대두 식이섬유를 이용한 특수 영양식품의 유효성 평가

한재흠,이균희,손현수,이윤복,박점선,오만진 충남대학교 농업과학연구소 2008 농업과학연구 Vol.35 No.1

In the development of enteral foods for the patients with diarrhea, soybean hull, by-products of soybean processing, was used to prepare crude dietary fiber extracts (soybean hull fiber, SHF). Total dietary fiber content of SHF is 85% and their composition are 86.1% cellulose, 8.1% hemi-cellulose, and 4.7% lignin. The effects of SHF on the prevention of diarrhea were studied in animal. Spraque-Dawley (SD) rats were fed AIN93G diets containing 5% dietary fiber for 3days simultaneously inducing diarrhea with the phenolphthalein Mg citrate solution. On day 4, feces were collected at different time point. Dietary fibers used for the animal study were SHF, soybean cotyledon fiber (SCF), psyllium husk fiber (PHF), and chicory fiber (CF). α-cellulose was used as a control. Body weight gain, calorie consumed and food efficiency ratio among the experimental groups were not different. However, water content in the feces of SHF group was significantly lower by 10%, compared with other groups at 24hrs. time point. This effect was even greater in the feces collected later than 24 hrs. time point. SHF seems to have a greater effects on slow the symptom of diarrhea. Based on the previous results, enteral food enriched with SHF were prepared and its effect was compared with other commercially available products from domestic or imported ones. Weight changes among experimental groups were not different, but the moisture content of feces consumed SHF enriched products were lower than that of other products. Approximately 10% decrease in water content was observed from feces collected at 24 hr time point. According to the sensory evaluation, overall acceptability of the enteral food enriched with SHF was 3.24 out of 5 indicating that taste of this product is acceptable. 대두가공에 부산물로부터 얻어지는 대두 식이섬유를 이용하여 설사환자를 위한 경장 영양 식이를 개발하기 위하여 쥐에게 투여한 후 체중증가율, 변의 수분감소, 사료효율 등을 측정하여 다음과 같은 결과를 얻었다. 대두피 중의 식이섬유함량은 85% 이었고 cellulose 가장 높았으며 hemi-cellulose, lignin의 순 이었다. 대두 식이섬유가 대조구에 비하여 체중 감소율은 약간 낮았고 사료효율은 약간 높았다. 대두 식이섬유를 섭취시킨 쥐가 치커리 식이섬유, 차전자 피에 비하여 24시간 후의 변의 수분함량은 10% 낮았다. 대두 식이섬유를 첨가한 경장영양식이로 사육한 쥐의 체중변화와 사료효율은 3제품 간에 차이가 없었으나 다른 하나의제품은 대조군에 비하여 낮았다. 대두 식이섬유를 이용하여 가공한 경장영양식이 다른 상업용 제품에 비하여 실험쥐의 변 수분함량은 10% 이상 낮았다.

癌患者 診療 및 放射線治療에 관한 統計的 考察(Ⅲ) : 일부 地域을 中心으로

한재진,김승국,김창복 광주보건대학 1999 論文集 Vol.24 No.-

The paper is based on the record of researching the patients with cancer in the Chunnam National University Hospital from January 1994 to December 1997. The results are as follow ; 1. Among the total O.P.D 2,452,013, the patients of therapeutic radiology are classified into 86,584(3.53%). (20,641 in 1994, 19,597 in 1995, 22,097 in 1996, 24,249 in 1997) 2. Among the 5,571 cancer patients, stomach cancer patients are 872(15.65%), Liver cancer patients are 785(14.09%), Lung cancer patients are 554(9.94%), Cervix and Uterus cancer patients are 392(7.04%), Colon and Rectum cancer patients are 362(6.50%), Breast cancer patients are 307(5.51%) and Sexual ratio appeared 1.70 : 1. therefore, male patients are much more than female patients. 3. The age distribution of cancer was on the 55∼64 age 1,781(31.97%), 65∼75 age 1,176(21.11%), 45-54 age 1,141(20.48%). Therefore, 45∼75 age was the 70% of all cancer patients over. 4. Among the 3480 therapeutic radiology patients, 684(19.66%) are in the uterus system, 636(18.28%) are in the lung, 615(17.67%) are in the head and neck part. Therefore those three kinds of therapeutic radiology patients consist of 50% more. 5. Among the intracavitary application therapy of 520 cervix cancer patients are 173 in 1995(33.3%), 162 in 1996(31.2%), 185 in 1997(35.6%) and the patients of medical insurance are 75,874(87.6%). 6. The occupational distribution of 5,571 cancer patients(94∼97 year), Oderly Farmer, Stock Breeder, Fishery and Forestman are 1,395(25.04%), House wives are 1,210 (21.7%), Businessmen are 263(4.72%), Public Service Personnel and Salariedmen are 3.66%, 3.59% each.

