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Kim, Y.-M.,Cheon, C.-K.,Park, K.-H.,Park, S. W.,Kim, G.-H.,Yoo, H.-W.,Lee, K.-A.,Ko, J. M. INSTITUTE FOR CLINICAL SCIENCE 2016 Annals of clinical and laboratory science Vol.46 No.4
<P>Short-chain acyl-CoA dehydrogenase (SCAD) catalyzes the first step in mitochondrial short-chain beta-oxidation, and its deficiency is caused by mutations in the ACADS. We sought to investigate the spectrum ACADS mutations and associated clinical manifestations in Korean patients with SCAD deficiency. The study included ten patients with SCAD deficiency from 8 unrelated families as diagnosed by biochemical profile and mutation analyses. Clinical features, biochemical data, growth, and neurodevelopmental state were reviewed retrospectively. Eight patients were found during newborn screening, and two were diagnosed by family screening. During follow-up ranging from 2 months to 4.5 years, no hypoglycemic event was noted, and the development and growth of the patients were normal, except in two siblings. One exhibited hypotonia and gross motor delay, while one girl showed cyclic vomiting until the age of two years. We identified seven different mutations of ACADS. Of these, p. E344G was the most frequent mutation with an allele frequency of 50%, followed by p. P55L with 18.8%. p. G108D and four novel mutations were identified: p. L93I, p. E228K, p. P377L, and p. R386H. Korean patients with SCAD deficiency showed heterogenous clinical features and ACADS genotype. Our data contributes to a better understanding of the distinct molecular genetic characteristics and clinical manifestations of SCAD deficiency.</P>
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유형별 완전혼합사료 급여가 반추위내의 발효성상 및 영양소 소화율에 미치는 영향
이덕윤,고종렬,최낙진,이상석,송재용,이세영,박성호,성하균,하종규 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5
This study was conducted to examine effects of feeding dry TMR(DTMR), wet TMR(WTMR) and fermented TMR(FTMR) on rumen fermentation, enzyme activity and digestibility in the total tract of sheep. Three rumen cannulated sheep were used in a 3 × 3 latin square design. The present results showed that ?, NH_(3)-N, total and individual VFA, A/P ratio and enzymes (CMCase, Xylanase and Protease) activity in the rumen were higher in WTMR and FTMR compared with DTMR. In addition, dry matter, organic matter, crude protein, ether extract, NDF and ADF digestibility in the total tract were also higher in WTMR and FTMR compared with DTMR. Therefore, the present results showed that WTMR and FTMr are better than DTMR for rumen fermentation and nutrients digestibility.
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손시환,고영두,김두환,박구부,이정규,이철영,신철교,정희식,곽석준,박명구,천민성,백철승 한국축산학회 2000 한국축산학회지 Vol.42 No.1
The Korean Native Stripped Cattle known as Chickso have distinctive black hair belts all over the body to varying extents on a Hanwoo(Korean Cattle)-like yellowish brown background. These Battle are remaining only in a limited area of this country and are known to yield a flavorful meat somewhat distinct from that of Hanwoo, but their genetic lineage has not been identified. We have carried out karyotyping of these cattle firm the lymphocyte culture. Blood samples were collected from 20 of male and female cattle that had been bred at Poongjeon Farm located in Kosong, Kyongnam, and were subjected to chromosomal morphology and G- and C-banding analysis. Chickso, like Hanwoo, had 58 autosomes and X and Y sex chromosomes which were morphologically very similar to those of the latter. All the 58 autosomes revealed almost a zero value of centromeric index, suggesting that they are acrocentric; sex chromosomes X and Y were submetacentric and metacentric, respectively. Following G-banding, the light bands appeared near the centromeric site in all the autosomes whereas the specific dark bands were consistently visible in each homologous chromosome. Overall, the G-banding pattern was nearly identical between the Chickso and Hanwoo. C-bands representing the heterochromatin were present at or near the centromere in all the autosomes, whereas in sex chromosomes, they were found distributed on variable sites. The proportion of constitutive heterochromatin ranged 20∼30%. These patterns were not significantly different between the two subspecies. All of these cytogenetic results suggest that the distinctive traits of Chickso did not arise from a cytogenetic variation from Hanwoo, i. e. the former is simply a subpopulation of the latter. As such, we propose that selection and propagation of the Chickso based on economic traits may be advantageous to the domestic beef industry.
