Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

학교상담 수퍼비전 모형 구안

손현동,강진령 이화여자대학교 교육과학연구소 2007 교육과학연구 Vol.38 No.2

전문상담교사의 전문성 신장과 효율적인 업무 수행을 돕기 위해 학교상담의 특성과 전문상담교사들의 독특한 역할을 고려한 학교상담 수퍼비전 모형이 구안되어야 할 필요가 있다. 이 연구의 목적은 전문상담교사를 위한 학교상담 수퍼비전 모형을 구안하는 것이다. 이러한 연구 목적을 달성하기 위해 수퍼비전 모형에 대한 문헌고찰을 통해 학교상담 수퍼비전 모형 구안의 방향을 설정하고 이를 바탕으로 모형을 구안하였다. 구안된 학교상담 수퍼비전 모형의 구성은 전문상담교사의 수퍼비전 목표, 수퍼비전 과정, 수퍼바이저의 역할·양식, 수퍼비전 방식을 포함하는 것이며 발달단계에 따라 개입방법이 선택되어지는 것으로 이루어져있다. 이 모형은 발달모형에 근거하고 있다는 점과 수퍼비전 목적과 목표, 수퍼비전 과정, 개입방법을 모두 포함하고 있는 통합적인 모형이라는 점이 특징이다. 구안된 학교상담 수퍼비전 모형은 학교상담의 특성과 전문상담교사들의 역할을 고려한 것으로, 전문상담교사들의 전문성 향상과 발달을 돕고, 효율적인 업무수행을 도울 수 있을 것으로 기대된다. To help school counselors enhance expertise and deliver a service effectivelyit is necessary to construct a school counseling supervision model reflecting the characteristics of school counseling and the peculiar roles of school counselors. The purpose of this study was to construct a school counseling supervisionmodel for school counselors. To achieve this purpose, the principles ofconstructing a model was established by reviewing literature about counselingand school counseling supervision model. And then based on these principles, aschool counseling supervision model was constructed. The school counseling supervision model presented in this study consists offour phases(entry, preparatory, implemental, terminal), and each phases hassubordinate stages. The constructed school counseling supervision model hascharacteristics that it is grounded on the developmental models and containssupervision purpose and objects, process, and intervention methods. Theconstructed supervision model is expected to help school counselors' professionalimprovement and their personal growth.

학교상담 수퍼비전 모평 구안

손현동,강진령 이화여자대학교 사범대학 교육과학연구소 2007 교육과학연구 Vol.38 No.2

전문상담교사의 전문성 신장과 효율적인 업무 수행을 돕기 위해 학교상담의 특성과 전문상담교사들의 독특한 역할을 고려한 학교상담 수퍼비전 모형이 구안되어야 할 필요가 있다. 이 연구의 목적은 전문상담교사를 위한 학교상담 수퍼비전 모형을 구안하는 것이다. 이러한 연구 목적을 달성하기 위해 수퍼비전 모형에 대한 문헌고찰을 통해 학교상담 수퍼비전 모형 구안의 방향을 설정하고 이를 바탕으로 모형을 구안하였다. 구안된 학교상담 수퍼비전 모형의 구성은 전문상담교사의 수퍼비전 목표, 수퍼비전 과정, 수퍼바이저의 역할·양식, 수퍼비전 방식을 포함하는 것이며 발달단계에 따라 개입방법이 선택되어지는 것으로 이루어져있다. 이 모형은 발달모형에 근거하고 있다는 점과 수퍼비전 목적과 목표, 수퍼비전 과정, 개입방법을 모두 포함하고 있는 통합적인 모형이라는 점이 특징이다. 구안된 학교상담 수퍼비전 모형은 학교상담의 특성과 전문상담교사들의 역할을 고려한 것으로, 전문상담교사들의 전문성 향상과 발달을 돕고, 효율적인 업무수행을 도울 수 있을 것으로 기대된다. To help school counselors enhance expertise and deliver a service effectivelyit is necessary to construct a school counseling supervision model reflecting the characteristics of school counseling and the peculiar roles of school counselors. The purpose of this study was to construct a school counseling supervisionmodel for school counselors. To achieve this purpose, the principles ofconstructing a model was established by reviewing literature about counselingand school counseling supervision model. And then based on these principles, aschool counseling supervision model was constructed. The school counseling supervision model presented in this study consists offour phases(entry, preparatory, implemental, terminal), and each phases hassubordinate stages. The constructed school counseling supervision model hascharacteristics that it is grounded on the developmental models and containssupervision purpose and objects, process, and intervention methods. Theconstructed supervision model is expected to help school counselors' professionalimprovement and their personal growth.

