간세포암과 주위 조직에서의 Transforming Growth Factor-beta 1과 그 수용체의 발현

최규완,백승운,최문석,김소정,이준혁,고광철,이풍렬,이종철,박상종,안병훈,김재준 대한소화기학회 2001 대한소화기학회지 Vol.37 No.4

Background/Aims: Transforming growth factor-beta 1 (TGF-β1) is a potent inhibitor of hepatocyte proliferation and its receptor is known to be involved in the carcinogenesis. This study was designed to compare the expression levels of the TGF-β1 and its receptor in neoplastic cells and its surrounding tissue and to assess their role in hepatic carcinogenesis. Methods: We measured the levels of TGF-β1 mRNA and TGF-β1 receptor II mRNA by semiquantitive RT-PCR for the tumor and its surrounding tissue of 30 patients with hepatocellular carcinoma (HCC). We analyzed the relationship between the expression of TGF-β1 mRNA, TGF-β1 receptor II mRNA and clinicopathological characteristics of HCC. Results: The expression of TGF-β1 mRNA showed no difference between HCC and its surrounding tissue, but the expression of TGF-β1 receptor II mRNA was lower in HCC than in its surrounding tissue (2.47±1.08 vs 3.53±1.50, p=0.00). There was no difference in the expression level of TGF-β1 mRNA and TGF-β1 receptor II mRNAo the clinicopathological characteristics of HCC. However, the ratio of TGF-β1 receptor II mRNA in HCC to that in surrounding tissue was more decreased in HCC with portal vein invasion (0.49±0.22 vs 0.77±0.21, p=0.01). Conclusions: Our data suggest that the loss of TGF-β1 receptor II may play an important role in carcinogenesis and tumor progression of HCC.

췌장암에서 Cyclooxygenase - 2 발현의 역할

김은경,이용욱,이규택,최규완,박동일,백승운,이준행,이종균,고광철,이풍렬,이종철,김재준,김완선,공구 대한소화기학회 2001 대한소화기학회지 Vol.38 No.3

Background/Aims: Accumulating evidence suggests that the use of NSAID may reduce the incidence of colorectal cancer. The likely mechanisms of these effects by NSAID is cyclooxygenase-2 (COX-2)-related inhibition of tumor proliferation and induction of apoptosis. The aim of our study was to examine possible roles and clinical significance of COX-2 expression in pancreatic cancer. Methods: Seventy-two pancreatic adenocarcinoma tissue specimens were obtained from surgical resection. After the immunohistochemical staining of the specimens, we examined proliferation activity (assayed by Ki-67 expression), apoptosis (by TUNEL stain), and microvessel density (by CD34 expression). We also investigated the relationship between the immunohistochemical expression of COX-2 and various clinicopathological characteristics. Results: The COX-2 positive rate in pancreatic epithelial cells was 41.7%. Proliferation index (PI) was significant higher in COX-2 positive specimens comparing to negative specimen (p=0.015) and the increase in intensity of COX-2 expression correlated with increasing PI (p=0.036). Apoptotic index (AI) was significantly higher in positivee COX-2 expression than in negative expression (p=0.044), but there was no significant difference in AI/PI between the COX-2 positive and negative specimens (p=0.44). The expression of COX-2 protein did not correlate with microvessel density, sex, age, differentiation, tumor size, stage, metastasis or patients survival. Conclusions: The expression of COX-2 enzyme in pancreatic cancer contributes to tumor proliferation, but is not related to apoptosis, angiogenesis or clinical characteristics. Further study is needed to examine the clinical usefulness of NSAID and COX-2 selective inhibitors.

