Effect of aberrantly methylated androgen receptor target gene PCDH7 on the development of androgen-independent prostate cancer cells

Siqi Xu,Xiaoyan Wu,Zhihua Tao,Hongsheng Li1,Chenliang Fan,Songjin Chen,Jianwei Guo,Yao Ning,Xuqi Hu 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Androgen-independent prostate cancer (AIPC) is an extremely malignant tumor developed from the androgen dependent (ADPC). However, the mechanism of transition process from ADPC to AIPC remains unknown. Objective Here we aimed to identify the androgen receptor (AR) target gene and its roles in AIPC. Methods Target genes of AR were identified by ChIP-seq in AIPC cells. AR target gene PCDH7 was detected by real time PCR and western blot. Methylation of PCDH7 was measured by bisulfite sequencing and bisulfite amplicon sequencing. Cell growth, invasion and apoptosis were measured by CCK-8, transwell and flow cytometry, respectively. Results AR was significantly enriched in the upstream of PCDH7 gene. The expression of PCDH7 was significantly decreased, while the methylation of PCDH7 was increased in the AIPC cells compared to the ADPC cells. DNA methyltransferase inhibitor significantly suppressed the methylation and increased the mRNA and protein level of PCDH7. Moreover, overexpression of DNMT1 remarkably reduced the mRNA and protein level of PCDH7. DNA methyltransferase inhibitor decreased the cell growth and invasion while promote the cell apoptosis in the AIPC cells. AR significantly target PCDH7, whose hypermethylation may repress cell growth and invasion, and promote apoptosis in AIPC. Conclusions This study might provide a novel potential target for the treatment of AIPC.

Hardening and toughening mechanisms in nanotwinned ceramics

Li, Xiaoyan,Yin, Sheng,Oh, Sang Ho,Gao, Huajian Elsevier 2017 Scripta materialia Vol.133 No.-

<P><B>Abstract</B></P> <P>Recent experiments have reported that nanotwinned ceramics with covalent or ionic bonds exhibit unprecedented hardness, enhanced fracture toughness and improved thermal stability. In this viewpoint paper, we highlight some preliminary studies on mechanical properties of nanotwinned ceramics either synthesized in the laboratory or extracted from biological materials, with an emphasis on their hardening and toughening mechanisms from the point of view of defects and bonding structures. Some discussions are also made on possible future research directions, such as exploring underlying deformation mechanisms via in situ TEM testing and large-scale atomistic simulations, and investigating the twin size and orientation effects.</P>

LncRNA XIST promotes carboplatin resistance of ovarian cancer through activating autophagy via targeting miR-506-3p/FOXP1 axis

Xiaoyan Xia,Zikui Li,Yaojun Li,Feng Ye,Xiaoming Zhou 대한부인종양학회 2022 Journal of Gynecologic Oncology Vol.33 No.6

Objective: Resistance to chemotherapy drugs makes ovarian cancer (OC) difficult to treat and ultimately kills patients. Long non-coding RNAs are closely related to carboplatin resistance in OC. In present study, we explored the role of lncRNA X-inactive specific transcript (XIST) on carboplatin resistance in OC. Methods: Cell viability, proliferation, and apoptosis were assessed through 2,5-diphenyl- 2H-tetrazolium bromide, colony formation, and flow cytometry assays, respectively. Microtubule-associated protein 1A/1B-light chain 3 expression was evaluated by immunofluorescence assay to analyze the cell autophagy. The interaction of XIST/ miR-506-3p or miR-506-3p/forkhead box protein P1 (FOXP1) was analyzed using RNA immunoprecipitation (RIP) and dual-luciferases reporter assays. The function of XIST/ miR-506-3p/FOXP1 axis in vivo was further confirmed by tumor xenograft study and immunohistochemistry. Results: The expression of XIST and FOXP1 increased while miR-506-3p decreased in OC and carboplatin resistance cells. XIST silencing repressed the proliferative and autophagic capacities of carboplatin resistance cells while promoted the apoptosis. XIST overexpression led to the opposite results. XIST targeted miR-506-3p and downregulated its expression. MiR-506-3p inhibition facilitated the proliferative and autophagic capacities while suppressed the apoptosis of cells, XIST knockdown reversed these effects. MiR-506-3p bound to FOXP1. XIST knockdown or miR-506-3p overexpression reversed the increase of cell proliferative and autophagic abilities and the decrease of apoptosis rate induced by FOXP1 overexpression. XIST affected autophagy and carboplatin resistance in vivo via regulating the miR-506-3p/FOXP1 axis. Conclusion: XIST knockdown inhibited autophagy and carboplatin resistance of OC through FOXP1/protein kinase B (AKT)/mammalian target of rapamycin pathway by targeting miR-506-3p.

