Reference Map of Soluble Protenis from Salmonella enterica Serovar Enteritidis by Two-Dimensional Electrophoresis

김곤섭,Eung-goo Lee,Yong-hwan Kim,정태성,Yong-seung Shin,Gee-wook Shin,Hui-guen Cha,Mi-rim Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis byTwo-Dimensional ElectrophoresisMi-rim Park, Eung-goo Lee, Yong-hwan Kim, Tae-sung Jung, Yong-seung Shin, Gee-wook Shin, Hui-guen Cha and Gon-sup Kim*Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, KoreaReceived May 17, 2003 / Accepted July 23, 2003J. Vet. Sci. (2003), 4(2), 143-149JOURNAL OFVeterinaryScience*Corresponding author: Gon-sup Kim Laboratory of Veterinary Biochemistry, Collage of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Jinju 660-701 Korea Tel: +82-55-751-5823, Fax: +82-55-751-5803 E-mail: gonskim@nongae.gsnu.ac.kr

항정신병약물 사용 중인 정신분열병 환자에서 올란자판으로의 교체 방법에 관한 연구(II) : Comparison of Safety 안전성 비교

안용민,권용실,권준수,민성호,박두병,양문정,소형석,송종호,신윤식,우행원,유범희,이홍석,정한용,한창환,김용식 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.5

연구목적: 이 다기관 공동임상연구는 사용 중인 항정신병약물을 ’직접 교체 방법’또는 ’시작-감량 교체 방법’중 한 가지 방법으로 올란자핀으로 교체한 후, 안정성 측면에서 두 교체 방법 간의 비교와 교체후의 변화를 관찰하기 위한 것이다. 방법: 국내 13개 병원의 입원 및 외래에 내원한 환자들 중 ICD-10 지단기준으로 정신분열병에 해당되며, 임상적으로 항정신병약물 교체가 필요한 환자를 대상으로 하였다. 두 가지 교체 방법 중 한 가지를 무작위로 피험자에 적용하였으며, ’직접 교체 방법’에 배정된 경우에는 사용중인 항정신병약물을 일시에 중단하고 10㎎의 올란자핀을 바로 투여하였고, ’시작-감량 교체 방법’에 배정된 경우는 10㎎의 올란자핀 투여하고 2주에 걸쳐서 기존 약물을 감량하여 중단하였다. 올란자핀 사용기간은 총 6주이며, 용량은 5∼20㎎ 범위로 제한하였다. 한정성 평가를 위해서 체중, 생명징후, 자발적인 이상반응 복, 실험실 검사 그리고 Simpson-Angus Scale(SAS), Barnes akathisia rating scale(BARS), Abnormal involuntary movement scale(AIMS). Liverpool University neuroleptic side effect rating scale(LUNSERS)등을 이용하였다. 결과: 총 103명의 정신분열병 환자를 대상으로 하였다. 사용한 올란자핀의 용량, 벤조디아제핀의 병용률, 탈락률과 탈락 사유, 자발적인 이상반응 보고, 생명징후, 실험실 검사 그리고 대부분의 부작용 척도 상에서 임상적으로 의미 있는 차이를 두 교체 방법간에 발견하지 못하였다. 다만 AIMS의 감소는 ’직접 교체 방법’군에서 보다 적었고, 항콜린제의 병용률은 ’시작-감량 교체 방법’군에서 보다 많았다. 기저 상태에서 전체 피험자의 SAS와 BARS 점수는 각각 3.5점과 1.8점이었으며 70% 이상의 피험자가 고프로락틴 혈증을 보였다. 올란자핀으로 교체한 후, SAS, BARS, AIMS 점수의 유의한 감소가 있었으며 고프로락틴 혈증을 보인 피험자 분율도 약 30%이하로 감소하였다. 그러나 교체 방법과 상관없이 올란자핀 교체 후 유의한 체중 증가가 있었다. 결론: 이 연구를 통해 교체 방법에 관계없이 비교적 안전하고 용이하게 올란자핀으로 교체 할 수 있음을 알 수 있었다. 그리고 기존 항정신병약물을 올란자핀으로 교체함으로써 일부 부작용들을 줄일 수 있음을 간접적으로 관찰할 수 있었다. 하지만 이 연구는 여러 제한점과 문제점을 지니고 있기 때문에 보다 체계적인 연구를 통해 검정이 필요하리라 생각된다. Objectives: This multicenter clinical trial involving 13 hospital sites compared the safely of switching to olanzapine between ’direct switching method’ and ’start-tapering switching method’. Method: This study included both inpatients and outpatients who fulfilled the criteria for schizophrenia as defined in the ICD-10, and were in need to be appropriate for switching antipsychotics. Subjects were randomly assigned to one of the two switching methods. For ’direct switching method’group, previous antipsychotics were abruptly discontinued and 10㎎ of olanzapine was administered, and previous antipsychotics was gradually tapered for 2 weeks. Olanzapine was used for 6 weeks and the dose was adjusted within the range of 5-20㎎. The safety of switching to olanzapine was measured with vital sings including body weight, adverse events reported spontaneously, laboratory tests, and various scales such as Simpson-Angus Scale(SAS), Barnes Akathisia Rating Scale(BARS). Abnormal Involuntary Movement Scale(AIMS), and Liverpool University Neuroleptic Side Effect Rating Scale(LUNSERS). Results: 103 patients were switched to olanzapine in this study. The comparison between two switching methods did not show any significant difference in the dosage of olanzapine used, the concomitant use of benzodiazepine, the rate and reasons of drop-out, the adverse events, vital signs, laboratory tests, and most scales for measuring side-effects. However, the decrease in AIMS scores was significantly lower in ’direct switching method’ group, and the concomitant use of anticholinergics was comparatively greater in ’start-tapering switching method’ group. At baseline, SAS and BARS scores were 3.5 and 1.8 points respectively, and more than 70% of the subjects showed hyperprolactinemia. After switching to olanzapine, SAS, BARS, and AIMS scores were significantly decreased and the proportion of the patients with hyperprolactinemia was also decreased to less than 30%. However significant weight gain after the treatment of olanzapine was observed regardless of switching method. Conclusion: This study may suggest that switching to olanzapine can be done with relatively high safety regardless of switching methods and olanzapine can significantly decrease some side-effects induced by other antipsychotics.