역류성 식도염 환자에서 Helicobacter pylori 치료 1 년 후 증상과 내시경적 변화

김영호,손희정,최규완,박동일,백승운,이준행,고광철,이풍렬,이종철,임윤정,김재준 대한소화기내시경학회 2001 Clinical Endoscopy Vol.24 No.1

활동성 크론병에서 초기 스테로이드 치료 반응에 따른 임상적, 생화학적, 병리학적 인자들에 관한 연구

김영호,장재권,김지은,최규완,박동일,도재혁,백승운,고광철,이풍렬,이종철,현재근,안병훈,김재준,류민규 대한소화기내시경학회 2001 Clinical Endoscopy Vol.22 No.6

Background/Aims: The predictory factors of the response to initial steroid therapy in active Crohn's disease has been controversial in numerous literature reviews. We evaluated any predictory faetor of the response to initial steroid therapy in active Crohn`s disease patients, Methods: The medical records of 32 patients with active Crohn`s disease who clinically responded to oral steroid therapy were retrospectively reviewed. The steroid responsive group was defined as the one showing maintenance of response for more than one month from steroid withdrawal and the steroid dependent group as the one showing relapse or exacerbation during steroid tapering or within 30 days from steroid withdrawal. The clinical, biochemical, and pathologic factors were evaluated. Results: There were 22 male and 10 female patients. The mean age was 28.9 years. The number of steroid responsive and dependent group was 22 (68.8%) and 10 (31.2%), respectively. There were no significant differences between these two groups in age, sex, time to diagnosis, perianal lesion, extent of disease, extraintestinal manifestations, presence of granuloma, presenting features, hemoglobin, ESR, and CRP, except serum albumin level. Conclusions: Serum albumin level was significantly lower in steroid dependent group than steroid responsive group, reflecting severe inflammation in steroid dependent group.

허혈성 대장염의 임상 양상과 예후 인자

이미숙,손희정,강태욱,최규완,박동일,백승운,이준행,고광철,이종철,임윤정,이풍렬,김갑철,김재준 대한소화기내시경학회 2001 Clinical Endoscopy Vol.22 No.2

Background/Aims: Colonic ischemia is recognized as the most common vascular disorder of the intestines in the elderly. The severity of the disease may vary considerably with the prevalent benign transient forms, being amenable to conservative treatments and the severe gangrenous forms, requiring surgery. Correct diagnosis is usually made after the ischemic episode is over and colonic ischemia is frequently misdiagnosed. The aim of this study is to identify the clinical patterns and prognostic factors of ischemic colitis. Methods: We found 60 patients with nonoccusive ischemia of the large intestine, We analyzed age, sex, symptoms, signs, associated disease, location, endoscopy, hematologic, histologic, radiologic finding and treatment, retrospectively. Results: The mean age was 60.2±6.3 years. The most common symtoms were abdominal pain or bloody diarrhea. The most common sign was diffuse abdominal tenderness. The most common hematologic findings were anemia and leukocytosis. Associated medical illnesses wer diabetes, rhematoid arthritis and cerebrovascular accident, et al. Both computed tomography (CT) and endoscopy were useful diagnostic tools. The most common involved site was sigmoid colon. 46 patients were treated conservatively and 14 patients were managed surgically. Conclusions: Male gender, those with rebound tenderness, severe anemia, right or total colon involvement as well as rheumatoid arthritis had a poor prognosis. Both CT and endoscopy were useful in predicting prognosis.

성인에서 반복적인 위장관출혈로 발현한 공장 거대 중복성 낭종

김영호,이경수,이상수,문원,강태욱,최규완,박동일,도재혁,백승운,이풍렬,이종철,김재준,류민규 대한내과학회 2001 대한내과학회지 Vol.61 No.3

Duplication of the alimentary tract is an unusual congenital anomaly which may occur at any level, from the oral cavity to the rectum. Jejunal duplication cyst is quite rare and most of them are presented in infancy with the symptoms of abdominal mass, obstruction, hemorrhage and perforation. But some cases has not been diagnosed till adult if duplication cyst was too small enough to cause symptoms. We report a case of a 28-year-old woman who presented with recurrent gastrointestinal hemorrhage as a result of an ulceration without ectopic mucosa in jejunal duplication cyst and was successfully treated with surgery.(Korean J Med 61:264-269, 2001)

위장관 간질종양의 임상 및 병리학적 고찰

김영호,장우영,이순진,손희정,최규완,도재혁,백승운,이준행,이풍렬,이종철,박철근,임윤정,김갑철,김재준,임효근 대한소화기학회 2001 대한소화기학회지 Vol.37 No.3

