Manipulations of glucose/lipid metabolism and gut microbiota of resistant starch encapsulated Ganoderma lucidum spores in T2DM rats

Yumei Jiang,Na Zhang,Yawen Zhou,Zhongkai Zhou,Yu Bai,Padraig Strappe,Chris Blanchard 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.5

Our team previously demonstrated that Ganodermalucidum spores (GLS) and resistant starch (RS) hadhypoglycemic effects separately on type 2 diabetic mellitus(T2DM) rats. This work was to explore the effects ofadministering encapsulated GLS within RS (referred to asEGLS) in the T2DM rats, which were induced by streptozotocin(STZ). The EGLS was orally administered torats for 28 days. The parameters of glycometabolismand lipometabolism were evaluated, and fecal microbiotacomposition was investigated. The results showed thatEGLS significantly enhanced glycometabolism and lipometabolismparameters in T2DM rats, which might beassociate with the enhancement of the glucose and lipidmetabolism, insulin secretion, and glycogen synthesis andreduced lipogenesis. Furthermore, the intervention ofEGLS also reduced the Proteobacteria community andimproved dysfunctional gut microbiota. This study indicatedEGLS may be a potential candidate for dietaryintervention to modulate diabetes.

Output Feedback Adaptive Control for Stochastic Non-strict-feedback System with Dead-zone

Yumei Sun,Bingwei Mao,Hongxia Liu,Shaowei Zhou 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.10

This paper focuses on the problem of adaptive neural network (NN) control for a class of nonlinear stochastic non-strict feedback system with dead-zone input. A novel adaptive NN output feedback control approach is first proposed for stochastic non-strict feedback nonlinear systems. In order to solve the problem of dead-zone input, a linear decomposition method is proposed. On the basis of the state observer, an output feedback adaptive NN controller is designed by a backstepping approach. It is shown that the proposed controller guarantees that all the signals of the closed-loop systems are semi-globally uniformly bounded in probability. Simulation results further illustrate the effectiveness of the proposed approach.

Experiments and FEM Simulations of Fracture Behaviors for ADC12 Aluminum Alloy Under Impact Load

Yumei Hu,Yue Xiao,Xiaoqing Jin,Haoran Zheng,Yinge Zhou,Jinhua Shao 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.6

Using the combination of experiment and simulation, the fracture behavior of the brittle metal named ADC12aluminum alloy was studied. Five typical experiments were carried out on this material, with responding datacollected on different stress states and dynamic strain rates. Fractographs revealed that the morphologies offractured specimen under several rates showed different results, indicating that the fracture was predominantly abrittle one in nature. Simulations of the fracture processes of those specimens were conducted by Finite ElementMethod, whilst consistency was observed between simulations and experiments. In simulation, the Johnson-Cook model was chosen to describe the damage development and to predict the failure using parametersdetermined from those experimental data. Subsequently, an ADC12 engine mount bracket crashing simulation wasconducted and the results indicated good agreement with the experiments. The accordance showed that ourresearch can provide an accurate description for the deforming and fracture processes of the studied alloy.

Involvement of Orai1 in tunicamycin-induced endothelial dysfunction

Hui Yang,Yumei Xue,Sujuan Kuang,Mengzhen Zhang,Jinghui Chen,Lin Liu,Zhixin Shan,Qiuxiong Lin,Xiaohong Li,Min Yang,Hui Zhou,Fang Rao,Chunyu Deng 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.2

Endoplasmic reticulum (ER) stress is mediated by disturbance of Ca2+ homeostasis. The store-operated calcium (SOC) channel is the primary Ca2+ channel in non-excitable cells, but its participation in agent-induced ER stress is not clear. In this study, the effects of tunicamycin on Ca2+ influx in human umbilical vein endothelial cells (HUVECs) were observed with the fluorescent probe Fluo-4 AM. The effect of tunicamycin on the expression of the unfolded protein response (UPR)-related proteins BiP and CHOP was assayed by western blotting with or without inhibition of Orai1. Tunicamycin induced endothelial dysfunction by activating ER stress. Orai1 expression and the influx of extracellular Ca2+ in HUVECs were both upregulated during ER stress. The SOC channel inhibitor SKF96365 reversed tunicamycin-induced endothelial cell dysfunction by inhibiting ER stress. Regulation of tunicamycin-induced ER stress by Orai1 indicates that modification of Orai1 activity may have therapeutic value for conditions with ER stress-induced endothelial dysfunction.

