도토리 추출물의 3T3-L1 세포 분화억제 효과

김지연,이진,이창원,김애정,Kim, Ji-Yeon,Lee, Jin,Lee, Chang-Won,Kim, Ae-Jung 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

본 연구에서는 만성질환의 주요 위험인자인 비만을 예방하기 위한 식품소재로서 3T3-L1세포를 이용하여 탈피한 도토리의 항비만 효과를 알아보고자 하였다. 3T3-L1 세포에서 생존율(MTT assay)을 측정한 결과, AE와 AW 시료 모두 $500{\mu}g/mL$ 농도에서는 다소 생존율의 감소를 보여, $300{\mu}g/mL$를 최종 농도로 정하였다. 3T3-L1 세포의 지질축적 억제 효과를 측정한 결과, 농도 $100{\mu}g/mL$로 처리하였을 때 두 시료 모두 지질축적량의 증가를 보였으나, $200{\mu}g/mL$ 처리농도에서 AE 시료는 82%로, AW 시료는 74%로 감소되다가 $300 {\mu}g/mL$ 농도에서는 두 시료 모두 약 53% 수준까지 지질축적이 억제되었다. 3T3-L1 세포에서 중성지방 억제 효과를 확인한 결과, AE 시료의 경우 $200{\mu}g/mL$ 농도에서 11%의 감소율, $300{\mu}g/mL$ 농도에서 42% 수준의 감소율을 보였다. AW 시료도 $200{\mu}g/mL$ 농도에서 5%의 감소율과 $300{\mu}g/mL$에서 41%의 감소율을 보였다. 3T3-L1 세포의 ROS 생성량을 측정한 결과, 시료 농도 $200{\mu}g/mL$에서 AE는 42%, AW는 33%로 $300{\mu}g/mL$에서는 AE는 58%, AW는 52%로 ROS 생성량의 억제를 보였다. 3T3-L1 세포에서 mRNA 발현에 미치는 영향을 대조군과 비교하였을 때 두 시료(AE와 AW) 모두 $300{\mu}g/mL$ 농도에서 $PPAR-{\gamma}$은 54%와 38%, aP2는 40%와 18% 수준의 발현을 억제시키는 것으로 나타났다. 이상의 결과로 볼 때 탈피한 도토리는 3T3-L1 세포의 분화를 억제함으로써 새로운 항비만 소재로의 가능성이 있는 것으로 판단된다. This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

Acute exacerbation of interstitial lung disease; subgroup analysis for causes, risk factors for mortality rate, and steroid responsiveness

( Seung Hyun Yong ),( Ah Young Leem ),( Song Yee Kim ),( Kyung Soo Chung ),( Ji Ye Jung ),( Young Ae Kang ),( Young Sam Kim ),( Joon Chang ),( Moo Suk Park ),( Hye Jin Jang ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Background: Interstitial Lung Disease (ILD) has known as a poor prognosis disease, particularly idiopathic pulmonary fibrosis (IPF). Corticosteroid is widely used for treatment of acute exacerbation (AE)-ILD. This study aims to clarify efficacy of corticosteroid use, and compare the mortality rate depending on the subgroups of ILD. Materials and Methods: We collected the data from the patients who have diagnosed ILD and visited emergency room (ER) with the respiratory symptoms from January 1, 2016 to December 31, 2018. We classified AE-ILD patients into 3 groups depending on methylprednisolone dose per kilogram. Also, we compared the mortality of IPF patients to that of non-IPF patients. Results: A total case of AE-ILD visited ER from 2016 to 2018 was 209 cases, including IPF cases (n=122) and non-IPF cases (n=87). We divided total patients (n=209) into three groups depending on steroid use, and these include group 1 of 0-0.49 mg/kg (n=78), group 2 of 0.5-0.99 mg/kg (n=58), and above 1.0 mg/kg (n=41), each. We found poor prognosis from the cases of IPF compared to non-IPF cases (p=0.029, OR 2.463). Furthermore, there was significant differences of mortality rate according to steroid dose (p=0.036, OR 1.712), CRP elevation (p=0.016, OR 1.004), and intubated case (p=0.000, OR 8.152). Also, we categorized IPF (n=122) to non-IPF cases (n=87) and analyzed the mortality rate. Most of the patients were death from ILD itself (n=23, 63.9%), Pneumonia(n=8, 22.2%), and Lung cancer (n=5, 13.9%). Bacterial infection (n=26) was dominant cause of AE, followed by fungal infection (n=15), and viral infection (n=10). Conclusions: This retrospective analysis of corticosteroid treatment shows more fatal outcome when we use high dose steroid in AE-ILD cases, and there was significant difference in mortality rate depending on the subgroup of ILD and medical background, such as intubation status, higher CRP level, and IPF cases.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