Background & Aims: Gastrointestinal stromal tumor (GIST) constitutes primary nonepithelial neoplasms of the gastrointestinal tract and includes most tumors previously designated as leiomyomas/leiomyosarcomas. GIST is pathologically divided into benign, borderline and malignanct tumor. This study was carried out to delineate the clinical and pathologic features of GIST and to establish the features predicting malignancy. Methods: Clinicopathologic data of 84 patients who had GIST from 1995 to 1999 were reviewed. Results: The subjects were 42 men and 42 women, and their mean age was 59.3±13.2 years. We located fifty-two tumors in the stomach, 28 tumors in the small intestine and 4 tumors in the colorectum years. Histopathology revealed benign tumors in 23 of the 84 patients, borderline tumors in 22, malignant tumors in 39. The average size of tumors was 6.23 cm in diameter (range, 0.6-26 cm). Metastasis was noted in 15.5% of the patients at diagnosis. On endoscopy, ulceration was more common in malignancy. Endsonographic findings except size were not significant statistically. On contrast enhanced computed tomography, malignancy showed more irregular shape and variable attenuation than benign lesions, which seemed to be valuable factor. Conclusions: The variables predicting malignancy were large tumor size (≥5 cm), location of the colorectum, palpable abdominal mass, ulceration on endoscopy, and less uniformity in shape and variable attenuation on computed tomography.

DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

가상평가법(CVM)에 의한 도시공원의 관리운영에 대한 경제적 가치평가에 관한 연구 - 부산시 APEC기념공원을 대상으로 -

김영하,박승범,Kim, Yeong-Ha,Park, Seung-Burm 한국조경학회 2014 韓國造景學會誌 Vol.42 No.2

본 연구는 도시공원의 관리운영을 위한 민간기금 조성측면에서 부산시 APEC기념공원을 대상으로 가상평가법(Contingent Valuation Method: CVM)을 이용하여, 쾌적한 도시공원 유지를 위한 거주민이 인식하는 경제적 가치를 평가했다. 그 결과, 부산시 APEC기념공원의 관리운영을 위해 월 가구당 지불 가능한 추정금액은 5,673원에서 8,358원으로 평가되었다. 추정금액을 공원 유치권역의 가구로 확대하여 살펴보면, 2012년 편성된 공원 관리예산의 2.2~3.3배 수준으로 분석되어, 지역 거주민들이 인식하는 쾌적한 공원 유지를 위해 지불의사금액이 편성된 관리예산보다 높은 것으로 나타났다. 그리고 도시공원의 관리운영을 위한 지불의사금액에 영향을 주는 응답자의 사회적 요인으로는 연령, 교육수준, 소득으로 분석되었으며, 연령대가 높고, 교육수준이 높은 응답자에서 지불의사가 높은 것으로 평가되었다. 또한, 거주민의 공원이용 및 인식에 따라 도시공원 관리운영을 위한 지불의사금액에 많은 영향을 미치고 있는 것으로 나타났다. 특히 공원이용 빈도가 높은 거주민이 지불의사가 높으며, 주1회 이상 공원이용자가 전체 지불의사금액보다 1.1배 높은 추정금액으로 평가되었다. 그리고 공원의 필요성을 인식하는 것보다 공원을 만족하는 것이 더 높은 지불의사금액으로 이어지는 것을 밝혀냈다. 따라서 공원 만족도 제고를 위한 공원시설관리 및 이용프로그램 운영이 필요하며, 공원에 대한 인식을 기부로 연결할 수 있는 공원문화 보급정책이 필요하다고 판단된다. This study evaluated economic value recognized by the residents for the pleasant urban park management using contingent valuation method(CVM) to APEC Memorial Park in Pusan in terms of private fund development for the management of the city park. As a result, the estimated amount payable by each household for the management of APEC Memorial Park in Busan was between 5,673 won and 8,358 won. If such amount is expanded to households in park hosting area, it was 2.2~2.3 times of the park management budget in 2012, which demonstrates the willingness to pay (WTP) of residents for pleasant park was higher than the management budget. Social factors of respondents affecting WTP for city park management operation included age, educational level and income level. Those with high age and high education level had higher WTP. And, WTP was much affected by the use of park and recognition of residents. In particular, those with high frequency in the use of parks had higher WTP, and the users with more than once per week had 1.1 times higher WTP than overall WTP. Then, the satisfaction of park rather than recognition of necessity of park had led to higher WTP. Accordingly, it is necessary to manage park facility management and use program operation to enhance park satisfaction and it is also required to develop a park culture distribution policy to link such recognition to donation.

