Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

류마티스관절염과 만성 호산구성 폐렴에 동반된 호산구성 흉막삼출

지용관 ( Yong Gwan Jee ),라상호 ( Sang Ho Ra ),박유미 ( Yu Mi Park ),차재황 ( Jae Whang Cha ),강용석 ( Yong Seok Kang ),박정하 ( Jeong Ha Park ),강태영 ( Tae Young Kang ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.5

We describe a 48-year-old man with family history of rheumatoid arthritis (RA) affected by chronic eosinophilic pneumonia (CEP) with severe peripheral eosinophilia. CEP might develop as a complication of longstanding active RA. The patient with 5 months history of seropositive RA and chronic respiratory symptoms, alveolar and blood eosinophilia, peripheral pulmonary infiltrates and pleural effusion on chest imaging. The lung may be involved as an extraarticular manifestation of RA. However, CEP is not recognized as a typical lung manifestation of RA, and the two diseases rarely coexist. The effusion was an eosinophil predominant exudates and was characterized by low pH, and glucose level and high lactic dehydrogenase. The patient responded rapidly to combination of steroids and disease modifying anti-rheumatic drugs.

3′‐Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis

Kang, Li‐,Jung,Kwon, Eun‐,Soo,Lee, Kwang Min,Cho, Chanmi,Lee, Jae‐,In,Ryu, Young Bae,Youm, Tae Hyun,Jeon, Jimin,Cho, Mi Ra,Jeong, Seon‐,Yong,Lee, Sang‐,Rae,Kim, Wook,Yang John Wiley and Sons Inc. 2018 British journal of pharmacology Vol.175 No.23

<P><B>Background and Purpose</B></P><P>3′‐Sialyllactose (3′‐SL) is a safe compound that is present in high levels in human milk. Although it has anti‐inflammatory properties and supports immune homeostasis, its effect on collagen‐induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3′‐SL on the progression of rheumatoid arthritis (RA) in <I>in vitro</I> and <I>in vivo</I> models.</P><P><B>Experimental Approach</B></P><P>The anti‐arthritic effect of 3′‐SL was analysed with fibroblast‐like synoviocytes <I>in vitro</I> and an <I>in vivo</I> mouse model of CIA. RT‐PCR, Western blotting and ELISA were performed to evaluate its effects <I>in vitro</I>. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin‐O and haematoxylin staining.</P><P><B>Key Results</B></P><P>3′‐SL markedly alleviated the severity of CIA in the mice by reducing paw swelling, clinical scores, incidence rate, serum levels of inflammatory cytokines and autoantibody production. Moreover, 3′‐SL reduced synovitis and pannus formation and suppressed cartilage destruction by blocking secretion of chemokines, pro‐inflammatory cytokines, https://en.wikipedia.org/wiki/Matrix_metalloproteinases and osteoclastogenesis <I>via</I> NF‐κB signalling. Notably, phosphorylation of p65, which is a key protein in the NF‐κB signalling pathway, was totally blocked by 3′‐SL in the RA models.</P><P><B>Conclusions and Implications</B></P><P>3′‐SL ameliorated pathogenesis of CIA by suppressing catabolic factor expression, proliferation of inflammatory immune cells and osteoclastogenesis. These effects were mediated <I>via</I> blockade of the NF‐κB signalling pathway. Therefore, 3′‐SL exerted prophylactic and therapeutic effects and could be a novel therapeutic agent for the treatment of RA.</P>

복부비만 여성 대상 걷기 운동 프로그램의 비만관련 지표 및 심폐체력 개선 효과

이미라,김완수 김천과학대학 2007 김천과학대학 논문집 Vol.33 No.-

The purpose of this study was to investigate the effects of a 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, on obesity-related indices (body weight, body mass index, waist circumference, waist-to-bip ratio, body fat percent, and total body fat) and aerobic fitness in Korean women with abdominal obesity. The subjects were 11 middle-aged women with abdominal obesity (waist circumference ≥80 cm). The intervention consisted of walking for 45-60 min per day at an intensity of 40(50) to 60(65)% of heart rate reserve(HRR) for 3 days per week Subjects of this study had decreases in obesity-related indices overall. Decreases in body weight, waist-to-hip ratio, body mass index, waist circumference, and total body fat were statistically significant. Aerobic fitness was increased after the intervention and this increase was close to statistical significance. A 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, could be an effective strategy to reduce obesity-related indices in middle-aged Korean women with abdominal obesity. Whether higher amount of exercise is required to promote significant improvement in aerobic fitness in this population needs to be investigated in well-controlled further studies.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