서남권도시의 인구지표모형 검증에 관한 연구

오재화,이재복,박한생 朝鮮大學校 建設技術硏究所 2007 建設技術硏究 Vol.27 No.1

The problem of over population in large cities and over outflow population in cities, and the difference of growth and unbalanced development between them occurred as a big social issue. Accordingly, the interests are concentrating on the basic reinforcement and functional activation of local cities. I can assert that it is the optimum time to smooth out difference between regions, to activate local small and medium cities at this time. Because the recognition against polarization reversal on the dispersal policies then the first stage of the powerful trend of concentrative development. The basic goal of this treatise puts on emphasize to issue the problems through looking into realities and functional roles of cities in the urban system for the past few years in korea, to find out alternative method in order to activate the strengthening of foundation and functional activity of local cities by analyzing the relevancy between functional character and growth potentiality of individual cities.

Baculovirus를 이용한 Aujeszky's Disease Virus gⅢ 단백질 발현

송재영,이중복,현방훈,박종현,김병한,권창희,전무형,안수환 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

The g Ⅲ gene located in U_L region of Yangsan strain, a field isolate of Aujeszky's disease virus (ADV) in Korea, was cloned into pTZ18R and sequenced. The gⅢ gene consisting of 1,437 nucleotides showed 98% sequence homology with that of Becker strain, a reference strain of ADV. The gene encoding gⅢ of Yangsan strain was placed under the control of Autographa californica nucleopolyhedrosis virus (AcNPV) polyhedrin promoter, and expressed by the derived recombinant baculovirus using Spodoptera frugiperda 9 (Sf9) cells. The expressed gⅢ was a protein with molecular weight of 72kd determined by immunoprecipitation and Western blotting assay using anti-ADV polyclonal antibodies and anti-gⅢ monoclonal antibody. The partially purified gⅢ protein was utilized as antigen in the radial immunodiffusion enzyme assay (RIDEA) to detect to specific antibody against ADV in pig sera. The results indicated that the sensitivity of RIDEA with the recombinant gⅢ protein antigen (98%) was as high as that with the conventional glycoprotein antigen extracted from the ADV infected cells. In addition, the false positive and false nagative reactions in gⅢ RIDEA were significantly reduced than the conventional glycoprotein RIDEA as judged from the results of standard serum neutralization test.

고추의 品質을 결정하는 成分形質에 對한 Heterosis表現과 組合能力

朴載福,韓東旭 서울市立大學校 1984 論文集 Vol.18 No.-

Since the discovery of genetic-cytoplasmic made sterility of hot pepper, many F₁varieties were come out in Korea. Thus, to clarify the inheritance mode of some important quality characters of hot pepper, heterosis, combining ability and gene actions were estimated with diallel crosses among 6 hot pepper varieties. The materials were grown in the field and were served for genetic analysis. The results of F₁diallel cross for some quality characters were as follows. 1. In carotenoid content, significant heterosis was showed, while it was not showed in capsaicin, vitamine C, sugar and free amino acid content. 2. The continuous variation was obserbed in the quality characters of hot pepper such as capsaicin, carotenoid, vitamine C, sugar, and free amino acid content. 3. Besides the additive control, some combinations showed the epistasis or complementary control of polygene, and dominant major gene seemed to be effect, too. 4. The mean squares of general combining ability (GCA) for all studied characters were highly significant, but those of specific combining ability (SCA) were not significant. 5. Capsaicin content were positively correlated with carotenoid content while it was not negatively correlated with vitamin C, sugar, and free amino acid content. 6. Fruit weight was negatively correlated with capsaicin and carotenoid content, while it was negatively correlated with vitamine C, sugar, and free amino acid content. 7. To improve the F₁ hybrid with large fruit and high capsaicin content, selection of breeding materials was indispensable problem. This work was also showed possibility to breed the high quality varieties contained a plenty of vitamine C and amino acid.

