Inbred 마우스 수정란을 이용한 형질전환마우스 생산에 있어서의 효과적인 수정란 이식 방법에 관한 연구

Wang A. G,S. U. Kim,Y. H. Han,S. K. Kim,D. Y. Yu 한국동물생명공학회(구 한국동물번식학회) 2003 Reproductive & developmental biology Vol.27 No.1

본 연구의 목적은 inbred 마우스 (C57BL/6J)의 수정란을 이용하여 형질전환마우스를 생산할 때, 수정란이식의 효율성을 증진시키기 위한 것이다. C57BL/6J 및 BCF1 마우스로부터 과배란처리 방법에 의해 수정란을 얻고, DNA를 1 세포기 수정란에 미세 주입한 다음, 1세포기 또는 2 세포기의 수정란을 가임신된 마우스의 한쪽 또는 양쪽 난관에 각각 이식하였다. 1세포기의 수정란을 0.75 d.p.c. 가임신된 마우스의 한쪽 난관에 이식했을 때, 임신율이 C57BL/6J는 68.8±7.83%, BCF1은 48.3±14.22% 이었고, 이식한 수정란 당 산자의 발달율은 C57BL/6J가 11.9±5.51%, BCF1은 10.5±8.03%로 성적이 저조하였다. 그러나, 2세포기의 수정란을 0.5 d.p.c. 가임신된 마우스의 양쪽 난관에 이식했을 때, 임신율이 C57BL/6J는 94.4±9.64%, 13CFl은 100±0% 이었고, 이식한 수정란 당 산자의 발달율은 C57BL/6J가 22.1 ±0.4%, BCF1은 21.8±0.38%였다. 따라서 C57BL/6J 마우스의 2세포기 수정란을 0.5 d.p.c. 가임신된 마우스의 양쪽 난관에 이식하는 것이, BCF1마우스와 유사한 성적을 얻어 경쟁력이 있는 것으로 판단되었다. 이러한 결과에 영향을 미치는 인자가 여러 가지 있을 것으로 판단되지만, C57BL/6J 마우스의 2세포기 수정란을 0.5 d.p.c.가임신된 마우스의 양쪽 난관에 이식하는 방법이 다른 방법보다 형질전환마우스를 생산하는데 효율성이 더 높은 것으로 본 실험에서 확인되었다. The objective of this study is to improve the efficiency of embryo transfer in generation of transgenic mice using inbred mouse (C57BL/6J) embryos. The embryos of C57BL/6J and BCF1 mice were superovulated by the standard protocol. One-cell stage of embryos were microinjected and the resulted one-or two-cell were transferred into one-or two-side oviducts of foster mother, respectively. When one-cell stage of embryos were transferred into one-side oviduct of 0.75 d.p.c. foster mother, the results were not ideal because of showing pregnancy ratios of 68.8±7.83% for C57BL/6J and 48.3±14.22% for BCF1, and development ratios of pups vs transferred embryos of 11.9±5.51% for C57BL/6J and 10.5±8.03% fur BCF1 . However, when two-cell stage of embryos were transferred into two-side oviducts of 0.5 d.p.c. foster mother, we got better results of 94.4±9.64% and 100±0% pregnancy ratio, and 22.1±0.4% and 21.8±0.38% development ratio for C57BL/6J and BCF1, respectively. Therefore, transferring two-cell stage of C57BL/6J embryos into two-side oviducts of 0.5 d.p.c. foster mother may be competitive to the result in BCF1 embryos. Even if there are a lot of other factors affecting these results, we conclude from these experiments that transfer of two-cell embryos into two-side oviducts of 0.5 d.p.c. foster mother is a more efficient and safe method than others in generating transgenic mice using inbred mice embryos.

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

Aqueous and dietary bioaccumulation of antibiotic tetracycline in D. magna and its multigenerational transfer

Kim, H.Y.,Jeon, J.,Hollender, J.,Yu, S.,Kim, S.D. Elsevier Scientific Pub. Co 2014 Journal of hazardous materials Vol.279 No.-

The potential bioaccumulation and distribution of antibiotics in non-target organisms have been inadequately studied in spite of their widespread occurrence in aquatic systems. We investigated the ability of tetracycline to bioaccumulate through aqueous and dietary routes in an aquatic organism, the freshwater crustacean Daphnia magna. D. magna was exposed to algal food (Pseudokirchneriella subcapitata) contaminated with tetracycline for dietary uptake. Tetracycline was transferred to D. magna more through aqueous uptake than through dietary uptake. The uptake rate constant of tetracycline for D. magna was k<SUB>in,water</SUB>=0.33+/-0.045 via the aqueous route and k<SUB>in,food</SUB>=0.16+/-0.012 via the dietary route for 1.0mgL<SUP>-1</SUP> tetracycline. Bioconcentration factors of 4.40+/-0.91Lkg<SUP>-1</SUP> and 3.66+/-0.50Lkg<SUP>-1</SUP> for 0.1 and 1.0mgL<SUP>-1</SUP> tetracycline were found for D. magna. The biomagnification factor of 0.19+/-0.04 indicates that magnification of tetracycline through the food web will not occur. The change in the internal concentration of the target compound was also studied for multigenerational (F1-F4) exposure. The internal concentration in D. magna showed a decreasing trend with increasing generations except for the parent generation. The bioaccumulation tendency showed a biphasic change in multigenerational exposure.

