http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Buyun Kim ),( Sonam Jha ),( Ji Hae Seo ),( Chul-ho Jeong ),( Sooyeun Lee ),( Sangkil Lee ),( Young Ho Seo ),( Byoungduck Park ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.6
Methamphetamine (MA) is one of the most commonly abused drugs in the world by illegal drug users. Addiction to MA is a serious public health problem and effective therapies do not exist to date. It has also been reported that behavior induced by psychostimulants such as MA is related to histone deacetylase (HDAC). MeBib is an HDAC6 inhibitor derived from a benzimidazole scaffold. Many benzimidazole-containing compounds exhibit a wide range of pharmacological activity. In this study, we investigated whether HDAC6 inhibitor MeBib modulates the behavioral response in MA self-administered rats. Our results demonstrated that the number of active lever presses in MA self-administered rats was reduced by pretreatment with MeBib. In the hippocampus of rats, we also found MA administration promotes GluN2B, an NMDA receptor subunit, expression, which results in sequential activation of ERK/CREB/BDNF pathway, however, MeBib abrogated it. Collectively, we suggest that MeBib prevents the MA seeking response induced by MA administration and therefore, represents a potent candidate as an MA addiction inhibitor.
Kim, Buyun,Yoon, Jaewoo,Yoon, Seong Woo,Park, Byoungduck SAGE Publications 2017 Integrative cancer therapies Vol.16 No.2
<P>Cysteine X cysteine (CXC) chemokine receptor 4 (CXCR4) and C-X-C motif chemokine 12 (CXCL12) were originally identified as chemoattractants between immune cells and sites of inflammation. Since studies have validated an increased level of CXCL12 and its receptor in patients with colorectal cancers, CXCL12/CXCR4 axis has been considered as a valuable marker of cancer metastasis. Therefore, identification of CXCR4 inhibitors has great potential to abrogate tumor metastasis. Onbaekwon (OBW) is a complex herbal formula that is derived from the literature of traditional Korean medicine <I>Dongeuibogam</I>. In this study, we demonstrated that OBW suppressed CXCR4 expression in various cancer cell types in a concentration- and time-dependent manner. Both proteasomal and lysosomal inhibitors had no effect to prevent the OBW-induced suppression of CXCR4, suggesting that the inhibitory effect of OBW was not due to proteolytic degradation but occurred at the transcriptional level. Electrophoretic mobility shift assay further confirmed that OBW could block endogenous activation of nuclear factor kappa B, a key transcription factor that regulates the expression of CXCR4 in colon cancer cells. Consistent with the aforementioned molecular basis, OBW abolished cell invasion induced by CXCL12 in colon cancer cells. Together, our results suggest that OBW, as a novel inhibitor of CXCR4, could be a promising therapeutic agent contributing to cancer treatment.</P>
Kim, Buyun,Lee, Ki Yong,Park, Byoungduck Elsevier 2018 Phytomedicine Vol.51 No.-
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Icariin is pharmacologically active prenylated flavonoid glycoside that has various biologic effects such as antioxidant, anticancer, and anti-inflammatory activities. In addition, icariin has been used in Chinese medicine for thousands of years to treat osteoporosis and it is still being used today. However, direct mechanism of icariin in the treatment of bone disease is not understood.</P> <P><B>Purpose</B></P> <P>The purpose of this study is to investigate whether icariin influences RANKL-induced osteoclast formation in murine macrophages.</P> <P><B>Methods</B></P> <P>Osteoclastogenesis was determined by TRAP staining and activity assay. Inhibition of signaling pathways and marker protein expression were evaluated by western blot analysis. The NF-κB (p65) nuclear localization was detected by immunofluorescence assay, and NF-κB/DNA-binding activity was detected by electrophoretic mobility shift assay (EMSA).</P> <P><B>Results</B></P> <P>In our study, icariin inhibited the differentiation of pre-osteoclast cells into osteoclasts and suppressed expression of various genes involved in osteoclast formation and bone resorption. Also, icariin blocked the osteoclastogenesis induced by MCF7 and MDA-MB-231 breast cancer cells through inhibition of NF-κB activation. We found that icariin inhibited RANKL-stimulated TRAF-6 expression, and subsequently suppressed the phosphorylation of ERK, but icariin did not show an effect on p38, JNK, and Akt activation.</P> <P><B>Conclusion</B></P> <P>These results indicate that icariin is likely to be a candidate for bone-related disease treatment and that icariin provides insights into the molecular mechanisms that influence RANKL-induced osteoclast differentiation.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kim, Min-Jung,Koo, Jeong-Eun,Han, Gi-Yeon,Kim, Buyun,Lee, Yoo-Sun,Ahn, Chiyoung,Kim, Chan-Wha The Korean Academy of Medical Sciences 2016 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.31 No.3
