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- 저자 : Jinping Zhao (검색결과 11 건)
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Zhao, Hongying,Zhang, Jun,Shao, Haiyu,Liu, Jianwen,Jin, Mengran,Chen, Jinping,Huang, Yazeng Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.3
Transforming growth factor ${\beta}1$ $(TGF{\beta}1)/Smad4$ signaling plays a pivotal role in maintenance of the dynamic balance between bone formation and resorption. The microRNA miR-155 has been reported to exert a significant role in the differentiation of macrophage and dendritic cells. The goal of this study was to determine whether miR-155 regulates osteoclast differentiation through $TGF{\beta}1/Smad4$ signaling. Here, we present that $TGF{\beta}1$ elevated miR-155 levels during osteoclast differentiation through the stimulation of M-CSF and RANKL. Additionally, we found that silencing Smad4 attenuated the upregulation of miR-155 induced by $TGF{\beta}1$. The results of luciferase reporter experiments and ChIP assays demonstrated that $TGF{\beta}1$ promoted the binding of Smad4 to the miR-155 promoter at a site located in 454 bp from the transcription start site in vivo, further verifying that miR-155 is a transcriptional target of the $TGF{\beta}1/Smad4$ pathway. Subsequently, TRAP staining and qRT-PCR analysis revealed that silencing Smad4 impaired the $TGF{\beta}1$-mediated inhibition on osteoclast differentiation. Finally, we found that miR-155 may target SOCS1 and MITF to suppress osteoclast differentiation. Taken together, we provide the first evidence that $TGF{\beta}1/Smad4$ signaling affects osteoclast differentiation by regulation of miR-155 expression and the use of miR-155 as a potential therapeutic target for osteoclast-related diseases shows great promise.
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Molecular Characterization and Expression Analysis of GhWRI1 in Upland Cotton
Jinping Hua,Yanpeng Zhao,Zhengjie Liu,Xinwang Wang,Yumei Wang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.4
Numerous genes were involved in oil metabolism,transcription factors are the key genes, such as WRINKLED(AtWRI1, At3g54320) in Arabidopsis. A homolog of AtWRI1,GhWRI1 (accession number: JF430801), was cloned in presentstudy. Promoter activity analyses revealed that activityelements were existed in the upstream of GhWRI1 initiationcodon between -912 ~ -720 bp and -372 ~ -177 bp regions. The putative transcriptional activity domain was localized inthe C-terminal of GhWRI1 protein. GhWRI1 was moreabundant in high oil content materials and elevated expressionwith cottonseed development, suggesting the crucial role inseed oil accumulation. Meanwhile, expression level of GhWRI1was induced under multiple abiotic stresses, suggesting thatGhWRI1 might participate in cotton responding to stresses. Overexpression of GhWRI1 increased seed oil content andseed weight in transgenic Arabidopsis thaliana. In transgenicplants, genes downstream of AtWRI1, such as enoyl-ACPreductase (AtEAR, At2g05990), pyruvate kinases (AtPKp2,At5g52920), and pyruvate dehydrogenase subunit (AtPDHE1α,At1g01090) were up-regulated expression two to five timescompared with the wild-type. The results suggested that GhWRI1could be used in regulating oil accumulation in cottonseeds.
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Hongying Zhao,Jun Zhang,Haiyu Shao,Jianwen Liu,Mengran Jin,Jinping Chen,Yazeng Huang 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.3
Transforming growth factor 1 (TGF1)/Smad4 signaling plays a pivotal role in maintenance of the dynamic balance between bone formation and resorption. The microRNA miR-155 has been reported to exert a significant role in the differ-entiation of macrophage and dendritic cells. The goal of this study was to determine whether miR-155 regulates osteoclast differentiation through TGF1/Smad4 signaling. Here, we present that TGF1 elevated miR-155 levels during osteoclast differentiation through the stimulation of M-CSF and RANKL. Additionally, we found that silencing Smad4 attenuated the upregulation of miR-155 induced by TGF1. The results of luciferase reporter experiments and ChIP assays demonstrated that TGF1 promoted the binding of Smad4 to the miR-155 promoter at a site located in 454 bp from the transcription start site in vivo, further verifying that miR-155 is a transcriptional target of the TGF1/Smad4 pathway. Subsequently, TRAP staining and qRT-PCR analysis revealed that silencing Smad4 impaired the TGF1-mediated inhibition on osteoclast differentiation. Finally, we found that miR-155 may target SOCS1 and MITF to suppress osteoclast differentiation. Taken together, we provide the first evidence that TGF1/Smad4 signaling affects osteoclast differentiation by regulation of miR-155 expression and the use of miR-155 as a potential therapeutic target for osteoclast-related diseases shows great promise.
