Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

정신분열병의 임상적 고위험군의 특징 : '서울청년클리닉'의 자연 추적 관찰 예비연구

유소영,이경진,강도형,이승재,하태현,위휘,이애라,송지연,김성년,권준수 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.1

Objectives : The aims of this study were to identify the intake and following process for subjects at high risk of transition to schizophrenia and to examine the neurocognitive, social, and emotional functions of the subjects compared with healthy controls. Methods : Symptomatic individuals judged at high risk for schizophrenia ('clinical high risk') within Youth Clinic were assessed and followed up. They performed a neurocognitive function test, a social function test and an affective function test. Twenty healthy controls were recruited in this study. Results : Among eighty-two subjects contacted through the Youth clinic, sixteen subjects were judged as the clinical high risk group. Fourteen subjects among the clinical high risk group showed deficits in several domains of neurocognitive functions, such as visual recall memory, verbal short term memory and executive function. Social and affective functions are also impaired in the Cli-nical high risk group compared with healthy controls. Two of 15 subjects (13%) developed a psychotic disorder within 6 months. Conclusion : This study illustrates the state of follow-up study for a clinical high risk group. Despite low numbers and short durations, some impairment of several functions in the clinical high risk group suggests possible predictors of psychosis.

치조골 복합골절의 정복고정시 탈구치아의 치근관을 통한 배농술의 효과

유재하,김현실,유태민,이지웅,김종배 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.5

Treatment of compound alveolar bone fracture consists of reduction and fixation with wound closure of soft tissue laceration. For the proper reduction and fixation, interosseous wiring or splinting of the involved teeth are applied during about 4 weeks. But wound infection around the fracture site may be occurred, because of the presence of devitalized tissues(pulp, bone and soft tissue), residual hematoma and anergy effect of trauma stress. The wound infection results in bone resorption, pulp degeneration and necrosis, wound dehiscence and soft tissue necrosis around the fracture site. Therefore, if the infection will be expected, early proper drainage should be established via the fracture site and root canal of the luxated teeth. The authors treated two cases of compound alveolar bone fracture by the rubber drainage in the fracture site and the endodontic drainage by open canal of the luxated teeth, with the usual reduction and fixation by use of arch bar and resin. The prognosis was more favorable without wound infection, bone resorption, teeth mobility and specific root resorption.

내시경적 절제로 진단된 폴립 형태의 심재성 낭종성 위염 2예

허종현,조영화,성용완,유재훈,구동영,김갑식,노지훈,문원 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

Gastritis cystica profunda (GCP) is a disease characterized by hyperplastic and significant extension of cystic dilatation of the gastric mucous glands, which results in a cystic lesion in gastric submucosa. It often occurs on the part of gastroenterostomy, but can be found in the stomach without any previous surgery. GCP has variable gross finding including solitary polyps, diffuse ones, submucosal tumors, and rare giant gastric mucosal fold. It is difficult to tell GCP from a cancerous lesion by gross finding that the disease demands a necessary tissue biopsy, though GCP is commonly showed as a benign in the progress. We report a case of GCP in polypoid types diagnosed by endoscopic polypectomy.

소프트웨어 품질 보증을 위한 평가항목별 가중치 계산 알고리즘

유지현,이병걸 서울여자대학교 자연과학연구소 2001 자연과학연구논문집 Vol.13 No.-

To produce reliable and high quality software, software quality assurance is very important in relation to software evaluation process. One of the key issue to be considered in quality evaluation is how to achieve objectiveness and quantitative measurement in evaluation process. A typical evaluation algorithm relies on experts' assured. In this paper, we propose an alternative algorithm for generating weight value on evaluation criteria which represents the degree of importance among evaluation characteristics conforming to ISO9126 standards. Especially, Dempster-Shafer theory is used to enumerate the hierarchical relationship among quality characteristics, and thus generate quantitative value from qualitative information such as evaluator's judgement or knowledge.

