중학교 화학 분야의 개념 연구에 대한 문제점 분석

朴恩姬,姜대勳,白盛惠,朴國泰,金惠敬,蔡禹기,權鈞 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

중학교 화학 분야의 오개념 조사에 사용된 설문지의 문제점을 밝혀, 학생들의 정확한 오개념 조사를 위한 바탕을 마련하고자 한다. 기존의 중학교 화학 분야에 대한 오개념 조사 설문지가 연구 대상 학생들에게 잘못 사용되고 있었으며, 문항 내용 자체에도 오류가 있었음을 지적하고자 하는 것이다. 선행 연구들의 분석 결과들이 오개념이 아니라 무개념임을 확인하기 위하여 중학교 1학년 학생 182명을 대상으로 두 종류의 설문지 A와 B를 투입하여 조사하였다. 설문지 A는 오개념 연구를 위해 선행 연구들에서 사용한 문항들로 이루어진 것으로, 이를 투입한 결과를 선행 연구들의 결과들과 비교하기 위한 것이다. 그리고 설문지 B는 설문지 A에서 응답한 학생들의 구체적인 생각을 알아보기 위하여 새롭게 개발한 것이다. 설문지 A를 통해 얻은 결과와 선행 연구들에서 얻은 결과들을 비교할 때, 전반적으로 일관성이 없게 나타났으며, 중학교 1학년 학생들의 주관식 정답률이 객관식 정답률에 비해 매우 낮게 나타나는 공통점은 있었으나, 문항 자체의 오류들이 발견되었다. 설문지 B를 통해 학생들의 생각을 알아본 결과, 설문지 A에서 과학적 개념과 오개념으로 분류된 학생들 중에서 무개념으로 판단된 경우가 많았다. This study was to clarify the problems of questionnaires related to misconception researches of middle school chemistry and to prepare a ground for the correct misconception research for students. For these purposes, the questionnaires of the misconception researches related to chemistry in middle school were analyzed, and the mistakes of the questions which were used in 7th grade students were found, based on a definition of misconceptions in this study. Also, the errors in contents of the questionnaires were indicated. The results of the analysis revealed that most of the 7th grade students did not have misconceptions contrary to some of the previous researches conclusions. They only had no conceptions related to the contents. In order to support this analysis, two different questionnaire sets (questionnaire set A and B) for 182 7th grade students were used. After obtaining similar results to those of the previous researches by the questionnaire set A, the students' thoughts were examined by the questionnaire set B. From the results of this study, the correct answer rates of subjective questions were very low compared with those of objective questions in the questionnaire set A for 7th grade students. It was hard to find consistency among the results of the researches using the questionnaire set A. And many contents of the questions consisted of the questionnaire set A were not consisted with a definition of misconceptions. There were errors in the contents of the questions, too. The students classified as the having-science-concepts group and the having-misconceptions group by the questionnaire set A were classified as the having-no-concepts group by the questionnaire set B.

퍼팅 동작 영상분석 시 프레스 동작의 이벤트 설정 가능성 여부

박태진,서국웅,서국은 釜山大學校 附設 體育科學硏究所 2009 體育科學硏究所 論文集 Vol.25 No.1

본 연구는 부산.경남지역에서 세미프로로 활동 중인 7명을 선정하여 퍼팅(10.94 m)동작 시 프레스 동작을 3차원 영상 기법을 통해 운동학적으로 비교.분석하여 얻은 결론은 다음과 같다. 첫 번째, 양손은 어드레스를 취한 후 백스윙되기 전까지 볼 진행 방향으로 움직이는 것으로 나타났다. 두 번째, 퍼터헤드, 몸통, 어깨 중심점은 프레스 동작 시 좌.우 방향으로 움직이지 않고 고정되어 있는 것으로 나타났으며, 프레스 시 양손 중심점은 어드레스 시 보다 임팩트 시에 가까운 것으로 나타났다. 세 번째, 퍼팅 스트로크 시 총소요시간 중 프레스 동작이 22.83%를 차지하고, 구간 중 다운스윙과 팔로스루 시간 보다 많이 소요되는 것으로 나타났다.