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Du, Jinping,Liang, Yi,Xin, Hangshu,Xue, Feng,Zhao, Jinshi,Ren, Liping,Meng, Qingxiang Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.11
Two separate animal trials were conducted to evaluate the coincidence of dry matter intake (DMI) and average daily gain (ADG) predicted by the Cornell Net Carbohydrate and Protein System (CNCPS) and observed actually in crossbred growing bulls kept in a traditionally confined feeding system in China. In Trial 1, 45 growing Simmental${\times}$Mongolia crossbred F1 bulls were assigned to three treatments (T1-3) with 15 animals in each treatment. Trial 2 was conducted with 60 Limousin${\times}$Fuzhou crossbred F2 bulls allocated to 4 treatments (t1-4). All of the animals were confined in individual stalls. DMI and ADG for each bull were measured as a mean of each treatment. All of the data about animals, environment, management and feeds required by the CNCPS model were collected, and model predictions were generated for animals on each treatment. Subsequently, model-predicted DMI and ADG were compared with the actually recorded results. In the three treatments in Trial 1, 93.3, 80.0 and 73.3% of points fell within the range from -0.4 to 0.4 kg/d for DMI mean bias; similarly, in the four treatments in Trial 2, about 86.7, 73.3, 73.3 and 80.0% of points fell within the same range. These results indicate that the CNCPS model can accurately predict DMI of crossbred bulls in the traditionally confined feeding system in China. There were no significant differences between predicted and observed ADG for T1 (p = 0.06) and T2 (p = 0.09) in Trial 1, and for t1 (p = 0.07), t2 (p = 0.14) and t4 (p = 0.83) in Trial 2. However, significant differences between predicted and observed ADG values were observed for T3 in Trial 1 (p<0.01) and for t3 in Trial 2 (p = 0.04). By regression analysis, a statistically different value of intercept from zero for the regression equation of DMI (p<0.01) or an identical value of ADG (p = 0.06) were obtained, whereas the slopes were significantly different (p<0.01) from unity for both DMI and ADG. Additionally, small root mean square error (RMSE) values were obtained for the unbiased estimator of the two variances (DMI and ADG). Thus, the present results indicated that the CNCPS model can give acceptable estimates of DMI and ADG of crossbred growing bulls kept in a traditionally confined feeding system in China.
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Jin, Longhai,Liu, Jinping,Wang, Shu,Zhao, Linxian,Li, Jiannan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6
Background: As a kind of common complication of the surgery of perianal diseases, perianal ulcer is known as a nuisance. This study aims to develop a kind of 20(S)-ginsenoside Rg3 (Rg3)-loaded hydrogel to treat perianal ulcers in a rat model. Methods: The copolymers PLGA<sub>1600</sub>-PEG<sub>1000</sub>-PLGA<sub>1600</sub> were synthesized by ring-opening polymerization process and Rg3-loaded hydrogel was then developed. The perianal ulcer rat model was established to analyze the treatment efficacy of Rg3-loaded hydrogel for ulceration healing for 15 days. The animals were divided into control group, hydrogel group, free Rg3 group, Rg3-loaded hydrogel group, and Lidocaine Gel® group. The residual wound area rate was calculated and the blood concentrations of interleukin-1 (IL-1), interleukin-6 (IL-6), and vascular endothelial growth factor (VEGF) were recorded. Hematoxylin and eosin (H&E) staining, Masson's Trichrome (MT) staining, and tumor necrosis factor α (TNF-α), Ki-67, CD31, ERK1/2, and NF-κB immunohistochemical staining were performed. Results: The biodegradable and biocompatible hydrogel carries a homogenous interactive porous structure with 10 ㎛ pore size and five weeks in vivo degradation time. The loaded Rg3 can be released sustainably. The in vitro cytotoxicity study showed that the hydrogel had no effect on survival rate of murine skin fibroblasts L929. The Rg3-loaded hydrogel can facilitate perianal ulcer healing by inhibiting local and systematic inflammatory responses, swelling the proliferation of nuclear cells, collagen deposition, and vascularization, and activating ERK signal pathway. Conclusion: The Rg3-loaded hydrogel shows the best treatment efficacy of perianal ulcer and may be a candidate for perianal ulcer treatment.