동북아 협력 네트워크와 지방의 역할

라미경 호남대학교 인문사회과학연구소 2007 인문사회과학연구 Vol.17 No.-

국제환경의 변화에 따라 동북아 역내 간에 있어서 긴밀한 교류 협력의 필요성은 더욱 절실하게 제기되고 있다. 더 나아가 동북아 지역의 시민들이 공동의 평화와 번영을 함께 추구하고 영위할 수 있는 지역공동체의 설립이 중요한 과제로 떠오르고 있다. 이에 본 연구는 동북아 지역공동체 형성이라는 상황을 지방간의 관계를 중심으로 분석하고자 한다. 동북아 지역의 지방들이 국경을 넘어 어떻게 교류협력하고 있는가,서로 쟁점들은 무엇인가 그리고 경제적 상호보완성과 지리적 근접성을 살리면서 앞으로 지역공동체 형성에 지방들이 기여할 수 있을 것인가 등에 대한 연구는 시의적절하다고 생각한다. 왜냐하면 동북아 지역공동체와 이 지역의 협력이 중앙정부 차원에서만 진행되고 있지 않기 때문 이다. 또한 지방끼리의 협력과 전망에 대한 연구 성과가 미비하기 때문에 본 연구를 통해 이 분야연구에 기여하고자 하는 데 목적이 있다. This paper discusses the Roles of Local Government and Cooperative Network In Northwest Asia. It follows in change of international environment and it is proposed the necessity of the cooperation to Northeast Asia inside of area. The research which is a Northeast Asia area community it analyzes the relationship of region for in the center and it does to sleep. The regions of the Northeast Asia area will go over the border and the flow cooperative, with each other are the issues will be what how and to save an economic trade name complement result geography neighboring characteristic and the regions will be able to contribute in the next area community formation is the research against the back thinks that it is right time and appropriate. Because the Northeast Asia area community and cooperation of this region only central government dimension, it is because is not advanced.

The efficacy of bench-step aerobic program in reducing some cardiovascular risk factors in overweight or obese women

이미라,김완수 대구대학교 인문과학연구소 2008 人文科學硏究 Vol.32 No.-

본 연구의 목적은 12주 스텝운동 프로그램이 과체중(23 ㎏/㎡≤체질량지수<25 ㎏/㎡) 및 비만(체질량지수≥25 ㎏/㎡) 여성의 심혈관 위험 요인(비만관련 지표와 혈당 및 지질성분)에 미치는 효과를 알아보기 위한 것이다. 선발된 25명의 과체중 및 비만 여성 중 프로그램에 90% 이상 참여하고 프로그램 전과 후에 이루어진 검사를 수행한 15명의 프로그램 수행 전과 후의 비만관련 지표(체중, 체질량지수, 체지방율 및 총체지방), 혈당, 중성지방 그리고 고밀도지단백콜레스테롤을 비교하였다. 본 프로그램은 주 3회, 12주 동안 이루어졌고, 45분에서 60분 동안 예비심박수의 40/50%에서 50/60%의 운동 강도로 진행되었다. 운동량은 건강상의 이점 획득 목적으로 현재 권고되는 최소 운동량에 근거하여 결정되었다. 12주 스텝운동 프로그램 후 대상자의 체지방율이 유의수준에 가깝게 감소되었고(T=2.121, P=.052), 체중, 체질량지수와 체지방이 유의하게 감소되어(T=5.270, P=.000; T=2.658, P=.019; T=3.661, P=.003), 전반적으로 비만관련 지표에 미치는 효과가 지지되었다. 혈당(T=1.850, P=.087), 중성지방(T=-1.249, P=.234) 및 고밀도지단백콜레스테롤(T=.084, P=.934)은 유의한 변화를 보이지 않았다. 본 연구결과는 선행 연구결과들과 함께 12주 스텝운동 프로그램이 전반적으로 비만관련 지표를 유의하게 감소시킴으로써 과체중 및 비만 여성의 심혈관 질환 위험을 감소시킬 수 있는 효과적인 운동 프로그램이 될 수 있음을 보여주고 있다. 반면 혈당과 혈중 지질성분 개선을 위해서는 운동량의 증가 또는 근량 증가 및 내장지방 감소 효과를 초래할 수 있는 운동 프로그램으로의 수정 등과 같은 전략들이 부가되어야 한다는 것을 시사하고 있다. 그러나 본 연구 포함 스텝운동 프로그램의 심혈관 위험 감소 효과를 검증한 현재까지의 연구들은 연구 설계상의 제한점을 갖고 있고, 따라서 일반화를 위한 무작위화되고 통제된 연구가 필요하다.

