Cloning, Expression, and Characterization of Protease-resistant Xylanase from Streptomyces fradiae var. K11

( Ning Li ),( Pei Long Yang ),( Ya Ru Wang ),( Hui Ying Luo ),( Kun Meng ),( Ning Feng Wu ),( Yun Liu Fan ),( Bin Yao ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.3

Utilization of Three Bark Extractives as Natural Photostabilizers for the Photostabilization of Wood Flour/Polypropylene Composites

Yao Peng,Ning Yan,Jinzhen Cao 한국섬유공학회 2020 Fibers and polymers Vol.21 No.7

Three kinds of tree bark extractives from trembling aspen (Populus tremuloides) (AE), lodgepole pine (Pinuscontorta) (LE), and western red cedar (Thuja plicata) (RE) were produced and used as antioxidants. The antioxidationactivities of those extractives were evaluated and compared with commercial antioxidant butylated hydroxytoluene (BHT). The obtained extractives were applied as natural photostabilizers to produce wood flour/polypropylene (WF/PP) compositesat an addition level of 2 wt%. The composites were subjected to QUV accelerated weathering tester for a total of 1200 h. Thechanges in surface color, surface morphology, and surface chemistry of composites were investigated during the exposure. The results showed that all bark extractives alleviated the photodegradation of composites successfully. Among them, REwas proved to be the most effective. It exhibited excellent UV absorption and similar free-radical inhibition ability comparedwith BHT. Consequently, composites containing RE exhibited less discoloration, less severe surface cracking, and lesschanges in surface chemistry during the whole weathering process. Overall, these results revealed that RE had great potentialas a natural photostabilizer in polymer systems.

An Updated Pooled Analysis of Glutathione S-transferase Genotype Polymorphisms and Risk of Adult Gliomas

Yao, Lei,Ji, Guixiang,Gu, Aihua,Zhao, Peng,Liu, Ning Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

Objective: Glutathione S-transferases (GSTs) are multifunctional enzymes that play a crucial role in the detoxification of both the endogenous products of oxidative stress and exogenous carcinogens. Recent studies investigating the association between genetic polymorphisms in GSTs and the risk of adult brain tumors have reported conflicting results. The rationale of this pooled analysis was to determine whether the presence of a GST variant increases adult glioma susceptibility by combining data from multiple studies. Methods: In our meta-analysis, 12 studies were identified by a search of the MEDLINE, HIGHWIRE, SCIENCEDIRECT and EMBASE databases. Of those 12, 11 evaluated GSTM1, nine evaluated GSTT1 and seven evaluated GSTP1 Ile105Val. Between-study heterogeneity was assessed using ${\chi}^2$-based Q statistic and the $I^2$ statistic. Crude odds ratios (ORs) with corresponding 95% confidence intervals (CIs) were used to estimate the association between GSTM1, GSTT1 and GSTP1 polymorphisms and the risk of adult gliomas. Results: The quantitative synthesis showed no significant evidence to indicate an association exists between the presence of a GSTM1, GSTT1 or GSTP1 Ile105Val haplotype polymorphism and the risk of adult gliomas (OR, 1.008, 1.246, 1.061 respectively; 95% CI, 0.901-1.129, 0.963-1.611, 0.653-1.724 respectively). Conclusions: Overall, this study did not suggest any strong relationship between GST variants or related enzyme polymorphisms and an increased risk of adult gliomas. Some caveats include absence of specific raw information on ethnic groups or smoking history on glioma cases in published articles; therefore, well-designed studies with a clear stratified analysis on potential confounding factors are needed to confirm these results.

Up-regulation of miR-106a targets LIMK1 and contributes to cognitive impairment induced by isoflurane anesthesia in mice

Ning Zhang,Weiguang Ye,Tianlong Wang,Hui Wen,Lan Yao 한국유전학회 2020 Genes & Genomics Vol.42 No.4

Background Postoperative cognitive dysfunction (POCD) had a great relationship with anesthesia during surgery, and miRNAs have been found involved in anesthesia-induced cognitive impairment. Objective To explore the role and potential mechanism of miR-106a in isoflurane anesthesia-induced cognitive impairment. Methods Adult male mice were treated with isoflurane anesthesia; Morris water maze tests and fear conditioning tests were performed; and expression levels of miR-106a and LIMK1 were determined by quantitative real-time PCR (qRT-PCR) and western blot. Dual luciferase reporter assay was used to determine the binding of miR-106a and 3’UTR of LIMK1. To verify the role of miR-106a, antagomir of miR-106a were intrahippocampally injected. Finally, expression of BCL2 apoptosis regulator (Bcl-2), LIM domain kinase 1 (LIMK1), BCL2-associated X, apoptosis regulator (Bax) and cleaved caspase3 was determined by western blot. Results In isoflurane anesthesia-treated group (IS), the percentage of target quadrant dwell time was significantly lower and the escape latency was significantly higher than in the control group (sham), and the freezing behavior of IS was significantly less in contextual fear conditioning tests. Expression levels of miR-106a were increased and those of LIMK1 were decreased in response to IS. Dual luciferase reporter assay showed that miR-106a could bind with the 3’UTR of LIMK1. Decreased expression levels of miR-106a improved the cognitive impairment of the mice treated with isoflurane. Intrahippocampally injected antagomir of miR-106a also increased LIMK1 and Bcl-2 levels, decreased the BAX and cleaved caspase3 expression levels in the mice treated with isoflurane. Conclusion Decrease of LIMK1 expression by miR-106a played an important role in isoflurane anesthesia-induced cognitive impairment.

