Soliton Transmission Experiment Employing Normal Dispersion Fiber with Large Dispersion

Jian Jun Yu,Ke Jian Guan,Bo Jun Yang 한국통신학회 1997 OPTOELECTRONICS & COMMUNICATIONS CONFERENCE Vol.2 No.1

CircularRNA_104670 plays a critical role in intervertebral disc degeneration by functioning as a ceRNA

Jian Son,Hong-Li Wang,Ke-Han Song,Zhi-Wen Ding,Hai-Lian Wang,Xiao-Sheng Ma,Fei-Zhou Lu,Xin-Lei Xia,Ying-Wei Wang,Fei-Zou,Jian-Yuan Jiang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

This study was carried out to explore the roles of circular RNAs (circRNAs) in nucleus pulposus (NP) tissues in intervertebral disc degeneration (IDD). Differentially expressed circRNAs in IDD and normal NP tissues were identified based on the results of microarray analysis. Bioinformatics techniques were employed to predict the direct interactions of selected circRNAs, microRNAs (miR), and mRNAs. CircRNA_104670 was selected as the target circRNA due to its large multiplier expression in IDD tissues. After luciferase reporter and EGFP/RFP reporter assays, we confirmed that circRNA_104670 directly bound to miR-17-3p, while MMP-2 was the direct target of miR-17-3p. The receiver-operating characteristic (ROC) curve showed that circRNA_104670 and miR-17-3p had good diagnostic significance for IDD (AUC circRNA_104670 = 0.96; AUC miRNA-17-3p = 0.91). A significant correlation was detected between the Pfirrmann grade and expression of circRNA_104670 (r = 0.63; p = 0.00) and miR-17-3p (r = −0.62; p = 0.00). Flow-cytometric analysis and the MTT assay showed that interfering with circRNA_104670 using small interfering RNA (siRNA) inhibited NP cell apoptosis (p < 0.01), and this inhibition was reduced by interfering with miR-17-3p. Interfering with circRNA_104670 suppressed MMP-2 expression and increased extracellular matrix (ECM) formation, which were also reduced by interfering with miR-17-3p. Finally, an MRI evaluation showed that circRNA_104670 inhibition mice had a lower IDD grade compared with control mice (p < 0.01), whereas circRNA_104670 and miRNA-17-3p inhibition mice had a higher IDD grade compared with circRNA_104670 inhibition mice (p < 0.05). CircRNA_104670 is highly expressed in the NP tissues of IDD and acts as a ceRNA during NP degradation.

Tools Setting of 5 Axis Ultra-Precision Single point Diamond Lathe without OTS

Ke-Tian Li,Xin Chen,Xin-Du Chen,Qiang Liu,Xiang-bo Ouyang,Hong-Jian Xia,Jian-Qun Liu 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9(2)

Ab Initio and Experimental Studies on Dibenzothiazyl-Disulfide

Jian, Fang-Fang,Zhang, Ke-Jie,Zhao, Pu-Su,Zheng, Jian Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.7

Ab initio calculations of the structure, atomic charges and natural bond orbital (NBO) have been performed at HF/6-311G** and B3LYP/6-311G** levels for the title compound of dibenzothiazyl-disulfide. The calculated results show that the two nitrogen atoms have the biggest negative charges and they are the potential sites to react with the metallic ions, which make the title compound become a di-dentate ligand. Vibrational frequencies of the title compound have been obtained and compared with the experimental value and the comparison indicates that B3LYP/6-311G** level is better than HF/6-311G** level to predict the vibrational frequencies for the system studied here. For the title compound, electronic absorption spectra calculated by time?ependent density functional theory (TD-DFT) are more accurate than Hartree-Focksingle-excitation CI (CI-Singles) method. NBO analyses show that the electronic transitions are mainly derived from the contribution of bands $\pi\rightarrow\pi^{*}$. Thermodynamic calculated results show that the formation of the title compound from 2-mercaptobenzothiazole is a spontaneous process at room temperature with the change of free Gibbs being negative value.

