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어린이 김치 표준화에 대한 연구(1) : 어린이 김치의 담금법 개발 Development of the Preparation of Kimchi for the Children
송영옥,전영수,권명자,빈성미,김은희,문정원,김명 부산대학교 가정대학 1995 家政大學硏究報告 Vol.21 No.-
The purpose of this study is reporting the results of first trial for the standardization of children's kimchi in terms of brining standardization, development for the recipe ad pannel test for the children's kimchi. In order to develop the standard recipe for children's kimchi, our research team have visited kimchi factories, temples and houses located in Pusan and Kyung-Sang Nam Do province. Three kinds of chinese cabbage kimchi (hamyang, haeundae, and jinrae kimchi named by region where we collected) which got the top three highest score in panel testing was selected. The original recipes of these kimchi were sightly modified to prepared the kimchi at laboratory. Among three of them, hamyang kimchi which contained dried anchovy power was selected as a model kimchi for development of the children's kimchi recipe. We find out that the optimal salt concentration of brined cabbage and kimchi for the children's kimchi (sliced style kimchi, approximately 2.5X3cm a piece) that showed the hightest pennel score were approximately 1.5% and 2.0%, respectively. The salt concentration of brined cabbage became 1.5, 1.43, and 1.60% after 60 min at 10% brining solution, 45 min at 12% and 30 min at 15%, respectively. The developed recipe used for the children's kimchi at our team is as follows. The major ingredients used for the preparation of kimchi is brined sliced chinese cabbage (salt concentration is 1.5%) 1kg galic 11g, ginger 5.0g, red pepper powder 20.0g, fermented anchovy juice 25.0g, fermented shrimp juice 5.0g dried anchovy and sea weed extracts 30.0g, anchovy power 2.4g glutinious rice gel 40.0g, starch syrup, 10.0g, and apple juice 22.5g. The total acceptability test of developed children's kimchi (test kimchi) was carried out with 84 of 6th grade student during lunch time at elementary school in Pusan where has a school lunch program. Sixty nine percent of student answered test kimchi is very good, 25% of them said it is fair, and only 6% of students answered they didn't like test kimchil. However, for the kimchi supplied from school at lunch, 52% students answered the kimchi is good, 26% of them said it is fair, and 22% of them said they don't like school supplied kimchi. This result tells us that the test kimchi is better than school supplied kimchi and the test kimchi can be developed further as a children's kimchi.
Garcinia mangostana Suppresses Triacylglycerol Synthesis in Hepatocytes and Enterocytes
Eun-Bin Kwon,Dong-Oh Moon,오은솔,Yu Na Song,Ji-Yoon Park,Hyung Won Ryu,김두영,Young-Won Chin,HyunSunLee,Su Ui Lee,김문옥 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.10
Regulation of diacylglycerol acyltransferase (DGAT) and pancreatic lipase (PL) activities is important in thetreatment of triacylglycerol (TG)-related metabolic diseases. Garcinia mangostana, also known as mangosteen, is a traditionalmedicine ingredient used in the treatment of inflammation in Southeast Asia. In this study, The ethanolic extract of G. mangostana peel inhibited human recombinant DGAT1 and DGAT2, and PL enzyme activities in vitro. The inhibitoryactivity of DGAT1 and DGAT2 enzymes of four representative bioactive substances in mangosteen was confirmed. Inaddition, G. mangostana was confirmed to suppress the serum TG levels in C57 mice by inhibiting the absorption andsynthesis of TG in the gastrointestinal tract. Through this study, it was revealed that G. mangostana extract could be useful forthe prevention and amelioration of TG-related metabolic diseases such as obesity and fatty liver.
Antiviral Effect of Korean Native Bee-honey against Influenza A Virus in A549 Cells
Eun-Bin Kwon,Young Soo Kim,Buyun Kim,Hong Min Choi,Soon Ok Woo,Se-Gun Kim,Jang-Gi Choi 한국양봉학회 2023 韓國養蜂學會誌 Vol.38 No.4
Influenza viruses are responsible for respiratory infections in humans, which results in significant morbidity and mortality. Among the drugs commonly employed to treat influenza virus infections, neuraminidase inhibitors, such as oseltamivir and peramivir, feature pro- minently. However, the emergence of drug-resistant viruses underscores the need for the development of new anti-influenza medications. Korean Native bee-honey has been used for medicinal and food. Korean native bee-honey exhibits pharmacological effects, including antioxidant, anti-inflammatory, and antimicrobial activity; however, an anti-influenza effect of Korean native bee-honey has not been reported. In this study, we determined whether Korean native bee-honey from nine different regions (samples A-I) exhibits antiviral activity in pre-, co-, and posttreatment assays using the green fluorescent protein (GFP)-tagged influenza A/PR/8/34 (A/PR/8/34-GFP) virus. The results indicated that sample G (native bee-honey in Nonsan-si, Chungcheongnam-do) exhibited inhibitory effects in the pre-treatment assay against IAV. Also, samples C(native bee-honey in Boseong-gun, Jeollanam-do) and H(native bee-honey in Muju-gun, Jeollabuk-do) had anti-influenza effect under co-treatment conditions, and samples D (native bee-honey in Gapyeong-gun, Gyeonggi-do) and F (native bee-honey in Hamyang-gun, Gyeongsangnam-do) showed antiviral activity under post-treatment conditions. These results warrant further studies to identify the active ingredients and mechanisms underlying the anti- influenza effect of Korean native bee-honey.