발달지체유아의 유치원 전환과 적응에 관한 연구

강미라 한국특수아동학회 2003 특수아동교육연구 Vol.5 No.2

이 연구에서는 유아들의 성공적인 유치원 전환에 관한 관련 문헌과 선행연구를 고찰함과 동시에 유치원 전환 후의 적응에 대해 부모와 교사를 대상으로 인식 수준을 조사하였다. 구체적인 연구의 목적은 첫째, 유아기 전환의 용어에 대한 정의와 성공적인 전환의 목적을 구명하고, 둘째, 유아들의 유치원 전환 촉진 전략을 제시하며, 셋째, 유아들의 유치원 전환 이후의 적응에 대해 부모와 교사의 인식 수준을 밝힌다. 유아의 유치원으로의 전환에 관한 연구에서 얻은 결론은 첫째, 초기 아동기의 전환은 유아와 가족에게 큰 변화를 초래할 수 있는데, 이 시기에는 세심한 전환계획이 필요하며, 성공적인 전환의 목적은 긍정적인 전환 경험이 청소년기와 성인기로의 성공적인 전환으로 연결된다. 둘째, 유치원으로의 전환을 촉진하기 위한 전략에는 실제 이동이 일어나기 6개월∼1년 전에 준비단계로서 개별적으로 전환 계획을 수립하고, 전환을 이행한 후 적응상의 장애를 최소화하기 위한 추후지도 단계까지를 제시하고 있다. 셋째, 유아의 유치원 적응을 돕기 위해서는 부모와 교사의 의사소통이 필요하다. 그리고 마지막으로, 모든 유아들의 성공적인 유치원 전환 연구와 관련하여 후속연구를 위한 제언을 제시한다. Transition to kindergarten is a very natural change in the early years. But, entering a new program is usually stressful for children with/without developmental delayed and their families. The purpose of this study is to describe and explain successful transition process and strategies for children who move from preschool programs into kindergartens, and is to investigate the level of perception of parents and teachers on early children' adjustment after finished transition to kindergarten. In this study, methods of research are to overview the related literature study and researches which is done before for a successful transition of children, and to analyze of data, ANOVAs were performed to examine the level between parents and teachers perception on adjustment. The results derived from related literature study and research done before regarding kindergarten transition are as follows. Fist, although transition services are focused on the transition of society through graduation of high school, early childhood transition describes the process of transition from a preschool educational environment to a kindergarten educational process. Second, The transition strategies shows the temporary influence of what can be seen as an important factor in deciding the quality of the appropriate transition experience for development. The basic aspect of this strategy is that it includes the child, family, and community in the child's transition. In the ecological approach, the child is considered a member of the family and consider as a part of cultural community and a more broad society. Third, on the perception level of child's adjustment in the kindergarten, in case of parents, parent group with early children without developmental delay who have a high level of participation, while children with developmental delay parent group have a low level of participation. and in case of teachers, teacher group who have been three year education career have a high perception level of child's adjustment, while teacher group who have been below one year education career have a low perception of child's adjustment in the kindergarten. Finally, the interaction between one of the child's many present environment and transitioning environment can help child adjusts to the new program. Here following the agreement, the plan developed for the individual family and cooperation is suggested to the institution and service organization.