한국산 Semisulcospira gottschei의 배 발생에 따른 전기영동적 연구

김창한, 유재복 忠北大學校 1986 論文集 Vol.32 No.-

-본문참고-

팩시밀리의 主機板에 對한 技能的 試驗機의 設計 및 具現

김신택,이재훈,김명선,김한상,민형복 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

본 연구는 팩시밀리의 주기판(main board)을 기능적으로 테스트하기 위해 구현한 FFTS(Functional Facsimile Test System)에 대한 기술이다. 본 연구의 목적은, 테스트시 수반되는 모든 작업을 자동화하고, 테스트 수행중 발생하는 여러가지 상태들을 자료화하는 것이다. 아울러 주기판에 대한 입출력 신호들의 종류와 그 특성들이 변경될 경우라도 소프트웨어의 수정만으로 그 것에 대처한 후 계속적으로 테스트를 수행할 수 있도록 한다. FFTS는 주기판에 접속되는 여러가지 장치들을 하드웨어적으로 에물레이션하며, 테스트용 전용언어, 그리고 그것에 대한 컴파일러를 포함한다. FFTS로 테스트를 수행하는 방법에는 대화식 모드(IAT)와 대화식 테스트에서 수행할 수 있는 모든 과정을 테스트용 언어로 저장한 후 그것을 반복하여 사용하는 배치 모드(Batch mode) 등이 있다. FFTS의 유용성을 실증하기 위해 여러 종류의 팩시밀리에 대하여 실험을 실시하였다. FFTS (Functional Facsimile Test System) is designed and implemented. It is possible to eliminate repeated test work and to analyze the data obtained through test. If the specification of the signals from/to main board of facsmile is changed, it is possible to cope with the change by modification of FFTS software. FFTS emulates the devices connected to the main board. FFTS is designed to test the main board either by using interactive(IAT) mode with graphic user interface or by using noninteractive(NIAT) mode with test command file which is coded manually or created from IAT mode. The operation of the FITS is verified for several types of main board of G3 class facsmiles.

정수 슬러지의 고화처리에서 황토 및 첨가재의 영향에 관한 연구

임성진,김한,이재복 대한상하수도학회 2003 상하수도학회지 Vol.17 No.6

The purpose of this research is to investigate the feasibility of the modified waterworks sludge with loess as cover soil in sanitary landfill site. Modified sludge was experimentally produced with fly ash, cement, loess, and activated loess dosing. Uniaxial compression strength of modified sludges showed material property improvement applicable for soil cover alternatives. Heavy metals were analyzed in terms of leaching characteristics using techniques of Korean Standard Procedure and Toxicity Characteristics Leaching Procedure. Leaching tests for hazardous components in modified sludge revealed below the regulated criteria. When adding 2030% activated loess to water treatment plant sludges, the modified sludges could reach the compression strength for cover soil after 7 days solidification.

노령에서 고관절 골절에 영향을 주는 수상전 요인

김종오,윤여헌,고영도,유재두,정준모,방한천,강규복 대한골절학회 2002 대한골절학회지 Vol.15 No.4

목 적 : 노령에서 고관절골절의 여러 원인적 요인들과 상관관계를 분석, 그 연관성에 대해 알아 보고자 하였다. 대상 및 방법 : 2001년 1월부터 2002년 5월까지 대퇴 고관절 골절로 본원에서 치료받은 환자중 60세 이상이고 골밀도 검사를 시행받은 158례를 대상으로, 수상전 요인을 나이, 성별, 흡연여부, 비만정도, 골절종류, 수상장소, 다른 이환 질환, 골절전 활동도, 골밀도 검사, 수상당시 바닥의 경도, 수상당시 넘어진 방향, 수상에너지에 따라 대퇴 경부골절과 전자간 골절, 전자간 골절중 안정골절과 불안정 골절로 나눠 비교하였다. 결 과 : 80세 이상의 군에서 대퇴 전자간 골절이 대퇴 경부 골절에 비해 의미있게 많이 발생했음을 알 수 잇었으며(p<0.001) 환자의 수상전 활동정도의 비교에서도 활동정도가 낮았던 군에서 대퇴 전자간 골절이 의미있게 많이 발생하였다(p<0.001). 골밀도 검사에 따른 비교에서는 T-score -3.0 이하인 군에서 대퇴 전자간 골절이 많았으며(p<0.005), 수상에너지에 따른 비교에서는 적은 에너지에 의한 골절이 전자간 골절에서 많았다(p<0.05). 대퇴 전자간 골절은 안정 골절과 비안정 골절로 나눈 비교에서는 사회 활동이 어려운 ADL C이하의 군과(p<0.05), 골밀도 T-score -3.0 이하인군에서는 의미있는 차이를 보였다(p<0.001). 결 론 : 노령에서의 고관절 골절 중 80세 이상의 고령에서, 골밀도 T-score -3.0 이하인 경우, 일상생활 활동정도가 낮은 경우에, 비교적 적은 에너지에 의한 충격시에 대퇴 전자간 골절의 발생이 대퇴 경부 골절에 비해 의미있게 많았고 전자간 골절에서 불안정 골절은 사회활동이 적고 골밀도 T-score -3.0 이하인 경우 많음을 알수 있었다. Purpose : We studied the co-relation on the causes of the hip fracture through the analysis of a relevance on the etiological factors as increased incidence according increasing old age. Materials and Methods : Total 158 cases that treated on the hip fracture from 2001 Jan. to 2002 May were studied. The parameters were age, gender, smoking, obesity, type of fracture, place of fracture, other comorbidity, activity of daily living, bone marrow densitometry, hardness of floor, orientation, injury energy. And then, we analysis of difference between femur neck fracture and femur intretrochanter fracture and between stable femur intertrochanter fracture and unstable femur intertrochanter fracture. Results : The incidence of the femoral intertrochanteric fracture was larger significantly than that of the femoral neck fracture in the older then 80(p<0.001). On the comparison of the pre-fractural activity of daily living, the group revealed lower activity had larger incidence of femoral intertrochateric fracture(p<0.001). Also, the femoral intertrochanteric fracture was more larger osteoporosis patient group(p<0.005), and lower energy trauma(p<0.05). In a unstable femoral intertrochanteric fracture, 21 cases(77.7%) of total 27 cases were belong to the group of the activity of daily living scale below C(p<0.05), 24 cases pf 27 cases were belong to the osteoporosis patient who was estimated below -3.0 on T-score(p<0.001). Conclusion : In the hip fracture of the elderly patients, the femoral intertrochanteric fracture is more prevalance ratherthe femoral neck fracture on the cases of older patient more than 80 year-old, lower activity of daily living scale, lower T-score less than -3.0 on BMD, lower energy trauma. Also, in femur intertrochanter fracture, unstable fracture is more common in low daily activity and owteoporosis.

