생체분해성 망막압정을 이용한 망막고정에 대한 실험적 연구

김용백,민병무,김창식,박근성,김승영,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Biodegradable retinal fixation devices obtain mechnical fixation of the retina with desirable chorioretinal scarring and with the potential for local, sustained release of antimetabolites and steroids to inhibit proliferative vitreoretinopathy. We manufactured a biodegradable retinal tack with barb that was designed in order to prevent intrusion from implantation of retinal tacks. This study was carried to evaluate the efficacy for retinal fixation and the capability for sustained release of drugs with a newly designed biodegradable retinal tack Biodegradable retinal tacks were made of polymers of glycolic acids and were designed with barbs in a shape to prevent the disinsertion. Biodegradale retinal tacks are divided into 3 parts, a conical portion that is inserted into the sclera, a cylinder portion that remains in the vitreous, and a neck portion between the pin and the cylinder. The tapered conical end was manufactured to allow easy insertion through the retina and choroid into the sclera. A cylinder portion was manufactured with a tapered angle that fixes firmly into the orifice of 19 gauge spinal needle. A neck portion, 0.4 mm in diameter, was designed to prevent disinsertion from following implantation of retinal tack. The applicator was a 19 gauge spinal needle and its orifice was prepared to 15°angle to accept the tapered cylinder portion of the retinal tack. The retinal tacks, secured in the needles, were passed through the formed vitreous and inserted into the retina, choroid, and sclera and were released by pushing the internal needle, usually within 2-3mm of the medullary ray of the posterior rabbit retina A retinal tack was placed in each of 8 pigmented rabbit eyes. Slit-lamp biomicroscopy, indirect ophthalmoscopy and fundus photography were performed periodically from 1 day to 8 weeks after surgery. Eight eyes were enucleated and studied by light microscopy at 8 weeks. Biomicroscopic evaluation of the animals revealed edemas adjacent to the retinal surfaces immediately after insertion of the biodegradable retinal tacks in all the animals. These edemas disappeared after 1 week. The first noticeable change in the size of retinal tacks was shown after 2weeks. The size of the retinal tacks gradually got smaller, decreasing to about one-half at 4 weeks and about one-third at 8 weeks. All retinal tacks remained in inserted places without any movement for an 8 week period. On light microscopy, epiretinal proliferations were seen to extend into the vitreous cavity. Cellular capsules that lined the inner aspect of the scleral defect caused by tack insertion were found. However the adjacent retina had a normal cytologic appearance and architecture in all specimens. We manufactured a biodegradable retinal tack that is designed to prevent intrusion from implantation of retinal tacks. All biodegradable retinal tacks reduce in size with time, but no retinal tacks extruded from the inserted place. The newly designed biodegradable retinal tack can be used for retinal fixation and may be used as a vehicle for the introduction of pharmacologic agents to prevent the cellular events that promote proliferative vitreoretinopathy.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

호랑나비 유래 항균 펩타이드 파필리오신 3의 항염증 활성

신용표(Yong Pyo Shin),이준하(Joon Ha Lee),김인우(In-Woo Kim),서민철(Minchul Seo),김미애(Mi-Ae Kim),이화정(Hwa Jeong Lee),백민희(Minhee Baek),김성현(Seong Hyun Kim),황재삼(Jae Sam Hwang) 한국생명과학회 2020 생명과학회지 Vol.30 No.10