자기-관찰학습이 피루에트 앙 디올 턴 학습에 미치는 영향

신석환(Shin, Seak-Hwan),권택용(Kwon, Taeg-Yong) 한국사회체육학회 2015 한국사회체육학회지 Vol.0 No.60

This study is a form of observation learning is intended to examine the applicability of the observation learning program for the promotion of dance skills learning in real lesson in the dance scene by analyzing the impact of Pirouette en dehors turn learning. This study is no dance experience with a total of 36 groups o f between 17 and 24 years old, men and women and target the expert-modeling group, self-modeling group , self-observation groups, were divided into a control group with nine apiece randomly assigned. After subjects are to conduct a preliminary examination of each about after a pirouette en dehors turn each group participated in training for seven days of practice for 20 minutes every day, depending on how. After the end of practice to identify changes in the entire performance during the time trial performance by filming a scene. Immediately posttest examination was performed one day after the end of experiment. The analysis of operations carried out in the retention test score of self-observation group performs scores were better than the other groups, self-modeling group, expert-modeling group and the control group was found to improve skills in order to perform this operation.

혈중 감마지티피 상승의 임상적 의의

신주연,김용규,박신구,이지나,김환철,임종한,홍윤철 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.1

목적: 혈중 감마지티피는 주로 알코올 섭취와 간세포 손상의 지표로서 이용되어져 왔으나 최근에는 혈중 감마지티피가 체내의 산소성 스트레스를 민감하게 반영하는 지표이며, 혈중 감미지티피를 이용하여 만성질환의 발생을 조기에 예측할 수 있다는 주장들이 있다. 이에 본 연구에서는 일반 인구 집단을 대상으로 한 건강검진 자료를 분석하여 혈중 감마지티피의 상승에 영향을 미치는 요인들을 분석하고 혈중 감마지티피와 산소성 스트레스와의 관련성 및 임상적인 이용 가능성을 알아보고자 한다. 방법: 2001년부터 2003년까지 한 대학병원 산업의학과에서 건강검진을 실시한 15,546명을 대상으로 하였고 신체계측, 혈액검사 그리고 설문지의 정보를 분석하였다. 총 15,546명 중 남성은 9,660명 이었고 여성은 5,886명 이었다. 연령, 성별, 체질량지수, 흡연, 음주, 운동량 비타민 복용 여부에 따른 혈중 감마지티피의 평균을 분석하였고, 성별에 따른 다변량 분석을 실시하였다. 다변량 분석에는 연령, 체질량지수, 음주빈도, 흡연량, 혈색소, 이완기혈압, 공복시혈당, 총콜레스테롤, ALT를 포함하였다. 결과: 연구 대상자들의 혈중 감마지티피의 평균은 남성에서 평균(±2SD)이 47.51(±111.62)이었고, 여성에서는 19.09(±42.02) 으로서, 대체적으로 정상 범위에 있다고 보여 지나 개인차가 상당히 크다는 사실을 알 수 있었다. 다변량 분석의 결과에서는 혈중 감마지티피의 상승에 영향을 미치는 요인이 성별에 따라 다소 달랐으나 남녀 모두에게 연령, 음주빈도, 흡연량, 이완기혈압, 공복시혈당, 총콜레스테롤, ALT가 유의한 영향이 있음을 확인할 수 있었다. 그리고 다변량 로지스틱회귀분석의 결과에서 '임상적 비정상'을 예측함에 있어 혈중 감마지티피가 진단적 유용성이 있음을 확인하였다. 결론: 긴존에 일반적으로 알려져 있는 것처럼 혈중 감마지티피를 단순히 알코올 섭취와 간세포 손상의 지표로서만 볼 수 없으며, 성별, 연령, 흡연, 고지혈증, 당뇨, 고혈압 등 많은 다른 요인들에 의해서 영향을 받으며, 혈중 감마지티피를 산소성 스트레스를 반영하는 생물학적 지표로 해석할 수도 있다. 따라서 혈중 감마지티피의 상승을 임상적으로 해석할 때는 (비록 정상인 범위에 있다하더라도), 알코올 섭취나 간세포 손상 뿐 아니라 다른 여러 요인들을 함께 고려해야 한다. 더욱이 혈중 감미지티피의 상승을 만성질환 즉, 고혈압, 당뇨, 고지혈증 등의 발생을 조기에 발견할 수 있는 지표로서 임상영역이나 건강검진시 유용하게 활용할 수 있을 것이다. Objectives: Incresed gamma-glutamyltransferase (GGT) concentration has mainly been used as a biologic marker of alcohol drinking and liver diseases. However, some recent reports have suggested that serum GGT concentration may be an early biomarker of oxidative stress and associated with chronic diseases like hypertension, DM, hyperlipidemia, CHD, stroke etc. In this study, we analysed the factors associated with increased serum GGT level and evaluated the clinical application of serum GGT in predicting the risk of chronic diseases. Methods: The data were collected from 15,546 periodic health examinations, 