Metastasis associated genomic aberrations in stage II rectal cancer

Hong Zhao,Zhi-Zhou Shi,Rui Jiang,Dong-Bing Zhao,Hai-Tao Zhou,Jian-Wei Liang,Xin-Yu Bi,Jian-Jun Zhao,Zhi-Yu Li,Jian-Guo Zhou,Zhen Huang,Ye-Fan Zhang,Jian Wang,Xin Xu,Yan Cai,Ming-Rong Wang,Yu Zhang 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Genomic aberrations of rectal carcinoma, especially DNA copy number changes associated with metastasis were largely unclear. We aim to identify the metastasis associated biomarkers in stage II rectal cancer. Formalin-fixed, paraffin-embedded primary tumor tissues of stage II rectal carcinoma were analyzed by array-based comparative genomic hybridization, and genomic aberrations were identified by Genomic Workbench and SAM software. Copy number changes and mRNA expressions were validated by Real-time PCR in an independent rectal cancer samples. The results showed that the most frequent gains in stage II rectal cancer were at 1q21.2-q23.1, 3p21.31, 11q12.2-q23.3, 12q24.11-q24.31, 12q13.11-q14.1 and losses in 18q11.2-q23, 17q21.33-q22, 13q31.1-q31.3, 21q21.1-q21.3, 8p23.3-p23.1 and 4q22.1-q23. Twenty-two amplifications and five homozygous deletions were also identified. We further found that S100A1 (1q21.3-q23.1), MCM7 (7q22.1) and JUND (19p13.11) were amplified and overexpressed in stage II rectal cancer. Interestingly, the genomic aberrations affected 14 signaling pathways including VEGF signaling pathway and fatty acid metabolism. Most importantly, loss of 13q31.1-q34 and gain of 1q44 were associated with distant metastasis. Our results indicated that these metastasis associated genomic changes may be useful to reveal the pathogenesis of rectal cancer metastasis and identify candidate biomarkers.

Causal Relations between Exposome and Stroke: A Mendelian Randomization Study

Hong-Qi Li,Yi-Wei Feng,Yu-Xiang Yang,Xin-Yi Leng,Prof Can Zhang,Shi-Dong Chen,Kevin Kuo,Shu-Yi Huang,Xue-Qing Zhang,Yi Dong,Xiang Han,Xin Cheng,Mei Cui,Lan Tan,Qiang Dong,Jin-Tai Yu 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.2

Background and Purpose To explore the causal relationships of elements of the exposome with ischemic stroke and its subtypes at the omics level and to provide evidence for stroke prevention. Methods We conducted a Mendelian randomization study between exposure and any ischemic stroke (AIS) and its subtypes (large-artery atherosclerotic disease [LAD], cardioembolic stroke [CE], and small vessel disease [SVD]). The exposure dataset was the UK Biobank involving 361,194 subjects, and the outcome dataset was the MEGASTROKE consortium including 52,000 participants. Results We found that higher blood pressure (BP) (systolic BP: odds ratio [OR], 1.02; 95% confidence interval [CI], 1.01 to 1.04; diastolic BP: OR, 1.03; 95% CI, 1.01 to 1.05; pulse pressure: OR, 1.03; 95% CI, 1.00 to 1.06), atrial fibrillation (OR, 1.18; 95% CI, 1.13 to 1.25), and diabetes (OR, 1.13; 95% CI, 1.07 to 1.18) were significantly associated with ischemic stroke. Importantly, higher education (OR, 0.69; 95% CI, 0.60 to 0.79) decreased the risk of ischemic stroke. Higher systolic BP (OR, 1.06; 95% CI, 1.02 to 1.10), pulse pressure (OR, 1.08; 95% CI, 1.02 to 1.14), diabetes (OR, 1.28; 95% CI, 1.13 to 1.45), and coronary artery disease (OR, 1.58; 95% CI, 1.25 to 2.00) could cause LAD. Atrial fibrillation could cause CE (OR, 1.90; 95% CI, 1.71 to 2.11). For SVD, higher systolic BP (OR, 1.04; 95% CI, 1.00 to 1.07), diastolic BP (OR, 1.06; 95% CI, 1.01 to 1.12), and diabetes (OR, 1.22; 95% CI, 1.10 to 1.36) were causal factors. Conclusions The study revealed elements of the exposome causally linked to ischemic stroke and its subtypes, including conventional causal risk factors and novel protective factors such as higher education.