일개대학병원에서 수막알균 백신 사용의 경험

손동욱,이찬우,정영국,조래정,이혜경,김은실,이진수,정문현 대한감염학회 2007 감염과 화학요법 Vol.39 No.6

Meningococcal infection is a life threatening disease that leaves serious sequelae in spite of appropriate treatment, thus vaccination for high risk groups are Important for the prevention of meningococcal diseases. However, the vaccine for Neisseria meningitidis has not been available in Korea until we introduced bivalent (serogroup A and C) polysaccharide vaccine for the first time for relief works in our university hospital. The vaccine was administered from January 2005 to March 2007 to 317 persons. of the groups administered, the largest group among them were 133 (133/317, 42%) students who planned to study abroad and needed the vaccination for secure entrance to school dormitories. This group was followed by health care workers, travellers to the regions of the world with high risks of meningococcal diseases, and splenectomised patients. To ratioalize the domestic use of meningococcal vaccine, the availability of vaccines first needs to be simplified by introducing them to the domestic market; for this to be possible, the approval system for vaccines should be reformed and epidemiogical studies need to be carried out.

미량 Ni 첨가 Fe-Ni 합금의 고온산화 거동에 관한 연구

孫根洙,尹在弘,金晋夏,金顯洙,成田敏夫,林重成 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.10

Oxidation behavior of Fe-Ni alloys added by small amount of Ni was studied, under the exposure to the temperatures up to 1100℃, 1150℃, and 1200℃, respectively. The slope of mass change curve representing an oxidation rate was 1 or 2 up to 20 seconds and decreased to 1/2 after 20 seconds in a parabolic shape. The temperature of the initial stage of oxidation increased more quickly at the furnace inside than at the surface of the specimen, and its behavior was reversed due to oxidation heat after 20 seconds later. Oxide layer was mainly composed of FeO. And particle of Fe_3O_4 and Fe_2O_3 was distributed in FeO layer. The order of the composition ratio for the oxide was Fe_2O_3,·Fe_3O_4,·FeO at the oxide surface, FeO,·Fe_3O_4,·Fe_2O_3 at the internal oxide, and FeO,·Fe_3O_4,·Fe_2O_3 at the surface of metal after stripping oxide layer.

靑少年體操의 運動强度 및 에너지 消費量에 관한 硏究

孫泰烈,趙顯喆,鄭正眞 成均館大學校 科學技術硏究所 1988 論文集 Vol.39 No.1

The purpose of this study is to analysis energy expenditure and exercise intensity in adolesent gymnastics. The regression equation obtained from the results of HR and VO_2 was used to calculate Energy consumption for 7males and 5females. As a result of this study it was gained the conclusion as following 1. The energy expenditure in adolesent gymnastics appeared 36.26±7.80cal/4.5min, 19.17±3.85ca1/4.5min male and female students for each. 2. From the view of %HR_max, the exercise intensity of the adolesent gym. appeared 74.2±9.6%, 85.0±13% male and female students for each. 3. It was concluded that the adolesent gym. would be an adequate exercise in problem having training and warm-up effect to male student, training effect to female student.

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

Effects of Pilger Rolling and Heat Treatment on Microstructure and Corrosion Resistance of Ag-electroplated 304 Stainless Steel Tubes

Hyun Park,Woo‑Jin Lee,Jae‑Han Son,Han‑Kyun Shin,Sung‑Kyu Hong,Hyo‑Jong Lee 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

We introduce a cost-effective method that combines electroplating with metal cold working processes to manufacture compositetubes with robust corrosion resistance. First, a 3-step electroplating process was developed to form an adhesive anduniform Ag coating on the outer wall of a 304 stainless steel tube. The process consisted of a Ni-strike step for removing thepassivation layer of the initial 304 tube, Cu deposition for smoothing the surface by adding a buffer layer, and Ag deposition. To reinforce the interfacial adhesion between the electroplated layers and the 304 tube and increase the area of the Ag coating,a pilger rolling or pilger rolling + heat-treatment process was performed after the Ag electroplating process. Scanningelectron microscopy of the composite tubes after each process indicated that the as-deposited Ag coating changed fromhaving a rough to smooth surface after only pilger rolling and with additional heat treatment. Electron backscatter diffractionanalysis of the microstructures and textures of the tubes revealed that dynamic recrystallization occurred extensively duringpilger rolling, resulting in the formation of a relatively defect-free grain structure without heat treatment. Furthermore,electrochemical polarization curves determined that the Ag-coated composite tubes are superior to the uncoated 304 tubein terms of corrosion resistance under Cl−atmosphere, owing to the formation of a AgCl passivation layer during testing. Surface analysis of the composite tubes suggests that the properties of the AgCl passivation layer are related to the grainsize of the Ag coating and the density of plastic-deformation-induced defects.

