A Study on DNA Sequences and Mutation of Integrase Region of Korean-type Bovine Leukemia Virus (BLV) pol Gene

권오식,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

Bovine leukemia virus (BLV) is a causative agent for lymphoma disease in cattle including cows worldwide. BLV shares similar virion structure and characteristics with other retroviruses. The pol gene of the BLV genome produced reverse transcriptase (RT) and integrase (IN) for important roles for BLV genome integration into host cell chromosomes that is known to be coded in the 3' side of the BLV pol gene (one third portion). In this study, we have sequenced 978 bp in the 3'side of the BLV pol gene from BLV 10C3 in order to determine the BLV IN region of it. And we compared it to the nucleotide sequences of an Australian BLV isolate. As a result, nucleotide sequences of the IN region of the Korean-type BLV pol gene were mutated at a rate of 3.7%. We can confirm that the typical mutations are such as Arg (AGG) → Lys(AAG), Thr (ACG) → Met (ATG), Ile (ATT) → Val (GTT), Asn (ACC) → His (CAC), Phe (TTT) → Leu (TTG) and Asn (ACC) → Asp (GAC). From the analysis of the sequencing data, we were able to determine the zinc-finger-like "HHCC" motif in the amino terminus of BLV IN, that was H-X_(3)-H-X_(25)-C-X_(2)-C. It was also found the DD35E motif in the IN catalytic domain as D-X_(56)-D-X_(35)-E. It fits very well to the consensus sequences of retroviral IN as well as HHCC motif.

개에 있어서 耳根部의 Lidocaine 水針에 의한 全身 無痛覺의 誘發

권현주,유건주,이상은,이정연,송근호,김명철,정성목,박성준,김덕환 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Twelve clinically healthy mongrel dogs were used to clarify the possibility of general analgesia induction by lidocaine aquapuncture to auricular root area in the present study. The experimental animals were devided into control (6 heads) and experimental groups (6 heads), respectively. Physiological salines (3 ml/kg) were injected into both auricular root areas in control group. Lidocaine hydrochlorides (3 ml/kg) were injected into both auricular root areas in experimental group. Superficial pain, the changes of vital signs and blood leukocytes, and PCV were examined on pre, 5 min., 15 min., 30 min, and 60 min. after injection in control and experimental groups, respectively. Analgesia was not detected at all in control group, however, all experimental animals were recumbent and analgesia was generally detected in the auricles, noses, dorsal parts, ventral areas, limbs and tails from 5 to 30 minutes after injection with lidocaine hydrochloride into auricular root areas in experimental group. As for the change of vital signs, body temperature showed decreasing tendency from 5 minutes after lidocaine injection in experimental group. Heart rates and respiration rates were increased on 5 minutes after lidocaine injection and then they showed decreasing tendency in experimental group. No significant difference were found among groups. As for the changes of hematological findings, the numbers of blood leukocytes were increased till 15 minutes after lidocaine injection and then they showed decreasing tendency. PCV values were increased on 5 minutes after lidocaine injection, and then they showed decreasing tendency. However, no significant differences were observed among groups. Considering the above findings, it was thought that general analgesia was possible by aquapuncture with lidocaine hydrochloride into auricular root areas and lidocaine aquapuncture didn’t affect so much to the changes of vital signs and blood.