Genome-wide association analysis for yield-related traits at the R6 stage in a Chinese soybean mini core collection

Li Xiangnan,Zhou Yang,Bu Yuanpeng,Wang Xinfang,Zhang Yumei,Guo Na,Zhao Jinming,Xing Han 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background Soybean (Glycine max (L.) Merr.) is an economically important crop for vegetable oil and protein production, and yield is a critical trait for grain/vegetable uses of soybean. However, our knowledge of the genes controlling the vegetable soybean yield remains limited. Objective To better understand the genetic basis of the vegetable soybean yield. Methods The 100-pod fresh weight (PFW), 100-seed fresh weight (SFW), kernel percent (KP) and moisture content of fresh seeds (MCFS) at the R6 stage are four yield-related traits for vegetable soybean. We investigated a soybean mini core collection composed of 224 germplasm accessions for four yield-related traits in two consecutive years. Based on 1514 single nucleotide polymorphisms (SNPs), genome-wide association studies (GWAS) were conducted using a mixed linear model (MLM). Results Extensive phenotypic variation existed in the soybean mini core collection and signifcant positive correlations were shown among most of traits. A total of 16 SNP markers for PFW, SFW, KP and MCFS were detected in all environments via GWAS. Nine SNP markers were repeatedly identifed in two environments. Among these markers, eight were located in or near regions where yield-related QTLs have been reported in previous studies, and one was a novel genetic locus identifed in this study. In addition, we conducted candidate gene analysis to the large-efect SNP markers, a total of twelve genes were proposed as potential candidate genes of soybean yield at the R6 stage. Conclusion These results will be benefcial for understanding the genetic basis of soybean yield at the R6 stage and facilitating the pyramiding of favourable alleles for future high-yield breeding by marker-assisted selection in vegetable soybean.

Involvement of Orai1 in tunicamycin-induced endothelial dysfunction

Yang, Hui,Xue, Yumei,Kuang, Sujuan,Zhang, Mengzhen,Chen, Jinghui,Liu, Lin,Shan, Zhixin,Lin, Qiuxiong,Li, Xiaohong,Yang, Min,Zhou, Hui,Rao, Fang,Deng, Chunyu The Korean Society of Pharmacology 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.2

Endoplasmic reticulum (ER) stress is mediated by disturbance of $Ca^{2+}$ homeostasis. The store-operated calcium (SOC) channel is the primary $Ca^{2+}$ channel in non-excitable cells, but its participation in agent-induced ER stress is not clear. In this study, the effects of tunicamycin on $Ca^{2+}$ influx in human umbilical vein endothelial cells (HUVECs) were observed with the fluorescent probe Fluo-4 AM. The effect of tunicamycin on the expression of the unfolded protein response (UPR)-related proteins BiP and CHOP was assayed by western blotting with or without inhibition of Orai1. Tunicamycin induced endothelial dysfunction by activating ER stress. Orai1 expression and the influx of extracellular $Ca^{2+}$ in HUVECs were both upregulated during ER stress. The SOC channel inhibitor SKF96365 reversed tunicamycin-induced endothelial cell dysfunction by inhibiting ER stress. Regulation of tunicamycin-induced ER stress by Orai1 indicates that modification of Orai1 activity may have therapeutic value for conditions with ER stress-induced endothelial dysfunction.