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Xuesong Shang,Gongren Hu,Chi He,Jinping Zhao,Fuwang Zhang,Ya Xu,Yunfeng Zhang,Jianrong Li,Jinsheng Chen 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.1
An effective and applicable method for the regeneration of used commercial full-scale honeycomb monolith catalysts in China (V2O5–WO3/TiO2) is firstly reported. The de-ionized water and dilute sulfuric acid solution with a relatively low concentration (pH = 2) are potent reagent to regenerated the catalysts used for about 30,000 h in coal-fired power plant. All samples were systematically characterized by TEM,N2 adsorption/desorption, EDX, FT-IR and XPS techniques. The evaluation results reveal that the activity of the used catalysts can be obviously improved after regeneration processes. Moreover, the regenerated catalysts possess superior activity under different gas hourly space velocity (GHSV), and SO2 oxidation rate is lower than 1% in all cases. The regeneration method can remove the deposits, restore active sites and prevent nanoparticles aggregation as well as active phases leaching. Overall, these processes have promised as effective approaches for industrial V2O5–WO3/TiO2 catalysts regeneration.
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Xin Caiyan,Wang Fen,Zhang Jinping,Zhou Quan,Liu Fangyan,Zhao Chunling,Song Zhangyong 한국미생물학회 2023 The journal of microbiology Vol.61 No.2
Candida spp. and Cryptococcus are conditional pathogenic fungi that commonly infect immunocompromised patients. Over the past few decades, the increase in antifungal resistance has prompted the development of new antifungal agents. In this study, we explored the potential antifungal effects of secretions from Serratia marcescens on Candida spp. and Cryptococcus neoformans. We confirmed that the supernatant of S. marcescens inhibited fungal growth, suppressed hyphal and biofilm formation, and downregulated the expression of hyphae-specific genes and virulence-related genes in Candida spp. and C. neoformans. Furthermore, the S. marcescens supernatant retained biological stability after heat, pH, and protease K treatment. The chemical profile of the S. marcescens supernatant was characterized by ultra-high-performance liquid chromatography–linear ion trap/orbitrap high resolution mass spectrometry analysis and a total of 61 compounds with an mzCloud best match of greater than 70 were identified. In vivo, treatment with the S. marcescens supernatant reduced the mortality of fungi-infected Galleria mellonella. Taken together, our results revealed that the stable antifungal substances in the supernatant of S. marcescens have promising potential applications in the development of new antifungal agents.
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Yongxian Hu,Jiasheng Wang,Chengfei Pu,Kui Zhao,Qu Cui,Guoqing Wei,Wenjun Wu,Lei Xiao,Yang Xiao,Jinping Wang,Zhao Wu,He Huang 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4
Chimeric antigen receptor T-cell strategy targeting CD19 (CART19) has prominent anti-tumor effect for relapsed/refractory B-cell lymphomas. CART19-associated complications have been gradually recognized, however, late-onset complications have not been extensively studied. Herein, for the first time we report a diffuse large B-cell lymphoma patient with terminal ileum involvement obtained rapid remission and developed spontaneous terminal ileal perforation 38 days following CART19 infusion. The late-onset perforation reminds us that, for the safety of CART treatment, more cautions are warranted for the management of delayed GI complications.
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