뇌동맥류파열에 의한 뇌지주막하출혈 환자의 수술시기에 따른 예후 분석

손진열,박인성,황수현,백선하,김은상,정진명,김재형,한종우 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.3

석면관련 폐질환의 진단에 있어서 흉부고해상도전산화단층촬영검사(HRCT)의 유용성

이현재,손지언,,홍영습,,이영일,예병진,유창훈,정갑열,김상훈,임형준,장은철,김정만,김준연 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 석면에 노출된 근로자에서 석면관련 폐질환의 양상을 분석하고 진단에 있어 HRCT의 유용성을 연구하여 석면에 대한 의학적 감시프로그램의 개발에 기여하고자 하였다. 방법: 석면에 직접 노출되는 작업자 68명과 간접 노출되는 작업자 94명 등 총 162명에 대해 OSHA 석면 표준, 의학적 감시 프로그램에 따라 단순 흉부방사선검사, 폐활량검사 및 우리나라 실정에 맞게 일부 수정한 의학적 설문지를 통해 면접조사의 방식으로 환자에 관한 정보를 수집하였으며, 자료의 신뢰성을 확보하기 위하여 면접조사는 1달 이상의 기간을 두고 2차례에 걸쳐 실시된 후 확인, 수정하였다. 또한, 석면관련 폐질환을 확진하기 위하여 HRCT를 추가적으로 실시하였다. 결과: HRCT결과 석면관련 폐질환의 유병율은 162명의 근로자중 17명으로 10.5%였는데, 직접 노출군에서 10명 (14.7%), 간접 노출군에서 7명 (7.4%)이었다. HRCT와 비교할 때 단순 흉부방사선검사의 민감도와 특이도는 각각 70.6%와 98.6%이었고, 양성 예측도와 음성 예측도는 각각 85.7%와 96.6%으로, HRCT는 단순흉부방사선검사에 비해 민감도가 높아 초기 석면관련 폐질환을 진단하는데 효과적이었다. 석면관련 폐질환의 진단에 있어 HRCT와 폐활량검사 사이에 유의한 관련성이 관찰되었다. HRCT 결과 석면관련 폐질환의 유무와 의학적 설문조사 결과의 빈도분석을 실시한 결과, 유의한 변수는 작업기간, 흡연량, 호흡기질환의 과거력, 기침, 호흡곤란이었다. 결론: HRCT는 석면관련 폐질환의 진단에 있어 단순 흉부방사선검사와 비교하여 민감도가 높아 초기 진단에 유용하므로 단순 흉부방사선선검사 결과 석면관련 폐질환소견을 보일 때뿐만 아니라, 정상소견을 보이더라도 폐활량 검사결과와 근무력, 흡연력, 호흡기질환의 과거력, 기침, 호흡곤란 등의 호흡기 증상을 참고하여 HRCT검사를 고려해야 한다. 또한, 본 연구에서 관련성이 없었던 요인들에 대해서도 향후 연관성을 규명하는 광범위한 전향적 연구가 필요하다. Objectives: This study was carried out to improve the medical surveillance program of workers exposed to asbestos by examining the usefulness of High Resolution Computed Tomography (HRCT) in the diagnosis of asbestos-related lung disease. Methods: The study subjects comprised 162 workers in a ship-repairing yard, 68 of whom had been directly exposed to asbestos and 94 indirectly exposed. The 'Occupational Safety & Health Administration (OSHA) asbestos standard, medical surveilance program' and HRCT were conducted to analyze the aspects of the asbestos-related lung disease. The OSHA asbestos standard, medical surveilance program consists of simple chest x-ray, spirometry and medical questionnarie. Results: Seventeen (10.5%) of the 162 subjects, 10 (14.7%) directly exposed and 7 (7.4%) indirectly exposed, revealed asbestos-related lung disease on HRCT. The sensitivity and specificity of simple chest x-ray for asbestos-related lung disease were 70.6% and 98.6%, and the positive and negative predictive values were 85.7% and 96.6% respectively, as compared with HRCT, HRCT was an effective diagnostic tool especially to detect early asbestos-related lung disease. The study results indicated a relative significant association between the results of spirometry and HRCT. The variables significantly associated with asbestos-related lung disease on HRCT were work duration, smoking history (pack-years), past history of respiratory disease, cough and dyspnea. Conclusions: In the diagnosis of asbestos-related lung disease, HRCT should be considered not only for workers with positive findings on simple chest x-ray, but also workers with specific findings on spirometry, occupational history, smoking history, and past history of respiratory disease, or with respiratory symptoms such as cough and dyspnea.