무대미술의 관례성 : 맨션(Mansion)에서 '임의의 궁전'에 이르기까지 des mansions au palais a` volonte´

권현정 한국 프랑스어문교육학회 2003 프랑스어문교육 Vol.15 No.-

Comme tout art, le the´a^tre a un mode de fonctionnement spe´cifique et ne peut se contenter de copier la re´alite´, me^me s'il exploite des donne´es (personnages, fiction, espace, temps, langage) qui appartiennent au monde de l'expe´rience. Mais ce monde de l'expe´rience ne peut se transporter tel quel sur la sce`ne; l'espace sce´nique, du fait me^me de ses dimensions, ne peut donner qu'un aperc¸u, qu'un concentre´ des lieux e´voque´s par les fictions. De la` naissent des conventions qui sont des modes de fonctionnement fonde´s sur l'accord du spectateur. La de´coration-type est une des conventions essentielles qui couvre la longue pe´riode qui va du Moyen a^ge au the´a^tre classique du 17e`me sie`cle. Que ce soit sur la sce`ne simultane´e ou sur la sce`ne unique, cette convention repose sur l'espace neutre qui fait nai^tre une autre convention, celle du jeu de l'acteur qui demande a` son tour une grande complicite´/re´ceptivite´ de la part du spectateur. Les acteurs du Moyen Age utilisent l'espace sce´nique place´ devant les mansions de fac¸on tout a` fait arbitraire, comme une sorte de lieu de´veloppe´ en prolongement des diffe´rentes mansions. Ne´anmoins ces conventions-la` sont admises sans difficulte´s par le public. Mais au 17e`me sie`cle, les conventions vont devenir progressivement a` la fois plus pre´cises et plus codifie´es du fait de l'intervention des doctes. Ces conventions provoquent des discussions, du moins des remaniements de la part des cre´ateurs car elles entrent en conflit avec un autre grand principe, celui de la vraisemblance. D'autre part, les conventions sont changeantes et de´termine´es par la mentalite´ des spectateurs. De la sce`ne simultane´e a` la sce`ne unique, des mansions au palais a` volonte´ en passant par la pluralite´ des lieux ou le lieu composite des tragi-come´dies ou des pastorales on n'assiste pas seulement a` l'e´volution de l'esthe´tique de la sce´nographie conventionnelle. Il s'agit aussi d'une forme de mentalite´ : pour les gens du Moyen Age, tout signe exte´rieur vaut une re´alite´ palpable auquel on croit sans mettre en doute sa perception imme´diate. On pourrait parler alors d'une convention spontane´e. Pour les Franc¸ais des anne´es 1630-50, la sce´nographie simultane´e est une manifestation de l'esprit baroque, fait de fantaisie, de liberte´ et d'imagination : le spectateur se prome`ne en imagination en divers lieux situe´s pourtant, de fac¸on totalement invraisemblable, co^te a` co^te sur la sce`ne exigue¨ de l'Ho^tel de Bourgogne. Sans doute a` la sortie de la pe´riode trouble´e de la Fronde, dans les anne´es 1660, il n'est pas e´tonnant qu'on e´volue d'un gou^t anarchique vers l' n'est pas e´tonnant qu'on e´volue d'un gou^t anarchique vers l'unite´ de lieu qui n'est d'autre qu'une maifestation du de´sir de rationaliser, d'organiser aussi bien l'espace fictif du plateau que le monde lui-me^me. Pourtant, ce de´sir de se rationaliser a commence´ a` se faire sentir me^me en ces temps de liberte´. Un signe caracte´ristique de ce changement de mentalite´ est donne´ par l'usage des tapisseries : ces rideaux de tissu descendus des cintres masquent les compartiments dont on ne se sert pas a` tel ou tel moment de l'action sce´nique ; on tend au vraisemblance par l'utilisation de tapisseries qui ont pour but de re´duire l'arbitraire. On imagine par la` le combat, qui va durer plusieurs ge´ne´rations, entre des conventions de moins en moins bien accepte´es et un besoin de vraisemblance qui finira par s'imposer a` la sce´nographie du 19e`me sie`cle sous le nom de re´alisme sce´nique puis de naturalisme. Pourtant, au moment ou` la sce`ne d'illusion se perfectionne jusqu'au de´tail avec le naturalisme, la convention renai^t et se renouvelle : 'la convention consciente' veut de´voiler le fonctionnement propre du the´a^tre ; elle devient le signe de reconnaissance de la the´a^tralite´. Par tous ces e´le´ments de´libe´re´ment propose´s comme arbitraires, la sce´nographie fait comprendre au spectateur qu'il est au the´a^tre, qu'il assiste a` un jeu dont on ne cherche plus a` masquer le me´canisme. Ainsi nai^t une nouvelle vision du monde et du the´a^tre : plus c'est faux, plus c'est vrai ; en multipliant les signes visibles de l'artifice du the´a^tre, on essaye de s'approcher de la ve´rite´ des choses...