Kwon, Eun-Bin,Kang, Myung-Ji,Kim, Soo-Yeon,Lee, Yong-Moon,Lee, Mi-Kyeong,Yuk, Heung Joo,Ryu, Hyung Won,Lee, Su Ui,Oh, Sei-Ryang,Moon, Dong-Oh,Lee, Hyun-Sun,Kim, Mun-Ock Hindawi 2018 Evidence-based Complementary and Alternative Medic Vol.2018 No.-
<P><I>Zanthoxylum ailanthoides</I> (ZA) has been used as folk medicines in East Asian and recently reported to have several bioactivity; however, the studies of ZA on the regulation of triacylglycerol (TG) biosynthesis have not been elucidated yet. In this study, we examined whether the methanol extract of ZA (ZA-M) could reduce oleic acid- (OA-) induced intracellular lipid accumulation and confirmed its mode of action in HepG2 cells. ZA-M was shown to promote the phosphorylation of AMPK and its upstream LKB1, followed by reduction of lipogenic gene expressions. As a result, treatment of ZA-M blocked de novo TG biosynthesis and subsequently mitigated intracellular neutral lipid accumulation in HepG2 cells. ZA-M also inhibited OA-induced production of reactive oxygen species (ROS) and TNF-<I>α</I>, suggesting that ZA-M possess the anti-inflammatory feature in fatty acid over accumulated condition. Taken together, these results suggest that ZA-M attenuates OA-induced lipid accumulation and inflammation through the activation of LKB1/AMPK signaling pathway in HepG2 cells.</P>
SOHN, EUN JEONG,SHIN, MIN JEA,EUM, WON SIK,KIM, DAE WON,YONG, JI IN,RYU, EUN JI,PARK, JUNG HWAN,CHO, SU BIN,CHA, HYUN JU,KIM, SANG JIN,YEO, HYEON JI,YEO, EUN JI,CHOI, YEON JOO,IM, SEUNG KWON,KWEON, HA Spandidos Publications 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.38 No.1
<P>Oxidative stress-induced apoptosis is associated with neuronal cell death and ischemia. The NOL3 [nucleolar protein 3 (apoptosis repressor with CARD domain)] protein protects against oxidative stress-induced cell death. However, the protective mechanism responsible for this effect as well as the effects of NOL3 against oxidative stress in ischemia remain unclear. Thus, we examined the protective effects of NOL3 protein on hydrogen peroxide (H2O2)-induced oxidative stress and the mechanism responsible for these effects in hippocampal neuronal HT22 cells and in an animal model of forebrain ischemia using Tat-fused NOL3 protein (Tat-NOL3). Purified Tat-NOL3 protein transduced into the H2O2-exposed HT22 cells and inhibited the production of reactive oxygen species (ROS), DNA fragmentation and reduced mitochondrial membrane potential (Delta(Psi m)). In addition, Tat-NOL3 prevented neuronal cell death through the regulation of apoptotic signaling pathways including Bax, Bcl-2, caspase-2, -3 and -8, PARP and p53. In addition, Tat-NOL3 protein transduced into the animal brains and significantly protected against neuronal cell death in the CA1 region of the hippocampus by regulating the activation of microglia and astrocytes. Taken together, these findings demonstrate that Tat-NOL3 protein protects against oxidative stress-induced neuronal cell death by regulating oxidative stress and by acting as an anti-apoptotic protein. Thus, we suggest that Tat-NOL3 represents a potential therapeutic agent for protection against ischemic brain injury.</P>
금화규(Abelmoschus manihot) 꽃 추출물의 In Vitro 피부 미백 및 주름 개선 효능 연구
권현지 ( Hyun-ji Kwon ),범석현 ( Seok-hyun Beom ),현진아 ( Jin-a Hyun ),강은빈 ( Eun-bin Kang ),박하은 ( Ha-eun Park ),한동근 ( Dong-geun Han ),김현정 ( Hyun-jeong Kim ),최은영 ( Eun-young Choi ),안봉전 ( Bong-jeon An ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.4
본 연구는 화장품 소재로서 금화규(Abelmoschus manihot) 꽃의 미백 활성율 및 항주름 활성을 평가하기 위해 수행하였다. 금화규 꽃은 물과 70% 에탄올을 이용하여 추출하였다. Tyrosinase 억제 활성은 물과 70% 에탄올 추출물 모두 농도 의존적으로 억제 활성이 증가 된다고 평가되었으며, B16F10 세포주에서 생존율에 영향을 미치지 않은 농도인 10, 25, 50, 75, 100 μg/mL에서 멜라닌 생합성을 저해하는 것으로 확인되었다. 금화규 꽃의 항주름 효능으로는 collagenase와 elastase의 활성 저해 능력을 측정한 결과 70% 에탄올 추출물이 물 추출물보다 높은 항주름 활성을 보였다. 본 연구 결과를 통하여 금화규 꽃 추출물은 미백 활성과 항주름 활성을 가지는 기능성 화장품 소재로 사용 가능할 것으로 사료된다. In this study, the skin whitening and anti-wrinkle activity of Abelmoschus manihot (A. manihot) flowers were evaluated as a cosmetic material. A. manihot flowers were extracted using water and 70% ethanol. Tyrosinase inhibitory activity was evaluated to increase concentration-dependent inhibitory activity in both water (AMW) and 70% ethanol extracts (AME), and was found to inhibit melanin biosynthesis at concentrations that did not affect survival in B16F10 cell lines at 10, 25, 50, 75, and 100 μg/mL. As a result of measuring the anti-wrinkle effectiveness of A. manihot flowers, 70% ethanol extract (AME) showed higher anti-wrinkle activity than water extract (AMW). Through the results of this study, it is considered that the A. manihot flower extract can be used as a functional cosmetic material with whitening and anti-wrinkle activity.