Lactobacillus acidophilus 88과 Lactobacillus bulgaricus IFO 13953간의 세포융합주의 특성에 관한 연구

조영배,김혜정,김성구,백형석,전홍기 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

유산균의 균주개량방법의 일환으로 protoplast fusion 기법과 electrofusion법을 이용하여 protease 활성, lipase 활성, 내열성, 내산성 등이 우수한 L. bulgaricus 와 bacteriocin을 생산하는 L. acidophilus를 융합시켜 얻은 융합주들의 생리학적 성질을 검토하였다. 산 생성능, 내열성, 내산성, protease, lipase 활성 등은 L. bulgaricus가 L. acidophilus 보다 우수하였다. L. bulgaricus는 lactose와 sorbose를 이용하였으나 maltose와 sorbitol을 이용하지 못하는 반면, L. acidophilus는 maltose를 이용하고 lactose와 sorbose를 이용하지 못하였다. 융합주 가운데서는 3, 6, 7, 8, 10번이 모균주의 발효능 특성을 함께 지님으로서 재조합체임을 확인 할 수 있었으며, sorbitol의 경우 모균주에서는 발효능이 전혀 나타나지 않았음에도 불구하고 융합주 4, 7번이 발효능을 나타내어 융합과정에서 새로운 형질을 획득하기도 한다는 사실을 알 수 있었다. Lactase 활성은 모균주 모두 높은 β-galactosidase 활성을 보였으나, phospho-β-galactosidase 활성은 거의 없었으며 융합주들도 다소 차이는 있었지만 모균주와 유사한 효소 활성을 나타내었다. 발효에 있어서 key enzyme으로 작용하는 protease, lipase 등의 효소 활성도 모균주의 활성 보다 우수한 융합주도 존재하였다. Aninterspecific fusant was made from the protoplasts of two strains of lactobacillus genus (streptomycin and lincomycin resistant L. bulgaricus and kanamycin resistant L. acidophilus 88). The functional properties of the fusant were examined by determining bacteriocin productivity, acid producing activity, ability of carbohydrates fermentation and three important enzyme activities. The recombinant strain revealed bacteriocin productivity. Acid production and β galactosidase, phospho-β-galactosidase, lipase and protease activity of L. bulgaricus were better than those of L. acidophilus 88. Among fusants, β-galactosidase activity of two strains were better than that of the parent strains but phospho-β-galactosidase activity remarkably lower. One fusant revealed the improved proteolysis compared to that of the parent strains- Lipase activity of L. bulgaricus was better than that of L. acidophilus but another fusant exhibited the highest lipase activity.

원발성 후복막 점액낭샘암종 1예

지준호,이화정,박승찬,박정철,최은정,서혜진,이원식,이정림,배병조,손경락,이경희 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Primary retroperitoneal mucinous cystadenocarcinoma is a very rare malignancy, and little is known concerning its Pathogenesis, optimal treatment, and prognosis. A 29-year-o1d pregnant woman (21 weeks) Presented with abdominal discomfort CA 19-9, CA 125, and CEA were normal Abdominal CT scanning revealed a 19x15x13 cm retropentoneal tumor Exploratory laparotomy and tumor excision were performed. Mucinous retropentoneal implants were removed as completely as possible Histologically, the tumor showed focal areas of capsular invasion, but free resection margins The uterus and both ovaries were normal in appearance No adjuvant therapy was pursued. Six months later, Peritoneal and bilateral ovarian metastases were discovered. Hence, we report the details of this case of primary retroperitoneal mucinous Cystadeno-carcinoma and present a review of the literature.

췌장 선방세포암 1예

이화정,지준호,박승찬,박정철,최은정,서혜진,이원식,이정림,배병조,손경락,이경희 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Acinar cell carcinoma is a rare tumor that represents 1~2% of al1 pancreatic cancers. Clinical and radiologic findings are inconclusive in this disease Acinar cell carcinoma is characterized by rapid progression and early metastasis, which lead to its poor prognosis. A 41-year-o1d man was admitted to our hospital for abdominal pain. Abdominal computed tomography (CT) and positron emission tomography-computed tomography (PET-CT) showed a splenic mass, which was being invaded by a pancreatic tail mass and which had increased ^(18)F-fluorodeoxyglucose (FDG) uptake Primary radical distal pancreatectomy and splenectomy were performed. Pathologic findings revealed an acinar cell carcinoma of the Pancreas The patient underwent a total gastrectomy three months later because of gastric recurrence Four months later, multiple hepatic metastases were discovered, and the patient underwent a left hepatectomy During treatment with capecitabine, there was no evidence of tumor progression for 14 months. We report a case of metastatic pancreatic acinar cell carcinoma, which did not progress for an extended period while the patient was being treated with capecitabme.