Isocoumarin Derivatives from the Sea Squirt-derived Fungus Penicillium stoloniferum QY2-10 and the Halotolerant Fungus Penicillium notatum B-52

Xin, Zhi-Hong,Tian, Li,Zhu, Tian-Jiao,Wang, Wen-Liang,Du, Lin,Fang, Yu-Chun,Gu, Qian-Qun,Zhu, Wei-Ming 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7

Two isocoumarin derivatives, stoloniferol A (1) and B (2), a known $5{\alpha}$, $8{\alpha}-epidioxy-23-methyl-(22E, 24R)-ergosta-6$, $22-dien-3{\beta}-ol$ (3), and a known dihydrocitrinone (4) were isolated from the ethyl acetate extract of the sea squirt-derived fungus, Penicillium stoloniferum QY2-10, and a halophilic fungus, Penicillium notatum B-52, respectively. Their structures were elucidated by spectroscopic methods and optical rotation. The stereochemistry of 2 was determined on the basis of different NOE experiments and chemical transformation. Compound 3 showed cytotoxicity against P388 cells, with an $IC_{50}$ value of 4.07 ${\mu}M$.

Purification and Characterization of Novel Bifunctional Xylanase, Xyn3, Isolated from Aspergillus niger A-25

( Hong Ge Chen ),( Xin Yan ),( Xin Yu Liu ),( Ming Dao Wang ),( Hui Min Huang ),( Xin Cheng Jia ),( Jin An Wang ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.7

Quercetin Attenuates Visceral Hypersensitivity and 5-Hydroxytryptamine Availability in Postinflammatory Irritable Bowel Syndrome Rats: Role of Enterochromaffin Cells in the Colon

Hong-Yan Qin,Kai-hong Zang,Xiao Zuo,Xin-An Wu,Zhaoxiang Bian 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.7

Intestinal enterochromaffin (EC) cell hyperplasia and increased 5-hydroxytryptamine (5-HT) availability play key roles in the pathogenesis of abdominal hypersensitivity of irritable bowel syndrome (IBS). This study aims to study the effect of quercetin on visceral pain and 5-HT availability in postinflammatory IBS (PI-IBS) rats. PI-IBS model rats were administered quercetin by gavage at doses of 5, 10, and 20 mg/kg for 14 days. Compared with normal rats, the visceral pain threshold of PI-IBS rats was markedly decreased and the abdominal motor response to colon distension was markedly increased. The EC cell count and 5-HT level, as well as tryptophan hydroxylase (TPH) protein, were all significantly elevated in PI-IBS rats, while the 5-HT reuptake transporter (serotonin transporter) was reduced. Genes that are responsible for enteroendocrine cell differentiation, that is, Ngn3 and pdx1, were significantly increased in the PI-IBS group. Quercetin treatment markedly elevated the pain threshold pressure and decreased the visceral motor response of PI-IBS animals; and EC cell density and 5-HT level, as well as TPH expression, in the PI-IBS group were all reduced by quercetin. Quercetin treatment also significantly reduced colonic expression of Ngn3 and pdx1 of PI-IBS. Findings from the present study indicated that the analgesic effect of quercetin on PI-IBS may result from reduction of 5-HT availability in the colon, and the regulatory role of quercetin in endocrine progenitors may contribute to reduced EC cells.

Ozone Synthesis and Regeneration of DPF using Dielectric Barrier Discharges

Xin-hong Chen,C. Purushothama,Jae-ou Chae,Yonghwan Kwak 한국자동차공학회 2006 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

Non-thermal plasma technology has many applications in various areas. One of the applications is regenerating diesel particulate filter (DPF). DPF is a widely applied device to control the particulate emission of diesel engines. However, clogging of the DPF occurs after hours of operation resulting in reduced engine efficiency. In order to solve this problem carbon soot collected inside the DPF should be cleaned out periodically. Conventional high-temperature removal processes easily leads to the breakage of DPF. Herein, low-temperature plasma formed in a dielectric-barrier discharge(DBD) reactor was used to form active oxidants such as ozone and monatomic oxygen. Two types of plasma reactors: surface DBD and volume DBD were studied. Experimentally, the effects of discharge power and frequency on the performance of DBD reactors were studied. It has found that design of surface DBD used in this study is much more efficient than volume DBD. Regeneration of the DPF was carried out with oxygen flow rate 3.5 L/min; with applied discharge power 19 W using volume DBD. It could remove 2.1g of carbon soot after a continuous regeneration for 2.5 hrs.