각이등분법 및 평행법에 의한 전악구내 표준 촬영시 두경부 피부 흡수선량 비교

유명진,김현자,도시홍,나경수,김애지 大韓口腔顎顔面 放射線學會 1990 Imaging Science in Dentistry Vol.20 No.2

This study was performed to measure the skin absorbed doses from full mouth standard intraoral radiography(14 exposures) in bisecting angle and paralleling techniques. Thermoluminescent dosimeters were used in a phantom. Circular tube collimator(60mm in diameter, 20cm in length) and rectangular collimator(35mmX44mm, 40cm in length) were set for bisecting angle and paralleling techniques respectively. All measurement sites were classified into 8 groups according to distance from each point of central rays. The results were as follows: 1. The skin absorbed doses from the paralleling technique were significantly decreased than those from the bisecting technique in both points at central ray and points away from central ray. The percentage rates of decrease were greater at points away from central ray than those at central ray. 2. The skin absorbed doses at the lens of eye, parotid gland, submandibular gland and thyroid region were significantly decreased in paralleling techniuqe, but those of the midline of palate remained similar in both techniques. 3. The highest doses were measured at the site 20mm above the point of central ray for the mandibular premolars in bisecting angle technique and at the point of central ray for the mandibular premolars in paralleling techniques. The lowest doses were measured at the thyroid region in both techniques.

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

갑상선 종양에서 thrombin receptor (PAR-1)의 발현 양상

박지영,목지오,김여주,박형규,김철희,변동원,서교일,유명희,조현득,오미혜,김상진 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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Free Communications : P2 ; Cannabinoid Receptors : Its Impact in Epidermal Differentiation and Possible Role in Treatment of Psoriasis

( Ji Seok Kim ),( Chan Hee Nam ),( Ji Yeon Yoo1 ),( Hyun Jung Kim ),( Se Kyoo Jeong ),( Sung Ku Ahn ),( Seung Phil Hong ) 한국피부장벽학회 2014 한국피부장벽학회지 Vol.16 No.2

Purpose: There are researches indicating that in human skin, the cannabinoid receptors exist, which take a part of endocannabinoid system. Nonetheless, the possible association of cannabinoid receptors with the pathogenesis of psoriasis has not yet been fully elucidated. Through this research, we tried to document the association between cannabinoid system, epidermal differentiation and psoriasis. Methods: Using human keratinocyte (KC), the expression of cannabinoid receptor-1 (CB1R) and -2 (CB2R) was analyzed according to the degree of differentiation. Also we examined for changes in differentiation marker of KC after application of CBR agonist. In addition, murine model applied with imiquimod to induce psoriasis symptoms was used to evaluate the effect of topical CBR agonist on inflammation and skin barrier function. Results: Compared to normal human skin, CBR expression was reduced in epidermis of psoriasis skin. Western blotting revealed the expression of both CB1R and CB2R in undifferentiated KC. The expression level of CB1R increased as the differentiation progressed in KC as with involucrin, K1 and K10. On the other hand, there was no change of CB2R in the process of differentiation. Furthermore, CB1R agonist partly increased expression of proteins associated with epidermal differentiation. Through this we could speculate that the CB system may be associated with proteins related to epidermal differentiation. Moreover, inflammation and barrier function in murine models with psoriasis symptoms were improved after 3 days-application of topical CB1R agonist. Conclusion: Cannabinoid receptors in human skin might be related to the differentiation of KC, and since its activation could improve skin barrier condition in murine psoriasis model, it can be expected to be a new therapeutic target for treatment of psoriasis.

권운과 대기의 복사수지: 열대해양에서의 권운의 모사 및 광학적 성질

유정문,이지현 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-

The spectral data obtained from Infrared Interferometer Spectrometer(IRIS) have been simulated in order to examine optical features of cirrus clouds in the tropical oceans. The sensitivity experiments of upwelling intensity to several optical and meteorological factors have been done using the models of the Mie and multiple scattering. The intensity in the experiments is highly sensitive to the particle size and ice-water content (or optical depth) of clouds, while it is less sensitive to their phase and altitude. From theoretical simulation of the infrared spectra the tropical cirrus clouds generally have a two-layer vertical ()tratification in particle size, with larger particles(∼12㎛)in their bottom and smaller ones(∼6㎛) aloft. The vertical structure of these clouds differs from those of the cirrus clouds in polar regions, in that the polar cirrus clouds have an upper layer of smaller particles(≤1㎛). Among 400-1,250() infrared region, the scattering effect due to cloud particles is strong in the relatively short wavelength of 1,050-1,250() portion, while the absorption effect is predominant in the other spectral region. The forward scattering due to the cloud particles for upwelling intensity is the strongest in the large cloud particle size and optical depth. In addition, the scattering effect in the forward direction of water particles generally is a little stronger than that of ice. The cirrus clouds which have been distributed globally and extensively are very persistent and play an important role in the earth's climate by influencing the heat balance and transport in the earth-atmosphere system.