Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

팥의 RAPD 분석 조건 최적화 연구

이충열,박현철,박진철,김성만,김용철,최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The object of this study was to optimize PCR condition for RAPD analysis in adzuki bean. The best template DNA concentration was 20ng(0.25unit taq polymerase and 2.5mM MgCl2), 40ng(1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2 and lunit taq polymerase and 7.0mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2) The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), 4.5mM(20ng template DNA and 0.25unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(40ng template DNA and 1unit taq polymerase). Amount of taq polymerase was 0.25unit(20ng template DNA and 2.5mM MgCl2), 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, the best condition for PCR optimization was 60ng template DNA, 4.5mM MgCl2, and 1unit taq polymerase. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

화염을 내포하는 영상의 검출에 대한 연구

도용태,최병수 대구대학교 정보통신연구소 2012 情報通信硏究 Vol.9 No.1

화재는 여러 가지 재난들 중에서 가장 흔하다. 따라서 이에 대한 효과적인 대책이 필요하지만, 기존의 연기 검출기를 비롯한 단순한 형태의 센서들은 자주 오작동하는 문제가 있다. 이 문제에 대한 해결책으로 카메라의 영상을 사용하여 화재를 검출하는 방법에 대한 관심이 근래 증대되고 있다. 본 논문에서는 화염을 포함하는 영상을 검출하기 위한 기법이 서술된다. 주어진 영상을 다수의 영역으로 나눈 후, 각 영역의 특성으로부터 화염의 존재 유무를 판정한다. 서술된 기법은 다양한 영상들을 이용하여 그 성능을 평가하였으며, 그 성공률은 약 73%이었다. 이와 같은 결과는 연속 영상에 의한 화재감지에 사용되어 더 높은 성공률로 개선될 수 있을 것이다. detection using camera images. In this paper, a technique to detect images containing flames is described. After segmenting a given image into multiple regions, the existence of flames is determined from the characteristics of each region. The described technique was tested using various images, and we could get about 73% of success rate. This result may be improved by detecting fire in image sequences.

유기 능동 소자 제작을 위한 유기 박막의 제작 및 전기적 특성

崔淙宣,김대엽,李榕洙 弘益大學校 科學基術硏究所 1999 科學技術硏究論文集 Vol.10 No.2

There are currently considerable interest in the applications of conjugated polymers, oligomers, and small molecules for thin-film electronic devices. Organic materials have potential advantages to be utilized as semiconductors in field-effect transistors and light-emitting diodes. In this study, pentacene thin-film transistors (TFTs) were fabricated on glass substrate. Aluminums were used for gate electrodes. silicon dioxide was deposited as a gate insulator by PECVD and patterned by reactive ion etching (R.I.E). Gold was used for the electrodes of source and drain. The active semiconductor pentacene layer was thermally evaporated in vacuum at a pressure of about 10?? Torr and a deposition rate 0.3Å/s. The fabricated devices exhibited the field-effect mobility as large as 0.07㎠/V.s and on/off current ratio as larger than 10??.

동양 전통과학의 탐구방법론 연구 : 朱子의 格物致知論을 중심으로

최영제,이우붕,정원우,김용수,문장수,방경곤,김기정 경북대학교사범대학과학교육연구소 2005 科學敎育硏究誌 Vol.29 No.-

MPLS망에서 RSVP와 DiffServ연동모델의 성능분석

최승권,오구영,지홍일,신승수,조용환 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.1

The explosive growth of the internet traffic has imposed tremendous stress on routers, particularly, in the core network. Today's IP services reflect the unpredictable and undifferentiated packet loss and jitter characteristics of traditional best-effort routers. For above requirements, it is required not only the high-speed transmission of the packet, but the new routing function, the guarantees of QoS and bandwidth, the supports of user-requested services MPLS is proposed by the IETF and many other research institute as the solutions for this problem. In this paper, analyze performance of QoS Model applied RSVP over DiffServ to MPLS network. this model is each RSVP applied access network and DiffServ applied core network. In Result, It is amiss that basis MPLS net uses to core network because the average packet loss rate and average delay time are increased if traffic increases. Experiment result was seldom difference in case of network that apply each RSVP, DiffServ, RSVP over DiffServ. RSVP over DiffServs case packet loss rate and delay time were low than DiffServ and the packet loss rate is high because comparing than RSVP but delay time was very low.