Effects of pyrite bioleaching solution of Acidithiobacillus ferrooxidans on viability, differentiation and mineralization potentials of rat osteoblasts

Jian Zhou,Hong Yu Li,Ke-Ming Chen,De Juan Zhi,Qin-Jian Xie,Cory J. Xian 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.12

Iron pyrite, an important component of traditional Chinese medicine, has a poor solubility, bioavailability, and patient compliance due to a high dose required and associated side effects, all of which have limited its clinical applications and experimental studies on its action mechanisms in improving fracture healing. This study investigated Acidithiobacillus ferrooxidans (A.f)-bioleaching of two kinds of pyrites and examined bioactivities of the derived solutions in viability and osteogenic differentiation in rat calvarial osteoblasts. A.f bioleaching improved element contents (Fe, Mn, Zn, Cu, and Se) in the derived solutions and the solutions concentration-dependently affected osteoblast viability and differentiation. While the solutions had no effects at low concentrations and inhibited the osteoblast alkaline phosphatase (ALP) activity at high concentrations, they improved ALP activity at their optimal concentrations. The improved osteoblast differentiation and osteogenic function at optimal concentrations were also revealed by levels of ALP cytochemical staining, calcium deposition, numbers and areas of mineralized nodules formed, mRNA and protein expression levels of osteogenesis-related genes (osteocalcin, Bmp-2, Runx-2, and IGF-1), and Runx-2 nuclear translocation. Data from this study will be useful in offering new strategies for improving pyrite bioavailability and providing a mechanistic explanation for the beneficial effects of pyrite in improving bone healing.

New Method of Designing Controller for Uncertain Diff erential-Diff erence Systems and Application to Time-Delay Operational Amplifi er System

Ke Jian,Chung-Cheng Chen,Yen-Ting Chen,Gui-hong Lin 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.6

This study combines the feedback linearization method and our recent researches of Chen electrical unifying approach to propose a global tracking controller design for nonlinear uncertain diff erential-diff erence systems with time delay. One example, which cannot be solved by the fi rst paper on the almost disturbance decoupling problem, is presented in this study to show the essential points that the tracking performance is easily solved by the proposed approach. The study applies Chen electrical unifying approach to take the place of using the disorganized virtual ground technique and the Kirchhoff ’s law for those traditional approaches. In order to show its signifi cant practicability, the study has fi rstly designed an easy-to-implement adder and a time-delay circuit, and an unstable time-delay operational amplifi er control system based on the Chen electrical unifying approach, and then has proposed a stable controller.

Ab Initio and Experimental Studies on Dibenzothiazyl-Disulfide

Fang Fang Jian*,Ke Jie Zhang,Pu Su Zhao,Jian Zheng 대한화학회 2006 Bulletin of the Korean Chemical Society Vol.27 No.7

Light-Chain Cardiac Amyloidosis: Cardiac Magnetic Resonance for Assessing Response to Chemotherapy

Guo Yubo,Li Xiao,Gao Yajuan,Shen Kaini,Lin Lu,Wang Jian,Cao Jian,Zhang Zhuoli,Wan Ke,Zhou Xi Yang,Chen Yucheng,Zhang Long Jiang,Li Jian,Wang Yining 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.5

Objective: Cardiac magnetic resonance (CMR) is a diagnostic tool that provides precise and reproducible information about cardiac structure, function, and tissue characterization, aiding in the monitoring of chemotherapy response in patients with lightchain cardiac amyloidosis (AL-CA). This study aimed to evaluate the feasibility of CMR in monitoring responses to chemotherapy in patients with AL-CA. Materials and Methods: In this prospective study, we enrolled 111 patients with AL-CA (50.5% male; median age, 54 [interquartile range, 49–63] years). Patients underwent longitudinal monitoring using biomarkers and CMR imaging. At followup after chemotherapy, patients were categorized into superior and inferior response groups based on their hematological and cardiac laboratory responses to chemotherapy. Changes in CMR findings across therapies and differences between response groups were analyzed. Results: Following chemotherapy (before vs. after), there were significant increases in myocardial T2 (43.6 ± 3.5 ms vs. 44.6 ± 4.1 ms; P = 0.008), recovery in right ventricular (RV) longitudinal strain (median of -9.6% vs. -11.7%; P = 0.031), and decrease in RV extracellular volume fraction (ECV) (median of 53.9% vs. 51.6%; P = 0.048). These changes were more pronounced in the superior-response group. Patients with superior cardiac laboratory response showed significantly greater reductions in RV ECV (-2.9% [interquartile range, -8.7%–1.1%] vs. 1.7% [-5.5%–7.1%]; P = 0.017) and left ventricular ECV (-2.0% [-6.0%–1.3%] vs. 2.0% [-3.0%–5.0%]; P = 0.01) compared with those with inferior response. Conclusion: Cardiac amyloid deposition can regress following chemotherapy in patients with AL-CA, particularly showing more prominent regression, possibly earlier, in the RV. CMR emerges as an effective tool for monitoring associated tissue characteristics and ventricular functional recovery in patients with AL-CA undergoing chemotherapy, thereby supporting its utility in treatment response assessment.

