재조합유전자의 미세주입이 소 난포란의 체외발생에 미치는 영향

이철상,한용만,박정선,강용국,김선정,유대열,이경광 한국동물생명공학회(구 한국동물번식학회) 1993 Reproductive & developmental biology Vol.17 No.3

각종 폐장질환 환자의 혈청 Lactic Dihydrogenose 활성도에 관하여

이철상,박희명,문세광,류호렬 대한결핵 및 호흡기학회 1962 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.15 No.-

Goat β-casein Promoter Directs hGH Expression at a High Level and in a Mammary-Specific Manner in Transgenic Mice

이철상 한국유전학회 2006 Genes & Genomics Vol.28 No.2

In this study, goat β-casein/hGH hybrid gene-harboring transgenic mice were generated and transgene expression was assessed in the milk, blood, and various organs of the transgenic females. All of the founder females (16 lines) were fertile, and all secreted hGH into their milk. Milk hGH concentration was found to be consistently high; it was ranging from 0.1 to 13.2 mg/ml and higher than 1 mg/ml in most of the transgenic mice (10/16 lines). The blood hGH levels in the higher-expressing mice (> 5 mg/ml in milk) was considerably elevated at lactation (12.6 ± 11.3 g/ml), but was greatly reduced to a concentration of 9.9 ± 9.1 ng/ml during the non-lactation stage, and was even lower, 2.5 ± 1.7 ng/ml, in the males. Despite the presence of hGH in the blood of lactating transgenic mice, Northern blot analysis revealed that the expression of hGH was highly specific to the mammary glands. These results indicated that a strict temporal and spatial regulation of goat β-casein/hGH gene expression, which resulted in very low blood hGH levels during non-lactation phases, could induce a consistently high level of hGH expression in the milk of transgenic mice with no apparent systemic effects on reproduction, most notably infertility.

韓·日 經濟協力의 現況과 問題點

이철상 西江大學校 經商大學 1976 經商評論 Vol.- No.3

Integration and Expression of Goat β-Casein/hGH Hybrid Gene in a Transgenic Goat

이철상,Doo Soo Lee,Nanzhu Fang,Keon Bong Oh,Sang-Tae Shin,Kyung-Kwang Lee 사단법인 한국동물생명공학회 2006 Reproductive & developmental biology Vol.30 No.4

In order to generate transgenic goats expressing human growth hormone (hGH) in their mammary glands, goat β-casein/hGH hybrid gene was introduced into goat zygotes by pronuclear microinjection. DNA-injected embryos were transferred to the oviduct of recipients at 2-cell stage or to the uterus at morula/blastocyst stage after cultivation in glutathione-supplemented mSOF medium in vitro. Pregnancy and survival rate were not significantly different between 2-cell embryos and morula/blastocysts transferred to oviduct and uterus, respectively. One transgenic female goat was generated from 153 embryos survived from DNA injection. Southern blot analysis revealed that the transgenic goat harbored single-copy transgene with a partial deletion in its sequences. Despite of the partial sequence deletion, the transgene was successfully expressed hGH at the level of 72.1±15.1 μg/ml in milk throughout lactation period, suggesting that the sequence deletion had occurred in non-essential part of the transgene for the transgene expression. Unfortunately, however, the transgene was not transmitted to her offspring during three successive breeding seasons. These results demonstrated that goat β-casein/hGH gene was integrated into the transgenic goat genome in a mosaic fashion with a partial sequence deletion, which could result in a low level expression of hGH and a failure of transgene transmission.

胃冷凍에 關한 實驗的硏究 : 胃液分泌 胃粘膜의 組織學的 所見 心電圖 및 肝機能에 對하여 On gastric secretion, histology of the gastric mucosa, electrocardiogram and liver function