유치원 전환에 대한 부모․교사의 참여와 만족에 관한 인식 조사

강미라 한국특수교육문제연구소 2003 특수교육저널 : 이론과 실천 Vol.4 No.4

본 연구에서는 발달지체유아를 포함하여 모든 유아들의 유치원 전환을 보다 성공적으로 실행하는데 도움을 주고자 부모와 조기(특수)교사, 그리고 유치원 교사의 유치원 전환에 대한 참여도와 만족도를 밝히고자 하였다. 본 연구의 조사대상자는 특수학급이 있는 초등학교의 병설 유치원과 통합교육을 실시하고 있는 사립유치원, 특수학교 유치부의 담당교사와 부모로 선정하였으며, 사용된 조사도구는, 「유치원 전환의 참여와 만족」에 대한 문항으로, 분석방법은 문항별로 평균과 표준편차를 산출하여 그 수준을 밝히고, 부모와 교사의 변인간 참여 수준과 만족 수준에서 유의차를 알아보기 위해 F검정(변량분석)을 실시하였다. 연구의 결과에서는 자녀의 유치원 전환에 대한 참여 수준이 높은 발달지체유아 부모 집단에서는 오히려 전환과정에서의 만족 수준이 가장 낮은 것으로 나타났다. 그리고 교사집단에서는 교육경력이 3년 이상 되는 교사 집단의 참여와 만족 수준이 가장 높았고, 받는 팀의 1년 미만인 교사 집단의 참여와 만족 수준이 가장 낮은 것으로 나타났다. 조기교육기관의 지원에도 불구하고 부모들은 아직도 자녀의 유치원 전환에 대해 많은 어려움을 느끼고 있는 것으로 나타났는데, 이것은 유아들이 취원전 교육 서비스에서 유치원의 교실과 교육과정으로 순조롭게 전환하도록 보내는 팀과 받는 팀간의 연계와 협력이 필요함을 시사하고 있다. The study is to investigate the level of perception of participation, satisfaction of parents and teachers including early childhood special education and kindergarten teacher in order to kindergarten transition of children. The specific purpose of this study are as fellows; First, is to find out the level of perception of parents and teachers' participation on kindergarten transition. Seconder, is revel the level of perception of parents and teachers' satisfaction on kindergarten transition. To analyze of data, ANOVAs were performed to examine the level between parents and teachers perception on kindergarten transition as part of participation and satisfaction. The conclusion drawn from the results and discussion of the study was as fellows; First, on the level of participation on the process of transition to kindergarten, in case of parents, parents group with early children with developmental delay who attend at concurrent establishment kindergarten have a high level of participation, while general parent group with early children without developmental delay who attend at concurrent establishment kindergarten have a low level of participation. and in case of teachers, of STM(sending team members), teacher group who have been three year education career have a high perception level of participation, while teacher group who have been below one year education career have a low perception of participation. Second, on the level of satisfaction on the process of transition to kindergarten, in case of parents, parent group with early children with developmental delay who attent at private establishment kindergarten have a high preception level of satisfaction, while parents group with early children with developmental delay who attent at kindergarten class in special school for the handicapped have low perception level of it. and in case of teachers, of RTM(Receiving Team Members), teacher group who have been over three years education career have a high level of it, while teacher group who have been below one year education career have a low level of it. The suggestion drawn from the conclusion in the study was as fellows; First, The further study have a necessity of study on the effect of transition in education settings after leaving kindergarten. Second, The further study have a necessity of case study on inter-agencies to be needed cooperation of education agencies, and counseling of parents and teachers, communication․cooperation between STM(Sending Team Members) and RTM(Receiving Team Members). Third, The further study have a necessity of study on transition to kindergarten as subjects personnels who are education administrator, and parents and facility in special schools for early children with developmental delay excluded in this study. Fourth, The following study have a necessity of study on development of korean transition model to kindergarten.