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Stemmed DNA nanostructure for the selective delivery of therapeutics
Jin, H.,Kim, M. G.,Ko, S. B.,Kim, D. H.,Lee, B. J.,Macgregor, Jr., R. B.,Shim, G.,Oh, Y. K. The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.16
<P>DNA has emerged as a biocompatible biomaterial that may be considered for various applications. Here, we report tumor cell-specific aptamer-modified DNA nanostructures for the specific recognition and delivery of therapeutic chemicals to cancer cells. Protein tyrosine kinase (PTK)7-specific DNA aptamer sequences were linked to 15 consecutive guanines. The resulting aptamer-modified product, AptG15, self-assembled into a Y-shaped structure. The presence of a G-quadruplex at AptG15 was confirmed by circular dichroism and Raman spectroscopy. The utility of AptG15 as a nanocarrier of therapeutics was tested by loading the photosensitizer, methylene blue (MB), to the G-quadruplex as a model drug. The generated MB-loaded AptG15 (MB/AptG15) showed specific and enhanced uptake to CCRF-CEM cells, which overexpress PTK7, compared with Ramos cells, which lack PTK7, or CCRF-CEM cells treated with a PTK7-specific siRNA. The therapeutic activity of MB/AptG15 was tested by triggering its photodynamic effects. Upon 660 nm light irradiation, MB/AptG15 showed greater reactive oxygen species generation and anticancer activity in PTK7-overexpressing cells compared to cells treated with MB alone, those treated with AptG15, and other comparison groups. AptG15 stemmed DNA nanostructures have significant potential for the cell-type-specific delivery of therapeutics, and possibly for the molecular imaging of target cells.</P>
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중년남성의 지각된 건강상태, 가족응집력 및 건강증진행위에 관한 연구
김지선,노원영,박새별,유동민,이가은,이주애,전유라,조세영,차지영,고지현 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50
health promotion behavior of middle aged men and the relationship among the above factors. Methods: Subjects of the research were 325 middle aged men in Korea. The questionnaires were composed of general characteristics, Health Self Rating Scale, FACES-Ⅲ : Family Adaptability & Cohesion Evaluation Scale Ⅲ, Health Promoting Lifestyle Profile. Data were statistically analyzed by t-test, ANOVA, Scheffe test and Pearson’s correlation. Results: Middle aged men whose perceived economic status are low show lower perceived health status than moderate and high (F=3.364, p=.010). Health promotion behavior in the age 40-44 shows lower level than 60-64 (F=2.984, p=.019). Middle aged men who have Economically dependent adult in the first child show higher level in health promotion behavior than who have Middle & High school student in the first child (F=2.468, p=.045). In the middle aged men, perceived health status and family cohesion show positive correlation (r=.341, p<.01). Perceived health status and health promotion behavior show positive correlation (r=.500, p<.01). Family cohesion and health promotion behavior show posotive correlation (r=.564, p<.01). Conclusions: We analyzed influencing factor and a relationship between perceived health status, family cohesion and health promotion behavior. Accordingly, we can think of applying nursing interventions to the middle aged men considering the general characteristics. Also we can think of applying nursing interventions and educations to help them evaluate their perceived health status and include the support of family members for the health promotion behavior.
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Jung, M-J,Rho, J-K,Kim, Y-M,Jung, J E,Jin, Y B,Ko, Y-G,Lee, J-S,Lee, S-J,Lee, J C,Park, M-J Macmillan Publishers Limited 2013 Oncogene Vol.32 No.2
The hypothesis of cancer stem cells has been proposed to explain the therapeutic failure in a variety of cancers including lung cancers. Previously, we demonstrated acquisition of epithelial–mesenchymal transition, a feature highly reminiscent of cancer stem-like cells, in gefitinib-resistant A549 cells (A549/GR). Here, we show that A549/GR cells contain a high proportion of CXCR4+ cells that are responsible for having high potential of self-renewal activity in vitro and tumorigenicity in vivo. A549/GR cells exhibited strong sphere-forming activity and high CXCR4 expression and SDF-1α secretion compared with parent cells. Pharmacological inhibition (AMD3100) and/or siRNA transfection targeting CXCR4 significantly suppressed sphere-forming activity in A549 and A549/GR cells, and in various non-small cell lung cancer (NSCLC) cell lines. A549/GR cells showed enhanced Akt, mTOR and STAT3 (Y705) phosphorylation. Pharmacological inhibition of phosphatidyl inositol 3-kinase or transfection with wild-type PTEN suppressed phosphorylation of Akt, mTOR and STAT3 (Y705), sphere formation, and CXCR4 expression in A549/GR cells, whereas mutant PTEN enhanced these events. Inhibition of STAT3 by WP1066 or siSTAT3 significantly suppressed the sphere formation, but not CXCR4 expression, indicating that STAT3 is a downstream effector of CXCR4-mediated signaling. FACS-sorted CXCR4+ A549/GR cells formed many large spheres, had self-renewal capacity, demonstrated radiation resistance in vitro and exhibited stronger tumorigenic potential in vivo than CXCR4− cells. Lentiviral-transduction of CXCR4 enhanced sphere formation and tumorigenicity in H460 and A549 cells, whereas introduction of siCXCR4 suppressed these activities in A549/GR cells. Our data indicate that CXCR4+ NSCLC cells are strong candidates for tumorigenic stem-like cancer cells that maintain stemness through a CXCR4-medated STAT3 pathway and provide a potential therapeutic target for eliminating these malignant cells in NSCLC.
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Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5
<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>
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