쑥 및 인삼 급여가 한국재래산양육에 있어서 지방산 조성 및 TBA가에 미치는 효과

한규호,유신아,김민준,조혜연,이치호 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

본 연구는 쑥, 인삼 및 쑥과 인삼 병용첨가가 흑염소육 중의 지질, 지방산 조성 및 TBA가에 어떤 영향을 미치는지를 알아보기 위하여 실시하였다. 그 결과 쑥, 인삼 및 쑥과 인삼 병용첨가 사육시 총지질을 유의하게(p<0.01) 저하시키는 작용이 있었으며 흑염소육의 지방산 조성을 크게 변화시켜 linoleic acid 함량을 낮추면서, palmitic acid 함량을 증가시켰다. 또한, 흑염소육의 TBA가를 유의하게(p<0.05) 낮추는 것을 볼 때 흑염소육에 다량 함유된 불포화 지방산의 산패를 억제하는 것으로 사료되었다. 이들 결과는 흑염소육의 노린내를 제거해 육질을 개선시키는 필요한 기초자료가 될 것으로 기대된다. This study was performed to investigate the effects of mugwort and ginseng on the fatty acid composition and TBA value of meats in Korean Native Goat. A Total of 12 Korean Native Goats were equally into four groups; Control, Mugwort(4%), Ginseng(1%), Mugwort & Ginseng(4%+1%) groups were supplemented to the basal diet for 40days. These Korean Native Goats with the liveweight of average 9.9kg were slaughtered to obtain samples from loin. Meat samples stored at -20℃ for 7days were analyzed quantitatively to obtain the changes of fatty acid composition and TBA value in total lipid. The results obtained were summarized as follows : 1. Total lipid contents were significantly(P<0.01) low in Ginseng and Mugwort & Ginseng groups, compared with the Control group. 2. Composition of fatty acid in total lipid of loin were categorized with 6 different fatty acids. Palmitic acid content from loin was highest among saturated fatty acid followed by stearic acid and myristic acid, and oleic acid content was highest among unsaturated fatty acids followed by linoleic acid and palmitoleic acid. Palmitic acid levels decreased remarkably in Mugwort, Ginseng and Mugwort & Ginseng groups, compared with the Control. Linoleic acid levels also decreased in Mugwort, Ginseng and Mugwort & Ginseng groups. The ratio of the compositions of unsaturated and saturated fatty acids in total lipids increased remakably in Mugwort, Gineng and Mugwort & Ginseng groups compared with the Control group. 3. TBARS(thiobabituric acid related substance) of mugwort, ginseng or mugwort & ginseng supplementation to the diet decreased significantly(p<0.05) in loin. These results indicate that mugwort or ginseng supplementation in Korean Native Goat may be effective, to increase the stability of meat or decrease total lipid and unsaturated fatty acids.

Forecast of Net Photosynthetic Rates using FvCB Model by Different Positions of Cucumber Leaves in Vertically

Won Jun Jo,Ha Seon Sim,Hye Jin Lee,Yu Hyun Moon,Ui Jeong Woo,Soo Bin Jung,Su Ran Ahn,Jung Su Jo,Sung Kyeom Kim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

The photosynthetic rates of leaves depend on the crop characteristics and vertical position of the leaf in terms of leaf age and development stages. The photosynthetic models of crops are an important factor for manipulating the optimum CO₂ concentration and light intensity for crop productivity in a greenhouse. However, few models have been proposed to estimate the net photosynthetic rate according to leaf positions. The purpose of this study was to predict and validate the leaf photosynthetic rate of cucumber (Cucumis sativus L., cv. Goodmorning) by the vertical positions using a photosynthetic model. The leaf photosynthetic rate at the vertical positions was measured under several CO₂ concentrations and light intensity conditions in the three plants. Leaf photosynthetic was analyzed using the FvCB (Farquhar, von Caemmerer, and Berry) model and validated as a multivariable photosynthetic model. As the CO₂ concentration and light intensity increased, the photosynthetic rate of the leaf showed a saturation curve. The CO₂ concentrations and light intensities saturation point were the highest in the upper leaf (at three internodes below the meristems) and lowest in the bottom leaf (at two or three internodes over the substrate area). The maximum carboxylation capacity (μmol·CO₂·m<SUP>-2</SUP>·s<SUP>-1</SUP>) and maximum electron transport rate (μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>) estimated in the FvCB model were high accuracies in regression analysis. As a result of comparing the actual measured photosynthetic rate and the estimated values through the FvCB model, the R² values at the bottom, middle, and upper leaf were 0.89, 0.89, and 0.87, respectively. The root means square errors values at the bottom, middle, and upper leaf were 2.521, 2.958, and 3.200, respectively. The leaf photosynthetic rate for each vertical position estimated from the FvCB model was similar to the actual measured value. Results, the FvCB model could be feasible to establish optimal CO₂ concentration and light intensity to maintain the productivity of cucumber in greenhouse cultivation.