李澈相 現代醫學社 1968 現代醫學 Vol.9 No.6

Since Wangensteen and his associates introduced the technique of gastric freezing as an ulis, Histoge effective method for the treatment of peptic ulcer disease in 1962, many experimental and 13, 1952. clinical studies have been performed and conflicting results began to appear concerning the s, ain. North effectiveness of the technique. First of all, some observers claimed that the depression of gastric secretion was not always as impressive as Wangensteen had reported and they attriral Lesions, buted this to the possibility of patchiness of the freezing effect on the gastric mucosa which 'd. &Oral Path.. may also cause the known serious complications such as gastric hemorrhage, ulecerations 59 4 th Ed. 1954, or perforations. To improve the efficacy of the gastric freezing and to eliminate the compli-,ulis" Oral Surd cations, many modified techniques were attempted and some reported that the rapid rewarming following freezing showed more effective reduction of gastric acid secretion and 1953. decrease of the complications. In view of these facts, the present study was designed to determine whether the more. ongenital Ep effective gastric freezing could be accomplished by repeated gastric freezings which may a'cover the patchiness of a single freezing and to compare the effectiveness of repeated freez Dent ings with and without rewarming. All experimental dogs were given gastrotomies with stainless tubes at the most dependent portion of their stomaches for the gastric analysis which was performed every othe after each freeze, and then every week for five weeks. For the preliminary test, 12 dogs were used to determine the minimal histamine do optimal gastric secretory responses by injecting histamine diphosphate in graded doses' 4.02 mg/kg to 0.12mg/kg. The minimal histamine dose for the optimal gastric sec response in dogs was found to be 0.10 mg/kg. and at this_ dose the means of the voj` of gastric secretion, acidity and acid output were 80ml/hr., 100.0 mEq/L and 7.2 m respectively. For the freezing experiment, 18 dogs were utilized and they were.divided into the foA: wing three groups: Group I (control group): In this group of four dogs, gastric analysis was done wee without freezing or rewarming. Group II: In this group of four dogs, a single freezing was accomplished with rapid rew arming in half of the,number (Group II-a) and without rewarming in the other hay (Group II-b). Group III: In this group of 10 dogs, three successive gastric freezing with rewarming, e one week intervals, were done in 3 of the dogs (Group III-a), and without rewarming, the same one week intervals, in the other seven (Group III-b). Gastric secretory and, histologic alterations as well as liver function and electrocardiograph " changes after or during the gastric freezing were observed and the results were summariz as follows: 1) There were a considerable variation and occasional rebound phenomena in the volume, acidity and hourly acid output and no dramatic reduction in all three modalities followin both a single and three successive gastric freezings. However, three successive freezings.' produced more consistant and greater reduction in the volume and hourly acid output than a single freezing did. Rapid rewarming following freezing appeared to reduce the effects of freezing. There was no appreciable alteration of pH of gastric juice after freezing. 2) There were no significant histological and macroscopical changes in the stomach 6' . weeks after the last gastric freezing. 3) There was no appreciable alteration in total protein, albumin, globulin,thymol turbidity test and alkaline phosphatase activity after gastric freezing, but BSP dye retention examined at the end of gastric freezing was eight times higher than control. 4) Electrocardiogram recorded during gastric freezing showed gradual bradycardia in 5550, prolongation of QRS and Q-T intervals, depression of ST segment and lowering or inversion 4 of T waves in 100%, and occasional ventricular extrasystoles.

폐결핵환자의 동맥혈의 호흡성 Gas의 변동에 관한 연구 - 안정시의 관찰(1편) -

이철상,박희명 대한결핵 및 호흡기학회 1962 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.15 No.-

多發性 胃 Polyp의 1例

李澈相,鄭俊謨,趙世煥,李泰淑,孫泰重,洪善熹,鄭克守 慶北大學校 醫科大學 1971 慶北醫大雜誌 Vol.12 No.1

육종 ; 우유의 모유화를 위한 형질전환 동물 개발에 관한 연구 3. 사람 락토페린 유전자 ( CChcLf ) 가 도입된 소 난포란의 체외 발달

이철상,박윤호,박정선,조용연,한용만,유대열,이경광,신상태,김상근 한국동물자원과학회 ( 구 한국축산학회 ) 1993 한국축산학회 심포지움자료집 Vol.- No.-

배아줄기세포 유래 신경계세포에서의 세포사멸 연구와 그 응용

이철상 한국발생생물학회 2008 발생과 생식 Vol.12 No.1

배아줄기세포는 다양한 분화유도 방법을 통해 신경계세포로 분화시킬 수 있을 뿐만 아니라, 보다 더 엄격한 선발조건을 적용함으로써 특정 종류의 신경세포만을 확보할 수도 있게 되었다. 세포사멸연구를 포함한 신경생리학적 연구의 대상으로써 중요한 요건은 이렇게 확보한 배아줄기세포 유래의 신경계세포들이 정상적인 신경생리학적 특성을 갖고 있어야 하며, 동시에 그런 신경생리학적 특성이 체외에서 일정기간 동안이상 자연적인 세포사멸없이 유지되어야 한다는 것이다. 생쥐배아줄기세포를 retinoic acid로 처리한 후, astrocytes monolayer 위에서 신경계세포로 분화시키면 장기간 생존이 가능한 다수의 신경계세포를 손쉽게 확보할 수 있을 뿐만 아니라, 면역세포화학적 방법을 통해 신경세포의 생사를 개별세포 수준에서 추적할 수 있다. 배아줄기세포 유래의 신경계세포는 glutamate agonist들에 대해 수용체 특이적 흥분성 신경독성반응을 보이며, 이 반응은 신경계세포로의 분화가 진행될수록 더욱 뚜렷해지는 양상을 보인다. 신경계세포의 발생분화, 생존에 관여하는 Neurotrophin, GDNF계열의 신경계 작용 성장인자들의 수용체가 배아줄기세포 유래의 신경계세포에서 발현되고 있으며, 이들에 의해 신경계세포로의 분화과정에서 세포의 생존능 및 신경독성처리에 대한 세포사멸반응이 조절될 수도 있다. 따라서 배아줄기세포 유래의 신경계세포는 신경세포의 생존과 사멸, 그리고 세포손상으로부터의 보호와 같은 신경약리학적 연구를 위한 중요한 특성을 나타내고 있기에 관련 연구를 위한 새로운 연구시스템이 될 수 있는 것이다. 특히 일반적인 신경세포는 유전적 변형이 어려워 다양한 신경약리학적 연구에 많은 제약을 받아 왔으나, 이제 유전자변형 배아줄기세포로부터 얻은 신경세포를 활용하여 연구할 수 있게 됨으로써, 보다 복합적인 신경약리학적 기초연구도 가능하게 되었다. 특히 최근 인간배아줄기세포 유래 신경계세포도 유사한 신경독성 반응을 보이고 있음이 확인됨으로써(Schrattenholz & Klemm, 2007), 이제 배아줄기세포는 신경약리학적 기초 연구만이 아닌, 나아가 대량의 약물 스크리닝과 같은 제약산업에도 활용될 수 있는 가능성을 제시하고 있다.