국내 혈액질환 환자에서 침습성 아스페르길루스증의 구제치료로서 Voriconazole의 효과와 안전성

권재철,김시현,최수미,최재기,이동건,박선희,최정현,유진홍,신완식 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Invasive aspergillosis (IA) is associated with significant morbidity and mortality in patients with hematologic malignancies. We investigated the efficacy and safety of voriconazole (VCZ) when used as salvage therapy for IA in Korean adults with hematologic malignancies who had not responded to prior antifungal therapy. Materials and Methods: We retrospectively reviewed data, collected from January 2007 to October 2008, from patients with proven or probable cases of IA. All were probable IA cases, except for one proven case. All cases were refractory or intolerant to antifungal therapy prior to administration of VCZ. Efficacy and safety were assessed in patients treated with VCZ for more than 3 days and for more than one dose, respectively. A favorable response [complete (CR) or partial (PR)] was defined by significant improvement of all clinical symptoms, signs, and radiologic abnormalities. Results: Fifty patients who met the inclusion criteria were enrolled. There were 27 male and 23 female patients with mean age of 44.4 years (range, 15-65 years). Underlying diseases were acute leukemia (35 cases), chronic myelogenous leukemia (4 cases), myelodysplastic syndrome (3 cases), lymphoma (3 cases) and other hematologic diseases (5 cases). Twenty-two patients had received chemotherapy and 13 patients had undergone hematopoietic stem cell transplantation. The lung was the main infection site (94%) followed by the sinus (6%). Amphotericin B deoxycholate alone was the most frequent previous antifungal therapy. The mean duration of antifungal therapy prior to VCZ therapy was 13.9±8.8 days (2-44 days). The median duration of VCZ therapy was 19 days (interquartile range, 49 days). Sixteen patients (32.0%) showed favorable responses (CR:PR=8:8) at the end of VCZ therapy. The numbers of patients with stable disease, progression and death were, 6 (12%), 6 (12%) and 22 (44%) respectively. Most of those with unfavorable responses had relapsed underlying malignancies or refractory graft versus host diseases. Twelve patients developed drug-related adverse events but only one patient stopped VCZ treatment prematurely. Conclusions: VCZ demonstrated an acceptable level of toxicity in patients with hematologic malignancies but further studies are required to prove its efficacy as salvage therapy.

OMR 답안지 판독을 위한 영상의 전처리 방법

권갑현,서정상 동양대학교 2000 동양대학교 논문집 Vol.6 No.1

Recently, in every class of schools and evaluation institutes they are checking the OMR exam sheets through a card reader for exclusive use. Not only because it is very expensive but also because there is few domestic made card reader, it's true that we usually depend on imported products. On the other hand, as the image processing techniques have been improved largely, a digital camera which is image-inputting device is spreading widely. This study suggests a preprocessing method that can put OMR exam sheet in by using a digital camera and read it by using a PC. After we emphasize the contrast of the image that was put into a PC, make it binary, and clear the isolated noises in the binarized image, and then we can make marts on the card be interpreted exactly.

흰 쥐에서 Olanzapine의 장간 재순환을 설명하기 위한 약동학적 모델

백현문,채정우,권광일 충남대학교 약학대학 의약품개발연구소 2014 藥學論文集 Vol.29 No.-

Abstract - Olanzapine is an atypical antipsychotic drug that is widely used for the treatment of schizophrenia and acute bipolar disorder. It had been reported that olanzapine was shown enterohepatic recirculation in rat and human. But pharmacokinetic model for enterohepatic recirculation of olanzapine had not been developed yet. In this study, we developed pharmacokinetic model for enterohepatic recirculation of olanzapine. 30mg/kg of olanzapine was administered to rats (n=7) by oral and plasma concentration of olanzapine was determined by HPLC-MS/MS(API2000). Noncompartmental analysis was done by WinNonlin and pharamcokinetic model was developed by NONMEM ver 7.2. Enterohepatic recirculation model was developed with two compartment including gall bladder compartment with mtime option and evaluation was done by visual predictive check(VPC) and bootstrap. As a result of VPC and bootstrap, our model was robust and parameter values were reliable. In conclusion, pharmacokinetic model for enterohepatic recirculation of olanzapine was successfully developed and evaluated. The model was appropriate to predict the plasma concentration of olanzapine in rat and may be useful to develop human model in further study.

