대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

The Influence of Verbal Aggression on Job Involvement and Turnover Intention: The Moderating Effect of Self-esteem

Jun-Hwa Song,Nam-Gyum Lee,Il-Young Hwang,Soo-Wook Lee 한국유통과학회 2015 Asian Journal of Business Environment (AJBE) Vol.5 No.4

Purpose – Research shows that increased workload and psychological pressure produces tensions in organizations. Such tensions can increase employee aggressive workplace behavior. This study attempted to discover the relationship among verbal aggression, job involvement, and turnover intention. Research design, data, and methodology – A survey of administrative workers in colleges was done between May 20 and May 26, 2015. Result – The findings were as follows. First, verbal aggression directly increased employee turnover intention. Second, stress recognition also created turnover intention among employees, which was detected in the interviews conducted after the survey. Third, both the survey and the interviews showed that stress recognition did not have a significant effect on job involvement. Fourth, both the findings and the interviews after the survey showed that stress increased turnover intention. Last, the finding on the role of self-esteem showed that self-esteem had a positive moderating effect on the relationship between the influence of verbal aggression and stress recognition. Conclusion – Having strong talent at work should lower turnover intention and turnover rates and develop the organization continuously.

Genetic Polymorphism of Epoxide Hydrolase and GSTM1 in Lung Cancer Susceptibility of Korean Population

Jun Hwa Hwang,Kyu Sik Kim,Yu Il Kim,Eun Joung Kim,Kyung Hwa Park,Gye Jung Cho,Jin Young Ju,Sung Chul Lim,김영철,박경옥,박종태,Sung-Ja Ahn 대한암학회 2003 Cancer Research and Treatment Vol.35 No.6

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성공적인 당뇨병 치료를 위한 유전자 전달 기술을 이용한 췌장 소도 세포 이식

황진욱(Hwang Jun Wook),황용화(Hwang Yong Hwa),이동윤(Lee Dong Yun) 한양대학교 고령사회연구원 2011 한양고령사회논집 Vol.2 No.2

본 총평 논문은 고령화 사회에 따른 당뇨병에 대해 인지하고 당뇨병 치료 방식을 논의하고자 한다. 고령화가 되면서 65세 이상 노인 사망원인 4위가 당뇨병이다. 그러므로 노인성 질환으로의 당뇨병 치료가 필요하다. 현재 당뇨병을 치료하기 위한 대표적 치료방법은 인슐린 단백질 의약품 주사이다. 하지만 지속적인 인슐린 주사는 환자에게의 불편감을 줄 뿐만 아니라 저혈당을 유발할수 있다. 가장 이상적인 치료방법은 혈중 포도당 수치에 맞춰서 적절한 양의 인슐린 단백질을 지속적으로 분비할 수 있는 췌도(pancreatic islet)를 이식하는 것이다. 하지만, 췌도세포 이식 시 기증자(donor)의 절대적 부족과 췌도세포를 분리, 배양, 이식 과정 동안에 저산소 현상(hypoxia)에 의한 췌도세포의 손상 등이 발생한다. 따라서 이식 준비 및 이식 후 체내에서 췌도세포를 건강한 상태로 유지시킬 수 있는 다양한 방법들이 개발될 필요가 있다. 본 총평에서는 현재 Heme oxygenase-1 (HO-1) 및 Vascular endothelial growth factor (VEGF) 등의 유전자 전달기술을 이용한 췌도세포를 보호(protection)할 수 있는 방법들에 대해 요약을 하였다. This review will be discussed about cognizance of diabetes and diabetes therapy according to aging society. Becoming aging society, diabetes is the 4th leading cause of death of aged 65 and older. Thus, treatment of diabetes is needed. Typical treatment is an insulin injection. However, insulin injections can cause hypoglycemia, and patient feel uncomfortable. The ideal way is pancreatic islet transplantation that can simultaneously control glucose. However this method has several problems such as lack of contributors, transplanted islets are rapidly eliminated from the body because of rare islet engraftment, hypoxia, and islet graft rejection. Among them, hypoxia-indued islet death is main issue. Thus, it is necessary to develop the remedy for successful pancreatic islet transplantation without hypoxia induction. This review summarized that gene delivery such as Heme oxygenase-1 (HO-1), Vascular endothelial growth factor (VEGF) could protect the transplanted islets from hypoxia.

