Visualization of Tumor Angiogenesis Using MR Imaging Contrast Agent Gd-DTPA-anti-VEGF Receptor 2 Antibody Conjugate in a Mouse Tumor Model

전홍영,Hong-Hua Yin,김선희,박성훈,김훈수,윤권하 대한영상의학회 2010 Korean Journal of Radiology Vol.11 No.4

Objective: To visualize tumor angiogenesis using the MRI contrast agent, Gd-DTPA-anti-VEGF receptor 2 antibody conjugate, with a 4.7-Tesla MRI instrument in a mouse model. Materials and Methods: We designed a tumor angiogenesis-targeting T1 contrast agent that was prepared by the bioconjugation of gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA) and an anti-vascular endothelial growth factor receptor-2 (VEGFR2) antibody. The specific binding of the agent complex to cells that express VEGFR2 was examined in cultured murine endothelial cells (MS-1 cells) with a 4.7-Tesla magnetic resonance imaging scanner. Angiogenesis-specific T1 enhancement was imaged with the Gd-DTPA-anti-VEGFR2 antibody conjugate using a CT-26 adenocarcinoma tumor model in eight mice. As a control, the use of the Gd-DTPA-anti-rat immunoglobulin G (Gd-DTPA-anti-rat IgG) was imaged with a tumor model in eight mice. Statistical significance was assessed using the Mann-Whitney test. Tumor tissue was examined by immunohistochemical analysis. Results: The Gd-DTPA-anti-VEGFR2 antibody conjugate showed predominant binding to cultured endothelial cells that expressed a high level of VEGFR2. Signal enhancement was approximately three-fold for in vivo T1-weighted MR imaging with the use of the Gd-DTPA-anti-VEGFR2 antibody conjugate as compared with the Gd-DTPA-rat IgG in the mouse tumor model (p < 0.05). VEGFR2 expression in CT-26 tumor vessels was demonstrated using immunohistochemical staining. Conclusion: MR imaging using the Gd-DTPA-anti-VEGFR2 antibody conjugate as a contrast agent is useful in visualizing noninvasively tumor angiogenesis in a murine tumor model.

Combined Radiofrequency Ablation and Double Anti-Angiogenic Protein Therapy to Increase Coagulation Efficacy: An Experimental Study in a Murine Renal Carcinoma Model

전홍영,유종현,변승재,정창원,김태훈,이영환,윤권하 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.4

To evaluate whether suppression of tumor microvasculature by double anti-angiogenic protein (DAAP) treatment could increase the extent of radiofrequency ablation (RFA)-induced coagulation in a murine renal cell carcinoma model. Renal cell carcinoma cell lines were implanted subcutaneously into 10 nude mice. Four mice received adenoviral DAAP treatment and 6 mice received sterile 0.9% saline solution as DAAP-untreated group. The effect of DAAP was evaluated according to the vascularity by contrast-enhanced ultrasound (CEUS) using microbubbles. Four DAAP-treated mice and 4 DAAP-untreated mice were then treated with RFA, resulting in 3 groups: no-therapy (n = 2), RFA only (n = 4), and RFA combined with DAAP treatment (n = 4). Immediately after RFA, the size of coagulation necrosis and mitochondrial enzyme activity were compared between the groups using analysis of variance (ANOVA) and post hoc test. The contrast enhancement ratio for tumor vascularization on CEUS was significantly lower in the DAAP treated group than in DAAP-untreated group (30.2 ± 9.9% vs. 77.4 ± 17.3%; p = 0.021). After RFA, the mean coagulation diameter was 0 mm for no-therapy group, 6.7 ± 0.7 mm for the RFA only group and 8.5 ± 0.4 mm for the RFA with DAAP group (ANOVA, p < 0.001). The area of viable mitochondria within the tumor was 27.9 ± 3.9% in no-therapy group, 10.3 ± 4.5% in the RFA only group, and 2.1 ± 0.7% in the RFA with DAAP group (ANOVA, p < 0.001). Our results suggest the potential value of combining RFA with anti-angiogenic therapy.

논문 : 농상물,식품가공공학 ; 고주파 저항방식 함수율계의 보정식 개발 및 특성평가

전홍영 ( H. Y. Jeon ),강태환 ( T. H. Kang ),한충수 ( C. S. Han ) 한국농업기계학회 2010 바이오시스템공학 Vol.35 No.3

