange)의 항산화특성

문상욱,이영돈,이준백,고유봉 제주대학교 해양연구소 2000 해양자원연구소연구보고 Vol.24 No.-

Mandarine orange (Citrus unshiu Marc.) only added with sugar was fermented by effective microorganisms (EM). called "EM-fermented orange". EM was mainly composed of lactic acid bacteria. phototrophic bacteria and yeast. As pH of EM-fermented orange reached 3.5 to 4.0 in the course of fermentation. the incubation was stopped. and then. EM-fermented orange was preservable at room temperature for about 6 months. Composition and concentration of free amino acids in EM-fermented orange were analyzed. Concentration of total amino acids was 167 mg/l. and the relative content of carnosine (β-alanyl-L-histidine) which was well known as a kind of antioxidant materials was very high. 41%. In addition. crude extract showing antioxidant activity could be obtained from EM-fermented orange using some organic solvents. The antioxidant activity of the crude extract was equivalent to about 9% of that of vitamin E by the use of 1.1-diphenyl-2-picrylhydrazyl (DPPH) method. It was suggested that EM-fermented orange having antioxidant characteristics could promote the health of fish.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

쥐와 개의 부신의 Somatostatin 분포에 대한 면역조직화학적 연구

문정석,정윤영,김준수 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

The adrenal medulla using immunohistochemistry few somatostatin immunoreactive nerve fibers were demonstrated in adrenal medulla, In addition, a large population of chromattin cells in the dog adrenal medulla displayed intense somatostatin-like immunoreactivity especially in the peripheral and perivascular region. About 10~20% of the adrenaline cells were the somatostatin-immunoreactive. The present study confirms the somatostatin is localized in the dog adrenal chromattin cell, and it is suggested that somatostatin may have a paracrine or endocrine role in the dog adrenal gland.

사람의 비후성 반흔에서 면역조직화학기법에 의한 형질전환인자 알파와 표피성장인자 발현의 차이에 관한 면역조직화학적 연구

박영란,김현곤,김종중,문정석,송준섭,설경수,정윤영 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background and Objectives: Transforming growth factor-α (TGF-α) and epidermal growth factor (EGF) are polypeptides which interact with the epidermal growth factor receptor (EGFR) to produce their biological effects. The aim of the present investigation was to elucidate the immunolocalization of TGF-α and EGF in normal human skin, hypertrophic scar with skin graft on soft tissue defect, and postburn hypertrophic scar without graft. Methods: The data presented in this paper focused attention on differences of expression between two kinds of hypertrophic scars in relation to skin graft using immunohistochemistry. Formalin-fixed and paraffin-embedded tissues from 3 normal skin tissues, 3 hypertrophic scars after skin graft and 8 postburn hypertrophic scars without graft were immunolabelled with antibodies directed against TGF-α and EGF. Results: In normal epidermis of skin, strong TGF-α immunoreactivity (IR) was observed in all epidermal layers except the stratum (S.) basale, whereas EGF was immunopositive in a few cells over all epidermal layers. The staining for TGF-α was found in cell membrane, and EGF was seen either diffuse cytoplasmic or peripheral part of cells in normal epidermis. In hypertrophic scar after six months postoperation, TGF-α IR was observed in the upper part of S. spinosum and S. corneum, whereas EGF was diffusely expressed in the S. spinosum, also its intensity was slightly increased compared to normal skin. In hypertrophic scar without skin graft, very strong expression of EGF was detected in the S. spinosum and S. corneum, and intensity of EGF was increased when compared to normal skin and postoperative hypertrophic scar. EGF and TGF-α coexisted in the cells of S. spinosum in normal skin as well as hypertrophic scars. Double-labeled cells were increased in postburn hypertrophic scar compared to normal skin and postoperative hypertrophic scar. In addition, intensity of EGF and TGF-α double IR was the strongest in postburn hypertrophic scar. Conclusion: These results indicate that EGF may have a more complex regulatory role not only in the early stages of wound healing but also in hypertrophic scar.

흰쥐의 척수에 분포하는 Substance P 양성 신경섬유에 관한 면역조직화학적 연구

김영준,정윤영,김종중,문정석,정주현 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

An immunohistochemical study was performed using the specific monoclonal antibody against substance P to demonstrate the substance P immunoreactive nerve fibers in the spinal cord of the rats. 1. In the rat spinal cord using immunofluorence method, substance P immunoreactive cell bodies were not found, but their fibers with beaded appearance were observed in all spinal segments. 2. SP immunoreactive nerve fibers were found with the highest concentration especially in the lumbosacral segments. 3. SP immunoreactive nerve fibers could be seen in the Lamina Ⅰ,Ⅱ, X and a great number of substance P immunoreactive nerve fibers appeared in the IML. 4. A smaller number of nerve fibers were observed in the Lamina Ⅸ of ventral region and in the white matter.

高層아파트 團地의 形狀要素에 관한 硏究(Ⅰ) : 小規模 高層아파트 團地의 駐車空間을 中心으로 Centering on the parking place in small-scale high-rise apartment building complex

朴文瑀,姜大奎,趙容準 조선대학교 동력자원연구소 1991 動力資源硏究所誌 Vol.13 No.1

All dwelling complex should be plan with the consideration on the cars. this is because the car is no only a necessary tool for the man's various wants, but also a checking cause for the dwelling complex's comforts. this study focuses on the apartment complex of the dwelling scale taking a large percentage in Kwangju, and the various matters and the remedy about the parking space. the results are following. 1. The parking space is affected by the dwelling bwelling's arrangement, the open space and the incidental facilities. therefore the apartment complex plan of the future must apply the view of mass space. 2. Recently, the environs of the dwelling building are used as a parking space. It affects a bad effect on the complex's comfort. So the laws enacted to establish the underground parking place are required. 3. For the great comforts and closeness, the complex's plan has to consider the closeness of the complex entrance, the dwelling building entrance and the parking space.

양어용 사료첨가제로서 감귤발효액(EM-Fermented Orange)의 항산화특성

이준백,문상욱,이영돈,고유봉 제주대학교 해양연구소 2000 해양과환경연구소 연구논문집 Vol.24 No.-

Mandarine orange (Citrus unshiu Marc.) only added with sugar was fermented by effective microorganisms (EM) called "EM-fermented orange". EM was mainly composed of lactic acid bacteria, phototrophic bacteria and feast. As pH of EM-fermented orange reached 3.5 to 4.0 in the course of fermentation, the incubation was stopped. and then. EM-fermented orange was preservable at room temperature for about 6 months. Composition and concentration of free amino acids in EM-fermented orange were analyzed. Concentration of total amino acids was 167mg/l, and the relative content of carnosine (β-alanyl-L-histidine) which was well known as a kind of antioxidant materials was very high. 41%. In addition. crude extract showing antioxidant activity could be obtained from EM-fermented orange using some organic solvents. The antioxidant activity of the crude extract was equivalent to about 9% of that of vitamin E by the use of 1.1-diphenyl-2-picrylhydrazyl (DPPH) method. It was suggested that EM-fermented orange having antioxidant characteristics could promote the health of fish.