부산광역시 도시공원의 관리운영 실태 분석에 관한 연구 - 관리주체측면을 중심으로 -

김영하,안양욱,박승범,Kim, Yeong-Ha,An, Yang-Wook,Park, Seung-Burm 한국조경학회 2012 韓國造景學會誌 Vol.40 No.6

본 연구는 부산광역시 조성 완료된 근린공원 69개소를 대상으로 관리하는 운영주체에 대한 파악과 그 실태를 분석하는 것을 목적으로 하고 있다. 이러한 연구목적을 달성하기 위해 공원녹지 관련 업무자료 및 담당자와 면담, 그리고 도시공원의 예산편성 자료를 바탕으로 관리주체를 조사하였으며, 위탁관리 및 별도의 기관에서 관리되는 경우는 홈페이지 및 직접방문을 통한 자료 수집을 통해 연구를 진행했다. 공원의 관리방식은 자치단체의 본청과 사업소에서 직영관리가 48개소(69.6%), 공단 및 민간단체에 위탁관리가 18개소(26.1%), 직영관리와 위탁관리를 병행하는 혼합관리가 3개소(4.3%)로 파악되었다. 그리고 도시공원의 관리 실태를 종합분석한 결과, 위탁관리하고 있는 공원은 공원의 총괄 관리가 미약하며, 공원관리가 조경 및 청소 등의 유지관리 위주로 관리되어 이용자를 위한 프로그램 운영이 미흡한 것으로 나타났다. 그리고 기초자치단체에서 관리되고 있는 총 46개소의 공원은 대부분 규모가 작은 근린공원이며, 공원의 유지관리를 위해 무기계약직 근로자 및 기간제근로자를 고용하여 관리하고 있어, 공원 관리운영을 위한 전문 인력 및 조직체계가 개선이 시급한 것으로 분석되었다. 또한, 공원 내 교양시설은 별도의 기관에서 관리하고 있어, 공원 내 공원시설이 아닌 독립된 교양시설로서 공원관리와 분리된 형태로 관리되고 있는 것으로 나타났다. 이러한 결과의 해결 방안으로는 공원의 적정인원 고용에 대한 법제화 및 제도적 개선, 시민단체와의 협력적 네트워크 구축, 시대변화에 따른 이용프로그램 기획 및 개발과 더불어 이용객 위주의 프로그램 관리를 지속적으로 실시하여야 할 것으로 판단된다. This study aims to figure out the main agent of management in the 69 recently constructed neighborhood parks in Busan, and to analyze the present status of the main agents. For this purpose, the work resources on park and landscape management, interview to related staff, and the budget on urban parks were found as the main agents of management. In case the parks were managed by consignment or by other separate organization, this study collected resources through the homepage or personal visits. As a result for the management method on parks, about 48 parks(69.6%) were under direct management by the local governments' main office and its business offices. Eighteen parks(26.1%) were commissioned to corporation or private organizations and three parks(4.3%) were operated by both direct and commissioned management. Because of the overall management result on urban parks, the company under outsourced management is not sufficient for a comprehensive management. Such is mainly focused on the maintenance like landscape or cleaning, but have fewer programs for the users. Forty-six parks cared by the local governments are mainly small sized neighborhood parks. For the management, contract workers or short-term workers are hired. It demonstrates an urgent need to improve professional personnel and organizational system for park management. In addition, any educational or cultural facility in the park is managed by separate institutions. Thus, it is not controlled as a park facility but an independent facility with separate controls. Moreover, to solve such problems, it needs legalization on the proper employment for parks, institutional improvement, cooperative network with NGO, planning and development of the program used according to the change of time, and customer oriented program management.