비대칭 난류 채널 유동에서 벽 부착 구조

황준혁(Jun Hyuk Hwang),황현규(Hyeon Gyu Hwang),이재화(Jae Hwa Lee),황진율(Jinyul Hwang) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.6

비대칭 난류 채널 유동의 직접 수치 모사를 통해 벽 부착 구조의 특성을 조사하였다. 벽면 속도가 증가하면서 최대 평균 속도의 위치가 정지한 벽면으로 이동한 비대칭 층(layer)을 형성하였다. 양 벽면의 레이놀즈 수는 증가하지만, 정지한 벽면에서 평균속도의 대수 영역 (log region)은 짧아지고 움직이는 벽면에서는 확대되었다. 난류 강도는 정지한 벽면에서 감소하고 움직이는 벽면에서 증가하는 것을 보여주었다. 또한, 스펙트럼 분석은 kz-1 영역이 정지한 벽면에서 짧아지고, 움직이는 벽면에서 확장되는 것을 보여준다. 이는 큰 구조 (large-scale motion)의 변화가 벽 부착 구조의 차이를 초래하여 비대칭 채널의 난류 특성에 큰 영향을 주는 것을 알 수 있다. The DNSs of turbulent asymmetric channel flows are performed to examine the characteristics of wall-attached structures. As the moving wall velocity increases, the position of the maximum mean velocity are shifted to the stationary wall, creating asymmetric layer. Although the Reynolds numbers on both walls increase, the logarithmic layer is shortened on the stationary wall, and extended on the moving wall. The turbulent intensities shows that the turbulent activity decreases on the stationary wall and increases on the moving wall. The spectral analysis also shows the shortened/extended kz-1 region clearly, reminiscent the suppression/growth of wall attached structures. The variation of large-scale motions between the stationary and moving walls influence on the difference of wall attached structure and contribute to the turbulent characteristics of an asymmetric wall-bounded flows.

Inhibition of SNARE-driven neuroexocytosis by plant extracts

Jung, Chang Hwa,Yang, Yoo Soo,Kim, Jun-Seob,Shin, Yeon-Kyun,Hwang, Jae Sung,Son, Eui Dong,Lee, Hong Hwa,Chung, Koo Min,Oh, Jung Mi,Lee, Jong Hwa,Kweon, Dae-Hyuk Springer-Verlag 2009 Biotechnology letters. Vol.31 No.3

소세포 폐암의 간전이에 의하여 발생한 전격간기능상실 1예

황영태 ( Young Tae Hwang ),신정우 ( Jung Woo Shin ),이준호 ( Jun Ho Lee ),황대성 ( Dae Sung Hwang ),음준범 ( Jun Bum Eum ),최혜정 ( Hye Jeong Choi ),박능화 ( Neung Hwa Park ) 대한간학회 2007 Clinical and Molecular Hepatology(대한간학회지) Vol.13 No.4

UV조사에 의해 유도된 피부섬유아세포의 세포사에 미치는 Sea Buckthorn (Hippophae rhamnoides L.) 열매추출물의 영향

황인식(In Sik Hwang),고은경(Eun Kyoung Koh),김지은(Ji Eun Kim),이영주(Young Ju Lee),곽문화(Moon Hwa Kwak),고준(Jun Go),성지은(Ji Eun Sung),송성화(Sung Hwa Song),황대연(Dae Youn Hwang) 한국생명과학회 2014 생명과학회지 Vol.24 No.5

Sea buckthorn (Hippophae rhamnoides L.)은 flavonoid, carotenoids, steroids, vitamin, tannins, oleic acid 등의 생물학적으로 활성이 높은 성분을 풍부하게 함유하고 있어 오랫동안 피부질환(skin diseases), 위궤양(gastric ulcers), 천식(asthma), 폐질환 등에 사용되어 왔다. 본 연구에서는 아시아와 유럽의 고지대에서 야생으로 자라는 Sea buckthorn의 열매추출물(SBFE)이 UV조사 후 유도되는 세포사와 연관성을 규명하기 위하여, 세포사와 관련된 다양한 인자의 변화를 관찰하였다. 그 결과, 세포사를 유발할 수 있는 UV조사농도는 400 mJ로 설정하였고, 세포생존율은 UV + LoC, UV + MeC, UV + HiC 처리군에서는 유의적으로 증가하였다. 또한 FACS와 DAPI염색의 결과에서 PI로 염색된 세포수는 UV + vehicle 처리군에서 급격히 증가한 이후 UV + SBFE 처리군에서 유의적으로 감소하였고, Annexin V가 염색된 세포 수는 고농도의 SFBE을 처리한 UV + HiC 처리군에서 유의적으로 감소하였다. 그러나 PI와 Annexin V가 동시에 염색된 세포의 수는 UV + LoC 처리군에서 오히려 증가하였고 나머지 그룹에서는 유의적인 변화가 관찰되지 않았다. 한편, 세포사와 관련된 단백질의 변화에 대한 연구에서 caspase-3는 pro 형태와 active 형태 모두에서 유의적인 차이를 나타내지 않았으며, Bax와 Bcl-2 모두 UV 무처리군에 비하여 UV + Vehicle 처리군에서 감소하였으나 Bax의 발현양은 UV + SBFE 처리군에서 농도의존적으로 증가한 반면, Bcl-2의 발현양은 UV + SBFE 처리군에서 추가적으로 더욱 감소하였다. 따라서 이상의 결과들은 SBFE가 UV조사에 의해 유도된 세포사를 억제하거나 회복시키는 기능을 가지고 있음을 제시하고 있어 향후 UV조사에 의해 유발되는 다양한 상해에 대한 치료제로서의 가능성을 제시하고 있다. Sea buckthorn (Hippophae rhamnoides L.) is a well-known and rich source of biologically active compounds, such as flavonoids, carotenoids, steroids, vitamins, tannins, and oleic acid. The effects of sea buckthorn fruit extract (SBFE) on ultraviolet (UV)-induced cell death was investigated in SK-MEL-2 cells cotreated with UV and a low concentration (LoC), medium concentration (MeC), or high concentration (HiC) of SBFE. Cell viability gradually decreased in accordance with an increase in the UV dose. The cell viability of the UV+SBFE cotreated cells increased significantly compared to that of UV+vehicle-treated cells during the application of an appropriate UV radiation dose (400 mJ). In addition, the number of 4’,6-diamidino-2-phenylindole (DAPI), propidium iodine (PI)-, and annexin V-stained apoptotic cells was higher in the UV+vehicle-treated cells than in the UV untreated cells. The decrease of apoptotic cell numbers varied in each treated group, but it was most significant in the SBFE-treated group. The number of PI-stained cells dramatically decreased in accordance with the concentration of SBFE, and the maximum decrease was detected in the UV+HiC-treated group. In addition, Bax expression increased and Bcl-2 expression decreased in the SBFE-treated group compared with the UV-only treated group. The level of caspase-3 remained constant in all the groups. These results suggest that SBFE may contribute to a recovery from UV-induced cell death through the regulation of apoptotic protein expression and that it may have potential therapeutic utility in ameliorating UV-induced skin ageing.