MOF-derived nitrogen-doped porous carbon as metal-free catalysts for acetylene hydrochlorination

Xiaoyan Li,Jinli Zhang,Wei Liu 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.44 No.-

A series of ZIF-derived nitrogen-doped carbon materials were prepared using melamine as the additionalnitrogen sources for acetylene hydrochlorination. The results indicated that the optimal complex of Z4M1exhibits the highest acetylene conversion of 60% under the reaction conditions of 180 C, C2H2 gas hourlyspace velocity (GHSV) = 50 h 1, VHCl/VC2H2 = 1.15, suggesting a promising metal-free catalyst for acetylenehydrochlorination. It is illustrated that the additions of melamine not only make the complex of Z4M1displays special morphological structure and proper pore structure, but also adjust the relative contentratio of three nitrogen species in ZIF-derived nitrogen-doped carbon materials.

Identification and Screening of Gene(s) Related to Susceptibility to Enterotoxigenic Escherichia coli F4ab/ac in Piglets

Li, Hejun,Li, Yuhua,Qiu, Xiaotian,Niu, Xiaoyan,Liu, Yang,Zhang, Qin Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.4

In 2004, Jorgensen and coworkers proposed the MUC4 gene as a candidate gene of enterotoxigenic Escherichia coli (ETEC) F4ab/ac receptor in piglets and a mutation of $G{\rightarrow}C$ in intron 7 of MUC4 was identified to be associated with the ETEC F4ab/ac adhesion phenotypes. In this study, we used 310 piglets of three breeds (Landrace, Large White and Chinese Songliao Black) to analyze the relationship between this mutation and the F4ab/ac adhesion phenotype. The results show that the genotypes at this site and the ETEC F4ab/ac adhesion phenotypes were not completely consistent, although they are very strongly associated. Among the individuals with genotype CC, which was identified as a resistant genotype to F4ab/ac adhesion, only 72.1% (124/172) were non-adhesive to ETEC F4ab and 77.9% (134/172) were non-adhesive to ETEC F4ac infections. This suggests that this mutation may not be the causative mutation for ETEC F4ab/ac adhesion, rather, the actual causative mutation may be in another gene closely linked to MUC4, or at aother site within the MUC4 gene. Our results also suggest that the receptors of F4ab and F4ac may be determined by two different but closely linked loci. In order to screen other genes related to F4ab/ac adhesion in piglets, the mRNA profiles from six full sib piglets, of which three were adhesive to ETEC F4ab/ac and three non-adhesive, were analyzed by suppression subtractive hybridization (SSH). One up-regulated gene, Ep-CAM, was selected for further analysis based on its role in the intestinal epithelial cells adhesion. Using real-time RT-PCR, we found that the Ep-CAM gene was significantly up-regulated in the piglets adhesive to F4ab/ac. It was mapped to SSC3q11-q14 by radiation hybrid mapping.

