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Kim, M-J,Kang, J-H,Park, Y G,Ryu, G R,Ko, S H,Jeong, I-K,Koh, K-H,Rhie, D-J,Yoon, S H,Hahn, S J,Kim, M-S,Jo, Y-H Journal of Endocrinology, Ltd. [etc.] 2006 The Journal of endocrinology Vol.188 No.3
<P>Glucagon-like peptide-1 (GLP-1) and its analog exendin-4 (EX) have been considered as a growth factor implicated in pancreatic islet mass increase and beta-cell proliferation. This study aimed to investigate the effect of EX on cyclin D1 expression, a key regulator of the cell cycle, in the pancreatic beta-cell line INS-1. We demonstrated that EX significantly increased cyclin D1 mRNA and subsequently its protein levels. Although EX induced phosphorylation of Raf-1 and extracellular-signal-regulated kinase (ERK), both PD98059 and exogenous ERK1 had no effect on the cyclin D1 induction by EX. Instead, the cAMP-elevating agent forskolin induced cyclin D1 expression remarkably and this response was inhibited by pretreatment with H-89, a protein kinase A (PKA) inhibitor. Promoter analyses revealed that the cAMP-responsive element (CRE) site (at position -48; 5'-TAACGTCA-3') of cyclin D1 gene was required for both basal and EX-induced activation of the cyclin D1 promoter, which was confirmed by site-directed mutagenesis study. For EX to activate the cyclin D1 promoter effectively, CRE-binding protein (CREB) should be phosphorylated and bound to the putative CRE site, according to the results of electrophoretic mobility shift and chromatin immunoprecipitation assays. Lastly, a transfection assay employing constitutively active or dominant-negative CREB expression plasmids clearly demonstrated that CREB was largely involved in both basal and EX-induced cyclin D1 promoter activities. Taken together, EX-induced cyclin D1 expression is largely dependent on the cAMP/PKA signaling pathway, and EX increases the level of phosphorylated CREB and more potently trans-activates cyclin D1 gene through binding of the CREB to the putative CRE site, implicating a potential mechanism underlying beta-cell proliferation by EX.</P>
Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)
양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1
To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.
邊山半島의 植生調査 : 實相寺址 一帶를 中心으로 Mainly of Silsang-sa area
金癸煥,高大植,朴炳益,徐丙秀 전북대학교 농업과학기술연구소 1985 農大論文集 Vol.16 No.-
Vegetation of Silsang-sa area have been investigated in order to give special attention to the plants of botanical value growing in those area as well as the help for the research and study. The plants grown in this area consisted of 3 subdivisions, 5 classes, 26 orders, 51 families, 108 genera, 133 species, 19 varieties and 1 subspecies, 3 formas. In this investigation the community of Abeliophyllum distichum are found at Chungeri valley,
임효근,고순희,박승옥,최예원,김동규,정기석,류지소 대한감염학회 1990 감염 Vol.22 No.4
Pyomyositis is a bacterial infection of skeletal muscle usually occurring in the asence of trauma or a primary focus of infecation. Usual causative agent is Staphylococcus aureus. It is common in the tropics and rare in temperate climates. We diagnosed phomyositis in a 23-year-old man with symptoms of localized muscle pain, fever, chill and malaise. Muscular ultrasonogram and needle aspiration confirmed the disease. It was the first case reported in Korea.
개방형 컨트롤러를 갖는 공작기계에 적합한 진단 및 신호점검사례
김동훈,송준엽,김경돈,김찬봉,김선호,고광식 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-
The conventional computerized numerical controller (CNC) of machine tools has been increasingly replaced by a PC-based open architecture CNC (OAC) which is independent of the CNC vendor. The OAC and machine tools with OAC led to the convenient environment where it is possible to implement user-defined application programs efficiently within CNC. This paper proposes a method of operational fault cause diagnosis which is based on the status of programmable logic controller (PLC) in machine tools with OAC. The operational fault is defined as a disability state occurring during normal operation of machine tools. The faults are occupied by over 70% of all faults and are also unpredictable as most of them occur without any warning. Two diagnosis models, the switching function (SF) and the step switching function (SSF), are proposed in order to diagnose the fault cause quickly and exactly. The cause of an occurring fault is logically diagnosed through a fault diagnosis system (FDS) using the diagnosis models. A suitable interface environment between CNC and developed application modules is constructed in order to implement the diagnostic functions in the CNC domain. The diagnosed results were displayed on a CNC monitor for machine operators and provided to a remote site through a web browser. The result of this research could be a model of the fault cause diagnosis and the remote monitoring for machine tools with OAC.
한충수,고학균,송대빈,서정덕,전홍영 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-
Brown rice characteristics (i.e., moisture content, crack ratio, and hardness) were determined to determine proper conditions of brown rice before milling process and to develope a brown rice conditioning machine. Water was sprayed with the flow rate of 50, 100, and 150 cc/min onto brown rice while the brown rice were falling down with the mass flow rate of 15㎏/tin from the upper to lower hopper of the conditioning machine. After conditioning with different water flow rates of 50, 100, and 150 cc/min, the moisture content was decreased negative exponentially with respect to the time lapse. Initial moisture content of 14.8% of brown rice moisture content was increased to 15.1%, 15.3%, and 15.8% after conditioning with water flow rates of 50, 100, and 150 cc/min, respectively, after 14 hours time lapse. Initial crack ratio of 8.7% of brown rice crack ratios were increased positivee exponentially with respect to the time lapse after conditioning with water flow rates of 50, 100, and 150 cc/min. The hardness of the brown rice was decreased after conditioning with water. Models of variation of the moisture content, crack ratio, and hardness of brown rice respect to the time lapse after conditioning with water was developed using experimental data. 주요 용어(Key words): 현미(Brown rice), 조질(Conditioning), 동할율(Crack ratio) 강도(Hardness).