Risk Factors, Patterns, and Outcomes of Late Recurrence after Liver Resection for Patients with Hepatocellular Carcinoma (Analysis of a Multicenter Cohort over 15 Years)

( Xin-fei Xu ),( Jiong-jie Yu ),( Ju-dong Li ),( Hao Xing ),( Jun Han ),( Zhen-li Li ),( Han Wu ),( Han Zhang ),( Jian-hong Zhong ),( Yi- Sheng Huang ),( Ya-hao Zhou ),( Ting-hao Chen ),( Hong Wang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Late recurrence (> 2 years) after liver resection of hepatocellular carcinoma (HCC) is usually considered as multi-centric tumors or de novo cancer formation. We aimed to investigate risk factors, patterns and outcomes of late recurrence after HCC resection. Methods: From a multicenter database from 2001 to 2015, 734 patients who were alive and recurrence-free at 2 years after curative resection of initial HCC were enrolled into this retrospective study. Univariate and multivariate Cox-regression analysis were used to identify independent risk factors of late recurrence. Patterns, treatments and outcomes of late recurrence were investigated and analyzed. Results: During a median follow-up of 78.0 months after surgery, 303 patients (41.3%) developed late recurrence. Multivariate analysis revealed that cirrhosis, macroscopic vascular invasion, satellites, and tumor size > 5cm were independent risk factors of late recurrence. Among them, 273 (90.1%) were sole intrahepatic recurrence, 30 (9.9%) were concurrent intrahepatic and extrahepatic recurrence, and none of them was sole extrahepatic recurrence; 165 (54.4%) patients received curative treatments for recurrent HCC, including re-resection, transplantation and local ablation. Multivariate analysis showed regular postoperative surveillance and receiving curative treatments were two independent protective factors of prolonging survival for those patients with late recurrence. Conclusions: Late recurrence is correlated with cirrhosis and certain tumor-related characteristics of initial HCC. The patterns of late recurrence suggest that postoperative surveillance after 2 years of surgery could be adjusted and more targeted. Regular postoperative surveillance improves the probability to receive curative treatments again, yielding to better outcomes for patients with late recurrence.

Smartphone Addiction Among University Students’ During the Post-COVID-19 Era: The Role of Emotional Intelligence and Future Anxiety

Hong-xin Zhang 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.10

Objective Coronavirus disease-2019 (COVID-19) pandemic-related psychological symptoms can lead to smartphone addiction (SPA) risk and other behavioral disorders, thus impacting individuals’ mental health and well-being. The present study aims to propose a mediation model to investigate the association between emotional intelligence (EI) and SPA, and the mediating role of future anxiety (FA) during the post-COVID-19 era. Methods An online questionnaire including the Emotional Intelligence Scale, the Dark Future Scale, and the Smartphone Addiction Scale among university students from China, was conducted between September 14 and November 22, 2022. Finally, 1,154 valid questionnaires were collected. The reliability and confirmatory factor analysis results showed that all three scales had good reliability and validity. Results Structural Equation Model demonstrated that EI significantly and negatively influenced SPA (β=0.211, p<0.001), university students’ FA significantly and positively effected SPA (β=0.315, p<0.001), EI significantly predicted SPA in university students, and FA partially mediated the association between EI and SPA. The mediation effect of FA was 0.110, which accounted for 34.27% of the total effect. Bootstrap results furthermore tested the rigor of the mediating effect. Conclusion These findings broaden our understanding regarding the relationship between EI and SPA and the mediating role of FA, providing new sights for educators on how to reduce the risk of SPA when confronting the ongoing and possible future pandemics.