A Promising Injectable Scaffold: The Biocompatibility and Effect on Osteogenic Differentiation of Mesenchymal Stem Cells

Ke Ding,Yu Long Zhang,Zhong Yang,Jian Zhong Xu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1

Chitosan/β-glycerophosphate/collagen (C/GP/Co)is a promising injectable scaffold in the bone tissue engineering. In this study, we prepared this scaffold and evaluated its biocompatibility and effects on the osteogenic differentiation of mesenchymal stem cells (MSCs). After fabrication, the C/GP/Co hydrogel was examined in a scanning electron microscope (SEM) and showed a porous microstructure. Its biocompatibility was assessed by cell morphology and cell viability assays. Cell morphological observations were performed by fluorescent microscope in 2D cultivation and by laser confocal scanning microscope (LCSM) in 3D cultivation, respectively. Cell viability in 2D and that in 3D cultivation were both evaluated by the Cell Counting Kit-8 (CCK-8) assay. Its effect on osteogenic differentiation of MSCs in vitro was clarified by alkaline phosphatase (ALP) activity, Alizarin Red staining, and real-time polymerase chain reaction (Real-time PCR). An additional experiment of the ectopic bone formation in nude mice was conducted to investigate its effects on osteogenic differentiation of MSCs after subcutaneous injection. The results proved that C/GP/Co hydrogel exhibited good biocompatibility and enhanced the in vitro osteogenic differentiation of MSCs. In the experiment of ectopic bone formation, this hydrogel demonstrated its capability of supporting neovascularization and differentiation of MSCs toward osteogenic lineage. Therefore, C/GP/Co hydrogel scaffold holds a great promise for the bone tissue engineering applications.

Enhanced Visible-Light Driven Photocatalytic Performances Over LaFeO3/NiO Modified Porous g-C3N4 Nanosheets

Ke Xu,Xiaosheng Yang,Dagao Sun,Xin Yang,Yumei Zhou,Wei Li,Qindan Yang,Xianjiong Yang,Rong Li,Jian Feng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.02

LaFeO3/NiO modified g-C3N4 nanosheets (L-N/CNS) were synthesized by a two-step method. HRTEM results showed that an intimate contact between LaFeO3, NiO and N-CNS was successfully established. Ninety percent of phenol was degraded within 120 min, and the hydrogen evolution rate of 171.2 μmol h -1 g -1 was obtained over the L-N/CNS heterojunctions under the visible-light irradiation. It was higher than that of g-C3N4 nanosheets, NiO modified g-C3N4 and LaFeO3/g-C3N4. The ·O2 - radicals acted the crucial role in the photocatalytic degradation reaction. EIS, PL and time-resolved fluorescence spectra demonstrated that L-N/CNS possessed the highest charge separation efficiency. The intimate contact between LaFeO3, NiO and g-C3N4 nanosheets promoted the separation and transfer of photo-induced electron–hole pairs and consequently prolonged the exciton lifetime, and implied more photo-induced electrons could be probably involved in the photocatalytic reactions on the surface of photocatalysts. Thus, the visible-light driven photocatalytic performances of L-N/CNS were effective. This work provided a feasible method to design and construct heterostructures for the exploitation of solar energy.