韓國人에서 多重增幅 重合酵素反應으로 分析한 STRs 遺傳座位의 遺傳的 多樣性

권국환,이혜린,박종진,황적준,이희석,송은섭 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

The tetrameric STRs loci were studied in a population of Korean(n=223) for allele frequency distribution and applicability to identity and paternity testing, using multiplex PCR with electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by silver staining. The STRs loci analyzed were HUMCSF1PO, HUMTPOX, and HUMTHO1 ; in the Korean population, 9 alleles with their frequency range of 0.002-0.363 are detected in the HUMCSF1PO, 5 alleles with those of 0.020-1.480 in the HUMTPOX, and 6 alleles with those of 0.002-0.489 in the HUMTHO1. The highest observed heterozygosity is found at the locus HUMCSF1PO(0.709), those of the loci HUMTPOX and HUMTHO1 being 0.646 and 0.632, respectively. All loci meet Hardy-Weinberg expectations ; there are good agreement between the observed and expected values under Hardy-Weinberg equilibrium. Moreover, pairwise comparisons between loci show allelic independence for all the 3 loci comparisons. The power of discrimination (PD) determined for the locus HUMCSF1PO is 0.8896 (88.96%), that of the HUMTPOX is 0.8818(88.18%), and that for the HUMTHO1 is 0.8367(83.67%) ; the combined power of discrimination for the triplex is 0.9976(99.76%). The power of exclusion(PEX) calculated for the loci HUMCSF1PO, HUMTPOX, and HUMTHO1, being prior probability that a falsely accused father will be excluded, and 0.5173(51.73%), 0.4610(46.10%), and 0.5101(510.01%), respectively, and the cummulative power of exclusion(CPE) for all the three loci is 0.8726(87.26%). Thus, these allelic frequency data can be used to construct the database of the multiplex PCR-based DNA profile in the Korean population. The calculated parameter, "power of discrimination(PD)" and "power of exclusion(PEX)", show the informativeness of these loci for the determination of identity and relatedness of individuals.

人蔘이 卵巢摘出로 誘發된 흰쥐의 骨多孔症 예방에 미치는 효능

정국영,서부일,이은숙,변부형,신순식,박지하 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objectives : The present study has been undertaken to investigate the effects of Ginseng Radix on prevention of osteoporosis in ovariectomized rats. Methods : In this experiment, the rats were ovariectomized. Rats were administered by 2 kinds of medicine, Estradiol, Ginseng Radix. The levels of bone mineral density, osteocalcin, ALP, phosphorus, calcium in serum, deoxypyridinoline in urine, calcium, phosphorus, ash weight of bone, body weight and uterus weight were measured. Results : The levels of spinal bone mineral density was significantly increased in comparison with OVX group at 8 weeks in Ginseng Radix group. The levels of serum didn t show significant change in comparison with OVX group in Ginseng Radix group. The levels of urine deoxypyridinoline showed significant decrease in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial ash weight didn t show significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial calcium showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial phosphorus showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of body weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. The levels of uterus weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. Conclusions : Reviewing these experimetal results, it appears that Ginseng Radix have efficacy on prevention of osteoporosis. And further study should be conducted to illustrate in depth the curing and prevention of osteoprosis.

유방암과 관련된 부수종양성 Stiff-Person Syndrome 1예

방현,이상암,최은정,김광국,박성혜 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

한국형 후각검사 KVSS Test의 개발

홍석찬,유영석,김은서,김석천,박수홍,김진국,강성호 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

Investigation of the Potential of Modified Handmade Cloning (mHMC) Assisted with Demecolcine (DEM) in Porcine Embryo Cloning

Eun Ji Lee,Kuk Bin Ji,Kang Sun Park,Kyeong Yeob Kim,Beom Sik Kim,Kyu hyun Kim,Ryeong Eun Kim,Eun Young Kim,Ji Hye Lee,Ju Lan Chun,Min Kyu Kim 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06