The Effect of Mn/Al Substitution on the Structural Stability and Magnetic Properties of Mn₃AlC

Xin-You Wang,Ping-Zhan Si,Hui-Dong Qian,Yang Yang,Hong-Liang Ge,Jihoon Park,Xin-Qing Wang,Chul-Jin Choi 한국자기학회 2019 Journal of Magnetics Vol.24 No.1

The structural stability and magnetic properties of Mn3+xAl1-xC antiperovskite with varied Mn/Al substitution were studied systematically. Single phase Mn3+xAl1-xC alloys with antiperovskite structure were obtained in samples with x = −1/4, 0, 1/4, 1/2. An additional Mn23C₆ phase was precipitated from Mn3+xAl1-xC antiperovskite for x = 3/4 while Mn23C₆ phase was formed as major phase for x = 1. The mutual substitution of Mn and Al atoms has substantial effect on the Curie temperature and the saturation magnetization of the Mn3+xAl1-xC alloys. In comparison with the as-cast alloys, the as-annealed Mn3+xAl1-xC alloys exhibit reduced Mn/Al substitutions after high temperature homogenization, which enhances the ordering of Mn and Al atoms in the lattices. The Curie temperature of the homogenized Mn3+xAl1-xC increases with increasing Mn substitution to Al. The Mn₃AlC alloy shows the highest saturation magnetization among all samples with varied Mn/Al ratios. Most samples show zero coercivity and zero remanent magnetization. The maximum value of the magnetic entropy changes of Mn2.75Al1.25C at 285 K is 2.26 J/㎏ K in fields up to 3 T.

중국 몽고족 문화상징에 대한 인식과 민족 정체성 확립을 위한 문화상징요소 연구

홍흠(Hong-Xin),곽암(Guo-Yan) 한국콘텐츠학회 2017 한국콘텐츠학회논문지 Vol.17 No.2

본 논문은 다양한 소수민족으로 구성된 중국의 가장 대표적 소수민족인 내몽고자치구의 몽고족을 중심으로 그들의 문화상징에 대한 인식과 민족 정체성 확립을 위한 문화상징요소를 설문조사를 통해 체계적으로 정립하여 향후 몽고족의 대외 홍보 및 디자인에 응용 가능한 학제적 기초를 마련하고자한다. 본 논문은 객관적 자료를 위해 몽고족 및 타민족 300명을 대상으로 설문조사 하였으며 그 결과 대부분 몽고족은 소수민족임에 자부심을 느끼고 있으며 소수민족을 대표하는 민족이라 인식하고 있다. 몽고족의 민족성은 사회성이 높고 장식하기 좋아하는 심미성이 있으며, 창의성, 신뢰성, 진취성이 높은 것으로 나타났다. 또한 문화상징의 디자인요소 선호도에서는 색상은 청색, 문양은 성길사한의 인물 문양, 재질은 마노를 선호하였으며 상징의미는 평화가 가장 높았다. 문화상징 활용은 축제 때 가장 많이 사용하는 것으로 나타났으며 의류에 가장 많이 적용되는 것으로 파악 되었다. 이런 문화상징을 내몽고의 정체성 확립 및 홍보를 위해 적극 사용해야 한다는 의견이 높았다. 몽고족 문화상징의 체계적 정립은 몽고족의 민족 정체성을 확립하는 중요한 일로, 민족적 의미와 문화적 사명 아래 민족문화자원과 잘 결합되어 소수민족문화의 발전에 도움이 될 뿐만 아니라 소수민족으로서 자부심을 고취시킬 것이다. This paper is about on the most representative ethnic of China Mongolian as the research object, through the questionnaire survey to establish the understanding of national cultural symbol and the system of national identity, and lay a theoretical foundation for the application of Mongolian communication and design in the future. In order to achieve the objectivity of the data, so a questionnaire survey was conducted on 300 populations of Mongolian and other nationalities. The result is that the majority of the Mongolians believe that as the Mongolian people have a sense of pride, and the Mongolian nationality is a representative of china. Mongolian is a kind of aesthetic, creative, reliable, aggressive and like the decoration of the nation. The cultural symbols for design elements are cyan, Gen Gi Khan graphics, agate, and peaceful meaning and so on. The cultural symbols are used for celebration, as well as clothing accessories. The symbol of culture has played a positive role in the establishment of Inner Mongolia identity and the propaganda of the nation. The construction of Mongolian cultural symbol system plays an important role in the establishment of Mongolian national identity. To combine the meaning of nation and the mission of culture with national cultural resources. It is not only to help the development of minority culture, but also to promote the sense of pride of ethnic minorities.