유치 수복물에 따른 주변 법랑질의 탈회 저항성 : QLF를 이용한 연구

권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

본 연구에서는 유치 수복물의 불소 방출 여부에 따른 주변 법랑질의 탈회 저항성과 재광화 효과를 살펴보고자 하였다. 건전 유전치 48개를 16개씩 임의로 세 군으로 나누어, FiltekTM Z250(1군), F2000(2군), KetacTM N100(3군)을 사용하여 제조사의 지시에 따라 충전하였다. 3일 동안 인공 우식 병소를 유발한 후, 14일 동안 인공 타액에 담가 재광화를 유발하였다. Quantitative light-induced fluorescence(QLF)를 이용하여 실험 단계와 시간에 따른 무기질 소실량(ΔQ)의 변화를 관찰한 결과는 다음과 같다. 1. 탈회가 일어난 인공 우식 병소의 ΔQ 값은 3군, 2군, 1군 순으로 작게 나타났고, 이 중 3군은 1군에 비해 유의한 탈회 저항성을 보였다. 2. 1군, 2군, 3군 모두 재광화 1일 후부터 초기 인공 우식 병소의 ΔQ 값과 비교 시 유의한 차를 보였으며, 관찰 기간 동안 지속적인 증가 양상을 나타내었다. 3. 재광화 속도를 의미하는 Δ(ΔQ)/일 값은 각 군에서 처음 1일 동안 유의성 있게 크게 나타났으며, 그 이후 급속히 감소하였다. 4. 수복 재료에 따른 재광화 정도는 각 군 간에 유의한 차이가 없었다. The objective of this in vitro study was to detect and monitor demineralization and remineralization of primary teeth according to restorative materials using quantitative light-induced fluorescence (QLF). A single bur hole was drilled on the each sound forty eight primary anterior teeth, and the specimens were divided into three groups. The cavity was restored with FiltekTM Z250(Group 1), F2000(Group 2), KetacTM N100(Group 3) following the manufacturer's instructions. The teeth were subjected to the demineralizing buffer for 3 days, and then subjected to a remineralizing buffer for 14 days. The change of mineral loss(ΔQ) according to the stages was evaluated by QLF and the following results were obtained: 1. When demineralization was done, ΔQ was increased as follows. : Group 1 (-110.79 ± 27.77) < Group 2 (-104.84 ± 28.95) < Group 3 (-90.16 ± 21.87) : Resistance to demineralization was statistically significant in Group 3. 2. There was a statistically significant increase in ΔQ of all groups since 1st day of remineralization 3. The rate of remineralization, Δ(ΔQ)/day, showed significant high value in each group on the 1st day then decreased rapidly. 4. There was no statistically significant difference in the degree of remineralization among restorative materials.

Streptococcus mutans 로 부터 분리된 mutan의 화학적 특성

권현정,한만덕 순천향대학교 기초과학연구소 2011 순천향자연과학연구 논문집 Vol.17 No.1

Streptococcus mutans produces water-soluble and water-insoluble α-glucan from sucrose by multiple glucosyltransferase (GTFs). Mutan, primarily water-insoluble α-glucan plays an important role in the development of dental plaque. The study was conducted to investigate the chemical properties of mutan purified from crude extracellular polysaccharide of S. mutans. In batch culture of S. mutans, the percent yield(%) of crude extracellular polysaccharide was calculated as 0.026%. Total carbohydrate and protein contents of crude polysaccharide showed 67.3 and 24.3%, respectively. The contents of hexosamine from extracellular polysaccharide extract were found to be 2.4%. Sugar composition analysis showed that mutan obtained from purification of crude polysaccharide is mainly composed of glucose with a slight amount of galactose. In an infrared absorption spectrum of the mutan, the absorption peak in the range of 700 ∼ 900 cm^(-1) indicated that the mutan contained α-1,3-glucan. The ^(13)C NMR spectrum analysis shows that mutan has a high similarity with the α-1,3 linked glucan.

영상 처리 기법을 이용한 OMR 답안지 판독

권갑현,서정상 동양대학교 1999 동양대학교 논문집 Vol.5 No.1

In this paper, a method for scoring OMR exam sheets using image processing techniques is suggested. In many schools, the special-purpose OMR card reader is used to score OMR exam sheets, but it is very expensive and foreign-made as well. Jn this method, a PC and a digital camera are used, so it is relatively low-priced. In addition, the intermediate images produced in this procedure can be stored easily in the computer storage media. Therefore, the necessary store space can be significantly reduced, and the specific card image can be easily retrieved.

사회교육 무용 프로그램이 정신건강에 미치는 영향에 대한 연구

권의준,윤현정 목원대학교 자연과학연구소 2000 自然科學 硏究論文集 Vol.9 No.1

본 연구는 구청 문화원에서 주관하는 사회교육 프로그램 중 중년 여성들이 무용 프로그램에 참가후 신체적 정신적 건강 측면에서 효과를 검증하고자 하였다. 본 연구의 목적을 달성하기 위해서 서울특별시 소재 3개 구청의 문화원과 경기도 고양시 관내 3개의 문화원의 프로그램 참가자 138명이 본 연구에 참여 하였으며 이들이 정신건강차원에서 여러 발생 요인들을 공변량 분석을 통해 분석한 결과 프로그램 참여자가 비참여자에 비해 정신적 건강에 대한 치료효과가 있음을 입증하고 있다.