9,660 males and 5,886 females, done at one university hospital from 2001 to 2003. We analysed self-questionnaire, physical examination and laboratory data. Statistical analyses(t-test, ANOVA, Pearson's correlation analysis, multiple regression analysis and logistic regression analysis) were done by SPSS for windows 10.0. Results: Mean serum GGT levels were quite different according to age, sex, BMI, smoking and alcohol drinking (all P-value <0.001), but were not different by regular vitamin intake or not (P-value = 0.117). There was significant correlation between serum GGT level and the variables (of age, BMI, blood pressure, Hb, fasting blood sugar, total cholesterol, AST and ALT) (all P-value <0.001). In sex-specific multiple regression analysis, increased serum GGT level was significantly associated with age, alcohol drinking, smoking, blood pressure, fasting blood sugar and ALT in males (all P-value <0.001), and with age, alcohol drinking, smoking, Hb, blood pressure, total cholesterol and ALT in females (all P-value <0.05). In sex-specific logistic regression analysis, increased serum GGT level was significantly associated with risk of 'clinical abnormalities'. This risk increased about 13-fold in males and 4.6-fold in females for serum GGT level over the third quatile, compared with under the first quartile. Conclusions: Serum GGT level is increased not only by alcohol drinking or liver diseases, but also by many other factors associated with chronic diseases and behavioral factors. Thus many factors should be considered in evaluating an increase in GGT level, even when within the upper normal range. Serum GGT level may be a predictor for the early development of chronic diseases.

구리의 전기석출시 젤라틴이 물성에 미치는 영향

신석재,강현춘,안형환,강안수,김용렬,한성만,이한섭 明知大學校 産業技術硏究所 1995 産業技術硏究所論文集 Vol.14 No.-

In the electrodeposition of copper foil for preparing printed circuit boards, a little amount of gelatin as a addition agent has been used for the better characteristics of a coat of electroplating. Gelatin modifies the physical properties such as mechanical strength, matte-side roughness, ultimate tensile strength(U.T.S), and elongation of copper foil, furthermore gelatin have influence on the structure of electrodeposits and the cathode polarization. The electrolyte used was of a composition; CuSO₄·5H₂O 300g/ℓ, H₂SO₄ 100g/ℓ, NaCl 10 ppm as a brightner, and was gelatin 0-7 ppm as a addition agent. Current density was 50 A/dm², and the temperature of electrolytic bath was 50 to 52 ℃. Experiments showed that the optimum gelatin concentration was 5 ppm with the consideration of the numbers of electrodeposited nuclei. And the nuclei numbers was sharply increased under 5 ppm, but was almostly constant above 5ppm. Average matte-side roughness by Surfcorder was gradually increased with the increasing gelatine concentration up to 2 ppm, and maximum roughness was observed at 2ppm. The roughness showed gradual decrease within the concentration range of 2 to 4 ppm, and was almost constant above 4ppm . Within the gelatine concentration range of 0 to 7 ppm, as the concentration increased, UTS was increased and elongation was decreased logarithmically. Also, in comparison between UTS and elongation, gelatin was identified as an addition agent acting inversely.