정수공정에서 이취미물질 제거에 관한 연구

김지태,한명애,곽현,배성렬 漢陽大學校 工學技術硏究所 2000 工學技術論文集 Vol.9 No.1

이취미물질중에서 페놀, Geosmin, 2-MIB를 선정하여 응집제(PACS)와 흡착제(PAC) 및 산화제(Cl2, ClO2)로 처리하였을 때 제거율을 알아보았다. 페놀의 경우 이산화염소(ClO2)와 분말활성탄(PAC)으로 처리하였을 때 제거효율이 높았고, Geosmin과 2-MIB는 분말활성탄(PAC)으로 처리하였을 때 제거효율이 높았다. 그러나 페놀, Geosmin, 2-MIB 모두 응집제(PACS), 염소(Cl2)로 처리하였을 경우에는 제거효율이 거의 없음을 알 수 있었다. The removal ratio of phenol, geosmin, and 2-MIB selected in odor and taste causing materials was investigated, when water was treated with coagulant(PACS), adsorbent(PAC), and oxidants(Cl2, ClO2). Phenol was removed at a relatively higher rate by the treatment with CIO2 and PAC(Powder Activated Carbon). The other side, geosmin and 2-MIB were removed at a higher rate by the treatment with PAC. But it was found that the treatment with coagulant(PACS) and chlorine(Cl2) for the removal of phenol, geosmin and 2-MIB was little removal efficiency.

삶의 질 향상과 지속가능한 지역경쟁력 : 호순환 관계의 모색

장지상,이성진,조정재,권우현,김애경 慶北大學校 1997 經商論集 Vol.25 No.1

두경부 전산화 단층촬영시의 주요 장기선량, 유효선량 및 위험도

김애지,나경수,조봉혜 大韓口腔顎顔面 放射線學會 1995 Imaging Science in Dentistry Vol.25 No.1

The organ or tissue doses were determined with head and neck phantom measurement for multiple axial scans (36 slices), multiple coronal scans (13 slices), 3 types of single axial scans(orbit, maxillary sinus and mandibular canal) and single coronal scan(maxillary sinus). For each scan sequence 30 TLDs were placed in selected sites(16 internal site and 14 external sites) in a tissue-equivalent phantom. The exposure was made at 120kVp, 500mAs with 5 mm slice width. The results were as follows: 1. In multiple axial scans, the greatest effective dose recorded was that delivered to the thyroid glands(2.77 mSv) and the least was that received by the skin(0.05 mSv). From these data, stochastic effects were ?? and ??, respectively. 2. In multiple coronal scans, the greatest effective dose recorded was that delivered to the salivary glands(0.58 mSv) and the least was that received by the skin(0.01 mSv). From these data, stochastic effects were ?? and ??, repectively. 3. Among single axial scans, the greatest effective dose recorded was that delivered to the salivary gland(0.38 mSv) in maxillary sinus scan. From this data, stochastic effect was ??. 4. In single coronal scan, the greatest effective dose recorded was that delivered to the salivary gland(0.01 mSv). From this data, stochastic effect was ??. 5. The equivalent dose measured that delivered to the lens of the eyes was 69.64 mSv in multiple axial scan, 39.32 mSv in multiple coronal scan and 36.77 mSv in single axial scan(orbit).

일 방문보건사업 대상노인들의 건강상태, 우울, 자아통합감 및 방문에 대한 만족도 조사 : an evaluation study for a community health visiting program

유애광,김옥란,정경옥,강지연 김천과학대학 1999 김천과학대학 논문집 Vol.25 No.-

The objectives of this study are to explore the health status, depression and self-integration of living-alone elderly and to evaluate a community health visiting program managed by nursing faculties and students. Study subjects were 28 residential or institutional living-alone elderly who were the clients of community health visitng program in department of nursing, Kimcheon Science College. Each data was collected through an interview with the nursing students who visit the client's home once a week. The major findings of the study were as follows: (1) About 89% of study subjects considered they were not in good health status and those who thought they were unhealthy compared to other people experienced more accident, trauma or disease. (2) The depression scores were negatively correlated with self-integration scores. Those who benefit governmental support show higher depression and lower self-esteem than that of economically independent subjects. The higher health status they were in, the lower depression and higher self-integration they felt. (3) Most subjects expressed satisfaction to the home visiting services offered by nursing students. However, the satisfaction rate for visible outcome, relevancy of service and nursing skill were relatively lower than other items like preference, attitude of students or visiting itself. In conclusion most living-alone elderly are in low physical and psychological health state, and health promotion programs like home visiting services should be developed.

