Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

Effect of treadmill exercise on skeletal muscle autophagy in rats with obesity induced by a high-fat diet Do Keun Cho1

( Do Keun Cho ),( Dong Hun Choi ),( Joon Yong Cho ) 한국운동영양학회 2017 Physical Activity and Nutrition (Phys Act Nutr) Vol.21 No.3

[Purpose] This study aimed to investigate the effects of treadmill exercise on body weight, blood biochemistry, and autophagy. [Methods] Triglyceride, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, insulin, and glucose levels were measured, the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) score was calculated, and the soleus muscle was analyzed for autophagy- related factors (Beclin-1, p62, LC3, Lamp- 2) in rats with obesity induced by a high-fat diet. Eight-week-old Sprague Dawley rats were fed a high-fat diet for 35 weeks and then subjected to 10 weeks of treadmill exercise. The experimental group was divided into a Normal Diet-Sedentary (ND-SED, n=8) group, an (High-Fat Diet-Sedentary (HF-SED, n=8) group, and an High-Fat Diet + Treadmill Exercise (HF-TE, n=8) group. The intensity of treadmill exercise was set as 8 m/min for 5 min, 11 m/min for 5 min, 15 m/min for 20 min, and 11 m/min for the last 5 minutes. A glucose tolerance test was performed at the 2nd and 8th week of exercise by sampling of tail blood. [Results] With endurance exercise, the HFTE group showed a significant decrease in body weight, with improved blood biochemical indices and HOMA-IR scores, in comparison with the HF-SED group. However, there was no significant difference in Beclin-1, p62, LC3, and Lamp-2 proteins as measured by autophagic flux in the soleus muscle. [Conclusion] Treadmill exercise induced improvements in body weight, body fat, and biochemical indicators of obesity and Type 2 diabetes, but had no effect on autophagy in soleus muscle.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

植物凝集素 Celosia argentea 와 赤血球에 의한 사람 唾液의 型分類에 關한 硏究

趙容寬,黃迪駿 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.1

Phytagglutinins were utilized for the purpose of blood grouping and also are studying for the grouping of other human contents, eg. : semen. saliva, and urine. In order to classify the human saliva, hemagglutination inhibition tests were performed using a phytagglutinin, Celosia argentea, and 300 human saliva specimens. The Celosia argentea extracts, which was absorbed by the human saliva, could be grouped as inhibitor or non-inhibitor according to agglutination reaction upon the human red blood cells and were compared with the established saliva groupings, blood groups, and serum types statistically. The results obtained were summarized as follows : 1. Human saliva could be classified into two groups. One group inhibited the agglutination activity of Celosia argentea extracts to human red blood cells and the other did not. Among the investigated 300 human saliva specimens, the frequency of the former was 27% and the latter was 73%. 2. The classification and distribution of this saliva types by Celosia argentea showed no statistically significant relation with those of the other known saliva types, such as secretor and non-secretor. 3. The classification and distribution of this sailva types by Celosia argentea showed no statistically significant relation with those of the other known blood groups, such as ABO, MN, H and CI, respectively. 4. The classification and distribution of thls saliva types bY Celosia argentea showed no statistically signiflcant relation with those of the other known serum types, such as CAI,CAI-C,CAI-D,IPO,ICO, respectively. 5. The classification of this saliva types by Celosia argentea showed no statistically significant relation with those of the other known saliva types by Agastache rugosa and Sophola japonica.

용융염산화 반응기에서 기체체류량 및 기-액 흐름특성

조용준,양희철,은희철,유재형,김준형 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.5

용융염산화는 혼성폐기물과 유해폐기물을 효과적으로 산화 및 분해할 수 있는 소각대체기술중 하나이다. 본 연구에서는 직경이 0.079 m이고 높이가 0.653 m인 용융염산화 반응기에서 기체체류량 및 기-액 흐름특성에 대한 연구를 수행하였다. 액상으로 용융탄산나트륨을 기상으로 건조된 공기를 사용하였으며 기체유속(0.05-0.22 m/s)과 용융염온도(870-970℃) 변화가 기체체류량 및 기-액 흐름특성에 미치는 영향을 규명하였다. 기체체류량은 용융염 온도가 상승함에 따라 증가하였는데, 이는 용융염 온도의 증가로 인해 용융된 탄산나트륨의 점도와 표면장력이 감소하였기 때문이다. 실험에서 얻어진 기체체류량 데이터를 drift-flux 모델에 적용하여 용융염반응기 내의 흐름특성을 규명할 수 있었으며, 이를 통해 흐름영역에 따른 기체체류량을 정확하게 예측할 수 있었다. Molten salt oxidation is one of the most promising alternatives to incineration that can be used to efficiently destroy the organic components of mixed wastes and hazardous wastes. In this study, the gas holdup and gas-liquid flow characteristics are investigated in the molten salt oxidation reactor (0.076 m D×0.653 m H.). Effects of input air velocity (0.05-0.22m/s ) and molten salt temperature (870-970℃) on the gas holdup and flow characteristics have been studied. Molten carbonate as the liquid phase and air as the gas phase have been used in this study. The gas holdup increases with increasing molten salt temperature due to the decrease of viscosity and surface tension of molten carbonate. The experimentally obtained gas holdups in the molten salt reactor have been well described and characterized by means of drift-flux model. The gas holdups with variation of the flow regime have been well predicted.