Effect of post-annealing conditions on electrical and optical properties of AZO thin film on flexible PET substrates

Wenhao YAO,Kun LIU,Shulei CHEN,Xiaojie LIU,Dongyang WANG,Yingchao NING,Dechun BA,Guangyu DU,Yaoshuai BA 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Thermodynamic simulation and structural optimization of the collimator in the drift duct of EAST-NBI

Tang Ning,Hu Chun-dong,Xie Yuan-lai,Wei Jiang-long,Cui Zhi-Wei,Xie Jun-Wei,Pan Zhuo,Jiang Yao 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.11

The collimator is one of the high-heat-flux components used to avoid a series of vacuum and thermal problems. In this paper, the heat load distribution throughout the collimator is first calculated through experimental data, and a transient thermodynamic simulation analysis of the original model is carried out. The error of the pipe outlet temperature between the simulated and experimental values is 1.632%, indicating that the simulation result is reliable. Second, the model is optimized to improve the heat transfer performance of the collimator, including the contact mode between the pipe and the flange, the pipe material and the addition of a twisted tape in the pipe. It is concluded that the convective heat transfer coefficient of the optimized model is increased by 15.381% and the maximum wall temperature is reduced by 16.415%; thus, the heat transfer capacity of the optimized model is effectively improved. Third, to adapt the long-pulse steady-state operation of the experimental advanced superconducting Tokamak (EAST) in the future, steady-state simulations of the original and optimized collimators are carried out. The results show that the maximum temperature of the optimized model is reduced by 37.864% compared with that of the original model. The optimized model was changed as little as possible to obtain a better heat exchange structure on the premise of ensuring the consumption of the same mass flow rate of water so that the collimator can adapt to operational environments with higher heat fluxes and long pulses in the future. These research methods also provide a reference for the future design of components under high-energy and long-pulse operational conditions

A Virtual Optical Encryption System Based on Polarization Optics

Kang-Ning Wu,Yu-Xiang Zheng,Dong-Xu Zhang,Yu-Hui Tao,Rong-Jun Zhang,Liang-Yao Chen 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.60 No.8

In this paper, an encryption system is proposed based on polarization optics. A modulated image at reference plane was obtained by using a virtual magneto-optical modulator, and the field from the reference plane interfered with that from the information plane having the original image to form an encrypted image. This system not only has the advantages of multiple freedom degrees, high security strength and data manipulation in high-dimension as the traditional optical information processing system but also possesses the advantages of data processing flexibility in the computer information process. Numerical experiments prove that images encrypted by using this system have a high level of security, so it is hard for attackers to analyze the original images.

Influence of Poor Light on Photosynthesis and Carbohydrates in Peach Plants

Yuncong Yao,Yun Kong,Ning Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Batch sequential adaptive designs for global optimization

Xiao Yao,Ning Jianhui,Xiong Zikang,Qin Hong 한국통계학회 2022 Journal of the Korean Statistical Society Vol.51 No.3

Efficient global optimization (EGO) is one of the most popular sequential adaptive design (SAD) methods for expensive black-box optimization problems. A well-recognized weakness of the original EGO in complex computer experiments is that it is serial, and hence the modern parallel computing techniques cannot be utilized to speed up the running of simulator experiments. For those multiple points EGO methods, the heavy computation and points clustering are the obstacles. In this work, a novel batch SAD method, named “Accelerated EGO”, is forwarded by using a refined sampling/importance resampling (SIR) method to search the points with large expected improvement (EI) values. The computation burden of the new method is much lighter, and the points clustering is also avoided. The efficiency of the proposed batch SAD is validated by nine classic test functions with dimension from 2 to 12. The empirical results show that the proposed algorithm indeed can parallelize original EGO, and gain much improvement compared against the other parallel EGO algorithm especially under high-dimensional case. Additionally, the new method is applied to the hyper-parameter tuning of support vector machine (SVM) and XGBoost models in machine learning. Accelerated EGO obtains comparable cross validation accuracy with other methods and the CPU time can be reduced a lot due to the parallel computation and sampling method.

An Aligned Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Scaffold Fixed with Fibronectin to Enhance the Attachment and Growth of Human Endothelial Progenitor Cells

Chien-Ning Hsu,Ya-Ting Lin,Yu-Hsu Chen,Tsung-Yu Tseng,Hsing-Fen Tsai,Shinn-Gwo Hong,Chao-Ling Yao 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.3

Repair and regeneration of vascular tissue is a crucial current research focus in the fields of biomedical engineering and regenerative medicine. Numerous studies revealed that cells are required to grow on an appropriate extracellular matrix to maintain or enhance functionality. In the present study, various surface modification methods were evaluated to fix fibronectin on the surface of a bio-based and aligned poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film for vascular tissue engineering. After chemical modification, the properties of the fibronectin-fixed PHBV films were examined and compared with the original films, including -NH2 group expression, contact angle, mechanical properties, and fibronectin binding amount. Then, cytotoxicity and biocompatibility were measured by culture with L929 cells and endothelial progenitor cells (EPCs) of the fibronectinfixed PHBV films. In addition, cell morphology, cell growth kinetics, acetylated low-density lipoprotein uptake ability, lectin binding ability and specific gene expressions of cultured EPCs on fibronectin-fixed PHBV films were also analyzed. Taken together, our data demonstrated that the surface of the aligned PHBV films could be successfully modified to immobilize fibronectin. Importantly, EPCs cultured on the fibronectin-fixed PHBV films showed excellent cell biocompatibility, a rapid proliferation rate, an aligned growth direction and correct cell functions. We believed that fibronectin-fixed PHBV films can serve as a potential scaffold for vascular tissue engineering.