논문 : 노외기계시스템공학 ; 초음파센서를 이용한 변량제어 스프레이어

전홍영 ( H. Y. Jeon ),주허핑 ( H. Zhu ) 한국농업기계학회 2011 바이오시스템공학 Vol.36 No.1

An experimental variable rate nursery sprayer was developed to adjust application rates for canopy volume in real time. The sprayer consisted of two vertical booms integrated with ultrasonic sensors, and variable rate nozzles coupled with pulse width modulation (PMW) based solenoid valves. A custom-designed microcontroller instructed the sensors to detect canopy size and occurrence and then controlled nozzles to achieve variable application rates. A spray delivery system, which consisted of diaphragm pump, pressure regulator and 4-cycle gasoline engine, offered the spray discharge function. Spray delay time, time adjustment in spray trigger for the leading distance of the sensor, was measured with a high-speed camera, and it was from 50 to 140 ms earlier than the desired time (398 ms) at 3.2 km/h under indoor conditions. Consequently, the sprayer triggered 4.5 to 12.5 cm prior to detected targets. Duty cycles of the sprayer were from 20 to 34 ms for senor-to -canopy (STC) distance from 0.30 to 0.76 m. Outdoor test confirmed that the nozzles were triggered from 290 to 380 ms after detecting tree canopy at 3.2 km/h. The spray rate of the new sprayer was 58.4 to 85.2% of the constant application rate (935 L/ha). Spray coverage was collected at four areas of evergreen canopy by water sensitive papers (WSP), and ranged from 1.9 to 41.1% and 1.8 to 34.7% for variable and constant rate applications, respectively. One WSP area had significant (P < 0.05) difference in mean spray coverage between two application conditions.

논문 : 정보처리 및 복합기술 ; 기계시각을 이용한 분무입자크기 측정

전홍영 ( H. Y. Jeon ),티안레이 ( Lei Tian ) 한국농업기계학회 2010 바이오시스템공학 Vol.35 No.6

A machine vision-based instrument to measure a droplet size spectrum of a spray nozzle was developed and tested to evaluate its accuracy on measuring spray droplet sizes and classifying nozzle sizes. The instrument consisted of a machine vision, light emitting diode (LED) illumination and a desktop computer. The illumination and machine vision were controlled by the computer through a C++ program. The program controlled the machine vision to capture droplet images under controlled illumination, and processed the droplet images to characterize the droplet size distribution of a spray nozzle. An image processing algorithm was developed to improve the accuracy of the system by eliminating random noise and out-of-focus droplets in droplet images while measuring droplet sizes. The instrument measured sizes of the three different balls (254.0, 497.8 and 793.8 μm) and the measurement ranges were 241.2-273.6 μm, 492.9-529.6 μm and 800.8- 824.1 μm for 254.0-, 497.84- and 793.75-μm balls, respectively. Error of the measured droplet mean was less than 3.0 %. Droplet statistics, D(V0.1), D(V0.5) and D(V0.9), of a reference nozzle set were measured, and droplet size spectra of five spray nozzles covering from very fine to extremely coarse were measured to classify spray nozzle sizes. Ninety percent of the classification results of the instrument agreed with manufacturer`s classification. A comparison study was carried out between developed and commercial instruments, and measurement results of the developed instrument were within 20 % of commercial instrument results.

Evaluation of Antioxidant Activities and Active Compounds Separated from Water Soluble Extracts of Korean Black Pine Barks

ChangZhe Shen,전홍영,SungHo Choi,YoungMan Kim,EunJoo Jung,GiSu Oh,SungJin Joo,SungHyun Kim,김일광 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.12

Black pine barks from the southern region of Korea were extracted using pressurized hot water and the water soluble extracts were then separated in a stepwise fashion using a variety of solvents, column chromatography (CC), thin layer chromatography (TLC), and high pressure liquid chromatography (HPLC). The antioxidant activities of each fraction and the active compounds were determined based on the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), reductive potential of ferric ion, and total phenol contents. A DPPH test showed that the half maximal effective concentration (EC50 value : 6.59 ± 0.31 μg/mL) of the ethyl acetate fraction (ca. 0.67%) was almost the same as that of the control compounds and inversely proportional to the value of the total phenol contents. The cell viability of the water extracts was confirmed by methyl thiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) with enzyme linked immune sorbent assay (ELISA). Catechin, epicatechin, quercetin and ferulic acid were isolated from the ethyl acetate fraction as active compounds and identified by nuclear magnetic resonance. The antioxidant activity as value of DPPH of each of the separated compounds was lower than the ethyl acetate fraction, and ferulic acid was the lowest among these compounds.

조질 후 현미의 물성변화와 예측모델개발

한충수,고학균,송대빈,서정덕,전홍영 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-

Brown rice characteristics (i.e., moisture content, crack ratio, and hardness) were determined to determine proper conditions of brown rice before milling process and to develope a brown rice conditioning machine. Water was sprayed with the flow rate of 50, 100, and 150 cc/min onto brown rice while the brown rice were falling down with the mass flow rate of 15㎏/tin from the upper to lower hopper of the conditioning machine. After conditioning with different water flow rates of 50, 100, and 150 cc/min, the moisture content was decreased negative exponentially with respect to the time lapse. Initial moisture content of 14.8% of brown rice moisture content was increased to 15.1%, 15.3%, and 15.8% after conditioning with water flow rates of 50, 100, and 150 cc/min, respectively, after 14 hours time lapse. Initial crack ratio of 8.7% of brown rice crack ratios were increased positivee exponentially with respect to the time lapse after conditioning with water flow rates of 50, 100, and 150 cc/min. The hardness of the brown rice was decreased after conditioning with water. Models of variation of the moisture content, crack ratio, and hardness of brown rice respect to the time lapse after conditioning with water was developed using experimental data. 주요 용어(Key words): 현미(Brown rice), 조질(Conditioning), 동할율(Crack ratio) 강도(Hardness).