Damping and transformation behaviors of Ti50(Pd50−xCrx) shape memory alloys with x ranging from 4.0 to 5.0

Deqing Xue,Ruihao Yuan,Dezhen Xue,Yumei Zhou,Guojun Zhang,Xiangdong Ding,Jun Sun 한국물리학회 2018 Current Applied Physics Vol.18 No.7

The damping and transformation behaviors of Ti50(Pd50−xCrx) shape memory alloys with x ranging from 4.0 to 5.0 are systematically investigated. The damping capacity (Q−1) at the martensitic transformation is found to be inversely proportional to the square root of frequency, i.e., Q−1∝ω−0.5. A relaxation peak or shoulder is observed slightly below the martensitic transformation damping peak for compositions within the compositional crossover region (4.5 ⩽x⩽ 4.8). Furthermore, the damping capacity at the martensitic transformation is smaller within the compositional crossover region (4.5 ⩽x⩽ 4.8), compared with that of compositions at both sides (x = 4.0 and x = 5.0). These observations can be ascribed to the hysteretic motion of interfaces between different phases near the compositional crossover region.

Microstructure and Mechanical Properties of Mg–2.0Gd–1.2Y–1.0Zn–0.2Zr Alloy

Jianan Liu,Xiaoru Zhang,Wenxue Lv,Yumei Zhou,Daqing Fang,Xiangdong Ding,Jun Sun 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

The microstructural evolution of Mg–2.0Gd–1.2Y–1.0Zn–0.2Zr (GWZK) alloy during homogenization and subsequentaging heat-treatment and the tensile properties of the peak-aged sample at various temperatures have been investigated. Thephase transformation during homogenization, static precipitation during aging, and deformation mechanisms at 250 °C aresystematically analyzed through XRD, TEM and EBSD measurements. The peak-aged GWZK alloy has good combinationof strength and ductility at elevated temperatures owing to its high thermal stability by the LPSO phases distributed alongthe grain boundaries. The change (19 MPa) in the yield strength of peak-aged samples between RT and 250 °C is mainlyrelated to easy gliding of dislocations on the non-basal planes due to the decrease in critical resolved shear stress of slip. Two strengthening models are applied to quantitatively describe the efect of {11 −2 0}훼precipitate plates on Orowanstrengthening corresponding to RT and 250 °C. Ignoring changes in solid solution strengthening and grain boundary strengthening of the peak-aged sample caused by increasing temperature, the calculated reduction value (16.5 MPa) in macroscopicyield strength from RT (based on the basal<a>slip mode) to 250 °C (based on the pyramidal<c+a>slip mode) is in goodagreement with the measured reduction value (19 MPa).

Enhanced Visible-Light Driven Photocatalytic Performances Over LaFeO3/NiO Modified Porous g-C3N4 Nanosheets

Ke Xu,Xiaosheng Yang,Dagao Sun,Xin Yang,Yumei Zhou,Wei Li,Qindan Yang,Xianjiong Yang,Rong Li,Jian Feng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.02

LaFeO3/NiO modified g-C3N4 nanosheets (L-N/CNS) were synthesized by a two-step method. HRTEM results showed that an intimate contact between LaFeO3, NiO and N-CNS was successfully established. Ninety percent of phenol was degraded within 120 min, and the hydrogen evolution rate of 171.2 μmol h -1 g -1 was obtained over the L-N/CNS heterojunctions under the visible-light irradiation. It was higher than that of g-C3N4 nanosheets, NiO modified g-C3N4 and LaFeO3/g-C3N4. The ·O2 - radicals acted the crucial role in the photocatalytic degradation reaction. EIS, PL and time-resolved fluorescence spectra demonstrated that L-N/CNS possessed the highest charge separation efficiency. The intimate contact between LaFeO3, NiO and g-C3N4 nanosheets promoted the separation and transfer of photo-induced electron–hole pairs and consequently prolonged the exciton lifetime, and implied more photo-induced electrons could be probably involved in the photocatalytic reactions on the surface of photocatalysts. Thus, the visible-light driven photocatalytic performances of L-N/CNS were effective. This work provided a feasible method to design and construct heterostructures for the exploitation of solar energy.