Comparative Transcriptome Analysis of Cynanchum thesioides Under Drought Stress Reveals Candidate Genes Involved in Succinic Acid Biosynthesis

Xiaoyan Zhang,Fenglan Zhang,Zhi Li,Zhongren Yang,Lizhen Hao,Hongyu Zhao 한국식물학회 2023 Journal of Plant Biology Vol.66 No.3

Cynanchum thesioides, a member of the Asclepiadaceae family, are plants that have been used in traditional Chinese medicine and their latex contains succinic acid which has an important medicinal value. To identify candidate genes involved in succinic acid biosynthesis and accumulation, we analyzed transcriptome data from C. thesioides under drought stress using Illumina sequencing technology. A total of 146 and 172 unique genes were annotated as encoding enzymes involved in the succinic acid biosynthesis of C. thesioides and C. thesioides var. australe and the number of differentially expressed genes (DEGs) was 40 and 32, respectively. As drought stress increased, nine genes were significantly up-regulated and seven genes were significantly down-regulated in C. thesioides. In C. thesioides var. australe seven genes were up-regulated and nine were down-regulated. Enzymes participating in succinic acid biosynthesis were ATP citrate (pro-S)-lyase, succinate dehydrogenase, aconitate hydratase, 2-oxoglutarate dehydrogenase, citrate synthase, glutamate synthase, fumarate hydratase, succinyl-CoA synthetase, succinate-semialdehyde dehydrogenase, glutamate dehydrogenase, isocitrate dehydrogenase, 4-aminobutyrate transaminase, malate dehydrogenase, and glutamate decarboxylase and their activities relating to succinic acid synthesis were determined. RNA-seq sequencing was used in our study. The genes involved in biosynthesis and accumulation of succinic acid in C. thesioides were predicted. Succinic acid content was positively correlated with MDH (BMK_Unigene_033366), but negatively correlated with GAD (BMK_Unigene_096180 and BMK_Unigene_030468), SDH (BMK_Unigene_142841). These results provide an important resource for further research on succinic acid of C. thesioides and other homologous species.

Photocatalyst TiO2/WO3/GO nano-composite with high efficient photocatalytic performance for BPA degradation under visible light and solar light illumination

Xiaoyan Hao,Man Li,Li Zhang,Kuokuo Wang,Chunguang Liu 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.55 No.-

A high efficient photocatalyst (TiO2/WO3/GO) was developed with tungsten trioxide doping and graphene oxide hybridization. XRD and SEM indicate the formation of crystalline anatase/rutile TiO2 and hexagonal and cubic WO3. UV–vis spectroscopy shows the excellent visible-light-responsive performance. The photocatalyst exhibits excellent photocatalytic performance for bisphenol A degradation under visible light and sunlight irradiations. Transformation by-products were identified and main degradation pathways were proposed. The photocatalytic mechanism is also proposed on the basis of the fact that charge separation is facilitated owing to photo-generated electrons transfer from TiO2 to WO3 to GO and photogenerated holes transfer from WO3 to TiO2.

Baseline Total Metabolic Tumor Volume and Total Lesion Glycolysis Measured on 18F-FDG PET-CT Predict Outcomes in T-Cell Lymphoblastic Lymphoma

Xiaoyan Feng,Xin Wen,Ling Li,Zhenchang Sun,Xin Li,Lei Zhang,Jingjing Wu,Xiaorui Fu,Xinhua Wang,Hui Yu,Xinran Ma,Xudong Zhang,Xinli Xie,Xingmin Han,Mingzhi Zhang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.3

Purpose There is no optimal prognostic model for T-cell lymphoblastic lymphoma (T-LBL). Here, we discussed the predictive value of total metabolic tumor volume (TMTV) and total lesion glycolysis (TLG) measured on 18F-fluorodeoxyglucose positron emission tomography–computed tomography (PET-CT) in T-LBL.Materials and Methods Thirty-seven treatment naïve T-LBL patients with PET-CT scans were enrolled. TMTV was obtained using the 41% maximum standardized uptake value (SUVmax) threshold method, and TLG was measured as metabolic tumor volume multiplied by the mean SUV. Progression-free survival (PFS) and overall survival (OS) were analyzed by Kaplan-Meier curves and compared by the log-rank test.Results The optimal cutoff values for SUVmax, TMTV, and TLG were 12.7, 302 cm3, and 890, respectively. A high SUVmax, TMTV, and TLG indicated a shorten PFS and OS. On multivariable analysis, TMTV ≥ 302 cm3, and central nervous system (CNS) involvement predicted inferior PFS, while high SUVmax, TLG and CNS involvement were associated with worse OS. Subsequently, we generated a risk model comprising high SUVmax, TMTV or TLG and CNS involvement, which stratified the population into three risk groups, which had significantly different median PFS of not reached, 14 months, and 7 months for low-risk group, mediate-risk group, and high-risk group, respectively (p < 0.001). Median OS were not reached, 27 months, and 13 months, respectively (p < 0.001).Conclusion Baseline SUVmax, TMTV, and TLG measured on PET-CT are strong predictors of worse outcome in T-LBL. A risk model integrating these three parameters with CNS involvement identifies patients at high risk of disease progression.