CartoonFaceGAN : StyleGAN2-ADA 기반의 Layer Swap 및 Distillation을 활용한 Cartoon Face Generation 연구

조성운(Seong-Woon Jo),조수한(Su-Han Jo),조혜지(Hye-Ji Jo),전재현(Jae-Hyeon Jun) 한국정보과학회 2022 한국정보과학회 학술발표논문집 Vol.2022 No.12

Analysis of Participant Factors That Affect the Diagnostic Performance of Screening Mammography: A Report of the Alliance for Breast Cancer Screening in Korea

Kim, Young Joong,Lee, Eun Hye,Jun, Jae Kwan,Shin, Dong-Rock,Park, Young Mi,Kim, Hye-Won,Kim, Youme,Kim, Keum Won,Lim, Hyo Soon,Park, Jeong Seon,Kim, Hye Jung,Jo, Hye-Mi unknown 2017 KOREAN JOURNAL OF RADIOLOGY Vol.18 No.4

<P><B>Objective</B></P><P>To analyze participant factors that affect the diagnostic performance of screening mammography.</P><P><B>Materials and Methods</B></P><P>We enrolled 128756 cases from 10 hospitals between 2005 and 2010. We analyzed recall rate, cancer detection rate (CDR) per 1000 examinations, positive predictive value (PPV), sensitivity, specificity, false positive rate (FPR), and interval cancer rate (ICR) per 1000 negative examinations according to participant factors including age, breast density, and number of visit to the same institution, and adjusted for confounding variables.</P><P><B>Results</B></P><P>Increasing age improved recall rates (27.4% in 40's, 17.5% in 50's, 11.1% in 60's, and 8.6% in 70's), CDR (2.7, 3.2, 2.0, and 2.4), PPV (1.0, 1.8, 1.8, and 2.8%), sensitivity (81.3, 88.8, 90.3, and 94.7%), specificity (72.7, 82.7, 89.0, and 91.7%), and FPR (27.3, 17.3, 11.0, and 8.4%) (<I>p</I> < 0.05). Higher breast density impaired recall rates (4.0% in P1, 9.0% in P2, 28.9% in P3, and 27.8% in P4), PPV (3.3, 2.3, 1.2, and 1.3%), specificity (96.1, 91.2, 71.4, and 72.5%), and FPR (3.9, 8.9, 28.6, and 27.6%) (<I>p</I> < 0.001). It also increased CDR (1.3, 2.1, 3.3, and 3.6) and ICR (0.2, 0.3, 0.6, and 1.6) (<I>p</I> < 0.05). Successive visits to the same institution improved recall rates (20.9% for one visit, 10.7% for two visits, 7.7% for more than three visits), PPV (1.6, 2.8, and 2.7%), specificity (79.4, 89.6, and 92.5%), and FPR (20.6, 10.4, and 7.5%) (<I>p</I> < 0.001).</P><P><B>Conclusion</B></P><P>Young age and dense breasts negatively affected diagnostic performance in mammography screening, whereas successive visits to the same institution had a positive effect. Examinee education for successive visits to the same institution would improve the diagnostic performance.</P>

Expression of Cyclomaltodextrinase Gene from Bacillus halodurans C-125 and Characterization of Its Multisubstrate Specificity

Hye-Jeong Kang,Chang-Ku Jeong,Myoung-Uoon Jang,Seung-Ho Choi,Min-Hong Kim,Jun-Bae Ahn,Sang-Hwa Lee,Sook-Ja Jo,Tae-Jip Kim 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

A putative cyclomaltodextrinase (BHCD) gene was found from the genome of Bacillus halodurans C-125, which encodes 578 amino acids with a predicted molecular mass of 67,279 Da. It shares 42-59% of amino acid sequence identity with common cyclomaltodextrinase (CDase)-family enzymes. The corresponding gene was cloned by polymerase chain reaction (PCR) and the dimeric enzyme with C-terminal 6-histidines was successfully overproduced and purified from recombinant Escherichia coli. BHCD showed the highest activity against β-CD at pH 7.0 and 50℃. Due to its versatile hydrolysis and transglycosylation activities, BHCD has been confirmed as a member of CDases. However, BHCD can be distinguished from other typical CDases on the basis of its novel multisubstrate specificity. While typical CDases have over 10 times higher activity on β-CD than starch or pullulan, the CD-hydrolyzing activity of BHCD is only 2.3 times higher than pullulan. In particular, it showed significantly higher activity ratio of maltotriose to acarbose than other common CDase-family enzymes.

Human liver microsome metabolite identification of eutylone, dibutylone, and dimethylone using HPLC-QTOF/MS

Jun Sang Yu,So Young Jo,Hye Hyun Yoo 한국분석과학회 2023 학술대회논문집 Vol.2023 No.5

Development of simultaneous quantitative analysis for amphetamine-type stimulants in human hair by liquid chromatography-quadrupole Orbitrap mass spectrometry

Jun Sang Yu,So Young Jo,Eun Kyu Lee,Jin Young Kim,Jae Chul Cheong,Hye Hyun Yoo 한국분석과학회 2022 학술대회논문집 Vol.2022 No.11