증류식 소주 제조 중 고급 알콜류의 생성

최진상,이용수 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

백미원료와 대맥원료의 일반성분은 큰 차이가 없었지만, 전분가는 백미원료가 대맥원료보다 3.4% 정도 높았다. 백미원료와 대맥원료의 알콜 수율은 백미원료가 우수하였으며, 대맥원료는 산의 생성량이 많았다. 효모별 발효율은 위스키 효모인 Saccharomyces cerevisiae Suntory균이 가장 높았고, 다음으로 Sake, Mikada 및 Hansen 균의 순이었다. 원료별 고급 알콜류로서 n-propanol, iso-butanol 및 iso-amyl alcohol 등을 본석하였으며, 고급 알콜류의 생성량은 백미원료가 대맥원료보다 높았으나 생성 특성에는 큰 차이가 없었다. 효모종류별 고급 알콜류의 생성특성은 유사하였지만, 그 생성량에서는 약간의 차이가 있었다. General compositions were not difference between rice and barley, bur carbohydrate value was higher in rice than barley as above 3.4%. Alcohol ratio was better in rice than barley, but barley had higher contents in the formation of acids. Fermentation rate of yeast varieties vas best in Saccahromyces cerevisiae Suntory and the next order was Sake, Mikada and Hansen species. Higher alcohols were more in rice than in barley, but the characteristic of formation was not difference. And the formation of yeast varieties had little difference between species but the characteristic of formation was similar to four species.

복합재료 구조물 수리시 발생하는 잔류변형에 대한 덧대기 현상

최항석,이수용 한국항공대학교 1999 論文集 Vol.37 No.-

복합재료 구조물의 덧대기 수리시에 발생하는 잔류변형에 대한 덧대기의 영향을 파악하기 위해서 유한요소 해석을 수행하였다. 유한요쇼 프로그램은 삼차원 응축 쉘 요소와 일차 전단변형 이론에 근거하여 개발되었다. 준등방성, 직교이방성, angle-ply 적층 쉘에 대해 각각 angle-ply, 준등방성, 직교이방성 덧대기 적층으로 수리하였을 때 최종 잔류변형률을 계산하였다. 최대 잔류변형률은 대부분 적층쉘과 덧대기의 인접 부위에서 발생하였고, 그 부위에서 상당히 급격하게 변화하였다. 잔류변형은 적층쉘과 덧대기의 적층순서에 따라 크게 좌우되며, 또한 cutout 영역이 커짐에 따라 일반적으로 잔류변형이 증가하였다. Finite element analysis is performed to investigate the effect of patches on residual deformation induced by the patch repair of composite structures. The finite element program is developed based upon a 3-D degenerated shell element and the first shear deformation theory. Final residual strains are calculated for quasi-isotropic, orthotropic and angle-ply laminated shells repaired with angle-ply, quasi-isotropic and orthotropic prepreg patches, respectively. Maximum residual strains are occurred and abruptly changed near at interfaces of the laminated shell and the patch. Residual deformation is depended on stacking sequences of the laminated shell and the patch, and is generally increased as the region of a cutout is increased.

ASYMPTOTIC BEHAVIOR OF BETTI NUMBERS

CHOI, SANGKI,LEE, YONG-SOO 建國大學校 敎育硏究所 2003 論文集 Vol.27 No.-

Artinian 국소환(R, m)에서 S. Choi가 개발한 판별도구를 이용하여 R=k[[X_1,…X_d]]/I꼴의 artinian 국소환에서 Betti Numbers 수열이 지수적으로 증가하는 국소환 R의 유형을 I 에 따라 다음과 같이 구하였다. (a) I가 X_1…X_d 를 제외한 차수가 d 인 모든monomial 들로 생성된 homogeneous ideal 일 때 (b) I가U^d_i=^1X_1…X^_i…X_d 를 제외한 차수가 d-1 인 모든 monomial들로 생성된 homogeneous ideal 일 때