본 연구에서는 호랑나비 유충의 유전체 분석을 통해 선별된 파필리오신 3의 항균 및 항염증 활성을 확인하였다. 선행연구에서 RNA 시퀀싱 분석을 통해 호랑나비의 전사체를 분석하였으며, 결과를 바탕으로 인실리코(in silico)분석을 진행하여 전사체 유래 항균 펩타이드를 스크리닝하고 선발하였다. 수행된 항균 활성 및 용혈 활성 테스트에서 파필리오신 3은 그람음성균인 E. coli와 그람양성균인 S. aureus에 대해 강력한 항균활성을 나타낸 반면 마우스 적혈구에 대한 용혈 활성은 전혀 없었다. 다음으로 마우스 대식세포주 Raw264.7 세포를 이용하여 파필리오신 3의 항염증 활성을 확인하였다. 그 결과 파필리오신 3은 LPS로부터 유도된 Raw264.7 세포들의 산화질소 생성을 감소시키는 결과를 보여주었다. 뿐만 아니라 실시간 역전사 중합효소 연쇄반응(qRT-PCR) 방법과 효소결합 면역흡착측정법(ELISA)을 통해 파필리오신 3이 Raw264.7 세포에서 전염증성 사이토카인(IL-6, IL-1β)의 발현을 감소시킨다는 것을 확인할 수 있었다. 또한, 염증반응의 신호전달인자들(MAPKs, NF-κB)의 인산화를 억제하는것을 확인하였는데, 이는 파필리오신 3이 LPS와의 상호작용을 통해 결합하여 효과를 나타낸다는 것을 확인할 수 있었다. 이러한 결과들은 호랑나비 유전체 분석을 통해 확인된 파필리오신 3이 새로운 항균 및 항염증 치료제로서 개발하는데 가능성 있는 물질로 사료된다. The development of novel peptide antibiotics with potent antimicrobial activity and anti-inflammatory activity is urgently needed. In a previous work, we performed an in-silico analysis of the Papilio xuthus transcriptome to identify putative antimicrobial peptides and identified several candidates. In this study, we investigated the antibacterial and anti-inflammatory activities of papiliocin 3, which was selected bioinformatically based on its physicochemical properties against bacteria and mouse macrophage Raw264.7 cells. Papiliocin 3 showed antibacterial activities against E. coli and S. aureus without inducing hemolysis and decreased the nitric oxide production of the lipopolysaccharide-induced Raw264.7 cells. Moreover, ELISA and Western blot analysis revealed that papiliocin 3 reduced the expression levels of pro-inflammatory enzymes, such as inducible nitric oxide synthase (iNOS), cyclooxygenase- 2 (COX-2), and prostaglandin E2 (PGE2). In addition, we examined whether papiliocin 3 could inhibit the expression of pro-inflammatory cytokines (interleukin-6 and interleukin-1β) in LPSinduced Raw264.7 cells. We found that papiliocin 3 markedly reduced the expression level of cytokines through the regulation of mitogen-activated protein kinases (MAPK) and nuclear factor kappa B (NF-κB) signaling. We also confirmed that papiliocin 3 binds to bacterial cell membranes via a specific interaction with lipopolysaccharides. Collectively, these findings suggest that papiliocin 3 could be a promising molecule for development as a novel peptide antibiotic.

케이블 돔 구조물의 축대칭 하중에 의한 비선형 불안정 현상에 관한 연구

백인성,김형석,김숭덕 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

One of important problem, in large space structure, is to overcome the self weight of roof construction. This problem can be solved with using tension members effectively. Thus the rapid progress of Hybrid structure, that makes effective use of the means of settling, has a good effect on realizing the large space. In this study, the shape finding problem by applying initial stresses is investigated and using this we examined the unstable phenomena of the perfectly shaped and the models have initial imperfection under axisymmetric load to grasp the unstable behaviour of the Geiger and Flower type in the cable domes.

Ethyl α-cyano-β-phenylacrylate 유도체의 가수 분해 메카니즘과 반응속도론적 연구

許泰聖,白仁淑 聖心女子大學校 1985 論文集 Vol.17 No.-

여러 pH에서 ethyl α-cyano-β-phenylacrylate유도체(H, p-Cl, p-CH₃, p-CH₃O, p-NO₂)의 가수분해 속도상수을 측정하여 넓은 pH범위에서 잘맞는 속도식을 구하고 이것으로부터 전 pH범위에서 적용되는 반응속도식을 구하였다. 이 속도식으로부터 실험 사실에 잘 맞는 반응메카니즘을 제안 하였다. pH7이상에서는 hydroxide 이온이 pH7이하의 산성용액에서는 물분자가 직접 참가되는 것으로 생각된다. The rate constants of the hydrolysis of the derivatives of ethyl α-cyano-β-phenylacrylate(H, p-Cl, p-CH₃, p-CH₃O, p-NO₂) were determined at various pH. The rate equation can be applied over a wide pH range were obtained. From the rate equation and experimental datas. one can conclude that above pH7 the hydrolysis of ethyl α-cyano-β-phenylacrylate derivatives are initiated by the attack of hydroxide ion, however below pH7, started by the addition of a water molecule.