혈청 단백 분획치의 참고치 조사(서울 의과학 연구소 서울 임상검사 센타)

유영식 ( Y S Yu ),이병욱 ( B O Lee ),송남석 ( N S Song ),성상철 ( S C Seong ),이경옥 ( K O Lee ),서덕규 ( D K Seo ),이규범 ( K B Lee ) 대한임상검사과학회 1991 대한임상검사과학회지(KJCLS) Vol.23 No.1

A study was performed to compare an agarose gel electrophoresis for the protein fraction with the other reported reference values. It was carried out to determine the reference values of adults in Seoul area, from Oct. 1st 1991 through Oct. 20th 1991. 143 male and 87 female venous blood sampl- es were collected from SCL personnel and collected analized by the agarose gel electrophoresis at the Seoul Clinical Laboratory of Seoul Medical Science Institute. The results are shown on Table 2 and 3. Our results show that albumin was 2. 4% lower and .,-globulin was 4. 5% higher compared with the Corning report in the U.S. A., whereas our results were virtually equal to previously reported results of rural population in Korea. The increase- d y-globulin albeit small, may indicate more prevalent chronic infection among Korea populati on.

Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway

Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27

<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>

노인의 병원 선택 경험

김단비,김민지,김해솔,김희정,박윤선,손유경,송예진,유예림,이다예,이서영,이지현,강숙정,김부연 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: The purpose of this descriptive study was to examine hospital choice factors among elderly patients to understand their experience and use as data to move towards senior-friendly hospitals. Methods: The individual in-depth interviews were conducted from August 13th to 18th, 2019. The participants consisted of eight senior citizens aged 65 and above that suffered from chronic illnesses and had regular hospital visits. Results: This study found that when elderly patients choose the hospital, they considered ‘awareness such as brand name of the hospital', ‘quality of medical service',‘convenience', ‘healthcare team / hospital employee', ‘personal experience', ‘children’s recommendation’ and etc,. The significant point was that all these factors were related to personal experiences from specific hospitals. Conclusion: This study analyzed the hospital choice factors of the elderly patients with high hospital utilization rates and found that the results were mainly affected by the distinct characteristics of elderly patients. The implications of this study are that we proposed further research directions and means for the improvement of the hospital. We suggest hospitals to increase labor allocation for elderly patients with difficulties dealing with unmanned systems such as kiosk and strengthen the role of healthcare providers as instructors for higher satisfaction.

Emergence and Persistence of NS5A and NS3 Resistance-Associated Substitutions in HCV Genotype 1b Patients Treated with Daclatasvir and Asunaprevir

( F. Mcphee ),( D. Hernandez ),( N. Zhou ),( F. Yu ),( B. Kienzle ),( Y. Zhao ),( M. Linaberry ),( S. Noviello ),( M. L. Yu ),( S. H. Ahn ),( Y. Karino ),( K. Chayama ),( H. Kumada ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: A pooled analysis of emergent RAS was performed in HCV genotype (GT-)1b-infected patients receiving daclatasvir and asunaprevir (DCV+ASV) and the persistence of DCV- and ASV-resistant substitutions through ≥post-treatment Week (PTWK)192 was assessed to understand the RAS profile and help guide potential retreatment options. Methods: HCV GT-1b-infected patients without a sustained virologic response (SVR) and with HCV RNA ≥1000 IU/mL on or after DCV+ASV treatment were included from 5 Phase 2 and 3 studies. Baseline and post-baseline plasma samples were sequenced at a sensitivity cut-off ł20%. To determine the persistence of emergent RAS, samples at the end of study (up to PTWK48) and/or from a 3-year long-term follow-up rollover study were sequenced (sensitivity cut-off ≥20%, and ≥1% for select samples). Results: 152 DCV+ASV-treated patients without SVR met the resistance testing criteria: 89% (136/152) had NS5A and 95% (145/152) had NS3 sequences at both baseline and virologic failure (VF). NS5A and NS3 RAS emerged in 99% (134/136) and 89% (129/145), respectively, at VF (Table). Overall, 93% (142/152) of patients with VF had both NS5A and NS3 sequence data at failure, of which 77% (109/142) had RAS at L31, Y93 and D168. Emergent NS5A RAS persisted at PTWK96 (92%;24/26) and ≥PTWK192 (100;7/7compared with 22% (6/27) and 14% (1/7), respectively, for emergent NS3 RAS. Replacement of emergent NS5A and NS3 RAS observed at VF occurred in 8% (2/26) of NS5A and 74% (17/23) of NS3 sequences at PTWK96 and in 0% (0/7) of NS5A and 86% (6/7) of NS3 sequences at ≥PTWK192. Conclusions: NS5A and NS3 RAS emerged in most patients treated with DCV+ASV who experienced VF, and NS5A RAS persisted post-treatment. Therapy options for DCV+ASV treatment failures may depend on the timing of retreatment: an NS3 inhibitor-containing regimen may be possible if NS3 RAS are no longer observed, while regimens not impacted by the NS5A-L31+Y93 and NS3-D168 RAS combination would offer an immediate alternative.