Effect of Red Pepper (Capsicum frutescens) Powder or Red Pepper Pigment on the Performance and Egg Yolk Color of Laying Hens

Li, Huaqiang,Jin, Liji,Wu, Feifei,Thacker, Philip,Li, Xiaoyu,You, Jiansong,Wang, Xiaoyan,Liu, Sizhao,Li, Shuying,Xu, Yongping Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.11

Two experiments were conducted to study the effects of red pepper (Capsicum frutescens) powder or red pepper pigment on the performance and egg yolk color of laying hens. In Exp. 1, 210, thirty-wk old, Hy-line Brown laying hens were fed one of seven diets containing 0.3, 0.6, 1.2, 2.0, 4.8 or 9.6 ppm red pepper pigment or 0.3 ppm carophyll red. Each diet was fed to three replicate batteries of hens with each battery consisting of a row of five cages of hens with two hens per cage (n = 3). In Exp. 2, 180, thirty-wk old, Hyline Brown laying hens, housed similarly to those in Exp. 1, were fed an unsupplemented basal diet as well as treatments in which the basal diet was supplemented with 0.8% red pepper powder processed in a laboratory blender to an average particle size of $300{\mu}m$, 0.8% red pepper powder processed as a super fine powder with a vibrational mill ($44{\mu}m$) and finally 0.8% red pepper powder processed as a super fine powder with a vibrational mill but mixed with 5% $Na_2CO_3$ either before or after grinding. A diet supplemented with 0.3 ppm carophyll red pigment was also included (n = 3). In both experiments, hens were fed the red pepper powder or pigment for 14 days. After feeding of the powder or pigment was terminated, all hens were fed the basal diet for eight more days to determine if the dietary treatments had any residual effects. In Exp. 1, there were no differences in egg-laying performance, feed consumption or feed conversion ratio due to inclusion of red pepper pigment in the diet. Average egg weight was higher (p<0.05) for birds fed 1.2, 2.4 or 9.6 ppm red pepper pigment than for birds fed the diet containing 0.3 ppm red pepper pigment. On d 14, egg color scores increased linearly as the level of red pepper pigment in the diet increased. In Exp. 2, feeding red pepper powder did not affect egg-laying performance, feed consumption or feed conversion ratio (p>0.05). However, compared with the control group, supplementation with all of the red pepper powder treatments increased egg weight (p<0.05). All the red pepper powder treatments also increased (p<0.05) the yolk color score compared with the control. The results of the present study suggest that both red pepper powder and pigment are effective feed additives for improving egg yolk color for laying hens.

Urinary Biomarkers for the Noninvasive Detection of Gastric Cancer

Li Dehong,Yan Li,Lin Fugui,Yuan Xiumei,Yang Xingwen,Yang Xiaoyan,Wei Lianhua,Yang Yang,Lu Yan 대한위암학회 2022 Journal of gastric cancer Vol.22 No.4

Gastric cancer (GC) is associated with high morbidity and mortality rates. Thus, early diagnosis is important to improve disease prognosis. Endoscopic assessment represents the most reliable imaging method for GC diagnosis; however, it is semi-invasive and costly and heavily depends on the skills of the endoscopist, which limit its clinical applicability. Therefore, the search for new sensitive biomarkers for the early detection of GC using noninvasive sampling collection methods has attracted much attention among scientists. Urine is considered an ideal biofluid, as it is readily accessible, less complex, and relatively stable than plasma and serum. Over the years, substantial progress has been made in screening for potential urinary biomarkers for GC. This review explores the possible applications and limitations of urinary biomarkers in GC detection and diagnosis.