초등학교 민속무용 교육 과정에 관한 연구

이애현;강지영 한국무용연구회 2007 한국무용연구 Vol.25 No.1

This study is aimed to review whether the text book of Physical education in primary and high school has been focused on “The Continuity on education” and whether it has been doing the bridge role for proceeding to higher level dance education, by analysing in detail the chapter “Korean Folk Dance” in the 7’th education programme(text book). It was checked whether there is a certain continuity between the education target and the content of text book, while, the way for improvement was searched in order to maximize the education effect. The study is conducted also to provide the helpful information for the 8'th education programme. As a result of analysis, we first found that the education target of the primary and high school was not realistic, not constructive/ systematic according to grades and chapters and there were also no differences on the education target by grades. It was also known that the way of selection for folk dance programme was same in both text books while the target of education was not constructive without consideration of the learning progress of students and, therefore, the content was not enough/ suitable to fulfil the aim for art education value/ target. The detailed review shows that the content for Korean folk dance mainly deals with functions only without considering the interest of student and does not basically meet the principle specialities of Korean folk dance, I. e, “Gathering together and Enjoying with joy.” On the other hand, the high school text book takes the content of the same of primary school as it is, which was also contradicting to the education aim of “Various experiences” being emphasized in high school. The continuous repeats of simple content can eventually make the education (learning) will of student decreased and can further give just confusions to student on the concept of education by unmatching contents of books for different schools. Therefore, a certain guide line with clear principle must be given for the contents of text books in order to provide the continuity in between primary and high school while the remake/ modification of text book are required, so that it can provide systematic and constructive education, according to the learning procedure of students. 본 연구는 초·중·고등학교간의 연계적인 학습으로써 초등학교 체육 교과 내 무용교육의 가치에 역점을 두고 초등학교 3•4•5•6학년 『체육』 교과서 내의 '우리나라 민속 무속 단원을 중심으로 분석하였다. '우리나라 민속무용'단원0| 교과목표체계와 내용체계 면에서 연계성을 갖고 있는지에 대해 살펴보고, 학습자의 교육적 효과를 극대화시키는데 있어서의 문제점을 지적하여 개선방안을 모색하였다. 분석 결과, 초등학교 학년별·단원별로 학습목표가 비현실적이며 구체적으로 세분화 되어 있지 않았고, 학년별 목표의식의 차별성이 없음을 알 수 있었다. 구체적으로 제시하면 초등학교 교과서에는 동일한 민속무용의 프로그램이 중복 수록되어 있었고, 학습자의 발달과정을 고려하지 않은 목표 및 내용 선정, 그리고 단원의 소개에 그친 나열식의 교과 내용, 예술교육으로써의 가치와 목표에 부합되지 않는 내용 수록 등 문제점들이 지적되었다. 즉, 우리나라 민속무용의 교과내용은 학생들의 흥미를 고려하지 않은 기능중심의 교과 내용으로 편성되어 있으며. 우리나라 민속무용의 특성인 함께 어우러지고 흥겹게 즐기기 위한 목표에 부합되지 않는 것으로 나타났다. 따라서 초등학교 무용교육 과정간의 연계성을 갖도록 목표설정과 내용선정에 타당한 근거 및 기준점을 마련하기 위해 학습자의 발달상황에 맞는 단계적이고 체계화된 교과서의 개정이 요구되며 개정 방향은 다음과 같다. 첫째, 목표체계 면에서는 성장발달 단계에 따른 적합한 목표설정으로 학습자의 성취기준을 높여주며, 흥미위주의 교육내용으로 편성해야 할 것이다. 둘째, 내용체계 면에서는 다양한 프로그램의 개선0| 시급하며, 교육프로그램화 시킬 수 있는 민속무용의 발췌도 함께 이루어져야 한다. 따서 각 학년별로 단원별·차시별 목표와 내용에 적합한 기초·기본·전보의 세가지 학습단계를 적용한 학습체계가 마련되어야 할 것이다.

중증 화상 환자에서 영양지원

박지영,목혜숙,신현선,노순예,김애심,백금선,김도헌,허준,전욱 대한화상학회 2003 대한화상학회지 Vol.6 No.2

신경세포의 Outgrowth 향상을 위한 마이크로 파이버 지지체와 전단응력의 영향

김인애,박수아,김영직,김수향,신호준,이용재,신지원,신정욱 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

We introduced mechanical stimuli and micro patterned substrate with micro fibers to investigate the effects of those on neurite outgrowth along with nerve growth factor (NGF) in vitro. Micro fiber substrates were fabricated using an electros pinning process. And PC-12 cells cultured on substrates were simulated with newer growth factor and laminar flow shear stress in a fluid flow system. The results suggest that micro fiber substrates and fluid-induced shear stress are promising for simulating neuronal regeneration in a desired direction.