[논문]도시하수처리장으로부터 분리한 호기성 탈질균의 특성

정용주,조순자,이상준 부산대학교 환경문제연구소 2004 環境硏究報 Vol.22 No.-

Characterization of an Aerobic Denitrifier Isolated from Municipal Sewage Treatment Plant

리마틸 정(부시라민 100㎎)에 대한 부시린 정의 생물학적 동등성

조혜영,이문석,오인준,김동현,문재동,이용복 한국약제학회 2001 Journal of Pharmaceutical Investigation Vol.31 No.2

Bucillamine is a novel cysteine derivative with two free intramolecular sulfhydryl groups, and has a preventive and therapeutic effect on adjuvant arthritis, suggesting its antirheumatic action. With respect to the effect on the immune system, bucillamine-exerted such immunoregulating actions are to nomalize an excessive reduction or acceleration in immune reaction. It is useful not only in patients with early stage of rheumatoid arthritis (RA) but also in those with active RA retained for more than 10 years. The purpose of the present study was to evaluate the bioequivalence of two bucillamine tablets, Rimatil^TM (Chong Kun Dang Pharmaceutical Co., Ltd.) and Bucilin^TM (Kuhn Il Pharmaceutical Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). Eighteen normal male volunteers, 23.67±2.09 years in age and 65.03±6.73 kg in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After three tablets containing 100 mg of bucillamine per tablet were orally administered, blood was taken at predetermined time intervals and the concentrations of bucillamine in serum were determined using GC/MS with mass selective detector. Pharmacokinetic parameters such as AUC_t, C_max, and T_max were calculated and ANOVA test was utilized for the statistical analysis of the parameters. The results showed that the differences in AUC_t, C_max and T_max between two tablets were -0.29%, -3.20% and 8.22%, respectively, when calculated against the Rimatil^TM tablet. The powers (1-β) for AUC_t and C_max were 84.31 % and 91.16%, respectively. Minimum detectable differences (Δ) at α=0.10 and 1-β=0.8 were less than 20% (e.g., 18.58% and 16.51% for AUC_t and C_max respectively). The 90% confidence intervals were within ±20% (e.g., -12.77∼12.20 for AUC_t and -14.30∼7.90 for C_max). Two parameters met the criteria of KFDA for bioequivalence, indicating that Bucilin^TM tablet is bioequivalent to Rimatil^TM tablet.

중심 정맥 도관 348례의 임상적 고찰

조남수,김용배,임경준 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

To evaluate clinical trends, central venous catheterization of 348 in total performed at the Chosun University Hospital from January 1993 through December 1994 were analyzed clinically & statistically according to sex, age, department, used veins and complications. The results are as follows : 1) Considering sexual distribution, male patients were 61.2% and female patients were 38.8%. 2) According to age categories, fourth decade of life was most frequent. 3) According to departmental distribution, general surgery was 38.5%, chest surgery was 21.0% and orthopedic surgery was 12.9%. 4) Subclavian vein used most frequently (74.1%), and femoral vein accounted for 14.9%, extermal jugular vein 7.5% internal jugular vein 3.4%. 5) Complications associated with subclavian vein catheterization using infraclavicular approach occupied 15.1%(39 cases) of total subclavian vein catheterization(258 cases), and among these complications pneumothorax occupied 43.6%, subcutaneous hematoma 28.2%, local infection 15.4%.

Molecular and Cytogenetic Analysis of Transgenic Plants of Rice(Oryza sativa L.) Produced by Agrobacterium-mediated Transformation

Cho,Joon-Hyeong,Kim,Yong-Wook 한국자원식물학회 2004 Plant Resources Vol.7 No.1

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ 수식 이미지 kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ 수식 이미지 proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

파킨슨씨병에서 Selegiline HCI (MAO-B^(�))의 효과 및 안전성

조기현,김명규,김태선,김병채,이승한,황인용,김준태,김종기 全南大醫大 2000 전남의대학술지 Vol.36 No.2