고해상도 초음파현미경을 위한 미세기포초음파조영제개발

전세정,김은아,박성훈,이혜진,전홍영,변승재,윤권하 대한영상의학회 2007 대한영상의학회지 Vol.56 No.5

Purpose: To develop optimal microbubble contrast agents (MBCAs) for performing ultrasound microscopy when examining small animals. Materials and Methods: We prepared three types of MBCAs. First, a mixture of three parts of 40% dextran and one part of 5% human serum albumin were sonicated with perfluorocarbon (PFC) (MB1-D40A5P). Second, three parts of 40% dextran and one part of 1% human serum albumin were sonicated with PFC (MB2-D40A1P). Third, all parts of 1% bovine serum albumin were sonicated with PFC (MB3-A1P). We measured the microbubbles' sizes and concentrations with using image analysis software. The acoustic properties of the microbubbles were assessed both in vitro and in vivo. Results: The majority of the MB1-D40A5Ps had a diameter of 2-5 μm, the mean diameter of the MB2-D40A1Ps was 2.5 μm, and the mean diameter of the MB3-A1Ps was less than 2.0 μm. Among the microbubbles, the MB1-D40A5Ps and MB2-D40A1Ps showed increased echogenicity in the abdominal vessels, but the duration of their contrast effect was less than 30 sec. On the contrary, the MB3-A1Ps exhibited strong enhancement in the vessels and their duration was greater than 120 sec. Conclusion: A microbubble contrast agent consisting of all parts of 1% serum albumin sonicated with PFC is an effective contrast agent for ultrasound microscopy. 목적: 작은 동물의 초음파현미경영상검사에 이용할 수 있는 적절한 미세기포초음파조영제를 개발하고자 하였다. 대상과 방법: 세 가지 종류의 미세기포초음파조영제를 개발하여 실험하였다. 첫 번째는 40%의 덱스트란과 5% 혈청알부민을 perfluorocarbon(PFC)에 혼합한 제제(MB1-D40A5P). 두 번째는 40%의 덱스트란과 1% 혈청알부민을 PFC에 혼합한 제제(MB2-D40A5P). 세 번째는 1%의 혈청알부민에 PFC 에 혼합한 제제(MB3-A1P)였다. 이들 세 가지 형태의 미세기포의 크기와 농도를 영상분석소프트웨어로 분석하였고, 초음파현미경을 이용하여 시험관 및 생체영상검사를 시행하였다. 결과: 미세기포조영제의 크기는 90%이상에서 MB1-D40A5P는 2-5 μm, MB2-D40A1P는 2.5 μm, MB3-A1P 2.0μm 이하였다. 이들 조영제중 MB3-A1P 이 다른 조영제보다 강한 조영증강효과를 보였다. MB1-D40A5P과 MB2-D40A1P는 주요복부혈관에서 증가한 조영효과를 보였으나. 조영증가효과가 30초 이내였다. 이에 비해 MB3-A1P는 같은 혈관에서 강한 조영증강효과를 보였으며, 120초 이상의 조영증가효과를 보였다. 결론: 초음파현미경용 미세기포조영제로서 1%의 혈청알부민에 PFC에 혼합한 제제(MB3-A1P)가 효과적으로 이용할 수 있을 가능성이 있는 것으로 보인다.

현미 조질에 관한 연구(Ⅰ) : 조질 후 현미의 물성 변화와 예측모델 Property variation and predicted model of brown rice after conditioning

한충수,연광석,강태환,전홍영,고학균,서정덕,송대빈 한국농업기계학회 2001 바이오시스템공학 Vol.26 No.1

비알콜성 간 질환 동물모델 영상 빅 데이터 구축을 위한 영상데이터 수집 및 사전평가

이기택 ( Gi-taek Lee ),전홍영 ( Hong Young Jun ),김태훈 ( Tae-hoonkim ),장미연 ( Mi Yeon Jang ),김대원 ( Dae Won Kim ),윤권하 ( Kwon-ha Yoon ) 한국정보처리학회 2017 한국정보처리학회 학술대회논문집 Vol.24 No.1

본 연구는 최근 문제가 되고 있는 비알콜성 간 질환에 대한 빅 데이터의 사전 데이터를 만들기 위해 마우스에서 고지방 식이와 Streptozotocin (STZ)로 모델을 제작하였고, 당뇨와 비만 정도를 측정하여 질환발생 정도를 확인하였다. 또한, MR영상의 지속적인 촬영으로 질환발생과정에 대해 3D분석 소프트웨어로 평가되었다.