국내 식물자원 추출물에 대한 항산화 활성 검색

오성준,백남인,김해영 경희대학교 생명자원과학연구원 1999 硏究論文集 Vol.20 No.-

We investigated the antioxidative activity to obtain novel antioxidants from plant resources in Korea. Three hundreds species of plants were collected and screened with DPPH (1.1-diphenyl-2-picryl hydrazyl) radical test. From this work. 10 species of plants showed a significant antioxidative activity.

매립가스 및 폐플라스틱의 친환경 분해를 통한 수소 · 탄소 활용 네트워크 구축

한효림, 성인호, 서원덕, 조은정, 백주은, 송태진 충북대학교 건설기술연구소 2023 충북대학교 건설기술연구소 논문집 Vol.42 No.2

본문참고

Fragmentectomy versus Conventional Microdiscectomy in Single-Level Lumbar Disc Herniations : Comparison of Clinical Results and Recurrence Rates

Baek, Geum-Seong,Kim, Yeon-Seong,Lee, Min-Cheol,Song, Jae-Wook,Kim, Sang-Kyu,Kim, In-Hwan The Korean Neurosurgical Society 2012 Journal of Korean neurosurgical society Vol.52 No.3

Objective : This retrospective study aimed to compare clinical outcomes in terms of pain relief and recurrence rate between fragmentectomies and conventional microdiscectomies in patients with lumbar disc herniation (LDH). Methods : Between January 2008 and May 2011, a total of 175 patients met the inclusion criteria of this study. The visual analogue scale (VAS) scores of back and radicular pains were recorded before surgery, 2 and 6 weeks after surgery. Recurrence was defined when a patient had the same pattern of preoperative symptoms and was confirmed with magnetic resonance imaging. Results : Seventy-four patients (42.3%) were suitable for fragmentectomy, and 101 patients underwent conventional microdiscectomy. There were no significant differences in VAS scores between the fragmentectomy and conventional microdiscectomy groups 2 and 6 weeks after surgery. During the follow-up period, 3 patients (4.05%) in the fragmentectomy group and 7 patients (6.93%) in the conventional microdiscectomy group relapsed. Conclusion : If patients are selected according to well-defined criteria, fragmentectomy can be a good surgical option for LDH, in the physiological aspect of preserving healthy intervertebral disc materials.

<i>In Vivo</i> Study of Natural Killer (NK) Cell Cytotoxicity Against Cholangiocarcinoma in a Nude Mouse Model

JUNG, IN HYE,KIM, DO HEE,YOO, DA KYUNG,BAEK, SUN YOUNG,JEONG, SEONG HOON,JUNG, DAWOON E.,PARK, SEUNG WOO,CHUNG, YONG-YOON ANTICANCER RESEARCH 2018 IN VIVO -ATHENS- Vol.32 No.4

<P>Background/Aim: Natural killer (NK) cells are one of the lymphocytes clinically used for various cancer types. Cytotoxicity of NK cells to cholangiocarcinoma (CC), however, has not yet been studied. Nor NK cell therapy against CC has been clinically applied. In this study, relevance of NK cell therapy for anti-tumor efficacy against CC was pre-clinically investigated. Materials and Methods: Human HuCCT-1 cells, an intrahepatic CC cell line, were xenografted into nude mice. The HuCCT-1 tumor-bearing nude mice then received multiple infusions of ex vivo-expanded human NK cells (SMT01) and in vivo cytotoxic activity of the NK cells against the CC cells was evaluated. Results: SMT01 infusion resulted in significant inhibition of the CC tumor growth. Body weight of the mice administrated with chemotherapy was found to be maintained at the lowest level among all treatment groups while all the SMT01 infusion groups well maintained their body weight. Conclusion: The present in vivo study demonstrates that NK cells contain cytolytic activity against cholangiocarcinoma and show beneficial effect of NK cell therapy in relevance to quality of life. Further investigation of the NK cell-based immunotherapy can be useful to determine cancer therapeutics for the specific tumor.</P>