Somatic cell nuclear transfer(SCNT) using micromanipulator have been used as a traditional cloning technique and applied various researches during the last decades. However the micromanipulator is expensive and relatively longer training period is required to operate it efficiently. Handmade cloning(HMC) is an alternative cloning method in a simplified way compared to the traditional cloning. Here, we suggested modified porcine handmade cloning(mHMC) as a new approach to clone porcine embryos as a substitute of the traditional SCNT. In mHMC, a nucleus was removed by an aspiration method by using a glass pipette, instead of bisection method. In this study, we investigated the efficiency of the enucleation method in mHMC by assessing the developmental competence of embryos in comparison with the traditional SCNT. The efficiency of enucleation was evaluated based on the rate of the accuracy and oocyte survivability. The accuracy of enucleation was lower in mHMC compared to those in SCNT(98.01±0.57 vs. 83.83±2.47), and the rate of survived oocytes was also lower in mHMC(96.50±0.84 vs. 90.10±2.11, respectively). And the developmental competence was assessed. The blastocyst rate was significantly higher in mHMC group(13.53±2.08 vs. 20.48±0.99). The levels of apoptosis and ROS were investigated to evaluate embryo quality. The expression of ROS and apoptosis-related genes showed no difference between groups. And the relative expressions of mRNA of pluripotency genes and reprogramming genes were evaluated. Although DNMT1 and DNMT3α were not differently expressed in two groups, the expression of the one of pluripotent gene, Oct4 was significantly higher in mHMC. In conclusion, based on the comparable results of mMHC and SCNT, the mHMC could be a suitable alternative technique to clone embryos in cost effective way compared to traditional SCNT.

Effects of Selection for High and Low Serum Insulin-like Growth Factor-I on Reproductive Performance in Rams

Eun-Kuk Park,Yun-Sik Lee,Yujing Mi,John. F. Cockrem,Hugh T. Blair,Paul R. Kenyon,Tim. J. Parkinson,Jong Taek Yoon,Jong-Phil Chu 한국동물생명공학회(구 한국동물번식학회) 2008 발생공학 국제심포지엄 및 학술대회 Vol.2008 No.1

Reproductive and Metabolic Endocrinology in Rams Selected for High or Low Plasma IGF-I Concentrations

Eun-Kuk Park,Yun-Sik Lee,Won-Hyung Choi,T. J. Parkinson,J. F. Cockrem,K. S. Han,H. T. Blair,Jong Taek Yoon,Jong-Phil Chu 한국동물생명공학회(구 한국동물번식학회) 2008 발생공학 국제심포지엄 및 학술대회 Vol.2008 No.1

Stability Evaluation of National Reference Standards for Blood Products in Korea

Park, Tae Jun,Choi, Chan Woong,Oh, Ho Kyung,Kim, Jae Ok,Kim, Byung Kuk,Kang, Hyun Kyung,Kwon, Eun Jeong,Gweon, Eun Jeong,Park, Sang Jin,Kang, Ho Il,Jung, Ki Kyung,Park, Sang Mi,Kim, Ji Hye,Han, Ki Won Korean Society of ToxicologyKorea Environmental Mu 2017 Toxicological Research Vol.33 No.3

National reference standards (NRSs) for biologics are established through potency estimation by a multi-center joint study of standard materials used in the approval process for national lot release and quality control of vaccines, blood products, and other biologics. In this study, a stability evaluation was conducted to determine whether the potency of NRSs for six blood products was being maintained at a consistent level in Korea. The present study conducted real-time stability tests via in-vivo/in-vitro bioassay on NRSs for blood coagulation factor VIII concentrate (2nd standard), antithrombin concentrate, prekallikrein activator, anti-hepatitis B immunoglobulin, blood coagulation factor IX concentrate, and anti-tetanus human immunoglobulin, as well as a trend analysis using cumulative annual results. The real-time stability test results showed that the mean potency of six NRSs was all within the control limit. In the trend analysis, the potency of NRS for blood coagulation factor VIII concentrate (2nd standard) showed a decreasing trend, while the potency of all other products had been stably maintained. The present study confirmed that the mean potency of NRSs for six blood products had been stably maintained in Korea. The findings of the present study establish a foundation that can ensure the quality of NRSs for biologics in Korea, and it is expected to make a major contribution to the supply of high-quality biologics.