국내 설사환자로부터 분리된 Escherichia coli 균의 혈청군 및 Verocytotoxin 생성능

김호훈,강연호,김성한,박미선,유재연,이복권 대한감염학회 1998 감염 Vol.30 No.5

목적: 국내 설사 환자에게서 분리 동정된 인체 감염 유래 E. coli 균의 혈청군, verocytotoxin 생성능을 구명하여 EHEC 균의 인체 감염 실태를 구명하고, 동물 및 식품 유래 분리균주의 성상과 비교 검토하여 인체 감염 위험성에 대한 기초적 자료를 의학계에 제공하고자 하였다. 방법: EHEC 감염의심 설사 환자 분변 검체로부터 분리된 균주 중 생화확적 성상에 따라 대장균을 분리하였고 이들 균주 중 E. coli O157:H7를 분리하기 위해서는 Dsorbitol 음성균을 선별하였으며, 분리균에 대하여 항혈청으로 응집 시험을 실시하여 혈청군을 구명하였다. 혈청군이 확인된 균주에 대하여 중합효소 연쇄반응 (PCR) 및 역수동라텍스 응집시험 (Reversed Passive Latex Agglutination:RPLA) 으로 verocytotoxin 생성능을 구명하였다. 결과: 장출혈성 대장균 감염 의심설사 환자의 분변검체로부터 25주의 E. coli 균을 분리 동정하였으나 이들 균주는 모두 verocytotoxin을 생성하지 않았다. 분리 동정된 대장균으로부터 확인된 혈청군은 16종으로서 E. coli O1, O6, O8, O15, O20, O25 O26, O28, O29, O44, O86a, O119, O126, O128, O152, 및 O157:H-였으며 E. coli O157:H-혈청형 균주와 E.coli O25 혈청군 균주가 각각 3주씩 분리되어 비교적 높은 분리율을 보였다. 결론: 장출혈성 대장균 감염 의심설사 환자 분변 가검물로부터 총 25주의 E. coli균을 분리 동정하였으나 verocytotoxin을 모두 생산하지 않았다. Background: Since 1982, many countries has reported outbreaks or sporadic cases caused by enterohaemorrhagic Escherichia coli (EHEC) serogroup strains, mainly E. coli O157:H7 type strain. However, systemic investigation about EHEC agents, including E. coli O157:H7, have not been done in Korea. Therefore, we investigated serogroup and verotoxin productivity of E. coli strains isolated from diarrheal patients and estimated risk of human infection in comparison with EHEC strains isolated from cow, pig, and food material in Korea. Methods: Diarrheal patient stool samples were collected and E. coli strains were isolated, according to biochemical characteristics. In order to isolate E. coli O157:H7, D-Sorbitol negative strains were selected. Serogrouping of the E. coli isolates was done by agglutination test. Verocytotoxin productivity was investigated by polymerase chain reaction (PCR) and reversed passive latex agglutination (RPLA). Human infection risk was estimated in comparison with EHEC strains isolated from cow, pig and food materials in Korea. Results: Twenty-five E. coli strains were isolated from the diarrheal patients who were suspected to be infected with EHEC. However, none of these E. coli strains produced verocytotoxin. Out of 25 E. coli isolates, 16 serogroups of E. coli O1, O6, O8, O15, O20, O25, O26, O28, O29, O44, O86a, O119, O126, O128, O152 and 157:H-were found. In each of the E. coli O157:H-and O25 serogroups 3 strains were found. Conclusion: None of 25 E. coli isolated from diarrheal patients who were suspected of EHEC infection produced verocytotoxin producing E. coli have been reported recently in Korea.