트랜지스터를 使用한 高安定 直流定電壓回路에 關한 硏究

辛烘圭,金煥溶 圓光大學校 1983 論文集 Vol.17 No.2

A low stability is in conventional current control type of regulator circuit using operational amplifier, i.e, when the base current of controlling main transistor varies by the ac input variation and the reference voltage varies by the load current variation, dc output voltage varies considerably. In order to solve this problem, an improved regulator circuit using transistor is proposed in this paper. As a result of experiment, its stability is improved remarkably notwithstanding the wide variation of the input voltage and load current.

忠南地域 觀光農業의 開發方案 Ⅱ : 觀光農業 第一段階의 開發模型 First Stage Development Model

愼鏞仁,申東完,林栽煥,金再洪 忠南大學校 地域開發硏究所 1990 地域開發論叢 Vol.2 No.-

한국재래흑염소에서 발정 및 과배란 유도와 외래유전자 주입에 적합한 1세포기 수정란의 채취

신상태,이두환,김명철,이운규,이철상,한용만,이경광 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

Three different treatments for induction of estrus in Korean native black goats were compared: follicle stimulating hormone(FSH, FSH-p^TM), FSH combined with MAP(intravaginal impregnated sponges, Veramix^ⓡ, containing 60 ㎎ medroxy progesterone acetate for 14 days), and FSH combined with progesterone(Ovaron^ⓡ, 10 ㎎ IM for 10 days) and PGF_2α(Lutalyse^ⓡ, 3 ㎎ IM at first FSH injection). FSH for inducing estrus and superovulation was given a total 20 ㎎ intramuscularilly in decreasing dosage injections twice daily over 4 days. The MAPs were withdrawn at the 3rd day of FSH injection. Estrus observations were conducted every 6 hours from last FSH injection for 24 hours by placing the does with fertile male goats. Estrus and superovulation were more successfully induced with treatment of MAP + FSH than other treatments(FSH only, or progesterone + PGF_2α + FSH) (estrus induction; 100 vs 42.8 and 71.4%, ovulation points; 11.4 vs 5.4 and 4.4, respectively). The effect of gonadotropin releasing hormone(GnRH) on the ovulation rate was also examined. However, no difference was observed for inducing ovulation with treatment or dosage(100 ㎍ 200 ㎍) of GnRH. Low midline laparotomies were performed, and then ovarian responses (ovulations and follicular development) were examined by exteriorization of the reproductive tracts. Ova were recovered from oviducts by retrograde flushing 60-146 hours after MAP removal, and were classified the developmental stages. Overall 66.1% (236/357) of recovery rate was obtained from 30 superovulated does. The optimal recovery time of microinjectable 1-cell zygotes was approximately 72-76 hours after MAP removal.

노인종합복지관의 이용특성에 따른 이용권 형성에 관한 건축 계획적 연구

신대진,정준수,박용환 대한건축학회 2004 대한건축학회논문집 Vol.20 No.8

The purpose of this study is to analyze the choice of the General Welfare Facilities for the Aged at the view of the distribution of the users and to help to plan the welfare facilities. The number of users' residence is analyzed as compared with factors of transportations and living conditions to find out the characteristics of facility and the distance of using. The 2903 registers in one year are reserved for analyzed and 233 persons also are surveyed in three facilities. The results of this study are as follows; The distribution of using is following to political district and biased against to others. The average catchment area of the facilities is 800m on feet and has diversity according to the transportations and living condition of users.

AWRR 셀 스케쥴링 알고리즘

신승수,신동화,지홍일,최승권,조용환 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.1

The Binary Scheduling Wheel(BSW) algorithm was proposed to carry on manage buffer efficiently at implementing of WRR scheduler. But, BSW algorithm cause serous performance degradation of cell delay and maximum queue length because it allocates more weight than the weight allocated actually in VC and could not serve cell if the VC queue is empty. In this paper, we propose new BSW of the old BSW structure. Also, we develop a cell scheduling algorithm which is suitable for the new BSW structure. The proposed BSW structure and the developed algorithm are capable of maintaining the allocated weight of each VC correctly, and decrease average cell delay and average buffer length by serving other VC cell when the selected VC queue is empty, and increase cell service ratio as a whole. The evaluation results show that the proposed structure can implement WRR algorithm more efficiently than the old BSW.