<P>Cancer stem cells (CSCs) have tumor initiation, self-renewal, metastasis and chemo-resistance properties in various tumors including colorectal cancer. Targeting of CSCs may be essential to prevent relapse of tumors after chemotherapy. Phosphatidylinositol-3-kinase (PI3K) and mammalian target of rapamycin (mTOR) signals are central regulators of cell growth, proliferation, differentiation, and apoptosis. These pathways are related to colorectal tumorigenesis. This study focused on PI3K and mTOR pathways by inhibition which initiate differentiation of SW620 derived CSCs and investigated its effect on tumor progression. By using rapamycin, LY294002, and NVP-BEZ235, respectively, PI3K and mTOR signals were blocked independently or dually in colorectal CSCs. Colorectal CSCs gained their differentiation property and lost their stemness properties most significantly in dual-blocked CSCs. After treated with anti-cancer drug (paclitaxel) on the differentiated CSCs cell viability, self-renewal ability and differentiation status were analyzed. As a result dual-blocking group has most enhanced sensitivity for anti-cancer drug. Xenograft tumorigenesis assay by using immunodeficiency mice also shows that dual-inhibited group more effectively increased drug sensitivity and suppressed tumor growth compared to single-inhibited groups. Therefore it could have potent anti-cancer effects that dual-blocking of PI3K and mTOR induces differentiation and improves chemotherapeutic effects on SW620 human colorectal CSCs.</P>
Methamphetamine-Induced Neuronal Damage: Neurotoxicity and Neuroinflammation
( Buyun Kim ),( Jangmi Yun ),( Byoungduck Park ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.5
Methamphetamine (METH) is a highly addictive psychostimulant and one of the most widely abused drugs worldwide. The continuous use of METH eventually leads to drug addiction and causes serious health complications, including attention deficit, memory loss and cognitive decline. These neurological complications are strongly associated with METH-induced neurotoxicity and neuroinflammation, which leads to neuronal cell death. The current review investigates the molecular mechanisms underlying METH-mediated neuronal damages. Our analysis demonstrates that the process of neuronal impairment by METH is closely related to oxidative stress, transcription factor activation, DNA damage, excitatory toxicity and various apoptosis pathways. Thus, we reach the conclusion here that METH-induced neuronal damages are attributed to the neurotoxic and neuroinflammatory effect of the drug. This review provides an insight into the mechanisms of METH addiction and contributes to the discovery of therapeutic targets on neurological impairment by METH abuse.
Antiviral Effect of Korean Native Bee-honey against Influenza A Virus in A549 Cells
Eun-Bin Kwon,Young Soo Kim,Buyun Kim,Hong Min Choi,Soon Ok Woo,Se-Gun Kim,Jang-Gi Choi 한국양봉학회 2023 韓國養蜂學會誌 Vol.38 No.4
Influenza viruses are responsible for respiratory infections in humans, which results in significant morbidity and mortality. Among the drugs commonly employed to treat influenza virus infections, neuraminidase inhibitors, such as oseltamivir and peramivir, feature pro- minently. However, the emergence of drug-resistant viruses underscores the need for the development of new anti-influenza medications. Korean Native bee-honey has been used for medicinal and food. Korean native bee-honey exhibits pharmacological effects, including antioxidant, anti-inflammatory, and antimicrobial activity; however, an anti-influenza effect of Korean native bee-honey has not been reported. In this study, we determined whether Korean native bee-honey from nine different regions (samples A-I) exhibits antiviral activity in pre-, co-, and posttreatment assays using the green fluorescent protein (GFP)-tagged influenza A/PR/8/34 (A/PR/8/34-GFP) virus. The results indicated that sample G (native bee-honey in Nonsan-si, Chungcheongnam-do) exhibited inhibitory effects in the pre-treatment assay against IAV. Also, samples C(native bee-honey in Boseong-gun, Jeollanam-do) and H(native bee-honey in Muju-gun, Jeollabuk-do) had anti-influenza effect under co-treatment conditions, and samples D (native bee-honey in Gapyeong-gun, Gyeonggi-do) and F (native bee-honey in Hamyang-gun, Gyeongsangnam-do) showed antiviral activity under post-treatment conditions. These results warrant further studies to identify the active ingredients and mechanisms underlying the anti- influenza effect of Korean native bee-honey.