BELLE TOF 검출기의 특성 연구

남지우,김동욱,배미영,박강순,김정현,노상률,최영일,김희종,장동욱,최소명,권영준,Hikita, S.,Yu, C.,Yoshimura, Y.,Kichimi, H. 성균관대학교 기초과학연구소 1998 論文集 Vol.49 No.-

We investigated the properties of BELLE Time Of Flight(TOF) counter using the data taken in Jan 98 beam test at Pi2 beam line at KEK 12 GeV proton synchrotron. Charge distributions from readout PMT were analysed in comparison with dE/dx distributions predicted by GEANT full simulation. Experimental setup of the beam test is described, then procedures of data analysis, Monte Carlo simulation and analysis results are also described.

20대 여성의 데이트폭력 피해 경험과 우울, 불안 및 신체화의 관계

김수민,김예진,김유정,남지현,박금빈,박이준,안혜민,오세연,유가현,유다현,전예지,김옥수,길민지 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: The purpose of this study is to examine the degree of date violence damage, depression, anxiety, and somatization among women in their 20s who had experienced date violence, and to investigate the correlations b etween the subjects’ experience of date violence damage and depression, anxiety and somatization. Method: The subject of this study were collected from September 04 to October 13, 2019, by posting advertisements on the women’s university community site in Seoul and a representative women;s community site on the Internet. 149 subjects were collected in their 20s who had experienced date violence from their partners. SPSS statistics 22.0 statistical program was used for data analysis, and standard deviation, T-test, ANOVA, and Pearson’s correlation coefficients were performed. Result: The results showed that the degree of experience of date violence was significantly correlated with symptoms of depression, anxiety, and somatization. Conclusion: Since there was correlations b etween depression, anxiety, and somatization symptoms in date violence victims, it is necessary to apply nursing intervention related to depression and anxiety to prevent somatization symptoms caused by date violence.

Inhibitory effect of fenofibrate on neointima hyperplasia via G₀/G₁ arrest of cell proliferation

Lee, J.J.,Yu, J.Y.,Zhang, W.Y.,Kim, T.J.,Lim, Y.,Kwon, J.S.,Kim, D.W.,Myung, C.S.,Yun, Y.P. North-Holland ; Elsevier Science Ltd 2011 european journal of pharmacology Vol.650 No.1

We have previously reported that fenofibrate displayed a potent antithrombotic effect by the inhibition of platelet aggregation. The present study was designed to investigate the effects of fenofibrate on the neointimal hyperplasia and its possible molecular mechanism. Neointimal hyperplasia was measured in balloon-inflated-induced vascular injury model of male Sprague-Dawley rats and cell proliferation was measured in primary cultured rat aortic vascular smooth muscle cells (VSMCs). Fenofibrate-treated group showed a significant reduction in neointimal formation (0.07+/-0.04mm<SUP>2</SUP>) from the control (0.13+/-0.04mm<SUP>2</SUP>). Fenofibrate significantly inhibited platelet-derived growth factor (PDGF)-BB-induced cell counting and [<SUP>3</SUP>H]-thymidine incorporation into DNA. Fenofibrate suppressed the PDGF-BB-inducible progression through G<SUB>0</SUB>/G<SUB>1</SUB> to S phase of cell cycle. Moreover, fenofibrate inhibited not only phosphorylation of retinoblastoma (Rb) protein and expression of cyclin D/E, CDK 2/4 and proliferating cell nuclear antigen (PCNA) proteins but also mitogen-activated protein kinase (MAPK) signaling pathways such as ERK ½, p38 and JNK phosphorylation. In conclusion, the present study demonstrates that fenofibrate significantly inhibits neointimal formation via G<SUB>0</SUB>/G<SUB>1</SUB> arrest of PDGF-BB-induced cell proliferation in association with the inhibition of MAPK, which resulted in the downregulation of expressions of cyclin D/E, CDK 2/4 and PCNA proteins, suggesting that fenofibrate may be useful for individuals with a high risk of thrombotic or cardiovascular diseases.