HMGB1 modulation in pancreatic islets using a cell-permeable A-box fragment

Hwang, Yong Hwa,Kim, Min Jun,Lee, Yong-Kyu,Lee, Minhyung,Lee, Dong Yun Elsevier 2017 Journal of controlled release Vol.246 No.-

<P><B>Abstract</B></P> <P>Although pancreatic islet implantation is an attractive strategy for curing diabetes mellitus, implanted cells are immunologically eliminated due to early islet graft loss. One of main issues in early islet graft loss is the secretion of high-mobility group-box-1 (HMGB1) protein from the damaged islet cells, which is known as a cytokine-like factor. Therefore, regulating the activity of HMGB1 protein offers an alternative strategy for improving outcomes of islet cell therapy. To this end, we first demonstrated that HMGB1 protein could be bound to its A-box fragment (HMGB1 A-box) with higher binding affinity, resembling anti-HMGB1 antibody. To be used as a pharmaceutical protein <I>ex vivo</I>, TAT-labeled HMGB1 A-box-His<SUB>6</SUB> (TAT-HMGB1A) was structurally modified for cellular membrane penetration. TAT-HMGB1A significantly reduced secretion of endogenous HMGB1 protein through interaction in the cytosol without any damage to the viability or functionality of the islets. When TAT-HMGB1A-treated islets were implanted into diabetic nude mice, they completely cured diabetes, as evidenced by stable blood glucose level. TAT-HMGB1A treatment could also reduce the marginal islet mass needed to cure diabetes. Furthermore, TAT-HMGB1A positively protected xenotransplanted islets from xenogeneic immune reactions. Collectively, cell-penetrable TAT-HMGB1A could be used to modulate HMGB1 activity to increase successful outcomes of <I>ex vivo</I> pancreatic islet cell therapy.</P> <P><B>Graphical abstract</B></P> <P> <I>Ex vivo</I> cell-permeable HMGB1 A-box fragment delivery into pancreatic islet could offer enhanced viability of islets from immune reactions, thereby supporting successful pancreatic islet transplantation to cure diabetes mellitus.</P> <P>[DISPLAY OMISSION]</P>

A Facile Synthesis of SAPO-34 Molecular Sieves with Microwave Irradiation in Wide Reaction Conditions

Jun, Jong-Won,Lee, Ji-Sun,Seok, Hwi-Young,Chang, Jong-San,Hwang, Jin-Soo,Jhung, Sung-Hwa Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.6

Various reaction conditions uding temperature, time and type and concentration of templates have been changed in order to facilely synthesize, especially with microwave (MW) heating, SAPO-34 molecular sieves. SAPO-34 molecular sieve can be synthesized rapidly with microwave irradiation from a gel containing tetraethylammonium hydroxide (TEAOH) as a template. However, other several templating molecules lead to SAPO-5 molecular sieve under microwave irradiation even though SAPO-34 is obtained by conventional electric synthesis from the same reactant gels. Moreover, SAPO-34 can be obtained more easily by increasing the TEAOH or silica concentration or by increasing the reaction temperature. SAPO-34 can be obtained within 5 min in a selected condition (high temperature of 210 $^{\circ}C$) with microwave heating, which may lead to a continuous production of the important material. SAPO-34 synthesized by microwave irradiation is homogeneous and small in size and shows acidity and a stable performance in the dehydration of methanol and 2-butanol to olefins, suggesting potential applications in acid catalysis.